Multiplexed protein analysis using encoded antibody-conjugated microbeads

We describe a method for multiplexed analysis of proteins using fluorescently encoded microbeads. The sensitivity of our method is comparable to the sensitivity obtained by enzyme-linked immunosorbent assay while only 5 µl sample volumes are needed. Streptavidin-coated, 1 µm beads are encoded with a...

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Veröffentlicht in:	Journal of the Royal Society interface 2011-08, Vol.8 (61), p.1104-1113
Hauptverfasser:	Theilacker, Nora, Roller, Eric E., Barbee, Kristopher D., Franzreb, Matthias, Huang, Xiaohua
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies - chemistry Biomarkers, Tumor - analysis Combinatorial Encoding Cytokines - analysis Fluorescent Dyes - chemistry Humans Mice Microbeads Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microspheres Multiplexed Protein Immunoassays Sensitivity and Specificity
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