Eikelboom's morphotype 0803 in activated sludge belongs to the genus Caldilinea in the phylum Chloroflexi
Abstract Micromanipulated filamentous bacteria from bulking and foaming activated sludge morphologically identified as Eikelboom type 0803 were shown to be affiliated to the genus Caldilinea within the phylum Chloroflexi. Specific FISH probes were designed for their in situ detection and quantificat...
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| Veröffentlicht in: | FEMS microbiology ecology 2011-06, Vol.76 (3), p.451-462 |
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| creator | Kragelund, Caroline Thomsen, Trine Rolighed Mielczarek, Artur Tomasz Nielsen, Per Halkjær |
| description | Abstract
Micromanipulated filamentous bacteria from bulking and foaming activated sludge morphologically identified as Eikelboom type 0803 were shown to be affiliated to the genus Caldilinea within the phylum Chloroflexi. Specific FISH probes were designed for their in situ detection and quantification in seven Danish wastewater treatment plants with biological nutrient removal. The survey applied all species-specific probes for Chloroflexi of relevance in activated sludge treatment plants as well as the phylum-specific probes. Type 0803 filaments constituted around 20% of the total Chloroflexi population. In four of the treatment plants, type 0803 and type 0092 co-occurred and were the dominating fraction of the Chloroflexi population. In the other plants, most Chloroflexi could not be identified beyond the phylum level, suggesting a yet far larger diversity. On average, for all plants, the total Chloroflexi population constituted 12% of the entire microbial population and seems to play an important structural role in the sludge floc formation. Ecophysiological characterization of type 0803 showed their potential role in macromolecule conversion as evident by high levels of exoenzyme expression. Acetate was not consumed. Glucose was consumed with oxygen, nitrite and nitrite as electron acceptors, suggesting that type 0803 may be a denitrifier. Their surfaces were hydrophobic, explaining their occasional occurrence in foaming incidents. |
| doi_str_mv | 10.1111/j.1574-6941.2011.01065.x |
| format | Article |
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Micromanipulated filamentous bacteria from bulking and foaming activated sludge morphologically identified as Eikelboom type 0803 were shown to be affiliated to the genus Caldilinea within the phylum Chloroflexi. Specific FISH probes were designed for their in situ detection and quantification in seven Danish wastewater treatment plants with biological nutrient removal. The survey applied all species-specific probes for Chloroflexi of relevance in activated sludge treatment plants as well as the phylum-specific probes. Type 0803 filaments constituted around 20% of the total Chloroflexi population. In four of the treatment plants, type 0803 and type 0092 co-occurred and were the dominating fraction of the Chloroflexi population. In the other plants, most Chloroflexi could not be identified beyond the phylum level, suggesting a yet far larger diversity. On average, for all plants, the total Chloroflexi population constituted 12% of the entire microbial population and seems to play an important structural role in the sludge floc formation. Ecophysiological characterization of type 0803 showed their potential role in macromolecule conversion as evident by high levels of exoenzyme expression. Acetate was not consumed. Glucose was consumed with oxygen, nitrite and nitrite as electron acceptors, suggesting that type 0803 may be a denitrifier. Their surfaces were hydrophobic, explaining their occasional occurrence in foaming incidents.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/j.1574-6941.2011.01065.x</identifier><identifier>PMID: 21299573</identifier><identifier>CODEN: FMECEZ</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Activated sludge ; Animal, plant and microbial ecology ; Bacteria ; Biological and medical sciences ; Biological wastewater treatment ; bulking ; Chloroflexi - classification ; Chloroflexi - genetics ; Chloroflexi - metabolism ; DNA Probes - genetics ; DNA, Bacterial - genetics ; Ecology ; filamentous Chloroflexi ; Foaming ; Fundamental and applied biological sciences. Psychology ; In Situ Hybridization, Fluorescence ; microautoradiography ; Microbial ecology ; Microbiology ; Nutrient removal ; Phylogeny ; Population ; Probes ; Sewage - microbiology ; Sludge ; Sludge treatment ; surface properties ; type 0803 ; Wastewater treatment plants ; Water treatment</subject><ispartof>FEMS microbiology ecology, 2011-06, Vol.76 (3), p.451-462</ispartof><rights>2011 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved 2011</rights><rights>2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2015 INIST-CNRS</rights><rights>2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5695-547cddb12eb249316d7ec2d6681f6fdd9f99bee599f73f18ce96d6501efa4c623</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6941.2011.01065.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6941.2011.01065.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24161713$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21299573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kragelund, Caroline</creatorcontrib><creatorcontrib>Thomsen, Trine Rolighed</creatorcontrib><creatorcontrib>Mielczarek, Artur Tomasz</creatorcontrib><creatorcontrib>Nielsen, Per Halkjær</creatorcontrib><title>Eikelboom's morphotype 0803 in activated sludge belongs to the genus Caldilinea in the phylum Chloroflexi</title><title>FEMS microbiology ecology</title><addtitle>FEMS Microbiol Ecol</addtitle><description>Abstract
Micromanipulated filamentous bacteria from bulking and foaming activated sludge morphologically identified as Eikelboom type 0803 were shown to be affiliated to the genus Caldilinea within the phylum Chloroflexi. Specific FISH probes were designed for their in situ detection and quantification in seven Danish wastewater treatment plants with biological nutrient removal. The survey applied all species-specific probes for Chloroflexi of relevance in activated sludge treatment plants as well as the phylum-specific probes. Type 0803 filaments constituted around 20% of the total Chloroflexi population. In four of the treatment plants, type 0803 and type 0092 co-occurred and were the dominating fraction of the Chloroflexi population. In the other plants, most Chloroflexi could not be identified beyond the phylum level, suggesting a yet far larger diversity. On average, for all plants, the total Chloroflexi population constituted 12% of the entire microbial population and seems to play an important structural role in the sludge floc formation. Ecophysiological characterization of type 0803 showed their potential role in macromolecule conversion as evident by high levels of exoenzyme expression. Acetate was not consumed. Glucose was consumed with oxygen, nitrite and nitrite as electron acceptors, suggesting that type 0803 may be a denitrifier. Their surfaces were hydrophobic, explaining their occasional occurrence in foaming incidents.</description><subject>Activated sludge</subject><subject>Animal, plant and microbial ecology</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Biological wastewater treatment</subject><subject>bulking</subject><subject>Chloroflexi - classification</subject><subject>Chloroflexi - genetics</subject><subject>Chloroflexi - metabolism</subject><subject>DNA Probes - genetics</subject><subject>DNA, Bacterial - genetics</subject><subject>Ecology</subject><subject>filamentous Chloroflexi</subject><subject>Foaming</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>microautoradiography</subject><subject>Microbial ecology</subject><subject>Microbiology</subject><subject>Nutrient removal</subject><subject>Phylogeny</subject><subject>Population</subject><subject>Probes</subject><subject>Sewage - microbiology</subject><subject>Sludge</subject><subject>Sludge treatment</subject><subject>surface properties</subject><subject>type 0803</subject><subject>Wastewater treatment plants</subject><subject>Water treatment</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U-L1DAYBvAgijuufgUJiOypY97mT5uDBxlmVVjxoueQNm9nMqZNbVqd-fa2zriCIuaSkPyeQPIQQoGtYR6vDmuQhciUFrDOGcCaAVNyfXxAVvcHD8mKgSozJbS6Ik9SOjAGkgv2mFzlkGstC74ifuu_YKhibG8SbePQ7-N46pGyknHqO2rr0X-zIzqawuR2SCsMsdslOkY67pHusJsS3djgfPAd2iWz7Pf7U5hautmHOMQm4NE_JY8aGxI-u8zX5PPt9tPmXXb38e37zZu7rJZKy0yKonaughyrXGgOyhVY506pEhrVOKcbrStEqXVT8AbKGrVySjLAxopa5fya3Jzv7Yf4dcI0mtanGkOwHcYpmbKYkeAg_i-VBA1cl7N88Yc8xGno5mcYkFDkqlCSz-r5RU1Vi870g2_tcDK_fnsGLy_AptqGZrBd7dNvJ0BBAYt7fXbffcDT_Tkws7RvDmYp2Swlm6V987N9czS32w_Las7zcz5O_T_S2V9p_gOGTbBe</recordid><startdate>201106</startdate><enddate>201106</enddate><creator>Kragelund, Caroline</creator><creator>Thomsen, Trine Rolighed</creator><creator>Mielczarek, Artur Tomasz</creator><creator>Nielsen, Per Halkjær</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7QH</scope><scope>7ST</scope><scope>7UA</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope><scope>SOI</scope></search><sort><creationdate>201106</creationdate><title>Eikelboom's morphotype 0803 in activated sludge belongs to the genus Caldilinea in the phylum Chloroflexi</title><author>Kragelund, Caroline ; Thomsen, Trine Rolighed ; Mielczarek, Artur Tomasz ; Nielsen, Per Halkjær</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5695-547cddb12eb249316d7ec2d6681f6fdd9f99bee599f73f18ce96d6501efa4c623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Activated sludge</topic><topic>Animal, plant and microbial ecology</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Biological wastewater treatment</topic><topic>bulking</topic><topic>Chloroflexi - classification</topic><topic>Chloroflexi - genetics</topic><topic>Chloroflexi - metabolism</topic><topic>DNA Probes - genetics</topic><topic>DNA, Bacterial - genetics</topic><topic>Ecology</topic><topic>filamentous Chloroflexi</topic><topic>Foaming</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>microautoradiography</topic><topic>Microbial ecology</topic><topic>Microbiology</topic><topic>Nutrient removal</topic><topic>Phylogeny</topic><topic>Population</topic><topic>Probes</topic><topic>Sewage - microbiology</topic><topic>Sludge</topic><topic>Sludge treatment</topic><topic>surface properties</topic><topic>type 0803</topic><topic>Wastewater treatment plants</topic><topic>Water treatment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kragelund, Caroline</creatorcontrib><creatorcontrib>Thomsen, Trine Rolighed</creatorcontrib><creatorcontrib>Mielczarek, Artur Tomasz</creatorcontrib><creatorcontrib>Nielsen, Per Halkjær</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Water Resources Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Environment Abstracts</collection><jtitle>FEMS microbiology ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kragelund, Caroline</au><au>Thomsen, Trine Rolighed</au><au>Mielczarek, Artur Tomasz</au><au>Nielsen, Per Halkjær</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Eikelboom's morphotype 0803 in activated sludge belongs to the genus Caldilinea in the phylum Chloroflexi</atitle><jtitle>FEMS microbiology ecology</jtitle><addtitle>FEMS Microbiol Ecol</addtitle><date>2011-06</date><risdate>2011</risdate><volume>76</volume><issue>3</issue><spage>451</spage><epage>462</epage><pages>451-462</pages><issn>0168-6496</issn><eissn>1574-6941</eissn><coden>FMECEZ</coden><abstract>Abstract
Micromanipulated filamentous bacteria from bulking and foaming activated sludge morphologically identified as Eikelboom type 0803 were shown to be affiliated to the genus Caldilinea within the phylum Chloroflexi. Specific FISH probes were designed for their in situ detection and quantification in seven Danish wastewater treatment plants with biological nutrient removal. The survey applied all species-specific probes for Chloroflexi of relevance in activated sludge treatment plants as well as the phylum-specific probes. Type 0803 filaments constituted around 20% of the total Chloroflexi population. In four of the treatment plants, type 0803 and type 0092 co-occurred and were the dominating fraction of the Chloroflexi population. In the other plants, most Chloroflexi could not be identified beyond the phylum level, suggesting a yet far larger diversity. On average, for all plants, the total Chloroflexi population constituted 12% of the entire microbial population and seems to play an important structural role in the sludge floc formation. Ecophysiological characterization of type 0803 showed their potential role in macromolecule conversion as evident by high levels of exoenzyme expression. Acetate was not consumed. Glucose was consumed with oxygen, nitrite and nitrite as electron acceptors, suggesting that type 0803 may be a denitrifier. Their surfaces were hydrophobic, explaining their occasional occurrence in foaming incidents.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21299573</pmid><doi>10.1111/j.1574-6941.2011.01065.x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford Journals Open Access Collection; MEDLINE; Wiley Online Library Journals Frontfile Complete; EZB-FREE-00999 freely available EZB journals |
| subjects | Activated sludge Animal, plant and microbial ecology Bacteria Biological and medical sciences Biological wastewater treatment bulking Chloroflexi - classification Chloroflexi - genetics Chloroflexi - metabolism DNA Probes - genetics DNA, Bacterial - genetics Ecology filamentous Chloroflexi Foaming Fundamental and applied biological sciences. Psychology In Situ Hybridization, Fluorescence microautoradiography Microbial ecology Microbiology Nutrient removal Phylogeny Population Probes Sewage - microbiology Sludge Sludge treatment surface properties type 0803 Wastewater treatment plants Water treatment |
| title | Eikelboom's morphotype 0803 in activated sludge belongs to the genus Caldilinea in the phylum Chloroflexi |
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