Feasibility study of corticosteroid treatment for esophageal ulcer after EMR in a canine model
Background Intralesional or systemic steroid administration is a promising strategy for the prevention of esophageal stricture after endoscopic therapy. The aim of this study was to evaluate the influence of steroid therapy on the process of healing of defects in the esophageal mucosa after endoscop...
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| Veröffentlicht in: | Journal of gastroenterology 2011-07, Vol.46 (7), p.866-872 |
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| creator | Honda, Michitaka Nakamura, Tatsuo Hori, Yoshio Shionoya, Yoshiki Yamamoto, Kazumichi Nishizawa, Yuji Kojima, Fumitsugu Shigeno, Keiji |
| description | Background
Intralesional or systemic steroid administration is a promising strategy for the prevention of esophageal stricture after endoscopic therapy. The aim of this study was to evaluate the influence of steroid therapy on the process of healing of defects in the esophageal mucosa after endoscopic mucosal resection (EMR).
Methods
Nine beagle dogs were divided into three equal groups: group A, intralesional injection (
n
= 3), group B, peroral administration (
n
= 3), and group C, untreated control (
n
= 3). In group A, triamcinolone acetonide 1 ml (10 mg) was injected directly into the exposed submucosal layer immediately after EMR, and again on postoperative day (POD) 7. In group B, dogs were administered prednisolone 0.5 mg/kg/day orally for 14 days after EMR. In group C, 1 ml normal saline was injected by the same method as that used for group A. On POD 28, histological examination was performed to evaluate epithelialization, inflammation, angiogenesis, and atrophy of the muscularis propria.
Results
In groups A, B, and C, the mean ulcer area was 50.1, 22.7, and 7.4 mm
2
, respectively. The difference between groups A and C was significant (
p
|
| doi_str_mv | 10.1007/s00535-011-0400-3 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_876190435</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A714586110</galeid><sourcerecordid>A714586110</sourcerecordid><originalsourceid>FETCH-LOGICAL-c556t-7392c27dbc93f03d549e7fc897c16e3dbf5292f5608a4bb0f628ee4af29f0d283</originalsourceid><addsrcrecordid>eNp1kUGL1TAUhYMoznP0B7iRoAtXHW-SpmmWwzCjwoggujWk6c0zQ9s8k3Tx_r15dFQUJYvAzXcON-cQ8pzBBQNQbzKAFLIBxhpoARrxgOxYWydSc_6Q7EC3bcOYas_Ik5zvAJgA2T8mZ5xJrbQQO_L1Bm0OQ5hCOdJc1vFIo6cuphJczAVTDCMtCW2ZcSnUx0Qxx8M3u0c70XVymKj1laPXHz7RsFBLnV3CgnSOI05PySNvp4zP7u9z8uXm-vPVu-b249v3V5e3jZOyK40SmjuuxsFp4UGMstWovOu1cqxDMQ5ecs297KC37TCA73iP2FrPtYeR9-KcvN58Dyl-XzEXM4fscJrsgnHNplcd01CjqeTLv8i7uKalLlchqWoyXFXo1Qbt7YQmLD6WZN3J0lwq1sq-YwwqdfEPqp4R55regj7U-R8Ctglcijkn9OaQwmzT0TAwp0bN1qipjZpTo0ZUzYv7fddhxvGX4meFFeAbkOvTssf0-0P_d_0But6pkw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>875721527</pqid></control><display><type>article</type><title>Feasibility study of corticosteroid treatment for esophageal ulcer after EMR in a canine model</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Honda, Michitaka ; Nakamura, Tatsuo ; Hori, Yoshio ; Shionoya, Yoshiki ; Yamamoto, Kazumichi ; Nishizawa, Yuji ; Kojima, Fumitsugu ; Shigeno, Keiji</creator><creatorcontrib>Honda, Michitaka ; Nakamura, Tatsuo ; Hori, Yoshio ; Shionoya, Yoshiki ; Yamamoto, Kazumichi ; Nishizawa, Yuji ; Kojima, Fumitsugu ; Shigeno, Keiji</creatorcontrib><description>Background
Intralesional or systemic steroid administration is a promising strategy for the prevention of esophageal stricture after endoscopic therapy. The aim of this study was to evaluate the influence of steroid therapy on the process of healing of defects in the esophageal mucosa after endoscopic mucosal resection (EMR).
Methods
Nine beagle dogs were divided into three equal groups: group A, intralesional injection (
n
= 3), group B, peroral administration (
n
= 3), and group C, untreated control (
n
= 3). In group A, triamcinolone acetonide 1 ml (10 mg) was injected directly into the exposed submucosal layer immediately after EMR, and again on postoperative day (POD) 7. In group B, dogs were administered prednisolone 0.5 mg/kg/day orally for 14 days after EMR. In group C, 1 ml normal saline was injected by the same method as that used for group A. On POD 28, histological examination was performed to evaluate epithelialization, inflammation, angiogenesis, and atrophy of the muscularis propria.
Results
In groups A, B, and C, the mean ulcer area was 50.1, 22.7, and 7.4 mm
2
, respectively. The difference between groups A and C was significant (
p
< 0.01). Inflammatory cells were significantly more evident in the lesions of group A than in those of group C (
p
< 0.05). In all groups, atrophy of the muscularis propria was evident. However, transmural destruction and fibrosis were observed only in group A.
Conclusion
It was speculated that the esophageal ulcer causes the fibrosis of the submucosa and atrophy of the muscularis propria during process of healing. Intralesional steroid injection deepened the esophageal ulcers and delayed epithelialization, whereas systemic administration did not clearly improve the lesion healing process.</description><identifier>ISSN: 0944-1174</identifier><identifier>EISSN: 1435-5922</identifier><identifier>DOI: 10.1007/s00535-011-0400-3</identifier><identifier>PMID: 21597933</identifier><language>eng</language><publisher>Japan: Springer Japan</publisher><subject>Abdominal Surgery ; Administration, Oral ; Animals ; Colorectal Surgery ; Corticosteroids ; Disease Models, Animal ; Dogs ; Electronic records ; Endoscopy ; Esophageal Diseases - drug therapy ; Esophageal Diseases - etiology ; Esophageal Stenosis - prevention & control ; Esophagoscopy - adverse effects ; Esophagus - surgery ; Feasibility Studies ; Gastroenterology ; Glucocorticoids - administration & dosage ; Hepatology ; Immunohistochemistry ; Injections ; Medicine ; Medicine & Public Health ; Mucous Membrane - surgery ; Original Article—Alimentary Tract ; Prednisolone ; Prednisolone - administration & dosage ; Surgical Oncology ; Triamcinolone ; Triamcinolone Acetonide - administration & dosage ; Ulcer - drug therapy ; Ulcer - etiology ; Wound Healing - drug effects</subject><ispartof>Journal of gastroenterology, 2011-07, Vol.46 (7), p.866-872</ispartof><rights>Springer 2011</rights><rights>COPYRIGHT 2011 Springer</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-7392c27dbc93f03d549e7fc897c16e3dbf5292f5608a4bb0f628ee4af29f0d283</citedby><cites>FETCH-LOGICAL-c556t-7392c27dbc93f03d549e7fc897c16e3dbf5292f5608a4bb0f628ee4af29f0d283</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00535-011-0400-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00535-011-0400-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21597933$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Honda, Michitaka</creatorcontrib><creatorcontrib>Nakamura, Tatsuo</creatorcontrib><creatorcontrib>Hori, Yoshio</creatorcontrib><creatorcontrib>Shionoya, Yoshiki</creatorcontrib><creatorcontrib>Yamamoto, Kazumichi</creatorcontrib><creatorcontrib>Nishizawa, Yuji</creatorcontrib><creatorcontrib>Kojima, Fumitsugu</creatorcontrib><creatorcontrib>Shigeno, Keiji</creatorcontrib><title>Feasibility study of corticosteroid treatment for esophageal ulcer after EMR in a canine model</title><title>Journal of gastroenterology</title><addtitle>J Gastroenterol</addtitle><addtitle>J Gastroenterol</addtitle><description>Background
Intralesional or systemic steroid administration is a promising strategy for the prevention of esophageal stricture after endoscopic therapy. The aim of this study was to evaluate the influence of steroid therapy on the process of healing of defects in the esophageal mucosa after endoscopic mucosal resection (EMR).
Methods
Nine beagle dogs were divided into three equal groups: group A, intralesional injection (
n
= 3), group B, peroral administration (
n
= 3), and group C, untreated control (
n
= 3). In group A, triamcinolone acetonide 1 ml (10 mg) was injected directly into the exposed submucosal layer immediately after EMR, and again on postoperative day (POD) 7. In group B, dogs were administered prednisolone 0.5 mg/kg/day orally for 14 days after EMR. In group C, 1 ml normal saline was injected by the same method as that used for group A. On POD 28, histological examination was performed to evaluate epithelialization, inflammation, angiogenesis, and atrophy of the muscularis propria.
Results
In groups A, B, and C, the mean ulcer area was 50.1, 22.7, and 7.4 mm
2
, respectively. The difference between groups A and C was significant (
p
< 0.01). Inflammatory cells were significantly more evident in the lesions of group A than in those of group C (
p
< 0.05). In all groups, atrophy of the muscularis propria was evident. However, transmural destruction and fibrosis were observed only in group A.
Conclusion
It was speculated that the esophageal ulcer causes the fibrosis of the submucosa and atrophy of the muscularis propria during process of healing. Intralesional steroid injection deepened the esophageal ulcers and delayed epithelialization, whereas systemic administration did not clearly improve the lesion healing process.</description><subject>Abdominal Surgery</subject><subject>Administration, Oral</subject><subject>Animals</subject><subject>Colorectal Surgery</subject><subject>Corticosteroids</subject><subject>Disease Models, Animal</subject><subject>Dogs</subject><subject>Electronic records</subject><subject>Endoscopy</subject><subject>Esophageal Diseases - drug therapy</subject><subject>Esophageal Diseases - etiology</subject><subject>Esophageal Stenosis - prevention & control</subject><subject>Esophagoscopy - adverse effects</subject><subject>Esophagus - surgery</subject><subject>Feasibility Studies</subject><subject>Gastroenterology</subject><subject>Glucocorticoids - administration & dosage</subject><subject>Hepatology</subject><subject>Immunohistochemistry</subject><subject>Injections</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Mucous Membrane - surgery</subject><subject>Original Article—Alimentary Tract</subject><subject>Prednisolone</subject><subject>Prednisolone - administration & dosage</subject><subject>Surgical Oncology</subject><subject>Triamcinolone</subject><subject>Triamcinolone Acetonide - administration & dosage</subject><subject>Ulcer - drug therapy</subject><subject>Ulcer - etiology</subject><subject>Wound Healing - drug effects</subject><issn>0944-1174</issn><issn>1435-5922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp1kUGL1TAUhYMoznP0B7iRoAtXHW-SpmmWwzCjwoggujWk6c0zQ9s8k3Tx_r15dFQUJYvAzXcON-cQ8pzBBQNQbzKAFLIBxhpoARrxgOxYWydSc_6Q7EC3bcOYas_Ik5zvAJgA2T8mZ5xJrbQQO_L1Bm0OQ5hCOdJc1vFIo6cuphJczAVTDCMtCW2ZcSnUx0Qxx8M3u0c70XVymKj1laPXHz7RsFBLnV3CgnSOI05PySNvp4zP7u9z8uXm-vPVu-b249v3V5e3jZOyK40SmjuuxsFp4UGMstWovOu1cqxDMQ5ecs297KC37TCA73iP2FrPtYeR9-KcvN58Dyl-XzEXM4fscJrsgnHNplcd01CjqeTLv8i7uKalLlchqWoyXFXo1Qbt7YQmLD6WZN3J0lwq1sq-YwwqdfEPqp4R55regj7U-R8Ctglcijkn9OaQwmzT0TAwp0bN1qipjZpTo0ZUzYv7fddhxvGX4meFFeAbkOvTssf0-0P_d_0But6pkw</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Honda, Michitaka</creator><creator>Nakamura, Tatsuo</creator><creator>Hori, Yoshio</creator><creator>Shionoya, Yoshiki</creator><creator>Yamamoto, Kazumichi</creator><creator>Nishizawa, Yuji</creator><creator>Kojima, Fumitsugu</creator><creator>Shigeno, Keiji</creator><general>Springer Japan</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20110701</creationdate><title>Feasibility study of corticosteroid treatment for esophageal ulcer after EMR in a canine model</title><author>Honda, Michitaka ; Nakamura, Tatsuo ; Hori, Yoshio ; Shionoya, Yoshiki ; Yamamoto, Kazumichi ; Nishizawa, Yuji ; Kojima, Fumitsugu ; Shigeno, Keiji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-7392c27dbc93f03d549e7fc897c16e3dbf5292f5608a4bb0f628ee4af29f0d283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Abdominal Surgery</topic><topic>Administration, Oral</topic><topic>Animals</topic><topic>Colorectal Surgery</topic><topic>Corticosteroids</topic><topic>Disease Models, Animal</topic><topic>Dogs</topic><topic>Electronic records</topic><topic>Endoscopy</topic><topic>Esophageal Diseases - drug therapy</topic><topic>Esophageal Diseases - etiology</topic><topic>Esophageal Stenosis - prevention & control</topic><topic>Esophagoscopy - adverse effects</topic><topic>Esophagus - surgery</topic><topic>Feasibility Studies</topic><topic>Gastroenterology</topic><topic>Glucocorticoids - administration & dosage</topic><topic>Hepatology</topic><topic>Immunohistochemistry</topic><topic>Injections</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Mucous Membrane - surgery</topic><topic>Original Article—Alimentary Tract</topic><topic>Prednisolone</topic><topic>Prednisolone - administration & dosage</topic><topic>Surgical Oncology</topic><topic>Triamcinolone</topic><topic>Triamcinolone Acetonide - administration & dosage</topic><topic>Ulcer - drug therapy</topic><topic>Ulcer - etiology</topic><topic>Wound Healing - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Honda, Michitaka</creatorcontrib><creatorcontrib>Nakamura, Tatsuo</creatorcontrib><creatorcontrib>Hori, Yoshio</creatorcontrib><creatorcontrib>Shionoya, Yoshiki</creatorcontrib><creatorcontrib>Yamamoto, Kazumichi</creatorcontrib><creatorcontrib>Nishizawa, Yuji</creatorcontrib><creatorcontrib>Kojima, Fumitsugu</creatorcontrib><creatorcontrib>Shigeno, Keiji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of gastroenterology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Honda, Michitaka</au><au>Nakamura, Tatsuo</au><au>Hori, Yoshio</au><au>Shionoya, Yoshiki</au><au>Yamamoto, Kazumichi</au><au>Nishizawa, Yuji</au><au>Kojima, Fumitsugu</au><au>Shigeno, Keiji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Feasibility study of corticosteroid treatment for esophageal ulcer after EMR in a canine model</atitle><jtitle>Journal of gastroenterology</jtitle><stitle>J Gastroenterol</stitle><addtitle>J Gastroenterol</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>46</volume><issue>7</issue><spage>866</spage><epage>872</epage><pages>866-872</pages><issn>0944-1174</issn><eissn>1435-5922</eissn><abstract>Background
Intralesional or systemic steroid administration is a promising strategy for the prevention of esophageal stricture after endoscopic therapy. The aim of this study was to evaluate the influence of steroid therapy on the process of healing of defects in the esophageal mucosa after endoscopic mucosal resection (EMR).
Methods
Nine beagle dogs were divided into three equal groups: group A, intralesional injection (
n
= 3), group B, peroral administration (
n
= 3), and group C, untreated control (
n
= 3). In group A, triamcinolone acetonide 1 ml (10 mg) was injected directly into the exposed submucosal layer immediately after EMR, and again on postoperative day (POD) 7. In group B, dogs were administered prednisolone 0.5 mg/kg/day orally for 14 days after EMR. In group C, 1 ml normal saline was injected by the same method as that used for group A. On POD 28, histological examination was performed to evaluate epithelialization, inflammation, angiogenesis, and atrophy of the muscularis propria.
Results
In groups A, B, and C, the mean ulcer area was 50.1, 22.7, and 7.4 mm
2
, respectively. The difference between groups A and C was significant (
p
< 0.01). Inflammatory cells were significantly more evident in the lesions of group A than in those of group C (
p
< 0.05). In all groups, atrophy of the muscularis propria was evident. However, transmural destruction and fibrosis were observed only in group A.
Conclusion
It was speculated that the esophageal ulcer causes the fibrosis of the submucosa and atrophy of the muscularis propria during process of healing. Intralesional steroid injection deepened the esophageal ulcers and delayed epithelialization, whereas systemic administration did not clearly improve the lesion healing process.</abstract><cop>Japan</cop><pub>Springer Japan</pub><pmid>21597933</pmid><doi>10.1007/s00535-011-0400-3</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0944-1174 |
| ispartof | Journal of gastroenterology, 2011-07, Vol.46 (7), p.866-872 |
| issn | 0944-1174 1435-5922 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_876190435 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Abdominal Surgery Administration, Oral Animals Colorectal Surgery Corticosteroids Disease Models, Animal Dogs Electronic records Endoscopy Esophageal Diseases - drug therapy Esophageal Diseases - etiology Esophageal Stenosis - prevention & control Esophagoscopy - adverse effects Esophagus - surgery Feasibility Studies Gastroenterology Glucocorticoids - administration & dosage Hepatology Immunohistochemistry Injections Medicine Medicine & Public Health Mucous Membrane - surgery Original Article—Alimentary Tract Prednisolone Prednisolone - administration & dosage Surgical Oncology Triamcinolone Triamcinolone Acetonide - administration & dosage Ulcer - drug therapy Ulcer - etiology Wound Healing - drug effects |
| title | Feasibility study of corticosteroid treatment for esophageal ulcer after EMR in a canine model |
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