Canine sperm vitrification with sucrose: effect on sperm function
Summary The ability of sucrose to protect spermatozoa against mitochondrial damage, artificial acrosome reaction and DNA fragmentation during ultra‐rapid cryopreservation in canine sperm was investigated. Swim‐up selected spermatozoa of second‐fraction semen were vitrified with different concentrati...
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| Veröffentlicht in: | Andrologia 2011-08, Vol.43 (4), p.233-241 |
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| creator | Sánchez, R. Risopatrón, J. Schulz, M. Villegas, J. Isachenko, V. Kreinberg, R. Isachenko, E. |
| description | Summary
The ability of sucrose to protect spermatozoa against mitochondrial damage, artificial acrosome reaction and DNA fragmentation during ultra‐rapid cryopreservation in canine sperm was investigated. Swim‐up selected spermatozoa of second‐fraction semen were vitrified with different concentrations of sucrose (0.1, 0.25 and 0.4 m) in proportion 1 : 1 v/v with HTF–BSA 1%. From each group, 30‐μl suspensions of cells were dropped directly into liquid nitrogen and stored for at least 24 h. Cells were thawed by submerging the spheres in HTF with 1% BSA at 37 °C. The number of progressively motile spermatozoa was significantly higher in the sucrose 0.25 m + HTF–BSA 1% (42.5 ± 2.3%, P |
| doi_str_mv | 10.1111/j.1439-0272.2010.01054.x |
| format | Article |
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The ability of sucrose to protect spermatozoa against mitochondrial damage, artificial acrosome reaction and DNA fragmentation during ultra‐rapid cryopreservation in canine sperm was investigated. Swim‐up selected spermatozoa of second‐fraction semen were vitrified with different concentrations of sucrose (0.1, 0.25 and 0.4 m) in proportion 1 : 1 v/v with HTF–BSA 1%. From each group, 30‐μl suspensions of cells were dropped directly into liquid nitrogen and stored for at least 24 h. Cells were thawed by submerging the spheres in HTF with 1% BSA at 37 °C. The number of progressively motile spermatozoa was significantly higher in the sucrose 0.25 m + HTF–BSA 1% (42.5 ± 2.3%, P < 0.01) than in HTF only (1.66 ± 0.3%). The same combination of sucrose 0.25 m + HTF–BSA 1% (42.7 ± 1.5%) had a stronger cryoprotective effect on the integrity of mitochondrial membrane potential (P < 0.05) and decreased the DNA fragmentation (2.8 ± 0.5%) as compared with HTF only (1.93 ± 0.6% and 5.6 ± 0.6% respectively). With respect to acrosome‐reacted spermatozoa, no significant difference was found between the groups investigated (P > 0.05). It is concluded that sucrose, a nonpermeable cryoprotectant, can effectively preserve important physiological parameters such as mitochondrial membrane potential and DNA integrity during ultra‐rapid cryopreservation.</description><identifier>ISSN: 0303-4569</identifier><identifier>EISSN: 1439-0272</identifier><identifier>DOI: 10.1111/j.1439-0272.2010.01054.x</identifier><identifier>PMID: 21486402</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Canine sperm ; Cryopreservation ; Cryoprotective Agents ; DNA Fragmentation - drug effects ; Dogs ; Male ; Membrane Potential, Mitochondrial - drug effects ; Semen Preservation - methods ; sperm function ; Spermatozoa - drug effects ; Spermatozoa - physiology ; sucrose ; Sucrose - pharmacology ; Vitrification</subject><ispartof>Andrologia, 2011-08, Vol.43 (4), p.233-241</ispartof><rights>2011 Blackwell Verlag GmbH</rights><rights>2011 Blackwell Verlag GmbH.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4074-f75c3c09aba4ae569f651b5069ff9392d2e3a6612b7dd2e00ddc14892992e1bf3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0272.2010.01054.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0272.2010.01054.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21486402$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sánchez, R.</creatorcontrib><creatorcontrib>Risopatrón, J.</creatorcontrib><creatorcontrib>Schulz, M.</creatorcontrib><creatorcontrib>Villegas, J.</creatorcontrib><creatorcontrib>Isachenko, V.</creatorcontrib><creatorcontrib>Kreinberg, R.</creatorcontrib><creatorcontrib>Isachenko, E.</creatorcontrib><title>Canine sperm vitrification with sucrose: effect on sperm function</title><title>Andrologia</title><addtitle>Andrologia</addtitle><description>Summary
The ability of sucrose to protect spermatozoa against mitochondrial damage, artificial acrosome reaction and DNA fragmentation during ultra‐rapid cryopreservation in canine sperm was investigated. Swim‐up selected spermatozoa of second‐fraction semen were vitrified with different concentrations of sucrose (0.1, 0.25 and 0.4 m) in proportion 1 : 1 v/v with HTF–BSA 1%. From each group, 30‐μl suspensions of cells were dropped directly into liquid nitrogen and stored for at least 24 h. Cells were thawed by submerging the spheres in HTF with 1% BSA at 37 °C. The number of progressively motile spermatozoa was significantly higher in the sucrose 0.25 m + HTF–BSA 1% (42.5 ± 2.3%, P < 0.01) than in HTF only (1.66 ± 0.3%). The same combination of sucrose 0.25 m + HTF–BSA 1% (42.7 ± 1.5%) had a stronger cryoprotective effect on the integrity of mitochondrial membrane potential (P < 0.05) and decreased the DNA fragmentation (2.8 ± 0.5%) as compared with HTF only (1.93 ± 0.6% and 5.6 ± 0.6% respectively). With respect to acrosome‐reacted spermatozoa, no significant difference was found between the groups investigated (P > 0.05). It is concluded that sucrose, a nonpermeable cryoprotectant, can effectively preserve important physiological parameters such as mitochondrial membrane potential and DNA integrity during ultra‐rapid cryopreservation.</description><subject>Animals</subject><subject>Canine sperm</subject><subject>Cryopreservation</subject><subject>Cryoprotective Agents</subject><subject>DNA Fragmentation - drug effects</subject><subject>Dogs</subject><subject>Male</subject><subject>Membrane Potential, Mitochondrial - drug effects</subject><subject>Semen Preservation - methods</subject><subject>sperm function</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>sucrose</subject><subject>Sucrose - pharmacology</subject><subject>Vitrification</subject><issn>0303-4569</issn><issn>1439-0272</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOwzAQRS0EolXpL6DsWKX4lbhGYlEFKKilbHgsLcexhUseJU5o-_c4pHQky1cz51rjC0CA4AT5ul5PECU8hJjhCYa-609EJ7sTMDwOTsEQEkhCGsV8AMbOraEvGjFG6TkYYESnMYV4CGaJLG2pA7fRdRH82Ka2xirZ2KoMtrb5DFyr6srpm0Abo1UT-H7PmrZUHXYBzozMnR4f7hF4e7h_TR7D5cv8KZktQ0Uho6FhkSIKcplKKrVfy8QRSiPoheGE4wxrIuMY4ZRlXkOYZcpvyTHnWKPUkBG46t_d1NV3q10jCuuUznNZ6qp1YsoiyhGZYk9eHsg2LXQmNrUtZL0X_7_2wG0PbG2u98c5gqILWKxFl6PochRdwOIvYLETs9Vdp7w_7P3WNXp39Mv6S8SMsEh8rOZiQRfvCX--E3PyC8mifJU</recordid><startdate>201108</startdate><enddate>201108</enddate><creator>Sánchez, R.</creator><creator>Risopatrón, J.</creator><creator>Schulz, M.</creator><creator>Villegas, J.</creator><creator>Isachenko, V.</creator><creator>Kreinberg, R.</creator><creator>Isachenko, E.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201108</creationdate><title>Canine sperm vitrification with sucrose: effect on sperm function</title><author>Sánchez, R. ; Risopatrón, J. ; Schulz, M. ; Villegas, J. ; Isachenko, V. ; Kreinberg, R. ; Isachenko, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4074-f75c3c09aba4ae569f651b5069ff9392d2e3a6612b7dd2e00ddc14892992e1bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Canine sperm</topic><topic>Cryopreservation</topic><topic>Cryoprotective Agents</topic><topic>DNA Fragmentation - drug effects</topic><topic>Dogs</topic><topic>Male</topic><topic>Membrane Potential, Mitochondrial - drug effects</topic><topic>Semen Preservation - methods</topic><topic>sperm function</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>sucrose</topic><topic>Sucrose - pharmacology</topic><topic>Vitrification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sánchez, R.</creatorcontrib><creatorcontrib>Risopatrón, J.</creatorcontrib><creatorcontrib>Schulz, M.</creatorcontrib><creatorcontrib>Villegas, J.</creatorcontrib><creatorcontrib>Isachenko, V.</creatorcontrib><creatorcontrib>Kreinberg, R.</creatorcontrib><creatorcontrib>Isachenko, E.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Andrologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sánchez, R.</au><au>Risopatrón, J.</au><au>Schulz, M.</au><au>Villegas, J.</au><au>Isachenko, V.</au><au>Kreinberg, R.</au><au>Isachenko, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Canine sperm vitrification with sucrose: effect on sperm function</atitle><jtitle>Andrologia</jtitle><addtitle>Andrologia</addtitle><date>2011-08</date><risdate>2011</risdate><volume>43</volume><issue>4</issue><spage>233</spage><epage>241</epage><pages>233-241</pages><issn>0303-4569</issn><eissn>1439-0272</eissn><abstract>Summary
The ability of sucrose to protect spermatozoa against mitochondrial damage, artificial acrosome reaction and DNA fragmentation during ultra‐rapid cryopreservation in canine sperm was investigated. Swim‐up selected spermatozoa of second‐fraction semen were vitrified with different concentrations of sucrose (0.1, 0.25 and 0.4 m) in proportion 1 : 1 v/v with HTF–BSA 1%. From each group, 30‐μl suspensions of cells were dropped directly into liquid nitrogen and stored for at least 24 h. Cells were thawed by submerging the spheres in HTF with 1% BSA at 37 °C. The number of progressively motile spermatozoa was significantly higher in the sucrose 0.25 m + HTF–BSA 1% (42.5 ± 2.3%, P < 0.01) than in HTF only (1.66 ± 0.3%). The same combination of sucrose 0.25 m + HTF–BSA 1% (42.7 ± 1.5%) had a stronger cryoprotective effect on the integrity of mitochondrial membrane potential (P < 0.05) and decreased the DNA fragmentation (2.8 ± 0.5%) as compared with HTF only (1.93 ± 0.6% and 5.6 ± 0.6% respectively). With respect to acrosome‐reacted spermatozoa, no significant difference was found between the groups investigated (P > 0.05). It is concluded that sucrose, a nonpermeable cryoprotectant, can effectively preserve important physiological parameters such as mitochondrial membrane potential and DNA integrity during ultra‐rapid cryopreservation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21486402</pmid><doi>10.1111/j.1439-0272.2010.01054.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Canine sperm Cryopreservation Cryoprotective Agents DNA Fragmentation - drug effects Dogs Male Membrane Potential, Mitochondrial - drug effects Semen Preservation - methods sperm function Spermatozoa - drug effects Spermatozoa - physiology sucrose Sucrose - pharmacology Vitrification |
| title | Canine sperm vitrification with sucrose: effect on sperm function |
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