Regulation of Osteogenesis and Survival within Bone Grafts to the Calvaria: The Effect of the Dura versus the Pericranium
The present study evaluates the isolated role of dura and pericranium in the survival of fresh (osteoblasts viable) and frozen (osteoblasts nonviable) bone grafts. Bilateral craniectomies were performed in 48 mature rabbits. On one side, bone was replaced immediately; on the contralateral side, it w...
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Veröffentlicht in: | Plastic and reconstructive surgery (1963) 2011-07, Vol.128 (1), p.85-94 |
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creator | Gosain, Arun K. Gosain, Sankalp A. Sweeney, Walter M. Song, Lian-Sheng Amarante, Marco T. J. |
description | The present study evaluates the isolated role of dura and pericranium in the survival of fresh (osteoblasts viable) and frozen (osteoblasts nonviable) bone grafts.
Bilateral craniectomies were performed in 48 mature rabbits. On one side, bone was replaced immediately; on the contralateral side, it was flash-frozen before replacement. Animals were randomized into four groups by placement of Silastic barriers adjacent to bone grafts, as follows: (1) control (no barriers); (2) dural barrier; (3) pericranial barrier; and (4) double (dural and pericranial) barriers. Fluorescein labels were injected at specified intervals, with animals euthanized after 1 or 10 weeks.
After 1 week, fresh grafts without dural barriers demonstrated greater fluorescein labeling on the dural than on the pericranial surface (p < 0.05); in contrast, fresh grafts without pericranial barriers had no statistical difference in fluorescein labeling between pericranial and dural surfaces. After 10 weeks, the new bone area was greater in fresh than in frozen grafts (p < 0.05). Total new bone area and dural-side new bone were greater in grafts without dural barriers (p < 0.001); this was not seen in grafts without pericranial barriers. Pericranial new bone was greatest in fresh grafts without a pericranial barrier (p < 0.001); this was not seen in frozen grafts.
The dura and pericranium each contributed to osteogenesis, although dural contact was more effective. Maintenance of dural contact enhanced osteogenesis through the entire graft, whereas pericranial contact enhanced osteogenesis only on the pericranial surface of fresh grafts. These data suggest dura is largely responsible for cranial graft survival. |
doi_str_mv | 10.1097/PRS.0b013e31821740cc |
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Bilateral craniectomies were performed in 48 mature rabbits. On one side, bone was replaced immediately; on the contralateral side, it was flash-frozen before replacement. Animals were randomized into four groups by placement of Silastic barriers adjacent to bone grafts, as follows: (1) control (no barriers); (2) dural barrier; (3) pericranial barrier; and (4) double (dural and pericranial) barriers. Fluorescein labels were injected at specified intervals, with animals euthanized after 1 or 10 weeks.
After 1 week, fresh grafts without dural barriers demonstrated greater fluorescein labeling on the dural than on the pericranial surface (p < 0.05); in contrast, fresh grafts without pericranial barriers had no statistical difference in fluorescein labeling between pericranial and dural surfaces. After 10 weeks, the new bone area was greater in fresh than in frozen grafts (p < 0.05). Total new bone area and dural-side new bone were greater in grafts without dural barriers (p < 0.001); this was not seen in grafts without pericranial barriers. Pericranial new bone was greatest in fresh grafts without a pericranial barrier (p < 0.001); this was not seen in frozen grafts.
The dura and pericranium each contributed to osteogenesis, although dural contact was more effective. Maintenance of dural contact enhanced osteogenesis through the entire graft, whereas pericranial contact enhanced osteogenesis only on the pericranial surface of fresh grafts. These data suggest dura is largely responsible for cranial graft survival.</description><identifier>ISSN: 0032-1052</identifier><identifier>EISSN: 1529-4242</identifier><identifier>DOI: 10.1097/PRS.0b013e31821740cc</identifier><identifier>PMID: 21399563</identifier><language>eng</language><publisher>Hagerstown, MD: American Society of Plastic Surgeons</publisher><subject>Animals ; Biological and medical sciences ; Bone Regeneration - physiology ; Bone Transplantation ; Cell physiology ; Dura Mater - physiology ; Female ; Fundamental and applied biological sciences. Psychology ; Graft Survival ; Male ; Medical sciences ; Mineralization, calcification ; Molecular and cellular biology ; Osteogenesis ; Rabbits ; Skeleton and joints ; Skull - physiology ; Skull - surgery ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Vertebrates: osteoarticular system, musculoskeletal system</subject><ispartof>Plastic and reconstructive surgery (1963), 2011-07, Vol.128 (1), p.85-94</ispartof><rights>American Society of Plastic Surgeons</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4475-6ad1345eae1f8aa90c63776f8165fddce4691f23d47b8998c248a528f1b86083</citedby><cites>FETCH-LOGICAL-c4475-6ad1345eae1f8aa90c63776f8165fddce4691f23d47b8998c248a528f1b86083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24335482$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21399563$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gosain, Arun K.</creatorcontrib><creatorcontrib>Gosain, Sankalp A.</creatorcontrib><creatorcontrib>Sweeney, Walter M.</creatorcontrib><creatorcontrib>Song, Lian-Sheng</creatorcontrib><creatorcontrib>Amarante, Marco T. J.</creatorcontrib><title>Regulation of Osteogenesis and Survival within Bone Grafts to the Calvaria: The Effect of the Dura versus the Pericranium</title><title>Plastic and reconstructive surgery (1963)</title><addtitle>Plast Reconstr Surg</addtitle><description>The present study evaluates the isolated role of dura and pericranium in the survival of fresh (osteoblasts viable) and frozen (osteoblasts nonviable) bone grafts.
Bilateral craniectomies were performed in 48 mature rabbits. On one side, bone was replaced immediately; on the contralateral side, it was flash-frozen before replacement. Animals were randomized into four groups by placement of Silastic barriers adjacent to bone grafts, as follows: (1) control (no barriers); (2) dural barrier; (3) pericranial barrier; and (4) double (dural and pericranial) barriers. Fluorescein labels were injected at specified intervals, with animals euthanized after 1 or 10 weeks.
After 1 week, fresh grafts without dural barriers demonstrated greater fluorescein labeling on the dural than on the pericranial surface (p < 0.05); in contrast, fresh grafts without pericranial barriers had no statistical difference in fluorescein labeling between pericranial and dural surfaces. After 10 weeks, the new bone area was greater in fresh than in frozen grafts (p < 0.05). Total new bone area and dural-side new bone were greater in grafts without dural barriers (p < 0.001); this was not seen in grafts without pericranial barriers. Pericranial new bone was greatest in fresh grafts without a pericranial barrier (p < 0.001); this was not seen in frozen grafts.
The dura and pericranium each contributed to osteogenesis, although dural contact was more effective. Maintenance of dural contact enhanced osteogenesis through the entire graft, whereas pericranial contact enhanced osteogenesis only on the pericranial surface of fresh grafts. These data suggest dura is largely responsible for cranial graft survival.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Regeneration - physiology</subject><subject>Bone Transplantation</subject><subject>Cell physiology</subject><subject>Dura Mater - physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Graft Survival</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mineralization, calcification</subject><subject>Molecular and cellular biology</subject><subject>Osteogenesis</subject><subject>Rabbits</subject><subject>Skeleton and joints</subject><subject>Skull - physiology</subject><subject>Skull - surgery</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>0032-1052</issn><issn>1529-4242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkF1LHDEUhkNpqVv1H5SSm9Kr0XzOZHpXt2oFQdG9H85mTty02RmbZHbx3zer2woNhPCS55zDeQj5yNkJZ21zent3f8KWjEuU3AjeKGbtGzLjWrSVEkq8JTPGpKg40-KAfEjpJ2O8kbV-Tw4El22razkjT3f4MAXIfhzo6OhNyjg-4IDJJwpDT--nuPEbCHTr88oP9GwckF5GcDnRPNK8QjqHsIHo4StdlHTuHNq867X7-z5FoBuMaUrP-RajtxEGP62PyDsHIeHx_j0ki4vzxfxHdX1zeTX_dl1ZpRpd1dBzqTQCcmcAWmZr2TS1M7zWru8tqrrlTsheNUvTtsYKZUAL4_jS1MzIQ_Llpe1jHH9PmHK39sliCDDgOKXOFHG8aYUupHohbRxTiui6x-jXEJ86zrqd8q4o7_5XXso-7QdMyzX2_4r-Oi7A5z0AyUJwZX3r0yunpNTKiNf52zHkouxXmLYYuxVCyKuOlVNrqSrBOGdNSVW5XMs_kziafQ</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Gosain, Arun K.</creator><creator>Gosain, Sankalp A.</creator><creator>Sweeney, Walter M.</creator><creator>Song, Lian-Sheng</creator><creator>Amarante, Marco T. J.</creator><general>American Society of Plastic Surgeons</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110701</creationdate><title>Regulation of Osteogenesis and Survival within Bone Grafts to the Calvaria: The Effect of the Dura versus the Pericranium</title><author>Gosain, Arun K. ; Gosain, Sankalp A. ; Sweeney, Walter M. ; Song, Lian-Sheng ; Amarante, Marco T. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4475-6ad1345eae1f8aa90c63776f8165fddce4691f23d47b8998c248a528f1b86083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Regeneration - physiology</topic><topic>Bone Transplantation</topic><topic>Cell physiology</topic><topic>Dura Mater - physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Graft Survival</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mineralization, calcification</topic><topic>Molecular and cellular biology</topic><topic>Osteogenesis</topic><topic>Rabbits</topic><topic>Skeleton and joints</topic><topic>Skull - physiology</topic><topic>Skull - surgery</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gosain, Arun K.</creatorcontrib><creatorcontrib>Gosain, Sankalp A.</creatorcontrib><creatorcontrib>Sweeney, Walter M.</creatorcontrib><creatorcontrib>Song, Lian-Sheng</creatorcontrib><creatorcontrib>Amarante, Marco T. J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plastic and reconstructive surgery (1963)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gosain, Arun K.</au><au>Gosain, Sankalp A.</au><au>Sweeney, Walter M.</au><au>Song, Lian-Sheng</au><au>Amarante, Marco T. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Regulation of Osteogenesis and Survival within Bone Grafts to the Calvaria: The Effect of the Dura versus the Pericranium</atitle><jtitle>Plastic and reconstructive surgery (1963)</jtitle><addtitle>Plast Reconstr Surg</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>128</volume><issue>1</issue><spage>85</spage><epage>94</epage><pages>85-94</pages><issn>0032-1052</issn><eissn>1529-4242</eissn><abstract>The present study evaluates the isolated role of dura and pericranium in the survival of fresh (osteoblasts viable) and frozen (osteoblasts nonviable) bone grafts.
Bilateral craniectomies were performed in 48 mature rabbits. On one side, bone was replaced immediately; on the contralateral side, it was flash-frozen before replacement. Animals were randomized into four groups by placement of Silastic barriers adjacent to bone grafts, as follows: (1) control (no barriers); (2) dural barrier; (3) pericranial barrier; and (4) double (dural and pericranial) barriers. Fluorescein labels were injected at specified intervals, with animals euthanized after 1 or 10 weeks.
After 1 week, fresh grafts without dural barriers demonstrated greater fluorescein labeling on the dural than on the pericranial surface (p < 0.05); in contrast, fresh grafts without pericranial barriers had no statistical difference in fluorescein labeling between pericranial and dural surfaces. After 10 weeks, the new bone area was greater in fresh than in frozen grafts (p < 0.05). Total new bone area and dural-side new bone were greater in grafts without dural barriers (p < 0.001); this was not seen in grafts without pericranial barriers. Pericranial new bone was greatest in fresh grafts without a pericranial barrier (p < 0.001); this was not seen in frozen grafts.
The dura and pericranium each contributed to osteogenesis, although dural contact was more effective. Maintenance of dural contact enhanced osteogenesis through the entire graft, whereas pericranial contact enhanced osteogenesis only on the pericranial surface of fresh grafts. These data suggest dura is largely responsible for cranial graft survival.</abstract><cop>Hagerstown, MD</cop><pub>American Society of Plastic Surgeons</pub><pmid>21399563</pmid><doi>10.1097/PRS.0b013e31821740cc</doi><tpages>10</tpages></addata></record> |
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subjects | Animals Biological and medical sciences Bone Regeneration - physiology Bone Transplantation Cell physiology Dura Mater - physiology Female Fundamental and applied biological sciences. Psychology Graft Survival Male Medical sciences Mineralization, calcification Molecular and cellular biology Osteogenesis Rabbits Skeleton and joints Skull - physiology Skull - surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Vertebrates: osteoarticular system, musculoskeletal system |
title | Regulation of Osteogenesis and Survival within Bone Grafts to the Calvaria: The Effect of the Dura versus the Pericranium |
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