Transgenic rice plants carrying RNA interference constructs of AOS (allene oxide synthase) genes show severe male sterility
With 3 figures ABSTRACT: Male sterility is an important trait for rice breeding programmes based on heterosis. To generate male‐sterile plants, we utilized RNAi technology with the promoters for two anther‐specific genes, Osc4 and Osg6b, and one for rice allene oxide synthase (OsAOS). AOS catalyzes...
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| Veröffentlicht in: | Plant breeding 2010-12, Vol.129 (6), p.647-651 |
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| creator | Bae, H.K Kang, H.‐G Kim, G.‐J Eu, H.‐J Oh, S.‐A Song, J.T Chung, I.‐K Eun, M.‐Y Park, S.K |
| description | With 3 figures ABSTRACT: Male sterility is an important trait for rice breeding programmes based on heterosis. To generate male‐sterile plants, we utilized RNAi technology with the promoters for two anther‐specific genes, Osc4 and Osg6b, and one for rice allene oxide synthase (OsAOS). AOS catalyzes the biosynthesis of jasmonic acid and plays an important role in anther development. The promoters isolated from Osc4 and Osg6b were introduced into pK7gwiwgL, a promoter‐less RNAi Gateway binary vector. Partial DNA fragments of OsAOS1 and OsAOS2 were combined into the Osc4::RNAi and Osg6b::RNAi cassettes by LR recombination. The resulting four RNAi constructs were transformed into rice calli by Agrobacterium. During the reproductive growth stage, 28% of OsAOS1 and 48% of OsAOS2 transgenic plants under the control of the Osg6b promoter exhibited a complete‐ or severe‐sterility phenotype compared with the normal wild type. Here, we report that RNA interference of OsAOS2 with the Osg6b promoter is the most effective system for obtaining engineered genic male sterility in rice. |
| doi_str_mv | 10.1111/j.1439-0523.2010.01784.x |
| format | Article |
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To generate male‐sterile plants, we utilized RNAi technology with the promoters for two anther‐specific genes, Osc4 and Osg6b, and one for rice allene oxide synthase (OsAOS). AOS catalyzes the biosynthesis of jasmonic acid and plays an important role in anther development. The promoters isolated from Osc4 and Osg6b were introduced into pK7gwiwgL, a promoter‐less RNAi Gateway binary vector. Partial DNA fragments of OsAOS1 and OsAOS2 were combined into the Osc4::RNAi and Osg6b::RNAi cassettes by LR recombination. The resulting four RNAi constructs were transformed into rice calli by Agrobacterium. During the reproductive growth stage, 28% of OsAOS1 and 48% of OsAOS2 transgenic plants under the control of the Osg6b promoter exhibited a complete‐ or severe‐sterility phenotype compared with the normal wild type. Here, we report that RNA interference of OsAOS2 with the Osg6b promoter is the most effective system for obtaining engineered genic male sterility in rice.</description><identifier>ISSN: 0179-9541</identifier><identifier>EISSN: 1439-0523</identifier><identifier>DOI: 10.1111/j.1439-0523.2010.01784.x</identifier><identifier>CODEN: PLABED</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Agrobacterium ; Agronomy. Soil science and plant productions ; anther-specific promoter ; Anthers ; Biological and medical sciences ; DNA ; Fundamental and applied biological sciences. Psychology ; Genetic engineering applications ; Genetics and breeding of economic plants ; Growth stage ; Heterosis ; Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia ; Jasmonic acid ; Male sterility ; Oryza sativa ; OsAOS ; oxides ; Plant breeding ; Plant breeding: fundamental aspects and methodology ; Promoters ; Recombination ; rice (Oryza sativa) ; RNA-mediated interference ; RNAi approach ; Transgenic plants</subject><ispartof>Plant breeding, 2010-12, Vol.129 (6), p.647-651</ispartof><rights>2010 Blackwell Verlag GmbH</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4374-879ff072badc9692f09e9e798d74b2402a2608d43d885c48a4814d96fe7d34353</citedby><cites>FETCH-LOGICAL-c4374-879ff072badc9692f09e9e798d74b2402a2608d43d885c48a4814d96fe7d34353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0523.2010.01784.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0523.2010.01784.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23454361$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Bae, H.K</creatorcontrib><creatorcontrib>Kang, H.‐G</creatorcontrib><creatorcontrib>Kim, G.‐J</creatorcontrib><creatorcontrib>Eu, H.‐J</creatorcontrib><creatorcontrib>Oh, S.‐A</creatorcontrib><creatorcontrib>Song, J.T</creatorcontrib><creatorcontrib>Chung, I.‐K</creatorcontrib><creatorcontrib>Eun, M.‐Y</creatorcontrib><creatorcontrib>Park, S.K</creatorcontrib><title>Transgenic rice plants carrying RNA interference constructs of AOS (allene oxide synthase) genes show severe male sterility</title><title>Plant breeding</title><description>With 3 figures ABSTRACT: Male sterility is an important trait for rice breeding programmes based on heterosis. To generate male‐sterile plants, we utilized RNAi technology with the promoters for two anther‐specific genes, Osc4 and Osg6b, and one for rice allene oxide synthase (OsAOS). AOS catalyzes the biosynthesis of jasmonic acid and plays an important role in anther development. The promoters isolated from Osc4 and Osg6b were introduced into pK7gwiwgL, a promoter‐less RNAi Gateway binary vector. Partial DNA fragments of OsAOS1 and OsAOS2 were combined into the Osc4::RNAi and Osg6b::RNAi cassettes by LR recombination. The resulting four RNAi constructs were transformed into rice calli by Agrobacterium. During the reproductive growth stage, 28% of OsAOS1 and 48% of OsAOS2 transgenic plants under the control of the Osg6b promoter exhibited a complete‐ or severe‐sterility phenotype compared with the normal wild type. Here, we report that RNA interference of OsAOS2 with the Osg6b promoter is the most effective system for obtaining engineered genic male sterility in rice.</description><subject>Agrobacterium</subject><subject>Agronomy. Soil science and plant productions</subject><subject>anther-specific promoter</subject><subject>Anthers</subject><subject>Biological and medical sciences</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering applications</subject><subject>Genetics and breeding of economic plants</subject><subject>Growth stage</subject><subject>Heterosis</subject><subject>Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia</subject><subject>Jasmonic acid</subject><subject>Male sterility</subject><subject>Oryza sativa</subject><subject>OsAOS</subject><subject>oxides</subject><subject>Plant breeding</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Promoters</subject><subject>Recombination</subject><subject>rice (Oryza sativa)</subject><subject>RNA-mediated interference</subject><subject>RNAi approach</subject><subject>Transgenic plants</subject><issn>0179-9541</issn><issn>1439-0523</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqNkEFv0zAYhi0EEmXwG_AFAYcUO3Zi-8ChTLCBpm7qNiFxsTznc-eSOsVOWSP-PA6ZesYXO1-e943zIIQpmdO8PmzmlDNVkKpk85LkKaFC8vnhCZodXzxFszxVhao4fY5epLQh4zMTM_TnJpqQ1hC8xdFbwLvWhD5ha2IcfFjj1XKBfeghOogQMmC7kPq4txnqHF5cXuN3pm0hAO4OvgGchtDfmwTvcW6FhNN994AT_M5xvDVtBnKZb30_vETPnGkTvHrcT9Dtl883p-fFxeXZ19PFRWE5E7yQQjlHRHlnGqtqVTqiQIFQshH8ruSkNGVNZMNZI2VluTRcUt6o2oFoGGcVO0Fvp95d7H7tIfV665OFNv8pdPukpeBUUlKOpJxIG7uUIji9i35r4qAp0aNuvdGjVT1a1aNu_U-3PuTom8ePmGRN67JW69MxXzJecVbTzH2cuAffwvDf_frq02o85Xwx5X32eDjmTfypa8FEpb8vz_Tqx7crKc-ZXmb-9cQ702mzjvlOt9e5mRGqKOeKsL95m6wZ</recordid><startdate>201012</startdate><enddate>201012</enddate><creator>Bae, H.K</creator><creator>Kang, H.‐G</creator><creator>Kim, G.‐J</creator><creator>Eu, H.‐J</creator><creator>Oh, S.‐A</creator><creator>Song, J.T</creator><creator>Chung, I.‐K</creator><creator>Eun, M.‐Y</creator><creator>Park, S.K</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201012</creationdate><title>Transgenic rice plants carrying RNA interference constructs of AOS (allene oxide synthase) genes show severe male sterility</title><author>Bae, H.K ; Kang, H.‐G ; Kim, G.‐J ; Eu, H.‐J ; Oh, S.‐A ; Song, J.T ; Chung, I.‐K ; Eun, M.‐Y ; Park, S.K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4374-879ff072badc9692f09e9e798d74b2402a2608d43d885c48a4814d96fe7d34353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Agrobacterium</topic><topic>Agronomy. Soil science and plant productions</topic><topic>anther-specific promoter</topic><topic>Anthers</topic><topic>Biological and medical sciences</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering applications</topic><topic>Genetics and breeding of economic plants</topic><topic>Growth stage</topic><topic>Heterosis</topic><topic>Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia</topic><topic>Jasmonic acid</topic><topic>Male sterility</topic><topic>Oryza sativa</topic><topic>OsAOS</topic><topic>oxides</topic><topic>Plant breeding</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Promoters</topic><topic>Recombination</topic><topic>rice (Oryza sativa)</topic><topic>RNA-mediated interference</topic><topic>RNAi approach</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bae, H.K</creatorcontrib><creatorcontrib>Kang, H.‐G</creatorcontrib><creatorcontrib>Kim, G.‐J</creatorcontrib><creatorcontrib>Eu, H.‐J</creatorcontrib><creatorcontrib>Oh, S.‐A</creatorcontrib><creatorcontrib>Song, J.T</creatorcontrib><creatorcontrib>Chung, I.‐K</creatorcontrib><creatorcontrib>Eun, M.‐Y</creatorcontrib><creatorcontrib>Park, S.K</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Plant breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bae, H.K</au><au>Kang, H.‐G</au><au>Kim, G.‐J</au><au>Eu, H.‐J</au><au>Oh, S.‐A</au><au>Song, J.T</au><au>Chung, I.‐K</au><au>Eun, M.‐Y</au><au>Park, S.K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transgenic rice plants carrying RNA interference constructs of AOS (allene oxide synthase) genes show severe male sterility</atitle><jtitle>Plant breeding</jtitle><date>2010-12</date><risdate>2010</risdate><volume>129</volume><issue>6</issue><spage>647</spage><epage>651</epage><pages>647-651</pages><issn>0179-9541</issn><eissn>1439-0523</eissn><coden>PLABED</coden><abstract>With 3 figures ABSTRACT: Male sterility is an important trait for rice breeding programmes based on heterosis. To generate male‐sterile plants, we utilized RNAi technology with the promoters for two anther‐specific genes, Osc4 and Osg6b, and one for rice allene oxide synthase (OsAOS). AOS catalyzes the biosynthesis of jasmonic acid and plays an important role in anther development. The promoters isolated from Osc4 and Osg6b were introduced into pK7gwiwgL, a promoter‐less RNAi Gateway binary vector. Partial DNA fragments of OsAOS1 and OsAOS2 were combined into the Osc4::RNAi and Osg6b::RNAi cassettes by LR recombination. The resulting four RNAi constructs were transformed into rice calli by Agrobacterium. During the reproductive growth stage, 28% of OsAOS1 and 48% of OsAOS2 transgenic plants under the control of the Osg6b promoter exhibited a complete‐ or severe‐sterility phenotype compared with the normal wild type. Here, we report that RNA interference of OsAOS2 with the Osg6b promoter is the most effective system for obtaining engineered genic male sterility in rice.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1439-0523.2010.01784.x</doi><tpages>5</tpages></addata></record> |
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| ispartof | Plant breeding, 2010-12, Vol.129 (6), p.647-651 |
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| subjects | Agrobacterium Agronomy. Soil science and plant productions anther-specific promoter Anthers Biological and medical sciences DNA Fundamental and applied biological sciences. Psychology Genetic engineering applications Genetics and breeding of economic plants Growth stage Heterosis Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia Jasmonic acid Male sterility Oryza sativa OsAOS oxides Plant breeding Plant breeding: fundamental aspects and methodology Promoters Recombination rice (Oryza sativa) RNA-mediated interference RNAi approach Transgenic plants |
| title | Transgenic rice plants carrying RNA interference constructs of AOS (allene oxide synthase) genes show severe male sterility |
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