The effectiveness of stilbenes in resistant Vitaceae: Ultrastructural and biochemical events during Plasmopara viticola infection process
Leaves of different Vitis vinifera L. cultivars, susceptible or resistant to downy mildew, Chasselas, Solaris, IRAC 2091 (cvs. Gamaret x Bronner) and Muscadinia rotundifolia were inoculated with Plasmopara viticola. Samples were then examined by scanning and transmission electron microscopy, by ligh...
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| Veröffentlicht in: | Plant physiology and biochemistry 2011-03, Vol.49 (3), p.265-274 |
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| creator | Alonso-Villaverde, Virginia Voinesco, Francine Viret, Olivier Spring, Jean-Laurent Gindro, Katia |
| description | Leaves of different
Vitis vinifera L. cultivars, susceptible or resistant to downy mildew, Chasselas, Solaris, IRAC 2091 (cvs. Gamaret x Bronner) and
Muscadinia rotundifolia were inoculated with
Plasmopara viticola. Samples were then examined by scanning and transmission electron microscopy, by light microscopy and for their ability to synthesise stilbenes. These phytoalexins were strictly analysed at infection sites. In the susceptible Chasselas,
P. viticola colonises, at 72
h post-infection (hpi), all of the spongy mesophyll with functional haustoria and produces mainly the non toxic piceide. No necrotic zone was observed on Chasselas leaves. The ultrastructural response to downy mildew infection is different in each of the other three resistant grape cultivars. In Solaris, where leaf necrosis are rapidly induced, the infection is restricted to the upper part of the loose spongy mesophyll, and associated with a rapid cell wall disruption and the dispersion of cytoplasmic content along with the production of viniferins. In IRAC 2091, leaf necrosis are quite similar to those observed on Solaris but the infected plant cell, as well as the haustoria, show high electron dense cellular particles without any recognisable organelles, probably related to the effect of the toxic compound pterostilbene, which is synthesised in this grape cultivar. In
M. rotundifolia leaf necrosis are much more scarce and smaller than in other cultivars, but pathogen and plant cells are both strongly affected, with concomitant expulsion of cytoplasmic materials through the stomata after
P. viticola penetration. In this cultivar, the concentration of all identified stilbenes exceeds 1
×
10
3
μmol
mg
−1 FW. The critical role of stilbenes in the resistance of
Vitis spp. is discussed.
► Stilbens synthesis is the most effective way to stop downy mildew development in grapes. ► Resistant vines produce high concentrations of toxic stilbens at the sites of infection. ► Functional haustoria formation is not the key-step for the induction of stilbens synthesis. ► Stilben synthesis can occur in all species of the
Vitaceae family. |
| doi_str_mv | 10.1016/j.plaphy.2010.12.010 |
| format | Article |
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Vitis vinifera L. cultivars, susceptible or resistant to downy mildew, Chasselas, Solaris, IRAC 2091 (cvs. Gamaret x Bronner) and
Muscadinia rotundifolia were inoculated with
Plasmopara viticola. Samples were then examined by scanning and transmission electron microscopy, by light microscopy and for their ability to synthesise stilbenes. These phytoalexins were strictly analysed at infection sites. In the susceptible Chasselas,
P. viticola colonises, at 72
h post-infection (hpi), all of the spongy mesophyll with functional haustoria and produces mainly the non toxic piceide. No necrotic zone was observed on Chasselas leaves. The ultrastructural response to downy mildew infection is different in each of the other three resistant grape cultivars. In Solaris, where leaf necrosis are rapidly induced, the infection is restricted to the upper part of the loose spongy mesophyll, and associated with a rapid cell wall disruption and the dispersion of cytoplasmic content along with the production of viniferins. In IRAC 2091, leaf necrosis are quite similar to those observed on Solaris but the infected plant cell, as well as the haustoria, show high electron dense cellular particles without any recognisable organelles, probably related to the effect of the toxic compound pterostilbene, which is synthesised in this grape cultivar. In
M. rotundifolia leaf necrosis are much more scarce and smaller than in other cultivars, but pathogen and plant cells are both strongly affected, with concomitant expulsion of cytoplasmic materials through the stomata after
P. viticola penetration. In this cultivar, the concentration of all identified stilbenes exceeds 1
×
10
3
μmol
mg
−1 FW. The critical role of stilbenes in the resistance of
Vitis spp. is discussed.
► Stilbens synthesis is the most effective way to stop downy mildew development in grapes. ► Resistant vines produce high concentrations of toxic stilbens at the sites of infection. ► Functional haustoria formation is not the key-step for the induction of stilbens synthesis. ► Stilben synthesis can occur in all species of the
Vitaceae family.</description><identifier>ISSN: 0981-9428</identifier><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/j.plaphy.2010.12.010</identifier><identifier>PMID: 21256040</identifier><identifier>CODEN: PPBIEX</identifier><language>eng</language><publisher>Paris: Elsevier Masson SAS</publisher><subject>Biological and medical sciences ; Cytoplasm - ultrastructure ; Downy mildew ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Grapevine ; Leaf ; Necrosis ; Oomycetes ; Phytopathology. Animal pests. Plant and forest protection ; Plant Diseases - immunology ; Plant Diseases - microbiology ; Plant Immunity - physiology ; Plant Leaves - metabolism ; Plant Leaves - ultrastructure ; Plant physiology and development ; Plant Stomata - ultrastructure ; Plasmopara viticola ; Resistance ; Sesquiterpenes - metabolism ; Species Specificity ; Stilbenes ; Stilbenes - metabolism ; Stomata ; Ultrastructure ; Vitaceae ; Vitis ; Vitis - classification ; Vitis - metabolism ; Vitis - ultrastructure ; Vitis vinifera</subject><ispartof>Plant physiology and biochemistry, 2011-03, Vol.49 (3), p.265-274</ispartof><rights>2011 Elsevier Masson SAS</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Masson SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-6dfd185ddff49c4d9e1b89ca63cce9063f48445a2bd995c4a63a3cf6f98a0d8e3</citedby><cites>FETCH-LOGICAL-c469t-6dfd185ddff49c4d9e1b89ca63cce9063f48445a2bd995c4a63a3cf6f98a0d8e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S098194281000272X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23932928$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21256040$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alonso-Villaverde, Virginia</creatorcontrib><creatorcontrib>Voinesco, Francine</creatorcontrib><creatorcontrib>Viret, Olivier</creatorcontrib><creatorcontrib>Spring, Jean-Laurent</creatorcontrib><creatorcontrib>Gindro, Katia</creatorcontrib><title>The effectiveness of stilbenes in resistant Vitaceae: Ultrastructural and biochemical events during Plasmopara viticola infection process</title><title>Plant physiology and biochemistry</title><addtitle>Plant Physiol Biochem</addtitle><description>Leaves of different
Vitis vinifera L. cultivars, susceptible or resistant to downy mildew, Chasselas, Solaris, IRAC 2091 (cvs. Gamaret x Bronner) and
Muscadinia rotundifolia were inoculated with
Plasmopara viticola. Samples were then examined by scanning and transmission electron microscopy, by light microscopy and for their ability to synthesise stilbenes. These phytoalexins were strictly analysed at infection sites. In the susceptible Chasselas,
P. viticola colonises, at 72
h post-infection (hpi), all of the spongy mesophyll with functional haustoria and produces mainly the non toxic piceide. No necrotic zone was observed on Chasselas leaves. The ultrastructural response to downy mildew infection is different in each of the other three resistant grape cultivars. In Solaris, where leaf necrosis are rapidly induced, the infection is restricted to the upper part of the loose spongy mesophyll, and associated with a rapid cell wall disruption and the dispersion of cytoplasmic content along with the production of viniferins. In IRAC 2091, leaf necrosis are quite similar to those observed on Solaris but the infected plant cell, as well as the haustoria, show high electron dense cellular particles without any recognisable organelles, probably related to the effect of the toxic compound pterostilbene, which is synthesised in this grape cultivar. In
M. rotundifolia leaf necrosis are much more scarce and smaller than in other cultivars, but pathogen and plant cells are both strongly affected, with concomitant expulsion of cytoplasmic materials through the stomata after
P. viticola penetration. In this cultivar, the concentration of all identified stilbenes exceeds 1
×
10
3
μmol
mg
−1 FW. The critical role of stilbenes in the resistance of
Vitis spp. is discussed.
► Stilbens synthesis is the most effective way to stop downy mildew development in grapes. ► Resistant vines produce high concentrations of toxic stilbens at the sites of infection. ► Functional haustoria formation is not the key-step for the induction of stilbens synthesis. ► Stilben synthesis can occur in all species of the
Vitaceae family.</description><subject>Biological and medical sciences</subject><subject>Cytoplasm - ultrastructure</subject><subject>Downy mildew</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Grapevine</subject><subject>Leaf</subject><subject>Necrosis</subject><subject>Oomycetes</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant Diseases - immunology</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Immunity - physiology</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Leaves - ultrastructure</subject><subject>Plant physiology and development</subject><subject>Plant Stomata - ultrastructure</subject><subject>Plasmopara viticola</subject><subject>Resistance</subject><subject>Sesquiterpenes - metabolism</subject><subject>Species Specificity</subject><subject>Stilbenes</subject><subject>Stilbenes - metabolism</subject><subject>Stomata</subject><subject>Ultrastructure</subject><subject>Vitaceae</subject><subject>Vitis</subject><subject>Vitis - classification</subject><subject>Vitis - metabolism</subject><subject>Vitis - ultrastructure</subject><subject>Vitis vinifera</subject><issn>0981-9428</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9uEzEQxi0EomnhDRDyBXHaYHu9G5tDJRRBQaoEh5arNWuPiaP9h-2N1EfgrXFIoDdOo7F_M9_o-wh5xdmaM96-26_nHubdw1qw45NYl_KErLja1JVoNXtKVkwrXmkp1AW5TGnPGBNyUz8nF4KLpmWSrcivux1S9B5tDgccMSU6eZpy6LtjR8NII6aQMoyZfg8ZLAK-p_d9jpByXGxeIvQURke7MNkdDsGWHsuunKhbYhh_0G89pGGaIQI9hBzs1ENZ_EdzGukcJ1t0X5BnHvqEL8_1itx_-ni3_Vzdfr35sv1wW1nZ6ly1zjuuGue8l9pKp5F3Sltoa2tRs7b2UknZgOic1o2V5QNq61uvFTCnsL4ib097i-7PBVM2Q0gW-x5GnJZk1KYVouFaF1KeSBunlCJ6M8cwQHwwnJljBmZvThmYYwaGC1NKGXt9Fli6Ad2_ob-mF-DNGYBUzPIRRhvSI1frWmihCnd94rDYcQgYTbIBR4suxOKdcVP4_yW_AdZSq3Y</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Alonso-Villaverde, Virginia</creator><creator>Voinesco, Francine</creator><creator>Viret, Olivier</creator><creator>Spring, Jean-Laurent</creator><creator>Gindro, Katia</creator><general>Elsevier Masson SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope></search><sort><creationdate>20110301</creationdate><title>The effectiveness of stilbenes in resistant Vitaceae: Ultrastructural and biochemical events during Plasmopara viticola infection process</title><author>Alonso-Villaverde, Virginia ; Voinesco, Francine ; Viret, Olivier ; Spring, Jean-Laurent ; Gindro, Katia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-6dfd185ddff49c4d9e1b89ca63cce9063f48445a2bd995c4a63a3cf6f98a0d8e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biological and medical sciences</topic><topic>Cytoplasm - ultrastructure</topic><topic>Downy mildew</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Grapevine</topic><topic>Leaf</topic><topic>Necrosis</topic><topic>Oomycetes</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant Diseases - immunology</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Immunity - physiology</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Leaves - ultrastructure</topic><topic>Plant physiology and development</topic><topic>Plant Stomata - ultrastructure</topic><topic>Plasmopara viticola</topic><topic>Resistance</topic><topic>Sesquiterpenes - metabolism</topic><topic>Species Specificity</topic><topic>Stilbenes</topic><topic>Stilbenes - metabolism</topic><topic>Stomata</topic><topic>Ultrastructure</topic><topic>Vitaceae</topic><topic>Vitis</topic><topic>Vitis - classification</topic><topic>Vitis - metabolism</topic><topic>Vitis - ultrastructure</topic><topic>Vitis vinifera</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alonso-Villaverde, Virginia</creatorcontrib><creatorcontrib>Voinesco, Francine</creatorcontrib><creatorcontrib>Viret, Olivier</creatorcontrib><creatorcontrib>Spring, Jean-Laurent</creatorcontrib><creatorcontrib>Gindro, Katia</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alonso-Villaverde, Virginia</au><au>Voinesco, Francine</au><au>Viret, Olivier</au><au>Spring, Jean-Laurent</au><au>Gindro, Katia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effectiveness of stilbenes in resistant Vitaceae: Ultrastructural and biochemical events during Plasmopara viticola infection process</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>49</volume><issue>3</issue><spage>265</spage><epage>274</epage><pages>265-274</pages><issn>0981-9428</issn><eissn>1873-2690</eissn><coden>PPBIEX</coden><abstract>Leaves of different
Vitis vinifera L. cultivars, susceptible or resistant to downy mildew, Chasselas, Solaris, IRAC 2091 (cvs. Gamaret x Bronner) and
Muscadinia rotundifolia were inoculated with
Plasmopara viticola. Samples were then examined by scanning and transmission electron microscopy, by light microscopy and for their ability to synthesise stilbenes. These phytoalexins were strictly analysed at infection sites. In the susceptible Chasselas,
P. viticola colonises, at 72
h post-infection (hpi), all of the spongy mesophyll with functional haustoria and produces mainly the non toxic piceide. No necrotic zone was observed on Chasselas leaves. The ultrastructural response to downy mildew infection is different in each of the other three resistant grape cultivars. In Solaris, where leaf necrosis are rapidly induced, the infection is restricted to the upper part of the loose spongy mesophyll, and associated with a rapid cell wall disruption and the dispersion of cytoplasmic content along with the production of viniferins. In IRAC 2091, leaf necrosis are quite similar to those observed on Solaris but the infected plant cell, as well as the haustoria, show high electron dense cellular particles without any recognisable organelles, probably related to the effect of the toxic compound pterostilbene, which is synthesised in this grape cultivar. In
M. rotundifolia leaf necrosis are much more scarce and smaller than in other cultivars, but pathogen and plant cells are both strongly affected, with concomitant expulsion of cytoplasmic materials through the stomata after
P. viticola penetration. In this cultivar, the concentration of all identified stilbenes exceeds 1
×
10
3
μmol
mg
−1 FW. The critical role of stilbenes in the resistance of
Vitis spp. is discussed.
► Stilbens synthesis is the most effective way to stop downy mildew development in grapes. ► Resistant vines produce high concentrations of toxic stilbens at the sites of infection. ► Functional haustoria formation is not the key-step for the induction of stilbens synthesis. ► Stilben synthesis can occur in all species of the
Vitaceae family.</abstract><cop>Paris</cop><pub>Elsevier Masson SAS</pub><pmid>21256040</pmid><doi>10.1016/j.plaphy.2010.12.010</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0981-9428 |
| ispartof | Plant physiology and biochemistry, 2011-03, Vol.49 (3), p.265-274 |
| issn | 0981-9428 1873-2690 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_876225199 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Cytoplasm - ultrastructure Downy mildew Fundamental and applied biological sciences. Psychology Fungal plant pathogens Grapevine Leaf Necrosis Oomycetes Phytopathology. Animal pests. Plant and forest protection Plant Diseases - immunology Plant Diseases - microbiology Plant Immunity - physiology Plant Leaves - metabolism Plant Leaves - ultrastructure Plant physiology and development Plant Stomata - ultrastructure Plasmopara viticola Resistance Sesquiterpenes - metabolism Species Specificity Stilbenes Stilbenes - metabolism Stomata Ultrastructure Vitaceae Vitis Vitis - classification Vitis - metabolism Vitis - ultrastructure Vitis vinifera |
| title | The effectiveness of stilbenes in resistant Vitaceae: Ultrastructural and biochemical events during Plasmopara viticola infection process |
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