Cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete Phanerochaete chrysosporium

Anion exchangers are membrane proteins that have been identified in a wide variety of species, where they transport Cl − and HCO 3 - across the cell membrane. In this study, we cloned an anion-exchange protein from the genome of the basidiomycete Phanerochaete chrysosporium (PcAEP). PcAEP is a 618-a...

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Veröffentlicht in:	Protein expression and purification 2011-09, Vol.79 (1), p.81-87
Hauptverfasser:	Tokuda, Natsuko, Igarashi, Kiyohiko, Shimamura, Tatsuro, Yurugi-Kobayashi, Takami, Shiroishi, Mitsunori, Ito, Keisuke, Sugawara, Taishi, Asada, Hidetsugu, Murata, Takeshi, Nomura, Norimichi, Iwata, So, Kobayashi, Takuya
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Anion Exchange Protein 1, Erythrocyte - genetics Anion exchanger Antiporters - analysis Antiporters - genetics Antiporters - isolation & purification Cell Membrane - ultrastructure Cloning Cloning, Molecular DNA, Complementary - genetics Electrophoresis, Polyacrylamide Gel Fungal Proteins - analysis Fungal Proteins - genetics Fungal Proteins - isolation & purification Humans Molecular Sequence Data Phanerochaete - genetics Phanerochaete chrysosporium Pichia - genetics Pichia pastoris Purification Sequence Alignment Solubility Up-Regulation
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