Intracellular signaling cascades induced by relaxin in the stimulation of capacitation and acrosome reaction in fresh and frozen-thawed bovine spermatozoa
Relaxin is one of the 6-kDa peptide hormones, which acts as a pleiotropic endocrine and paracrine factor. Our previous studies revealed that sperm capacitating medium containing relaxin induced capacitation and acrosome reaction (AR) in fresh and frozen-thawed porcine or bovine spermatozoa. However,...
Gespeichert in:
| Veröffentlicht in: | Animal reproduction science 2011-05, Vol.125 (1-4), p.30-41 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 41 |
|---|---|
| container_issue | 1-4 |
| container_start_page | 30 |
| container_title | Animal reproduction science |
| container_volume | 125 |
| creator | Miah, A.G Salma, U Sinha, P.B Hölker, M Tesfaye, D Cinar, M.U Tsujii, H Schellander, K |
| description | Relaxin is one of the 6-kDa peptide hormones, which acts as a pleiotropic endocrine and paracrine factor. Our previous studies revealed that sperm capacitating medium containing relaxin induced capacitation and acrosome reaction (AR) in fresh and frozen-thawed porcine or bovine spermatozoa. However, the intracellular signaling cascades involved with capacitation or AR induced by relaxin was unknown. Therefore, the present study was designed to investigate the intracellular signaling cascades involved with capacitation and AR induced by relaxin in fresh and frozen-thawed bovine spermatozoa. Spermatozoa were incubated in sperm Tyrode's albumin lactate pyruvate (Sp-TALP) medium supplemented with (40ngml⁻¹) or without relaxin, and subjected to evaluation of chlortetracycline staining pattern, cholesterol efflux, Ca²⁺-influx, intracellular cyclic adenosine monophosphate (cAMP) and protein tyrosine phosphorylation. Capacitation and AR were increased (P |
| doi_str_mv | 10.1016/j.anireprosci.2011.03.010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_872527448</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378432011000893</els_id><sourcerecordid>872527448</sourcerecordid><originalsourceid>FETCH-LOGICAL-c424t-6a28077255559ce2a02b5cdffd44a15067a6731fa4ad1038d9e8d32bede01c963</originalsourceid><addsrcrecordid>eNqNUctuFDEQHCEQ2QR-AYYTp1n82nkc0QpIpEgcIGer1-7Z9WrGXmxPIPkUvpaeTECcEL5YbldVV3cVxRvO1pzx-t1xDd5FPMWQjFsLxvmayTXj7Emx4m0jKyGkeFqsmGzaSknBzorzlI6Msaauu-fFmeCqk4x3q-Lnlc8RDA7DNEAsk9t7GJzflwaSAYupdN5OBm25uysjDvDDeSqV-YBlym4kVnbBl6EnxgmMy8sbvC3BkMEwIvHAPFSJ2EdMh4fvPoZ79FU-wPdZPtw6T5onjCPkcB_gRfGshyHhy8f7orj5-OHr9rK6_vzpavv-ujJKqFzVIFrWNGJDpzMogIndxti-t0oB37C6gbqRvAcFljPZ2g5bK8UOLTJuulpeFG8XXdrntwlT1qNL80bAY5iSbklbNEq1hOwW5DxYitjrU3QjxDvNmZ6T0Uf9VzJ6TkYzqSkZ4r567DLtRrR_mL-jIMDrBdBD0LCPLumbL6SgKDapeN3-E8HrRs1NtgsCaV-3DqMmG-gpPjJlsrbB_YfVX0R4u-w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>872527448</pqid></control><display><type>article</type><title>Intracellular signaling cascades induced by relaxin in the stimulation of capacitation and acrosome reaction in fresh and frozen-thawed bovine spermatozoa</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Miah, A.G ; Salma, U ; Sinha, P.B ; Hölker, M ; Tesfaye, D ; Cinar, M.U ; Tsujii, H ; Schellander, K</creator><creatorcontrib>Miah, A.G ; Salma, U ; Sinha, P.B ; Hölker, M ; Tesfaye, D ; Cinar, M.U ; Tsujii, H ; Schellander, K</creatorcontrib><description><![CDATA[Relaxin is one of the 6-kDa peptide hormones, which acts as a pleiotropic endocrine and paracrine factor. Our previous studies revealed that sperm capacitating medium containing relaxin induced capacitation and acrosome reaction (AR) in fresh and frozen-thawed porcine or bovine spermatozoa. However, the intracellular signaling cascades involved with capacitation or AR induced by relaxin was unknown. Therefore, the present study was designed to investigate the intracellular signaling cascades involved with capacitation and AR induced by relaxin in fresh and frozen-thawed bovine spermatozoa. Spermatozoa were incubated in sperm Tyrode's albumin lactate pyruvate (Sp-TALP) medium supplemented with (40ngml⁻¹) or without relaxin, and subjected to evaluation of chlortetracycline staining pattern, cholesterol efflux, Ca²⁺-influx, intracellular cyclic adenosine monophosphate (cAMP) and protein tyrosine phosphorylation. Capacitation and AR were increased (P <0.05) in both fresh and frozen-thawed spermatozoa incubated with relaxin. Cholesterol effluxes were greater in the fresh (P <0.01) and frozen-thawed (P <0.05) spermatozoa incubated with relaxin than the spermatozoa incubated without relaxin. Ca²⁺-influxes were also significantly stimulated by relaxin in the fresh (P <0.01) and frozen-thawed (P <0.05) spermatozoa. The Sp-TALP medium containing relaxin influenced the generation of intracellular cAMP in the fresh (P <0.01) and frozen-thawed (P <0.05) spermatozoa, and exhibited higher exposure of protein tyrosine phosphorylation in both sperm types than the medium devoid of relaxin. Therefore, the results postulate that relaxin exerts the intracellular signaling cascades involved with capacitation and AR through accelerating the cholesterol efflux, Ca²⁺-influx, intracellular cAMP and protein tyrosine phosphorylation in fresh and frozen-thawed bovine spermatozoa.]]></description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2011.03.010</identifier><identifier>PMID: 21493019</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>acrosome reaction ; Acrosome Reaction - drug effects ; Acrosome Reaction - physiology ; albumins ; Animals ; Ca 2+-influx ; Calcium - physiology ; Capacitation and AR ; cattle ; Cattle - physiology ; Cell Communication - drug effects ; Cell Communication - physiology ; Chlortetracycline - chemistry ; cholesterol ; Cholesterol - physiology ; Cholesterol efflux ; cyclic AMP ; Cyclic AMP - physiology ; Intracellular cAMP ; Male ; Microscopy, Fluorescence - veterinary ; Microscopy, Interference - veterinary ; peptide hormones ; phosphorylation ; Protein tyrosine phosphorylation ; Relaxin ; Relaxin - pharmacology ; Signal Transduction - drug effects ; Signal Transduction - physiology ; sperm capacitation ; Sperm Capacitation - drug effects ; Sperm Capacitation - physiology ; spermatozoa ; Spermatozoa - drug effects ; Spermatozoa - physiology ; tyrosine</subject><ispartof>Animal reproduction science, 2011-05, Vol.125 (1-4), p.30-41</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-6a28077255559ce2a02b5cdffd44a15067a6731fa4ad1038d9e8d32bede01c963</citedby><cites>FETCH-LOGICAL-c424t-6a28077255559ce2a02b5cdffd44a15067a6731fa4ad1038d9e8d32bede01c963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.anireprosci.2011.03.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21493019$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miah, A.G</creatorcontrib><creatorcontrib>Salma, U</creatorcontrib><creatorcontrib>Sinha, P.B</creatorcontrib><creatorcontrib>Hölker, M</creatorcontrib><creatorcontrib>Tesfaye, D</creatorcontrib><creatorcontrib>Cinar, M.U</creatorcontrib><creatorcontrib>Tsujii, H</creatorcontrib><creatorcontrib>Schellander, K</creatorcontrib><title>Intracellular signaling cascades induced by relaxin in the stimulation of capacitation and acrosome reaction in fresh and frozen-thawed bovine spermatozoa</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description><![CDATA[Relaxin is one of the 6-kDa peptide hormones, which acts as a pleiotropic endocrine and paracrine factor. Our previous studies revealed that sperm capacitating medium containing relaxin induced capacitation and acrosome reaction (AR) in fresh and frozen-thawed porcine or bovine spermatozoa. However, the intracellular signaling cascades involved with capacitation or AR induced by relaxin was unknown. Therefore, the present study was designed to investigate the intracellular signaling cascades involved with capacitation and AR induced by relaxin in fresh and frozen-thawed bovine spermatozoa. Spermatozoa were incubated in sperm Tyrode's albumin lactate pyruvate (Sp-TALP) medium supplemented with (40ngml⁻¹) or without relaxin, and subjected to evaluation of chlortetracycline staining pattern, cholesterol efflux, Ca²⁺-influx, intracellular cyclic adenosine monophosphate (cAMP) and protein tyrosine phosphorylation. Capacitation and AR were increased (P <0.05) in both fresh and frozen-thawed spermatozoa incubated with relaxin. Cholesterol effluxes were greater in the fresh (P <0.01) and frozen-thawed (P <0.05) spermatozoa incubated with relaxin than the spermatozoa incubated without relaxin. Ca²⁺-influxes were also significantly stimulated by relaxin in the fresh (P <0.01) and frozen-thawed (P <0.05) spermatozoa. The Sp-TALP medium containing relaxin influenced the generation of intracellular cAMP in the fresh (P <0.01) and frozen-thawed (P <0.05) spermatozoa, and exhibited higher exposure of protein tyrosine phosphorylation in both sperm types than the medium devoid of relaxin. Therefore, the results postulate that relaxin exerts the intracellular signaling cascades involved with capacitation and AR through accelerating the cholesterol efflux, Ca²⁺-influx, intracellular cAMP and protein tyrosine phosphorylation in fresh and frozen-thawed bovine spermatozoa.]]></description><subject>acrosome reaction</subject><subject>Acrosome Reaction - drug effects</subject><subject>Acrosome Reaction - physiology</subject><subject>albumins</subject><subject>Animals</subject><subject>Ca 2+-influx</subject><subject>Calcium - physiology</subject><subject>Capacitation and AR</subject><subject>cattle</subject><subject>Cattle - physiology</subject><subject>Cell Communication - drug effects</subject><subject>Cell Communication - physiology</subject><subject>Chlortetracycline - chemistry</subject><subject>cholesterol</subject><subject>Cholesterol - physiology</subject><subject>Cholesterol efflux</subject><subject>cyclic AMP</subject><subject>Cyclic AMP - physiology</subject><subject>Intracellular cAMP</subject><subject>Male</subject><subject>Microscopy, Fluorescence - veterinary</subject><subject>Microscopy, Interference - veterinary</subject><subject>peptide hormones</subject><subject>phosphorylation</subject><subject>Protein tyrosine phosphorylation</subject><subject>Relaxin</subject><subject>Relaxin - pharmacology</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - physiology</subject><subject>sperm capacitation</subject><subject>Sperm Capacitation - drug effects</subject><subject>Sperm Capacitation - physiology</subject><subject>spermatozoa</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>tyrosine</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUctuFDEQHCEQ2QR-AYYTp1n82nkc0QpIpEgcIGer1-7Z9WrGXmxPIPkUvpaeTECcEL5YbldVV3cVxRvO1pzx-t1xDd5FPMWQjFsLxvmayTXj7Emx4m0jKyGkeFqsmGzaSknBzorzlI6Msaauu-fFmeCqk4x3q-Lnlc8RDA7DNEAsk9t7GJzflwaSAYupdN5OBm25uysjDvDDeSqV-YBlym4kVnbBl6EnxgmMy8sbvC3BkMEwIvHAPFSJ2EdMh4fvPoZ79FU-wPdZPtw6T5onjCPkcB_gRfGshyHhy8f7orj5-OHr9rK6_vzpavv-ujJKqFzVIFrWNGJDpzMogIndxti-t0oB37C6gbqRvAcFljPZ2g5bK8UOLTJuulpeFG8XXdrntwlT1qNL80bAY5iSbklbNEq1hOwW5DxYitjrU3QjxDvNmZ6T0Uf9VzJ6TkYzqSkZ4r567DLtRrR_mL-jIMDrBdBD0LCPLumbL6SgKDapeN3-E8HrRs1NtgsCaV-3DqMmG-gpPjJlsrbB_YfVX0R4u-w</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>Miah, A.G</creator><creator>Salma, U</creator><creator>Sinha, P.B</creator><creator>Hölker, M</creator><creator>Tesfaye, D</creator><creator>Cinar, M.U</creator><creator>Tsujii, H</creator><creator>Schellander, K</creator><general>Elsevier B.V</general><general>[Amsterdam]: Elsevier Science</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110501</creationdate><title>Intracellular signaling cascades induced by relaxin in the stimulation of capacitation and acrosome reaction in fresh and frozen-thawed bovine spermatozoa</title><author>Miah, A.G ; Salma, U ; Sinha, P.B ; Hölker, M ; Tesfaye, D ; Cinar, M.U ; Tsujii, H ; Schellander, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-6a28077255559ce2a02b5cdffd44a15067a6731fa4ad1038d9e8d32bede01c963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>acrosome reaction</topic><topic>Acrosome Reaction - drug effects</topic><topic>Acrosome Reaction - physiology</topic><topic>albumins</topic><topic>Animals</topic><topic>Ca 2+-influx</topic><topic>Calcium - physiology</topic><topic>Capacitation and AR</topic><topic>cattle</topic><topic>Cattle - physiology</topic><topic>Cell Communication - drug effects</topic><topic>Cell Communication - physiology</topic><topic>Chlortetracycline - chemistry</topic><topic>cholesterol</topic><topic>Cholesterol - physiology</topic><topic>Cholesterol efflux</topic><topic>cyclic AMP</topic><topic>Cyclic AMP - physiology</topic><topic>Intracellular cAMP</topic><topic>Male</topic><topic>Microscopy, Fluorescence - veterinary</topic><topic>Microscopy, Interference - veterinary</topic><topic>peptide hormones</topic><topic>phosphorylation</topic><topic>Protein tyrosine phosphorylation</topic><topic>Relaxin</topic><topic>Relaxin - pharmacology</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - physiology</topic><topic>sperm capacitation</topic><topic>Sperm Capacitation - drug effects</topic><topic>Sperm Capacitation - physiology</topic><topic>spermatozoa</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>tyrosine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Miah, A.G</creatorcontrib><creatorcontrib>Salma, U</creatorcontrib><creatorcontrib>Sinha, P.B</creatorcontrib><creatorcontrib>Hölker, M</creatorcontrib><creatorcontrib>Tesfaye, D</creatorcontrib><creatorcontrib>Cinar, M.U</creatorcontrib><creatorcontrib>Tsujii, H</creatorcontrib><creatorcontrib>Schellander, K</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Miah, A.G</au><au>Salma, U</au><au>Sinha, P.B</au><au>Hölker, M</au><au>Tesfaye, D</au><au>Cinar, M.U</au><au>Tsujii, H</au><au>Schellander, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intracellular signaling cascades induced by relaxin in the stimulation of capacitation and acrosome reaction in fresh and frozen-thawed bovine spermatozoa</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2011-05-01</date><risdate>2011</risdate><volume>125</volume><issue>1-4</issue><spage>30</spage><epage>41</epage><pages>30-41</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract><![CDATA[Relaxin is one of the 6-kDa peptide hormones, which acts as a pleiotropic endocrine and paracrine factor. Our previous studies revealed that sperm capacitating medium containing relaxin induced capacitation and acrosome reaction (AR) in fresh and frozen-thawed porcine or bovine spermatozoa. However, the intracellular signaling cascades involved with capacitation or AR induced by relaxin was unknown. Therefore, the present study was designed to investigate the intracellular signaling cascades involved with capacitation and AR induced by relaxin in fresh and frozen-thawed bovine spermatozoa. Spermatozoa were incubated in sperm Tyrode's albumin lactate pyruvate (Sp-TALP) medium supplemented with (40ngml⁻¹) or without relaxin, and subjected to evaluation of chlortetracycline staining pattern, cholesterol efflux, Ca²⁺-influx, intracellular cyclic adenosine monophosphate (cAMP) and protein tyrosine phosphorylation. Capacitation and AR were increased (P <0.05) in both fresh and frozen-thawed spermatozoa incubated with relaxin. Cholesterol effluxes were greater in the fresh (P <0.01) and frozen-thawed (P <0.05) spermatozoa incubated with relaxin than the spermatozoa incubated without relaxin. Ca²⁺-influxes were also significantly stimulated by relaxin in the fresh (P <0.01) and frozen-thawed (P <0.05) spermatozoa. The Sp-TALP medium containing relaxin influenced the generation of intracellular cAMP in the fresh (P <0.01) and frozen-thawed (P <0.05) spermatozoa, and exhibited higher exposure of protein tyrosine phosphorylation in both sperm types than the medium devoid of relaxin. Therefore, the results postulate that relaxin exerts the intracellular signaling cascades involved with capacitation and AR through accelerating the cholesterol efflux, Ca²⁺-influx, intracellular cAMP and protein tyrosine phosphorylation in fresh and frozen-thawed bovine spermatozoa.]]></abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21493019</pmid><doi>10.1016/j.anireprosci.2011.03.010</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-4320 |
| ispartof | Animal reproduction science, 2011-05, Vol.125 (1-4), p.30-41 |
| issn | 0378-4320 1873-2232 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_872527448 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | acrosome reaction Acrosome Reaction - drug effects Acrosome Reaction - physiology albumins Animals Ca 2+-influx Calcium - physiology Capacitation and AR cattle Cattle - physiology Cell Communication - drug effects Cell Communication - physiology Chlortetracycline - chemistry cholesterol Cholesterol - physiology Cholesterol efflux cyclic AMP Cyclic AMP - physiology Intracellular cAMP Male Microscopy, Fluorescence - veterinary Microscopy, Interference - veterinary peptide hormones phosphorylation Protein tyrosine phosphorylation Relaxin Relaxin - pharmacology Signal Transduction - drug effects Signal Transduction - physiology sperm capacitation Sperm Capacitation - drug effects Sperm Capacitation - physiology spermatozoa Spermatozoa - drug effects Spermatozoa - physiology tyrosine |
| title | Intracellular signaling cascades induced by relaxin in the stimulation of capacitation and acrosome reaction in fresh and frozen-thawed bovine spermatozoa |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T01%3A16%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Intracellular%20signaling%20cascades%20induced%20by%20relaxin%20in%20the%20stimulation%20of%20capacitation%20and%20acrosome%20reaction%20in%20fresh%20and%20frozen-thawed%20bovine%20spermatozoa&rft.jtitle=Animal%20reproduction%20science&rft.au=Miah,%20A.G&rft.date=2011-05-01&rft.volume=125&rft.issue=1-4&rft.spage=30&rft.epage=41&rft.pages=30-41&rft.issn=0378-4320&rft.eissn=1873-2232&rft_id=info:doi/10.1016/j.anireprosci.2011.03.010&rft_dat=%3Cproquest_cross%3E872527448%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=872527448&rft_id=info:pmid/21493019&rft_els_id=S0378432011000893&rfr_iscdi=true |