Quantitative Analysis of Thymidine Kinase 1 and 5'(3')-Deoxyribonucleotidase mRNA Expression: The Role of Fluorothymidine Uptake

Thymidine kinase 1 (TK1) and 5'(3')-deoxyribonucleotidase (dNT1) regulate the metabolism of thymidine and its analogs. However, the expression patterns and roles of these enzymes in fluorothymidine (FLT) uptake have not been systemically studied. TK1 and dNT1 mRNAs were determined by quant...

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Veröffentlicht in:	Anticancer research 2011-06, Vol.31 (6), p.2135-2139
Hauptverfasser:	Lee, Seung Jin, Lee, Haeng Jung, Moon, Dae Hyuk
Format:	Artikel
Sprache:	eng
Schlagworte:	5'-Nucleotidase - biosynthesis 5'-Nucleotidase - genetics Biological and medical sciences Cell Line, Tumor Colonic Neoplasms - enzymology Colonic Neoplasms - genetics Colonic Neoplasms - metabolism Dideoxynucleosides - pharmacokinetics HCT116 Cells HT29 Cells Humans Lung Neoplasms - enzymology Lung Neoplasms - genetics Lung Neoplasms - metabolism Medical sciences Pneumology RNA, Messenger - biosynthesis RNA, Messenger - genetics Thymidine Kinase - biosynthesis Thymidine Kinase - genetics Tritium Tumors Tumors of the respiratory system and mediastinum
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Zusammenfassung:	Thymidine kinase 1 (TK1) and 5'(3')-deoxyribonucleotidase (dNT1) regulate the metabolism of thymidine and its analogs. However, the expression patterns and roles of these enzymes in fluorothymidine (FLT) uptake have not been systemically studied. TK1 and dNT1 mRNAs were determined by quantitative PCR in 20 asynchronously growing lung and colon cancer cell lines. [3H]FLT uptake and doubling time were measured. The TK1/GAPDH values varied from 3.09×10(-3)±6.62×10(-4) to 2.44×10(-2)±8.49×10(-4) and dNT1/GAPDH values from 5.84×10(-4)±4.88×10(-5) to 1.59×10(-2)±1.20×10(-3). The correlation coefficient of TK1/GAPDH versus dNT1/GAPDH was 0.669 (p
ISSN:	0250-7005 1791-7530