geminivirus AYVV-derived shuttle vector for tobacco BY2 cells
We have developed a plant-Escherichia coli pASV shuttle vector from the essential elements of the Ageratum yellow vein virus (AYVV). The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassett...
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| Veröffentlicht in: | Plant cell reports 2004-08, Vol.23 (1-2), p.81-90 |
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| creator | Tamilselvi, D Anand, G Swarup, S |
| description | We have developed a plant-Escherichia coli pASV shuttle vector from the essential elements of the Ageratum yellow vein virus (AYVV). The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassettes for plant selection and reporter gene activity. The replication of pASV vectors was compared in Nicotiana benthamiana and N. tabacum BY2 cells, and the latter were found to be suitable for long-term maintenance of the vectors in culture. The vector DNA was detected at regular intervals by PCR, beta-glucuronidase expression analysis and plasmid rescue during a 4-month culture period. A novel methylation-based PCR assay was carried out to show de novo replication for pASV-derived vectors in 2-month-old tobacco BY2 cell lines. This is the first report of the extrachromosomal replication of monopartite begomovirus with stability and foreign gene expression in long-term cell cultures. |
| doi_str_mv | 10.1007/s00299-004-0792-0 |
| format | Article |
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The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassettes for plant selection and reporter gene activity. The replication of pASV vectors was compared in Nicotiana benthamiana and N. tabacum BY2 cells, and the latter were found to be suitable for long-term maintenance of the vectors in culture. The vector DNA was detected at regular intervals by PCR, beta-glucuronidase expression analysis and plasmid rescue during a 4-month culture period. A novel methylation-based PCR assay was carried out to show de novo replication for pASV-derived vectors in 2-month-old tobacco BY2 cell lines. This is the first report of the extrachromosomal replication of monopartite begomovirus with stability and foreign gene expression in long-term cell cultures.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-004-0792-0</identifier><identifier>PMID: 15069580</identifier><language>eng</language><publisher>Germany: Springer Nature B.V</publisher><subject>Ageratum yellow vein virus ; Bacteria ; Begomovirus ; Cell Culture Techniques - methods ; Cells, Cultured - cytology ; Cells, Cultured - metabolism ; Cells, Cultured - virology ; Cloning ; cultured cells ; DNA methylation ; E coli ; electroporation ; Escherichia coli ; Geminiviridae - genetics ; Geminivirus ; gene expression ; Gene Expression Regulation, Plant - genetics ; Gene Expression Regulation, Viral - genetics ; Genes, Reporter - genetics ; Genetic Engineering - methods ; genetic transformation ; genetic vectors ; Genetic Vectors - genetics ; Glucuronidase - genetics ; molecular sequence data ; Nicotiana - genetics ; Nicotiana - growth & development ; Nicotiana - virology ; Nicotiana benthamiana ; Nicotiana tabacum ; nucleotide sequences ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - growth & development ; Plants, Genetically Modified - virology ; Plasmids - genetics ; Polymerase Chain Reaction - methods ; tobacco ; Transformation, Genetic - genetics ; transgenes ; transgenic plants</subject><ispartof>Plant cell reports, 2004-08, Vol.23 (1-2), p.81-90</ispartof><rights>Springer-Verlag 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-38317620f69c21d82ce11ac958f4562cacfc8b08a12e674e131f7abfc32b4f093</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15069580$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tamilselvi, D</creatorcontrib><creatorcontrib>Anand, G</creatorcontrib><creatorcontrib>Swarup, S</creatorcontrib><title>geminivirus AYVV-derived shuttle vector for tobacco BY2 cells</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>We have developed a plant-Escherichia coli pASV shuttle vector from the essential elements of the Ageratum yellow vein virus (AYVV). The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassettes for plant selection and reporter gene activity. The replication of pASV vectors was compared in Nicotiana benthamiana and N. tabacum BY2 cells, and the latter were found to be suitable for long-term maintenance of the vectors in culture. The vector DNA was detected at regular intervals by PCR, beta-glucuronidase expression analysis and plasmid rescue during a 4-month culture period. A novel methylation-based PCR assay was carried out to show de novo replication for pASV-derived vectors in 2-month-old tobacco BY2 cell lines. This is the first report of the extrachromosomal replication of monopartite begomovirus with stability and foreign gene expression in long-term cell cultures.</description><subject>Ageratum yellow vein virus</subject><subject>Bacteria</subject><subject>Begomovirus</subject><subject>Cell Culture Techniques - methods</subject><subject>Cells, Cultured - cytology</subject><subject>Cells, Cultured - metabolism</subject><subject>Cells, Cultured - virology</subject><subject>Cloning</subject><subject>cultured cells</subject><subject>DNA methylation</subject><subject>E coli</subject><subject>electroporation</subject><subject>Escherichia coli</subject><subject>Geminiviridae - genetics</subject><subject>Geminivirus</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Gene Expression Regulation, Viral - genetics</subject><subject>Genes, Reporter - genetics</subject><subject>Genetic Engineering - methods</subject><subject>genetic transformation</subject><subject>genetic vectors</subject><subject>Genetic Vectors - genetics</subject><subject>Glucuronidase - genetics</subject><subject>molecular sequence data</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - growth & development</subject><subject>Nicotiana - virology</subject><subject>Nicotiana benthamiana</subject><subject>Nicotiana tabacum</subject><subject>nucleotide sequences</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - growth & development</subject><subject>Plants, Genetically Modified - virology</subject><subject>Plasmids - genetics</subject><subject>Polymerase Chain Reaction - methods</subject><subject>tobacco</subject><subject>Transformation, Genetic - genetics</subject><subject>transgenes</subject><subject>transgenic plants</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkT1PHDEQhq0oKFxIfgANWaUIlcnM2OuPIgVBISAhURBQqCyvzyaL7m6JvXtS_j0-3UmRUpBiNMU882pGD2OHCCcIoD8XALKWA0gO2hKHV2yGUhAnED9fsxloQq41yn32tpRHgDrU6g3bxxaUbQ3M2JeHuOxX_brPU2lO7-_u-Dzmfh3nTfk1jeMiNusYxiE3qdY4dD6Eofl6T02Ii0V5x_aSX5T4ftcP2O35tx9nF_zq-vvl2ekVDxJx5MII1IogKRsI54ZCRPShXpBkqyj4kILpwHikqLSMKDBp36UgqJMJrDhgx9vcpzz8nmIZ3bIvmwv8Kg5Tcaamk0DCSn56kVRKKwNg_gui1mRRiQp-_Ad8HKa8qu86A7olqbWsEG6hkIdSckzuKfdLn_84BLdx5bauXHXlNq4c1J2jXfDULeP878ZOTgU-bIHkB-cfcl_c7Q0BCgDbEkgrngH-F5Wr</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Tamilselvi, D</creator><creator>Anand, G</creator><creator>Swarup, S</creator><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>geminivirus AYVV-derived shuttle vector for tobacco BY2 cells</title><author>Tamilselvi, D ; Anand, G ; Swarup, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-38317620f69c21d82ce11ac958f4562cacfc8b08a12e674e131f7abfc32b4f093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Ageratum yellow vein virus</topic><topic>Bacteria</topic><topic>Begomovirus</topic><topic>Cell Culture Techniques - methods</topic><topic>Cells, Cultured - cytology</topic><topic>Cells, Cultured - metabolism</topic><topic>Cells, Cultured - virology</topic><topic>Cloning</topic><topic>cultured cells</topic><topic>DNA methylation</topic><topic>E coli</topic><topic>electroporation</topic><topic>Escherichia coli</topic><topic>Geminiviridae - genetics</topic><topic>Geminivirus</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Plant - genetics</topic><topic>Gene Expression Regulation, Viral - genetics</topic><topic>Genes, Reporter - genetics</topic><topic>Genetic Engineering - methods</topic><topic>genetic transformation</topic><topic>genetic vectors</topic><topic>Genetic Vectors - genetics</topic><topic>Glucuronidase - genetics</topic><topic>molecular sequence data</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - growth & development</topic><topic>Nicotiana - virology</topic><topic>Nicotiana benthamiana</topic><topic>Nicotiana tabacum</topic><topic>nucleotide sequences</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - growth & development</topic><topic>Plants, Genetically Modified - virology</topic><topic>Plasmids - genetics</topic><topic>Polymerase Chain Reaction - methods</topic><topic>tobacco</topic><topic>Transformation, Genetic - genetics</topic><topic>transgenes</topic><topic>transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tamilselvi, D</creatorcontrib><creatorcontrib>Anand, G</creatorcontrib><creatorcontrib>Swarup, S</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tamilselvi, D</au><au>Anand, G</au><au>Swarup, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>geminivirus AYVV-derived shuttle vector for tobacco BY2 cells</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2004-08-01</date><risdate>2004</risdate><volume>23</volume><issue>1-2</issue><spage>81</spage><epage>90</epage><pages>81-90</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><abstract>We have developed a plant-Escherichia coli pASV shuttle vector from the essential elements of the Ageratum yellow vein virus (AYVV). The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassettes for plant selection and reporter gene activity. The replication of pASV vectors was compared in Nicotiana benthamiana and N. tabacum BY2 cells, and the latter were found to be suitable for long-term maintenance of the vectors in culture. The vector DNA was detected at regular intervals by PCR, beta-glucuronidase expression analysis and plasmid rescue during a 4-month culture period. A novel methylation-based PCR assay was carried out to show de novo replication for pASV-derived vectors in 2-month-old tobacco BY2 cell lines. This is the first report of the extrachromosomal replication of monopartite begomovirus with stability and foreign gene expression in long-term cell cultures.</abstract><cop>Germany</cop><pub>Springer Nature B.V</pub><pmid>15069580</pmid><doi>10.1007/s00299-004-0792-0</doi><tpages>10</tpages></addata></record> |
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| subjects | Ageratum yellow vein virus Bacteria Begomovirus Cell Culture Techniques - methods Cells, Cultured - cytology Cells, Cultured - metabolism Cells, Cultured - virology Cloning cultured cells DNA methylation E coli electroporation Escherichia coli Geminiviridae - genetics Geminivirus gene expression Gene Expression Regulation, Plant - genetics Gene Expression Regulation, Viral - genetics Genes, Reporter - genetics Genetic Engineering - methods genetic transformation genetic vectors Genetic Vectors - genetics Glucuronidase - genetics molecular sequence data Nicotiana - genetics Nicotiana - growth & development Nicotiana - virology Nicotiana benthamiana Nicotiana tabacum nucleotide sequences Plants, Genetically Modified - genetics Plants, Genetically Modified - growth & development Plants, Genetically Modified - virology Plasmids - genetics Polymerase Chain Reaction - methods tobacco Transformation, Genetic - genetics transgenes transgenic plants |
| title | geminivirus AYVV-derived shuttle vector for tobacco BY2 cells |
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