Virally induced changes in cellular microRNAs maintain latency of human cytomegalovirus in CD34+ progenitors
One site of latency of human cytomegalovirus (HCMV; human herpesvirus 5) is known to be CD34(+) haematopoietic progenitor cells, and it is likely that carriage of latent virus has profound effects on cellular gene expression in order to optimize latency and reactivation. As microRNAs (miRNAs) play i...
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| Veröffentlicht in: | Journal of general virology 2011-07, Vol.92 (Pt 7), p.1539-1549 |
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| container_issue | Pt 7 |
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| container_title | Journal of general virology |
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| creator | POOLE, Emma MCGREGOR DALLAS, Stuart R COLSTON, Julia JOSEPH, Robert Samuel V SINCLAIR, John |
| description | One site of latency of human cytomegalovirus (HCMV; human herpesvirus 5) is known to be CD34(+) haematopoietic progenitor cells, and it is likely that carriage of latent virus has profound effects on cellular gene expression in order to optimize latency and reactivation. As microRNAs (miRNAs) play important roles in regulating stem-cell gene expression, this study asked whether latent carriage of HCMV led to changes in cellular miRNA expression. A comprehensive miRNA screen showed the differential regulation of a number of cellular miRNAs during HCMV latency in CD34(+) progenitor cells. One of these, hsa-miR-92a, was robustly decreased in three independent miRNA screens. Latency-induced change in hsa-miR-92a results in an increase in expression of GATA-2 and subsequent increased expression of cellular IL-10, which aids the maintenance of latent viral genomes in CD34(+) cells, probably resulting from their increased survival. |
| doi_str_mv | 10.1099/vir.0.031377-0 |
| format | Article |
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As microRNAs (miRNAs) play important roles in regulating stem-cell gene expression, this study asked whether latent carriage of HCMV led to changes in cellular miRNA expression. A comprehensive miRNA screen showed the differential regulation of a number of cellular miRNAs during HCMV latency in CD34(+) progenitor cells. One of these, hsa-miR-92a, was robustly decreased in three independent miRNA screens. Latency-induced change in hsa-miR-92a results in an increase in expression of GATA-2 and subsequent increased expression of cellular IL-10, which aids the maintenance of latent viral genomes in CD34(+) cells, probably resulting from their increased survival.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/vir.0.031377-0</identifier><identifier>PMID: 21471310</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Society for General Microbiology</publisher><subject>Antigens, CD34 - genetics ; Antigens, CD34 - metabolism ; Biological and medical sciences ; Cell Line ; Cytomegalovirus - genetics ; Cytomegalovirus - physiology ; Cytomegalovirus Infections - genetics ; Cytomegalovirus Infections - metabolism ; Cytomegalovirus Infections - virology ; Fundamental and applied biological sciences. 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Psychology</subject><subject>Gene Expression Regulation</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Hematopoietic Stem Cells - virology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Miscellaneous</subject><subject>Virology</subject><subject>Virus Latency</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1P3DAQxa2qqCwL1x4rX6oeUJbxR-zsES3lQ0IgIeAaTRx7ceUk1E6Q9r_HsAscRqOxf37j9wj5yWDBYLk8efFxAQsQTGhdwDcyY1KVBc9X38kMgPOCCab3yUFK_wCYlKX-QfY5kzqfw4yERx8xhA31fTsZ21LzhP3apjxTY0OYAkbaeROHu5vTRDv0_ZiLBhxtbzZ0cPRp6jDDm3Ho7BrDkL80vb9fnQl5TJ_jsLa9H4eYDsmew5Ds0a7PycP53_vVZXF9e3G1Or0ujFByLNBUDo3lLbhWNWBasVQSjVaNKDF7MMqgKttKOqcdb5olWHBOVUzZ0timEnPyZ6ubd_-fbBrrzqc3N9jbYUp1pQXjHBjP5GJLZoMpRevq5-g7jJuaQf0WcJ3d1FBvA85tTn7tpKems-0n_pFoBn7vAEwGg4vYG5--OMmrvFeLV7GJhfc</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>POOLE, Emma</creator><creator>MCGREGOR DALLAS, Stuart R</creator><creator>COLSTON, Julia</creator><creator>JOSEPH, Robert Samuel V</creator><creator>SINCLAIR, John</creator><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110701</creationdate><title>Virally induced changes in cellular microRNAs maintain latency of human cytomegalovirus in CD34+ progenitors</title><author>POOLE, Emma ; MCGREGOR DALLAS, Stuart R ; COLSTON, Julia ; JOSEPH, Robert Samuel V ; SINCLAIR, John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-ac8face2d0fd6b0cd3964ac76b35a014c6ca65d84ff7f2bb90e0ff6816e5ceb83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Antigens, CD34 - genetics</topic><topic>Antigens, CD34 - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cytomegalovirus - genetics</topic><topic>Cytomegalovirus - physiology</topic><topic>Cytomegalovirus Infections - genetics</topic><topic>Cytomegalovirus Infections - metabolism</topic><topic>Cytomegalovirus Infections - virology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Hematopoietic Stem Cells - virology</topic><topic>Humans</topic><topic>Microbiology</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Miscellaneous</topic><topic>Virology</topic><topic>Virus Latency</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>POOLE, Emma</creatorcontrib><creatorcontrib>MCGREGOR DALLAS, Stuart R</creatorcontrib><creatorcontrib>COLSTON, Julia</creatorcontrib><creatorcontrib>JOSEPH, Robert Samuel V</creatorcontrib><creatorcontrib>SINCLAIR, John</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>POOLE, Emma</au><au>MCGREGOR DALLAS, Stuart R</au><au>COLSTON, Julia</au><au>JOSEPH, Robert Samuel V</au><au>SINCLAIR, John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Virally induced changes in cellular microRNAs maintain latency of human cytomegalovirus in CD34+ progenitors</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>92</volume><issue>Pt 7</issue><spage>1539</spage><epage>1549</epage><pages>1539-1549</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>One site of latency of human cytomegalovirus (HCMV; human herpesvirus 5) is known to be CD34(+) haematopoietic progenitor cells, and it is likely that carriage of latent virus has profound effects on cellular gene expression in order to optimize latency and reactivation. 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| ispartof | Journal of general virology, 2011-07, Vol.92 (Pt 7), p.1539-1549 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_873122012 |
| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Antigens, CD34 - genetics Antigens, CD34 - metabolism Biological and medical sciences Cell Line Cytomegalovirus - genetics Cytomegalovirus - physiology Cytomegalovirus Infections - genetics Cytomegalovirus Infections - metabolism Cytomegalovirus Infections - virology Fundamental and applied biological sciences. Psychology Gene Expression Regulation Hematopoietic Stem Cells - metabolism Hematopoietic Stem Cells - virology Humans Microbiology MicroRNAs - genetics MicroRNAs - metabolism Miscellaneous Virology Virus Latency |
| title | Virally induced changes in cellular microRNAs maintain latency of human cytomegalovirus in CD34+ progenitors |
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