Optimal concentrations of cryoprotective agents for semen from stallions that are classified ‘good’ or ‘poor’ for freezing

Cryopreserved stallion sperm displays a high degree of male-to-male variability with respect to cell viability after thawing. Animals that have semen with low viability after cryopreservation are classified as ‘poor' freezers, and when post-thaw viability is high they are designated as ‘good�...

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Veröffentlicht in:Animal reproduction science 2011-05, Vol.125 (1-4), p.112-118
Hauptverfasser: Hoffmann, N, Oldenhof, H, Morandini, C, Rohn, K, Sieme, H
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container_end_page 118
container_issue 1-4
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container_title Animal reproduction science
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creator Hoffmann, N
Oldenhof, H
Morandini, C
Rohn, K
Sieme, H
description Cryopreserved stallion sperm displays a high degree of male-to-male variability with respect to cell viability after thawing. Animals that have semen with low viability after cryopreservation are classified as ‘poor' freezers, and when post-thaw viability is high they are designated as ‘good' freezers. Cryoprotective agents that are used for cryopreserving stallion sperm include glycerol, ethylene glycol, methyl formamide, and dimethylformamide, and are typically used in concentrations ranging from 1% to 4%. The aim of this study was to evaluate the osmotic stresses that stallion sperm is exposed to during cryopreservation, and to determine if sperm from ‘good' and ‘poor' freezers show differences in osmotic tolerance limits and in the suitability of cryoprotective agents. Concentrations of 2-3% of the above mentioned cryoprotectants with freezing extender osmolalities ranging from 580 to 895mOsmkg⁻¹ showed the highest motility rates after freeze-thaw, both for ‘good' and ‘poor' freezers, for all cryoprotectants tested with slightly higher values for glycerol. Freeze-thawed semen from ‘poor' freezers was found to have a lower percentage of progressively motile sperm compared to that of ‘good' freezers. Assessment of plasma and acrosomal membrane integrity after return to isosmotic conditions revealed that cryopreserved sperm from ‘poor' freezers showed lower osmotic tolerance limits as compared to sperm from ‘good' freezers. Semen from ‘poor' freezers that was frozen using freezing extenders supplemented with more then 2% cryoprotectant showed decreased viability and increased acrosome reaction upon return to isoosmotic conditions, whereas ‘good' freezers could withstand cryoprotectant concentrations up to 3% before a decline in viability was observed.
doi_str_mv 10.1016/j.anireprosci.2011.03.001
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subjects acrosome reaction
Acrosome Reaction - physiology
Animals
Cell Survival - drug effects
cell viability
Cryopreservation
Cryopreservation - methods
Cryopreservation - veterinary
Cryoprotectant
cryoprotectants
Cryoprotective Agents
ethylene glycol
Flow Cytometry - veterinary
freezers
freezing
glycerol
Horses
Male
Male heterogeneity
Osmolar Concentration
osmotic stress
Osmotic tolerance
osmotolerance
Semen
Semen Preservation - methods
Semen Preservation - veterinary
Sperm Motility - physiology
spermatozoa
Stallion semen
stallions
thawing
title Optimal concentrations of cryoprotective agents for semen from stallions that are classified ‘good’ or ‘poor’ for freezing
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