CD47 regulates the TGF-β signaling pathway in osteoblasts and is distributed in Meckel's cartilage
Previously, CD47 gene expression has been shown to increase during mandible development using a micro array technique. To determine the function of CD47 in osteoblasts, CD47 was silenced using siRNA in vitro. The TGF-β1 and phosphorylated-Smad2 levels and transcription factor genes related to bone m...
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| Veröffentlicht in: | Journal of Oral Science 2011, Vol.53(2), pp.169-175 |
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| container_title | Journal of Oral Science |
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| creator | Shimada, Koichi Nakajima, Akira Ikeda, Kyoko Ishibashi, Kazuya Shimizu, Noriyoshi Ito, Koichi |
| description | Previously, CD47 gene expression has been shown to increase during mandible development using a micro array technique. To determine the function of CD47 in osteoblasts, CD47 was silenced using siRNA in vitro. The TGF-β1 and phosphorylated-Smad2 levels and transcription factor genes related to bone metabolism increased dose-dependently with CD47 silencing. Furthermore, we determined the distribution of CD47 in mouse embryonic E13 and E15 in vivo. The CD47-positive cells were localized in Meckel's cartilage and antenatal mandibular bone. These results suggest that TGF-β1 signaling and mandible development might be regulated by CD47. (J Oral Sci 53, 169-175, 2011) |
| doi_str_mv | 10.2334/josnusd.53.169 |
| format | Article |
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To determine the function of CD47 in osteoblasts, CD47 was silenced using siRNA in vitro. The TGF-β1 and phosphorylated-Smad2 levels and transcription factor genes related to bone metabolism increased dose-dependently with CD47 silencing. Furthermore, we determined the distribution of CD47 in mouse embryonic E13 and E15 in vivo. The CD47-positive cells were localized in Meckel's cartilage and antenatal mandibular bone. These results suggest that TGF-β1 signaling and mandible development might be regulated by CD47. 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To determine the function of CD47 in osteoblasts, CD47 was silenced using siRNA in vitro. The TGF-β1 and phosphorylated-Smad2 levels and transcription factor genes related to bone metabolism increased dose-dependently with CD47 silencing. Furthermore, we determined the distribution of CD47 in mouse embryonic E13 and E15 in vivo. The CD47-positive cells were localized in Meckel's cartilage and antenatal mandibular bone. These results suggest that TGF-β1 signaling and mandible development might be regulated by CD47. (J Oral Sci 53, 169-175, 2011)</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cartilage - embryology</subject><subject>CD47</subject><subject>CD47 Antigen - analysis</subject><subject>CD47 Antigen - genetics</subject><subject>CD47 Antigen - physiology</subject><subject>Cell Culture Techniques</subject><subject>Dentistry</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Gene Silencing - physiology</subject><subject>Gestational Age</subject><subject>Immunohistochemistry</subject><subject>Mandible - embryology</subject><subject>Mandible - metabolism</subject><subject>mandibular development</subject><subject>Meckel's cartilage</subject><subject>Mesoderm - physiology</subject><subject>Mice</subject><subject>osteoblasts</subject><subject>Osteoblasts - physiology</subject><subject>Polymerase Chain Reaction</subject><subject>RNA, Small Interfering - genetics</subject><subject>Signal Transduction - genetics</subject><subject>Signal Transduction - physiology</subject><subject>Smad2 Protein - analysis</subject><subject>Smad2 Protein - genetics</subject><subject>TGF-β</subject><subject>Transcription Factors - genetics</subject><subject>Transforming Growth Factor beta1 - analysis</subject><subject>Transforming Growth Factor beta1 - genetics</subject><subject>Transforming Growth Factor beta1 - physiology</subject><issn>1343-4934</issn><issn>1880-4926</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkMtu2zAQRYkiRZO63XYZcJeVXL5EUcvAeRVI0U26JihybNOlJYdDIchv9UP6TZFhx-hmZoA5c4E5hHzjbC6kVN83A_Yjhnkt51y3H8gFN4ZVqhX6bJqlktMs1Tn5jLhhTAnd1J_IueANF1rwC-IXN6qhGVZjcgWQljXQp_u76t9finHVuxT7Fd25sn5xrzT2dMACQ5ccFqSuDzQiDRFLjt1YIOyJn-D_QLpC6l0uMbkVfCEfly4hfD32Gfl9d_u0eKgef93_WFw_Vr5mrK28MVosTeB160LwojNKh9azWhsNfumY6XgDrNPMeC-CM741sgEtTVurmoGckatD7i4PzyNgsduIHlJyPQwjWtMo0Wo9SZmR-YH0eUDMsLS7HLcuv1rO7N6rPXq1tbST1-ng8hg9dlsIJ_xd5AQsDsAGy_TyCdg78An-zxOHMsWetn7tsoVevgHaGo64</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Shimada, Koichi</creator><creator>Nakajima, Akira</creator><creator>Ikeda, Kyoko</creator><creator>Ishibashi, Kazuya</creator><creator>Shimizu, Noriyoshi</creator><creator>Ito, Koichi</creator><general>Nihon University School of Dentistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2011</creationdate><title>CD47 regulates the TGF-β signaling pathway in osteoblasts and is distributed in Meckel's cartilage</title><author>Shimada, Koichi ; Nakajima, Akira ; Ikeda, Kyoko ; Ishibashi, Kazuya ; Shimizu, Noriyoshi ; Ito, Koichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5009-c8862f8d159addc2b846d9c05686ecfa08b17e0b608cc2da8c9837e63895450e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>3T3 Cells</topic><topic>Animals</topic><topic>Blotting, Western</topic><topic>Cartilage - embryology</topic><topic>CD47</topic><topic>CD47 Antigen - analysis</topic><topic>CD47 Antigen - genetics</topic><topic>CD47 Antigen - physiology</topic><topic>Cell Culture Techniques</topic><topic>Dentistry</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Gene Silencing - physiology</topic><topic>Gestational Age</topic><topic>Immunohistochemistry</topic><topic>Mandible - embryology</topic><topic>Mandible - metabolism</topic><topic>mandibular development</topic><topic>Meckel's cartilage</topic><topic>Mesoderm - physiology</topic><topic>Mice</topic><topic>osteoblasts</topic><topic>Osteoblasts - physiology</topic><topic>Polymerase Chain Reaction</topic><topic>RNA, Small Interfering - genetics</topic><topic>Signal Transduction - genetics</topic><topic>Signal Transduction - physiology</topic><topic>Smad2 Protein - analysis</topic><topic>Smad2 Protein - genetics</topic><topic>TGF-β</topic><topic>Transcription Factors - genetics</topic><topic>Transforming Growth Factor beta1 - analysis</topic><topic>Transforming Growth Factor beta1 - genetics</topic><topic>Transforming Growth Factor beta1 - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shimada, Koichi</creatorcontrib><creatorcontrib>Nakajima, Akira</creatorcontrib><creatorcontrib>Ikeda, Kyoko</creatorcontrib><creatorcontrib>Ishibashi, Kazuya</creatorcontrib><creatorcontrib>Shimizu, Noriyoshi</creatorcontrib><creatorcontrib>Ito, Koichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Oral Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimada, Koichi</au><au>Nakajima, Akira</au><au>Ikeda, Kyoko</au><au>Ishibashi, Kazuya</au><au>Shimizu, Noriyoshi</au><au>Ito, Koichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CD47 regulates the TGF-β signaling pathway in osteoblasts and is distributed in Meckel's cartilage</atitle><jtitle>Journal of Oral Science</jtitle><addtitle>J Oral Sci</addtitle><date>2011</date><risdate>2011</risdate><volume>53</volume><issue>2</issue><spage>169</spage><epage>175</epage><pages>169-175</pages><issn>1343-4934</issn><eissn>1880-4926</eissn><abstract>Previously, CD47 gene expression has been shown to increase during mandible development using a micro array technique. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan |
| subjects | 3T3 Cells Animals Blotting, Western Cartilage - embryology CD47 CD47 Antigen - analysis CD47 Antigen - genetics CD47 Antigen - physiology Cell Culture Techniques Dentistry Electrophoresis, Polyacrylamide Gel Gene Silencing - physiology Gestational Age Immunohistochemistry Mandible - embryology Mandible - metabolism mandibular development Meckel's cartilage Mesoderm - physiology Mice osteoblasts Osteoblasts - physiology Polymerase Chain Reaction RNA, Small Interfering - genetics Signal Transduction - genetics Signal Transduction - physiology Smad2 Protein - analysis Smad2 Protein - genetics TGF-β Transcription Factors - genetics Transforming Growth Factor beta1 - analysis Transforming Growth Factor beta1 - genetics Transforming Growth Factor beta1 - physiology |
| title | CD47 regulates the TGF-β signaling pathway in osteoblasts and is distributed in Meckel's cartilage |
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