Association of T cells and eosinophils with Staphylococcus aureus exotoxin A and toxic shock syndrome toxin 1 in nasal polyps
Staphylococcus aureus exotoxins (SEs) have been implicated in the pathogenesis of nasal polyps (NPs) in patients with chronic rhinosinusitis (CRS). The aim of this study was to identify the correlation between SEs and T cells in NPs. After a nasal smear culture, the nasal lavage fluid and NP tissue...
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| Veröffentlicht in: | American journal of rhinology & allergy 2011-01, Vol.25 (1), p.19-24 |
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| creator | Kim, Seon Tae Chung, Seung Won Jung, Joo Hyun Ha, Jong Su Kang, Il Gyu |
| description | Staphylococcus aureus exotoxins (SEs) have been implicated in the pathogenesis of nasal polyps (NPs) in patients with chronic rhinosinusitis (CRS). The aim of this study was to identify the correlation between SEs and T cells in NPs.
After a nasal smear culture, the nasal lavage fluid and NP tissue were obtained from 30 CRS with NP (CRSwNP) patients. The control samples were taken from 10 normal individuals. The presence of S. aureus exotoxin A (SEA) and toxic shock syndrome toxin 1 (TSST-1) was verified through a polymerase chain reaction (PCR) analysis and immunohistochemical staining. Immunohistochemical staining for CD4(+) and CD8(+) T cells was performed, followed by hematoxylin and eosin (H&E) staining for eosinophils.
The PCR analysis and immunohistochemical staining for SEA and TSST-1 showed a significantly higher expression in the NP group when compared with that in the control group. Furthermore, the number of T cells and eosinophils were notably increased in the NP group when compared with that in the control group. Significant correlation was observed between the results of the culture, PCR, and immunohistochemical staining in the NP group. Moreover, the number of T cells and eosinophils was significantly increased in the positive experimental groups, when compared with that in the control group.
Our results indicate that there was, indeed, some correlation between the expression of SE in the nasal cavity and the increased number of T cells and eosinophils in NPs. |
| doi_str_mv | 10.2500/ajra.2011.25.3564 |
| format | Article |
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After a nasal smear culture, the nasal lavage fluid and NP tissue were obtained from 30 CRS with NP (CRSwNP) patients. The control samples were taken from 10 normal individuals. The presence of S. aureus exotoxin A (SEA) and toxic shock syndrome toxin 1 (TSST-1) was verified through a polymerase chain reaction (PCR) analysis and immunohistochemical staining. Immunohistochemical staining for CD4(+) and CD8(+) T cells was performed, followed by hematoxylin and eosin (H&E) staining for eosinophils.
The PCR analysis and immunohistochemical staining for SEA and TSST-1 showed a significantly higher expression in the NP group when compared with that in the control group. Furthermore, the number of T cells and eosinophils were notably increased in the NP group when compared with that in the control group. Significant correlation was observed between the results of the culture, PCR, and immunohistochemical staining in the NP group. Moreover, the number of T cells and eosinophils was significantly increased in the positive experimental groups, when compared with that in the control group.
Our results indicate that there was, indeed, some correlation between the expression of SE in the nasal cavity and the increased number of T cells and eosinophils in NPs.</description><identifier>ISSN: 1945-8924</identifier><identifier>EISSN: 1945-8932</identifier><identifier>DOI: 10.2500/ajra.2011.25.3564</identifier><identifier>PMID: 21711967</identifier><language>eng</language><publisher>United States</publisher><subject>Adult ; Bacterial Proteins - genetics ; Bacterial Proteins - immunology ; Bacterial Proteins - metabolism ; Bacterial Toxins - genetics ; Bacterial Toxins - immunology ; Bacterial Toxins - metabolism ; CD4-Positive T-Lymphocytes - pathology ; CD8-Positive T-Lymphocytes - pathology ; Cell Count ; Chronic Disease ; Enterotoxins - genetics ; Enterotoxins - immunology ; Enterotoxins - metabolism ; Eosinophils - pathology ; Exotoxins - genetics ; Exotoxins - immunology ; Exotoxins - metabolism ; Female ; Gene Expression Regulation, Bacterial ; Humans ; Male ; Middle Aged ; Nasal Polyps - metabolism ; Nasal Polyps - pathology ; Rhinitis - immunology ; Rhinitis - microbiology ; Rhinitis - physiopathology ; Sinusitis - immunology ; Sinusitis - microbiology ; Sinusitis - physiopathology ; Staphylococcus aureus - genetics ; Staphylococcus aureus - metabolism ; Superantigens - genetics ; Superantigens - immunology ; Superantigens - metabolism</subject><ispartof>American journal of rhinology & allergy, 2011-01, Vol.25 (1), p.19-24</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c300t-c4afaecaf68fe7d91fe5b18a1db995e40187a9b1062ad54623cb018806b8fb313</citedby><cites>FETCH-LOGICAL-c300t-c4afaecaf68fe7d91fe5b18a1db995e40187a9b1062ad54623cb018806b8fb313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21711967$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Seon Tae</creatorcontrib><creatorcontrib>Chung, Seung Won</creatorcontrib><creatorcontrib>Jung, Joo Hyun</creatorcontrib><creatorcontrib>Ha, Jong Su</creatorcontrib><creatorcontrib>Kang, Il Gyu</creatorcontrib><title>Association of T cells and eosinophils with Staphylococcus aureus exotoxin A and toxic shock syndrome toxin 1 in nasal polyps</title><title>American journal of rhinology & allergy</title><addtitle>Am J Rhinol Allergy</addtitle><description>Staphylococcus aureus exotoxins (SEs) have been implicated in the pathogenesis of nasal polyps (NPs) in patients with chronic rhinosinusitis (CRS). The aim of this study was to identify the correlation between SEs and T cells in NPs.
After a nasal smear culture, the nasal lavage fluid and NP tissue were obtained from 30 CRS with NP (CRSwNP) patients. The control samples were taken from 10 normal individuals. The presence of S. aureus exotoxin A (SEA) and toxic shock syndrome toxin 1 (TSST-1) was verified through a polymerase chain reaction (PCR) analysis and immunohistochemical staining. Immunohistochemical staining for CD4(+) and CD8(+) T cells was performed, followed by hematoxylin and eosin (H&E) staining for eosinophils.
The PCR analysis and immunohistochemical staining for SEA and TSST-1 showed a significantly higher expression in the NP group when compared with that in the control group. Furthermore, the number of T cells and eosinophils were notably increased in the NP group when compared with that in the control group. Significant correlation was observed between the results of the culture, PCR, and immunohistochemical staining in the NP group. Moreover, the number of T cells and eosinophils was significantly increased in the positive experimental groups, when compared with that in the control group.
Our results indicate that there was, indeed, some correlation between the expression of SE in the nasal cavity and the increased number of T cells and eosinophils in NPs.</description><subject>Adult</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacterial Toxins - immunology</subject><subject>Bacterial Toxins - metabolism</subject><subject>CD4-Positive T-Lymphocytes - pathology</subject><subject>CD8-Positive T-Lymphocytes - pathology</subject><subject>Cell Count</subject><subject>Chronic Disease</subject><subject>Enterotoxins - genetics</subject><subject>Enterotoxins - immunology</subject><subject>Enterotoxins - metabolism</subject><subject>Eosinophils - pathology</subject><subject>Exotoxins - genetics</subject><subject>Exotoxins - immunology</subject><subject>Exotoxins - metabolism</subject><subject>Female</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Nasal Polyps - metabolism</subject><subject>Nasal Polyps - pathology</subject><subject>Rhinitis - immunology</subject><subject>Rhinitis - microbiology</subject><subject>Rhinitis - physiopathology</subject><subject>Sinusitis - immunology</subject><subject>Sinusitis - microbiology</subject><subject>Sinusitis - physiopathology</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - metabolism</subject><subject>Superantigens - genetics</subject><subject>Superantigens - immunology</subject><subject>Superantigens - metabolism</subject><issn>1945-8924</issn><issn>1945-8932</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOwzAQRS0EoqXwAWyQd6xSPInzWlYVL6kSC8rachxHSUni4ElEs-DfcWjpZmbu6N6R5hByC2zph4w9yJ2VS58BOLkMwoifkTmkPPSSNPDPT7PPZ-QKccdYxEMOl2TmQwyQRvGc_KwQjapkX5mWmoJuqdJ1jVS2OdUGq9Z0ZeX0d9WX9L2XXTnWRhmlBucZrHZN701v9lVLV3-paVYUS6M-KY5tbk2j6cEA1JVWoqxpZ-qxw2tyUcga9c2xL8jH0-N2_eJt3p5f16uNpwLGek9xWUitZBElhY7zFAodZpBIyLM0DTVnkMQyzYBFvsxDHvmBytwuYVGWFFkAwYLcH-521nwNGnvRVDg9KlttBhRJzP00AuDOCQensgbR6kJ0tmqkHQUwMUEXE3QxQXdSTNBd5u54fcganZ8S_5SDX-jDgJM</recordid><startdate>201101</startdate><enddate>201101</enddate><creator>Kim, Seon Tae</creator><creator>Chung, Seung Won</creator><creator>Jung, Joo Hyun</creator><creator>Ha, Jong Su</creator><creator>Kang, Il Gyu</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201101</creationdate><title>Association of T cells and eosinophils with Staphylococcus aureus exotoxin A and toxic shock syndrome toxin 1 in nasal polyps</title><author>Kim, Seon Tae ; Chung, Seung Won ; Jung, Joo Hyun ; Ha, Jong Su ; Kang, Il Gyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c300t-c4afaecaf68fe7d91fe5b18a1db995e40187a9b1062ad54623cb018806b8fb313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adult</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Toxins - genetics</topic><topic>Bacterial Toxins - immunology</topic><topic>Bacterial Toxins - metabolism</topic><topic>CD4-Positive T-Lymphocytes - pathology</topic><topic>CD8-Positive T-Lymphocytes - pathology</topic><topic>Cell Count</topic><topic>Chronic Disease</topic><topic>Enterotoxins - genetics</topic><topic>Enterotoxins - immunology</topic><topic>Enterotoxins - metabolism</topic><topic>Eosinophils - pathology</topic><topic>Exotoxins - genetics</topic><topic>Exotoxins - immunology</topic><topic>Exotoxins - metabolism</topic><topic>Female</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Nasal Polyps - metabolism</topic><topic>Nasal Polyps - pathology</topic><topic>Rhinitis - immunology</topic><topic>Rhinitis - microbiology</topic><topic>Rhinitis - physiopathology</topic><topic>Sinusitis - immunology</topic><topic>Sinusitis - microbiology</topic><topic>Sinusitis - physiopathology</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - metabolism</topic><topic>Superantigens - genetics</topic><topic>Superantigens - immunology</topic><topic>Superantigens - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Seon Tae</creatorcontrib><creatorcontrib>Chung, Seung Won</creatorcontrib><creatorcontrib>Jung, Joo Hyun</creatorcontrib><creatorcontrib>Ha, Jong Su</creatorcontrib><creatorcontrib>Kang, Il Gyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of rhinology & allergy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Seon Tae</au><au>Chung, Seung Won</au><au>Jung, Joo Hyun</au><au>Ha, Jong Su</au><au>Kang, Il Gyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Association of T cells and eosinophils with Staphylococcus aureus exotoxin A and toxic shock syndrome toxin 1 in nasal polyps</atitle><jtitle>American journal of rhinology & allergy</jtitle><addtitle>Am J Rhinol Allergy</addtitle><date>2011-01</date><risdate>2011</risdate><volume>25</volume><issue>1</issue><spage>19</spage><epage>24</epage><pages>19-24</pages><issn>1945-8924</issn><eissn>1945-8932</eissn><abstract>Staphylococcus aureus exotoxins (SEs) have been implicated in the pathogenesis of nasal polyps (NPs) in patients with chronic rhinosinusitis (CRS). The aim of this study was to identify the correlation between SEs and T cells in NPs.
After a nasal smear culture, the nasal lavage fluid and NP tissue were obtained from 30 CRS with NP (CRSwNP) patients. The control samples were taken from 10 normal individuals. The presence of S. aureus exotoxin A (SEA) and toxic shock syndrome toxin 1 (TSST-1) was verified through a polymerase chain reaction (PCR) analysis and immunohistochemical staining. Immunohistochemical staining for CD4(+) and CD8(+) T cells was performed, followed by hematoxylin and eosin (H&E) staining for eosinophils.
The PCR analysis and immunohistochemical staining for SEA and TSST-1 showed a significantly higher expression in the NP group when compared with that in the control group. Furthermore, the number of T cells and eosinophils were notably increased in the NP group when compared with that in the control group. Significant correlation was observed between the results of the culture, PCR, and immunohistochemical staining in the NP group. Moreover, the number of T cells and eosinophils was significantly increased in the positive experimental groups, when compared with that in the control group.
Our results indicate that there was, indeed, some correlation between the expression of SE in the nasal cavity and the increased number of T cells and eosinophils in NPs.</abstract><cop>United States</cop><pmid>21711967</pmid><doi>10.2500/ajra.2011.25.3564</doi><tpages>6</tpages></addata></record> |
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| subjects | Adult Bacterial Proteins - genetics Bacterial Proteins - immunology Bacterial Proteins - metabolism Bacterial Toxins - genetics Bacterial Toxins - immunology Bacterial Toxins - metabolism CD4-Positive T-Lymphocytes - pathology CD8-Positive T-Lymphocytes - pathology Cell Count Chronic Disease Enterotoxins - genetics Enterotoxins - immunology Enterotoxins - metabolism Eosinophils - pathology Exotoxins - genetics Exotoxins - immunology Exotoxins - metabolism Female Gene Expression Regulation, Bacterial Humans Male Middle Aged Nasal Polyps - metabolism Nasal Polyps - pathology Rhinitis - immunology Rhinitis - microbiology Rhinitis - physiopathology Sinusitis - immunology Sinusitis - microbiology Sinusitis - physiopathology Staphylococcus aureus - genetics Staphylococcus aureus - metabolism Superantigens - genetics Superantigens - immunology Superantigens - metabolism |
| title | Association of T cells and eosinophils with Staphylococcus aureus exotoxin A and toxic shock syndrome toxin 1 in nasal polyps |
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