Surface display of human lactoferrin using a glycosylphosphatidylinositol-anchored protein of Saccharomyces cerevisiae in Pichia pastoris

A cell surface display system was developed in Pichia pastoris using the gene TIP1, encoding the glycosylphosphatidylinositol (GPI)-anchored protein of Saccharomyces cerevisiae (ScTIP). Human lactoferrin cDNA (hLf) was fused to a full-length TIP1 DNA (ScTIP ₆₃₀ ) or a short-TIP1 fragment (ScTIP ₁₂₀...

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Veröffentlicht in:	Biotechnology letters 2011-06, Vol.33 (6), p.1113-1120
Hauptverfasser:	Jo, Jae-Hyung, Im, Eun-Mi, Kim, Seung-Hwan, Lee, Hyune-Hwan
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Anti-Bacterial Agents - pharmacology antibacterial properties Applied Microbiology Artificial Gene Fusion Base Sequence Biochemistry Biological and medical sciences Biomedical and Life Sciences Biotechnology Carrier Proteins - genetics Carrier Proteins - metabolism Cell Membrane - metabolism Cellular biology complementary DNA DNA Primers - genetics Encoding flow cytometry fluorescent antibody technique Fundamental and applied biological sciences. Psychology Genes Genes, Fungal Genetic Vectors Genetics Glycoproteins - genetics Glycoproteins - metabolism GPI-Linked Proteins - genetics GPI-Linked Proteins - metabolism Humans lactoferrin Lactoferrin - genetics Lactoferrin - metabolism Lactoferrin - pharmacology Life Sciences Microbiology Microorganisms microscopy Original Research Paper Pichia - genetics Pichia - metabolism Pichia pastoris Proteins Recombinant Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Recombinant Fusion Proteins - pharmacology Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Staphylococcus aureus - drug effects Surface chemistry Triton Urea Western blotting Yeast
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