Polymorphism of Paramecium pentaurelia (Ciliophora, Oligohymenophorea) strains revealed by rDNA and mtDNA sequences
Paramecium pentaurelia is one of 15 known sibling species of the Paramecium aurelia complex. It is recognized as a species showing no intra-specific differentiation on the basis of molecular fingerprint analyses, whereas the majority of other species are polymorphic. This study aimed at assessing ge...
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| Veröffentlicht in: | European journal of protistology 2011-05, Vol.47 (2), p.138-143 |
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| creator | Przyboś, Ewa Tarcz, Sebastian Greczek-Stachura, Magdalena Surmacz, Marta |
| description | Paramecium pentaurelia is one of 15 known sibling species of the Paramecium aurelia complex. It is recognized as a species showing no intra-specific differentiation on the basis of molecular fingerprint analyses, whereas the majority of other species are polymorphic. This study aimed at assessing genetic polymorphism within P. pentaurelia including new strains recently found in Poland (originating from two water bodies, different years, seasons, and clones of one strain) as well as strains collected from distant habitats (USA, Europe, Asia), and strains representing other species of the complex. We compared two DNA fragments: partial sequences (349bp) of the LSU rDNA and partial sequences (618bp) of cytochrome B gene. A correlation between the geographical origin of the strains and the genetic characteristics of their genotypes was not observed. Different genotypes were found in Kraków in two types of water bodies (Opatkowice—natural pond; Jordan's Park—artificial pond). Haplotype diversity within a single water body was not recorded. Likewise, seasonal haplotype differences between the strains within the artificial water body, as well as differences between clones originating from one strain, were not detected. The clustering of some strains belonging to different species was observed in the phylogenies. |
| doi_str_mv | 10.1016/j.ejop.2010.11.001 |
| format | Article |
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It is recognized as a species showing no intra-specific differentiation on the basis of molecular fingerprint analyses, whereas the majority of other species are polymorphic. This study aimed at assessing genetic polymorphism within P. pentaurelia including new strains recently found in Poland (originating from two water bodies, different years, seasons, and clones of one strain) as well as strains collected from distant habitats (USA, Europe, Asia), and strains representing other species of the complex. We compared two DNA fragments: partial sequences (349bp) of the LSU rDNA and partial sequences (618bp) of cytochrome B gene. A correlation between the geographical origin of the strains and the genetic characteristics of their genotypes was not observed. Different genotypes were found in Kraków in two types of water bodies (Opatkowice—natural pond; Jordan's Park—artificial pond). Haplotype diversity within a single water body was not recorded. Likewise, seasonal haplotype differences between the strains within the artificial water body, as well as differences between clones originating from one strain, were not detected. The clustering of some strains belonging to different species was observed in the phylogenies.</description><identifier>ISSN: 0932-4739</identifier><identifier>EISSN: 1618-0429</identifier><identifier>DOI: 10.1016/j.ejop.2010.11.001</identifier><identifier>PMID: 21282046</identifier><language>eng</language><publisher>Munich: Elsevier GmbH</publisher><subject>Asia ; Biological and medical sciences ; Ciliophora ; Cluster Analysis ; cytB mtDNA ; Cytochromes b - genetics ; DNA, Mitochondrial - chemistry ; DNA, Mitochondrial - genetics ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Europe ; Fundamental and applied biological sciences. Psychology ; Haplotypes ; Intra-specific diversity ; LSU rDNA ; Marine ; Molecular Sequence Data ; Paramecium ; Paramecium - classification ; Paramecium - genetics ; Paramecium aurelia ; Paramecium aurelia species complex ; Paramecium pentaurelia ; Phylogeny ; Phylogeography ; Polymorphism, Genetic ; Protozoa ; RNA, Ribosomal - genetics ; Sequence Analysis, DNA ; Systematics. Geographical distribution. Morphology. Cytology ; United States</subject><ispartof>European journal of protistology, 2011-05, Vol.47 (2), p.138-143</ispartof><rights>2010 Elsevier GmbH</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-a7001896fb93db142d867d964a58c79b71077fb2dda2d44e1524985e788830483</citedby><cites>FETCH-LOGICAL-c417t-a7001896fb93db142d867d964a58c79b71077fb2dda2d44e1524985e788830483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ejop.2010.11.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3538,27906,27907,45977</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24261572$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21282046$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Przyboś, Ewa</creatorcontrib><creatorcontrib>Tarcz, Sebastian</creatorcontrib><creatorcontrib>Greczek-Stachura, Magdalena</creatorcontrib><creatorcontrib>Surmacz, Marta</creatorcontrib><title>Polymorphism of Paramecium pentaurelia (Ciliophora, Oligohymenophorea) strains revealed by rDNA and mtDNA sequences</title><title>European journal of protistology</title><addtitle>Eur J Protistol</addtitle><description>Paramecium pentaurelia is one of 15 known sibling species of the Paramecium aurelia complex. It is recognized as a species showing no intra-specific differentiation on the basis of molecular fingerprint analyses, whereas the majority of other species are polymorphic. This study aimed at assessing genetic polymorphism within P. pentaurelia including new strains recently found in Poland (originating from two water bodies, different years, seasons, and clones of one strain) as well as strains collected from distant habitats (USA, Europe, Asia), and strains representing other species of the complex. We compared two DNA fragments: partial sequences (349bp) of the LSU rDNA and partial sequences (618bp) of cytochrome B gene. A correlation between the geographical origin of the strains and the genetic characteristics of their genotypes was not observed. Different genotypes were found in Kraków in two types of water bodies (Opatkowice—natural pond; Jordan's Park—artificial pond). Haplotype diversity within a single water body was not recorded. Likewise, seasonal haplotype differences between the strains within the artificial water body, as well as differences between clones originating from one strain, were not detected. The clustering of some strains belonging to different species was observed in the phylogenies.</description><subject>Asia</subject><subject>Biological and medical sciences</subject><subject>Ciliophora</subject><subject>Cluster Analysis</subject><subject>cytB mtDNA</subject><subject>Cytochromes b - genetics</subject><subject>DNA, Mitochondrial - chemistry</subject><subject>DNA, Mitochondrial - genetics</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Europe</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Haplotypes</subject><subject>Intra-specific diversity</subject><subject>LSU rDNA</subject><subject>Marine</subject><subject>Molecular Sequence Data</subject><subject>Paramecium</subject><subject>Paramecium - classification</subject><subject>Paramecium - genetics</subject><subject>Paramecium aurelia</subject><subject>Paramecium aurelia species complex</subject><subject>Paramecium pentaurelia</subject><subject>Phylogeny</subject><subject>Phylogeography</subject><subject>Polymorphism, Genetic</subject><subject>Protozoa</subject><subject>RNA, Ribosomal - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Systematics. Geographical distribution. Morphology. 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Psychology</topic><topic>Haplotypes</topic><topic>Intra-specific diversity</topic><topic>LSU rDNA</topic><topic>Marine</topic><topic>Molecular Sequence Data</topic><topic>Paramecium</topic><topic>Paramecium - classification</topic><topic>Paramecium - genetics</topic><topic>Paramecium aurelia</topic><topic>Paramecium aurelia species complex</topic><topic>Paramecium pentaurelia</topic><topic>Phylogeny</topic><topic>Phylogeography</topic><topic>Polymorphism, Genetic</topic><topic>Protozoa</topic><topic>RNA, Ribosomal - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Systematics. Geographical distribution. Morphology. 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It is recognized as a species showing no intra-specific differentiation on the basis of molecular fingerprint analyses, whereas the majority of other species are polymorphic. This study aimed at assessing genetic polymorphism within P. pentaurelia including new strains recently found in Poland (originating from two water bodies, different years, seasons, and clones of one strain) as well as strains collected from distant habitats (USA, Europe, Asia), and strains representing other species of the complex. We compared two DNA fragments: partial sequences (349bp) of the LSU rDNA and partial sequences (618bp) of cytochrome B gene. A correlation between the geographical origin of the strains and the genetic characteristics of their genotypes was not observed. Different genotypes were found in Kraków in two types of water bodies (Opatkowice—natural pond; Jordan's Park—artificial pond). Haplotype diversity within a single water body was not recorded. Likewise, seasonal haplotype differences between the strains within the artificial water body, as well as differences between clones originating from one strain, were not detected. The clustering of some strains belonging to different species was observed in the phylogenies.</abstract><cop>Munich</cop><pub>Elsevier GmbH</pub><pmid>21282046</pmid><doi>10.1016/j.ejop.2010.11.001</doi><tpages>6</tpages></addata></record> |
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| subjects | Asia Biological and medical sciences Ciliophora Cluster Analysis cytB mtDNA Cytochromes b - genetics DNA, Mitochondrial - chemistry DNA, Mitochondrial - genetics DNA, Protozoan - chemistry DNA, Protozoan - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Europe Fundamental and applied biological sciences. Psychology Haplotypes Intra-specific diversity LSU rDNA Marine Molecular Sequence Data Paramecium Paramecium - classification Paramecium - genetics Paramecium aurelia Paramecium aurelia species complex Paramecium pentaurelia Phylogeny Phylogeography Polymorphism, Genetic Protozoa RNA, Ribosomal - genetics Sequence Analysis, DNA Systematics. Geographical distribution. Morphology. Cytology United States |
| title | Polymorphism of Paramecium pentaurelia (Ciliophora, Oligohymenophorea) strains revealed by rDNA and mtDNA sequences |
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