Positron emission tomography quantification of [ C]-harmine binding to monoamine oxidase-A in the human brain

This article describes the kinetic modeling of [11C]-harmine binding to monoamine oxidase A (MAO-A) binding sites in the human brain using positron emission tomography (PET). Positron emission tomography studies were performed in healthy volunteers at placebo conditions and after treatment with clinical doses of moclobemide. In either condition, a two-tissue compartment model (2CM) provided better fits to the data than a one-tissue model. Estimates of k3/k4 values from an unconstrained 2CM were highly variable. In contrast, estimates of the specifically bound radioligand distribution volume (DVB) from an unconstrained 2CM were exceptionally stable, correlated well with the known distribution of MAO-A in the brain (cerebellum