Construction of an Escherichia coli mutant producing monophosphoryl lipid A

Lipid A is a major component in the outer membrane of most Gram-negative bacteria. Monophosphoryl lipid A contains no phosphate group at 1-position and can be used as an adjuvant. We constructed an Escherichia coli mutant CW001 by integrating a gene lpxE into the chromosome of E. coli W3110. The gen...

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Veröffentlicht in:	Biotechnology letters 2011-05, Vol.33 (5), p.1013-1019
Hauptverfasser:	Chen, Jiuzhou, Tao, Guanjun, Wang, Xiaoyuan
Format:	Artikel
Sprache:	eng
Schlagworte:	Adjuvants Applied Microbiology Bacteria Biochemistry Biological and medical sciences Biomedical and Life Sciences Biotechnology Chromatography, Thin Layer Chromosomes Construction E coli Enzymes Escherichia coli Escherichia coli - chemistry Escherichia coli - genetics Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Genes Gram-negative bacteria Ionization Life Sciences lipid A Lipid A - analogs & derivatives Lipid A - biosynthesis Lipids Mass spectrometry Microbiology mutants new methods Original Research Paper Phosphoric Monoester Hydrolases - genetics Phosphoric Monoester Hydrolases - metabolism Recombination, Genetic Salmonella - enzymology Salmonella - genetics Spectrometry, Mass, Electrospray Ionization Thin layer chromatography Transformation, Bacterial
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