Vaccinia virus as a vaccine delivery system for marsupial wildlife
Abstract Vaccines based on recombinant poxviruses have proved successful in controlling diseases such as rabies and plague in wild eutherian mammals. They have also been trialled experimentally as delivery agents for fertility-control vaccines in rodents and foxes. In some countries, marsupial mamma...
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| Veröffentlicht in: | Vaccine 2011-06, Vol.29 (28), p.4537-4543 |
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| creator | Cross, Martin L Fleming, Stephen B Cowan, Phil E Scobie, Susie Whelan, Ellena Prada, Diana Mercer, Andrew A Duckworth, Janine A |
| description | Abstract Vaccines based on recombinant poxviruses have proved successful in controlling diseases such as rabies and plague in wild eutherian mammals. They have also been trialled experimentally as delivery agents for fertility-control vaccines in rodents and foxes. In some countries, marsupial mammals represent a wildlife disease reservoir or a threat to conservation values but, as yet there has been no bespoke study of efficacy or immunogenicity of a poxvirus-based vaccine delivery system in a marsupial. Here, we report a study of the potential for vaccination using vaccinia virus in the Australian brushtail possum Trichosurus vulpecula , an introduced pest species in New Zealand. Parent-strain vaccinia virus (Lister) infected 8/8 possums following delivery of virus to the oral cavity and outer nares surfaces (oronasal immunisation), and persisted in the mucosal epithelium around the palatine tonsils for up to 2 weeks post-exposure. A recombinant vaccinia virus construct (VV399, which expresses the Eg95 antigen of the hydatid disease parasite Echinococcus granulosus ) was shown to infect 10/15 possums after a single-dose oronasal delivery and to also persist. Both parent vaccinia virus and the VV399 construct virus induced peripheral blood lymphocyte reactivity against viral antigens in possums, first apparent at 4 weeks post-exposure and still detectable at 4 months post-exposure. Serum antibody reactivity to Eg95 was recorded in 7/8 possums which received a single dose of the VV399 construct and 7/7 animals which received triple-dose delivery, with titre end-points in the latter case exceeding 1/4000 dilution. This study demonstrates that vaccinia virus will readily infect possums via a delivery means used to deploy wildlife vaccines, and in doing is capable of generating immune reactivity against viral and heterologous antigens. This highlights the future potential of recombinant vaccinia virus as a vaccine delivery system in marsupial wildlife. |
| doi_str_mv | 10.1016/j.vaccine.2011.04.093 |
| format | Article |
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They have also been trialled experimentally as delivery agents for fertility-control vaccines in rodents and foxes. In some countries, marsupial mammals represent a wildlife disease reservoir or a threat to conservation values but, as yet there has been no bespoke study of efficacy or immunogenicity of a poxvirus-based vaccine delivery system in a marsupial. Here, we report a study of the potential for vaccination using vaccinia virus in the Australian brushtail possum Trichosurus vulpecula , an introduced pest species in New Zealand. Parent-strain vaccinia virus (Lister) infected 8/8 possums following delivery of virus to the oral cavity and outer nares surfaces (oronasal immunisation), and persisted in the mucosal epithelium around the palatine tonsils for up to 2 weeks post-exposure. A recombinant vaccinia virus construct (VV399, which expresses the Eg95 antigen of the hydatid disease parasite Echinococcus granulosus ) was shown to infect 10/15 possums after a single-dose oronasal delivery and to also persist. Both parent vaccinia virus and the VV399 construct virus induced peripheral blood lymphocyte reactivity against viral antigens in possums, first apparent at 4 weeks post-exposure and still detectable at 4 months post-exposure. Serum antibody reactivity to Eg95 was recorded in 7/8 possums which received a single dose of the VV399 construct and 7/7 animals which received triple-dose delivery, with titre end-points in the latter case exceeding 1/4000 dilution. This study demonstrates that vaccinia virus will readily infect possums via a delivery means used to deploy wildlife vaccines, and in doing is capable of generating immune reactivity against viral and heterologous antigens. This highlights the future potential of recombinant vaccinia virus as a vaccine delivery system in marsupial wildlife.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/j.vaccine.2011.04.093</identifier><identifier>PMID: 21570435</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Administration, Oral ; Allergy and Immunology ; Animal reproduction ; Animals ; Animals, Wild - virology ; Antibodies, Helminth - blood ; Antigens ; Antigens, Helminth - genetics ; Antigens, Helminth - immunology ; Antigens, Helminth - metabolism ; Applied microbiology ; Biological and medical sciences ; Disease ; Disease control ; Diseases caused by cestodes ; Drug Delivery Systems - methods ; Drug Delivery Systems - veterinary ; Echinococcoses ; Echinococcosis - immunology ; Echinococcosis - prevention & control ; Echinococcus granulosus ; Echinococcus granulosus - genetics ; Echinococcus granulosus - immunology ; Echinococcus granulosus - metabolism ; Female ; Fertility ; Fertility-control ; Fundamental and applied biological sciences. Psychology ; Genetic Vectors ; Helminth Proteins - genetics ; Helminth Proteins - immunology ; Helminth Proteins - metabolism ; Helminthic diseases ; Hydatid disease ; Immunization ; Immunogenicity ; Infections ; Infectious diseases ; Introduced species ; Livestock ; Lymphocyte Activation ; Lymphocytes ; Mammals ; Marsupial ; Marsupials ; Medical sciences ; Microbiology ; Miscellaneous ; Oronasal vaccine ; Parasitic diseases ; Poxvirus ; Reproductive system ; Trichosurus - virology ; Trichosurus vulpecula ; Vaccines ; Vaccines - administration & dosage ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccinia ; Vaccinia virus ; Vaccinia virus - genetics ; Vaccinia virus - immunology ; Vaccinia virus - pathogenicity ; Virology ; Wildlife</subject><ispartof>Vaccine, 2011-06, Vol.29 (28), p.4537-4543</ispartof><rights>Elsevier Ltd</rights><rights>2011 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Jun 20, 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-4a23910a8886f605ad4dc134f61a689129fece5a7a1ec9904041e17353ab3d103</citedby><cites>FETCH-LOGICAL-c509t-4a23910a8886f605ad4dc134f61a689129fece5a7a1ec9904041e17353ab3d103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1618947600?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,64361,64363,64365,65309,72215</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24282146$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21570435$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cross, Martin L</creatorcontrib><creatorcontrib>Fleming, Stephen B</creatorcontrib><creatorcontrib>Cowan, Phil E</creatorcontrib><creatorcontrib>Scobie, Susie</creatorcontrib><creatorcontrib>Whelan, Ellena</creatorcontrib><creatorcontrib>Prada, Diana</creatorcontrib><creatorcontrib>Mercer, Andrew A</creatorcontrib><creatorcontrib>Duckworth, Janine A</creatorcontrib><title>Vaccinia virus as a vaccine delivery system for marsupial wildlife</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Abstract Vaccines based on recombinant poxviruses have proved successful in controlling diseases such as rabies and plague in wild eutherian mammals. They have also been trialled experimentally as delivery agents for fertility-control vaccines in rodents and foxes. In some countries, marsupial mammals represent a wildlife disease reservoir or a threat to conservation values but, as yet there has been no bespoke study of efficacy or immunogenicity of a poxvirus-based vaccine delivery system in a marsupial. Here, we report a study of the potential for vaccination using vaccinia virus in the Australian brushtail possum Trichosurus vulpecula , an introduced pest species in New Zealand. Parent-strain vaccinia virus (Lister) infected 8/8 possums following delivery of virus to the oral cavity and outer nares surfaces (oronasal immunisation), and persisted in the mucosal epithelium around the palatine tonsils for up to 2 weeks post-exposure. A recombinant vaccinia virus construct (VV399, which expresses the Eg95 antigen of the hydatid disease parasite Echinococcus granulosus ) was shown to infect 10/15 possums after a single-dose oronasal delivery and to also persist. Both parent vaccinia virus and the VV399 construct virus induced peripheral blood lymphocyte reactivity against viral antigens in possums, first apparent at 4 weeks post-exposure and still detectable at 4 months post-exposure. Serum antibody reactivity to Eg95 was recorded in 7/8 possums which received a single dose of the VV399 construct and 7/7 animals which received triple-dose delivery, with titre end-points in the latter case exceeding 1/4000 dilution. This study demonstrates that vaccinia virus will readily infect possums via a delivery means used to deploy wildlife vaccines, and in doing is capable of generating immune reactivity against viral and heterologous antigens. This highlights the future potential of recombinant vaccinia virus as a vaccine delivery system in marsupial wildlife.</description><subject>Administration, Oral</subject><subject>Allergy and Immunology</subject><subject>Animal reproduction</subject><subject>Animals</subject><subject>Animals, Wild - virology</subject><subject>Antibodies, Helminth - blood</subject><subject>Antigens</subject><subject>Antigens, Helminth - genetics</subject><subject>Antigens, Helminth - immunology</subject><subject>Antigens, Helminth - metabolism</subject><subject>Applied microbiology</subject><subject>Biological and medical sciences</subject><subject>Disease</subject><subject>Disease control</subject><subject>Diseases caused by cestodes</subject><subject>Drug Delivery Systems - methods</subject><subject>Drug Delivery Systems - veterinary</subject><subject>Echinococcoses</subject><subject>Echinococcosis - immunology</subject><subject>Echinococcosis - prevention & control</subject><subject>Echinococcus granulosus</subject><subject>Echinococcus granulosus - genetics</subject><subject>Echinococcus granulosus - immunology</subject><subject>Echinococcus granulosus - metabolism</subject><subject>Female</subject><subject>Fertility</subject><subject>Fertility-control</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Vectors</subject><subject>Helminth Proteins - genetics</subject><subject>Helminth Proteins - immunology</subject><subject>Helminth Proteins - metabolism</subject><subject>Helminthic diseases</subject><subject>Hydatid disease</subject><subject>Immunization</subject><subject>Immunogenicity</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Introduced species</subject><subject>Livestock</subject><subject>Lymphocyte Activation</subject><subject>Lymphocytes</subject><subject>Mammals</subject><subject>Marsupial</subject><subject>Marsupials</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Oronasal vaccine</subject><subject>Parasitic diseases</subject><subject>Poxvirus</subject><subject>Reproductive system</subject><subject>Trichosurus - virology</subject><subject>Trichosurus vulpecula</subject><subject>Vaccines</subject><subject>Vaccines - administration & dosage</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</subject><subject>Vaccinia</subject><subject>Vaccinia virus</subject><subject>Vaccinia virus - genetics</subject><subject>Vaccinia virus - immunology</subject><subject>Vaccinia virus - pathogenicity</subject><subject>Virology</subject><subject>Wildlife</subject><issn>0264-410X</issn><issn>1873-2518</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqNkk2L1EAQhhtxccfVn6AERDwlVvVX0hcXXdRdWPDgB96a3k4FeswkY_dkZP69HSe6sBcXCury1Ntd71uMPUOoEFC_Xld7530YqOKAWIGswIgHbIVNLUqusHnIVsC1LCXC91P2OKU1ACiB5hE75ahqkEKt2Ltvf1SCK_YhTqlwuYpFuWipD3uKhyId0o42RTfGYuNimrbB9cWv0Ld96OgJO-lcn-jp0s_Y1w_vv1xcltefPl5dvL0uvQKzK6XjwiC4pml0p0G5VrYehew0Ot0Y5KYjT8rVDskbAxIkEtZCCXcjWgRxxl4ddbdx_DlR2tlNSJ763g00Tsk2tUSTC-9BcsWV4bPmizvkepzikNewqLExstYwU-pI-TimFKmz2xiyEQeLYOc07Nountk5DQvS5jTy3PNFfbrZUPtv6q_9GXi5AC5513fRDT6kW07yhqPUmTs_cpT93QeKNvlAg6c2RPI7247hv195c0fB9zn2_OgPOlC63dombsF-nk9nvhxEAC3zTf0GUEK9Jg</recordid><startdate>20110620</startdate><enddate>20110620</enddate><creator>Cross, Martin L</creator><creator>Fleming, Stephen B</creator><creator>Cowan, Phil E</creator><creator>Scobie, Susie</creator><creator>Whelan, Ellena</creator><creator>Prada, Diana</creator><creator>Mercer, Andrew A</creator><creator>Duckworth, Janine A</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7RV</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88C</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M0T</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20110620</creationdate><title>Vaccinia virus as a vaccine delivery system for marsupial wildlife</title><author>Cross, Martin L ; Fleming, Stephen B ; Cowan, Phil E ; Scobie, Susie ; Whelan, Ellena ; Prada, Diana ; Mercer, Andrew A ; Duckworth, Janine A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-4a23910a8886f605ad4dc134f61a689129fece5a7a1ec9904041e17353ab3d103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Administration, Oral</topic><topic>Allergy and Immunology</topic><topic>Animal reproduction</topic><topic>Animals</topic><topic>Animals, Wild - virology</topic><topic>Antibodies, Helminth - blood</topic><topic>Antigens</topic><topic>Antigens, Helminth - genetics</topic><topic>Antigens, Helminth - immunology</topic><topic>Antigens, Helminth - metabolism</topic><topic>Applied microbiology</topic><topic>Biological and medical sciences</topic><topic>Disease</topic><topic>Disease control</topic><topic>Diseases caused by cestodes</topic><topic>Drug Delivery Systems - methods</topic><topic>Drug Delivery Systems - veterinary</topic><topic>Echinococcoses</topic><topic>Echinococcosis - immunology</topic><topic>Echinococcosis - prevention & control</topic><topic>Echinococcus granulosus</topic><topic>Echinococcus granulosus - genetics</topic><topic>Echinococcus granulosus - immunology</topic><topic>Echinococcus granulosus - metabolism</topic><topic>Female</topic><topic>Fertility</topic><topic>Fertility-control</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Vectors</topic><topic>Helminth Proteins - genetics</topic><topic>Helminth Proteins - immunology</topic><topic>Helminth Proteins - metabolism</topic><topic>Helminthic diseases</topic><topic>Hydatid disease</topic><topic>Immunization</topic><topic>Immunogenicity</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Introduced species</topic><topic>Livestock</topic><topic>Lymphocyte Activation</topic><topic>Lymphocytes</topic><topic>Mammals</topic><topic>Marsupial</topic><topic>Marsupials</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Oronasal vaccine</topic><topic>Parasitic diseases</topic><topic>Poxvirus</topic><topic>Reproductive system</topic><topic>Trichosurus - virology</topic><topic>Trichosurus vulpecula</topic><topic>Vaccines</topic><topic>Vaccines - administration & dosage</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)</topic><topic>Vaccinia</topic><topic>Vaccinia virus</topic><topic>Vaccinia virus - 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They have also been trialled experimentally as delivery agents for fertility-control vaccines in rodents and foxes. In some countries, marsupial mammals represent a wildlife disease reservoir or a threat to conservation values but, as yet there has been no bespoke study of efficacy or immunogenicity of a poxvirus-based vaccine delivery system in a marsupial. Here, we report a study of the potential for vaccination using vaccinia virus in the Australian brushtail possum Trichosurus vulpecula , an introduced pest species in New Zealand. Parent-strain vaccinia virus (Lister) infected 8/8 possums following delivery of virus to the oral cavity and outer nares surfaces (oronasal immunisation), and persisted in the mucosal epithelium around the palatine tonsils for up to 2 weeks post-exposure. A recombinant vaccinia virus construct (VV399, which expresses the Eg95 antigen of the hydatid disease parasite Echinococcus granulosus ) was shown to infect 10/15 possums after a single-dose oronasal delivery and to also persist. Both parent vaccinia virus and the VV399 construct virus induced peripheral blood lymphocyte reactivity against viral antigens in possums, first apparent at 4 weeks post-exposure and still detectable at 4 months post-exposure. Serum antibody reactivity to Eg95 was recorded in 7/8 possums which received a single dose of the VV399 construct and 7/7 animals which received triple-dose delivery, with titre end-points in the latter case exceeding 1/4000 dilution. This study demonstrates that vaccinia virus will readily infect possums via a delivery means used to deploy wildlife vaccines, and in doing is capable of generating immune reactivity against viral and heterologous antigens. This highlights the future potential of recombinant vaccinia virus as a vaccine delivery system in marsupial wildlife.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>21570435</pmid><doi>10.1016/j.vaccine.2011.04.093</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0264-410X |
| ispartof | Vaccine, 2011-06, Vol.29 (28), p.4537-4543 |
| issn | 0264-410X 1873-2518 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_874194191 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; ProQuest Central UK/Ireland |
| subjects | Administration, Oral Allergy and Immunology Animal reproduction Animals Animals, Wild - virology Antibodies, Helminth - blood Antigens Antigens, Helminth - genetics Antigens, Helminth - immunology Antigens, Helminth - metabolism Applied microbiology Biological and medical sciences Disease Disease control Diseases caused by cestodes Drug Delivery Systems - methods Drug Delivery Systems - veterinary Echinococcoses Echinococcosis - immunology Echinococcosis - prevention & control Echinococcus granulosus Echinococcus granulosus - genetics Echinococcus granulosus - immunology Echinococcus granulosus - metabolism Female Fertility Fertility-control Fundamental and applied biological sciences. Psychology Genetic Vectors Helminth Proteins - genetics Helminth Proteins - immunology Helminth Proteins - metabolism Helminthic diseases Hydatid disease Immunization Immunogenicity Infections Infectious diseases Introduced species Livestock Lymphocyte Activation Lymphocytes Mammals Marsupial Marsupials Medical sciences Microbiology Miscellaneous Oronasal vaccine Parasitic diseases Poxvirus Reproductive system Trichosurus - virology Trichosurus vulpecula Vaccines Vaccines - administration & dosage Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccinia Vaccinia virus Vaccinia virus - genetics Vaccinia virus - immunology Vaccinia virus - pathogenicity Virology Wildlife |
| title | Vaccinia virus as a vaccine delivery system for marsupial wildlife |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T17%3A14%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Vaccinia%20virus%20as%20a%20vaccine%20delivery%20system%20for%20marsupial%20wildlife&rft.jtitle=Vaccine&rft.au=Cross,%20Martin%20L&rft.date=2011-06-20&rft.volume=29&rft.issue=28&rft.spage=4537&rft.epage=4543&rft.pages=4537-4543&rft.issn=0264-410X&rft.eissn=1873-2518&rft.coden=VACCDE&rft_id=info:doi/10.1016/j.vaccine.2011.04.093&rft_dat=%3Cproquest_cross%3E874194191%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1618947600&rft_id=info:pmid/21570435&rft_els_id=S0264410X11006487&rfr_iscdi=true |