Optimal concentrations of cryoprotective agents for semen from stallions that are classified ‘good’ or ‘poor’ for freezing
Cryopreserved stallion sperm displays a high degree of male-to-male variability with respect to cell viability after thawing. Animals that have semen with low viability after cryopreservation are classified as ‘poor' freezers, and when post-thaw viability is high they are designated as ‘good...
Gespeichert in:
Veröffentlicht in: | Animal reproduction science 2011-05, Vol.125 (1-4), p.112-118 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 118 |
---|---|
container_issue | 1-4 |
container_start_page | 112 |
container_title | Animal reproduction science |
container_volume | 125 |
creator | Hoffmann, N Oldenhof, H Morandini, C Rohn, K Sieme, H |
description | Cryopreserved stallion sperm displays a high degree of male-to-male variability with respect to cell viability after thawing. Animals that have semen with low viability after cryopreservation are classified as ‘poor' freezers, and when post-thaw viability is high they are designated as ‘good' freezers. Cryoprotective agents that are used for cryopreserving stallion sperm include glycerol, ethylene glycol, methyl formamide, and dimethylformamide, and are typically used in concentrations ranging from 1% to 4%. The aim of this study was to evaluate the osmotic stresses that stallion sperm is exposed to during cryopreservation, and to determine if sperm from ‘good' and ‘poor' freezers show differences in osmotic tolerance limits and in the suitability of cryoprotective agents. Concentrations of 2-3% of the above mentioned cryoprotectants with freezing extender osmolalities ranging from 580 to 895mOsmkg⁻¹ showed the highest motility rates after freeze-thaw, both for ‘good' and ‘poor' freezers, for all cryoprotectants tested with slightly higher values for glycerol. Freeze-thawed semen from ‘poor' freezers was found to have a lower percentage of progressively motile sperm compared to that of ‘good' freezers. Assessment of plasma and acrosomal membrane integrity after return to isosmotic conditions revealed that cryopreserved sperm from ‘poor' freezers showed lower osmotic tolerance limits as compared to sperm from ‘good' freezers. Semen from ‘poor' freezers that was frozen using freezing extenders supplemented with more then 2% cryoprotectant showed decreased viability and increased acrosome reaction upon return to isoosmotic conditions, whereas ‘good' freezers could withstand cryoprotectant concentrations up to 3% before a decline in viability was observed. |
doi_str_mv | 10.1016/j.anireprosci.2011.03.001 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_872527428</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378432011000716</els_id><sourcerecordid>872527428</sourcerecordid><originalsourceid>FETCH-LOGICAL-c480t-88199c3882ca21f333fe6257fcc6222e84c9565a66d2bf34e2cabd0734c029863</originalsourceid><addsrcrecordid>eNqNkc1u1DAUhS0EokPhFcCsukrqn8RxlmhEC1KlLkrXlse5HjxK4sH2VGpXFU8Br9cn6R2mIFaIlXXl71wfn0PIe85qzrg63dR2Dgm2KWYXasE4r5msGePPyILrTlZCSPGcLJjsdNVIwY7Iq5w3jLFOqf4lORK86ZhmYkG-X25LmOxIXZwdzCXZEuKcafTUpduITxRwJdwAtWu8ztTHRDNMMFOf4kRzseP4S1G-2kJtAupGm3PwAQb6cP9jHePwcP-TogynbYxpP-23-ARwF-b1a_LC2zHDm6fzmFyfffyy_FRdXJ5_Xn64qFyjWam05n3vpNbCWcG9lNKDEm3nnVNCCNCN61vVWqUGsfKyAeRWA-tk45jotZLH5OSwFz_1bQe5mClkB-NoZ4i7bHQnWtE1QiPZH0iHCecE3mwThpRuDWdm34DZmL8aMPsGDJMGG0Dt26dXdqsJhj_K35Ej8O4AeBuNXaeQzfUVbmiwHtnwVv6T4ApdIrE8EIB53QRIBm0AFjigKVfMEMN_WH0EMGC1zA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>872527428</pqid></control><display><type>article</type><title>Optimal concentrations of cryoprotective agents for semen from stallions that are classified ‘good’ or ‘poor’ for freezing</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Hoffmann, N ; Oldenhof, H ; Morandini, C ; Rohn, K ; Sieme, H</creator><creatorcontrib>Hoffmann, N ; Oldenhof, H ; Morandini, C ; Rohn, K ; Sieme, H</creatorcontrib><description>Cryopreserved stallion sperm displays a high degree of male-to-male variability with respect to cell viability after thawing. Animals that have semen with low viability after cryopreservation are classified as ‘poor' freezers, and when post-thaw viability is high they are designated as ‘good' freezers. Cryoprotective agents that are used for cryopreserving stallion sperm include glycerol, ethylene glycol, methyl formamide, and dimethylformamide, and are typically used in concentrations ranging from 1% to 4%. The aim of this study was to evaluate the osmotic stresses that stallion sperm is exposed to during cryopreservation, and to determine if sperm from ‘good' and ‘poor' freezers show differences in osmotic tolerance limits and in the suitability of cryoprotective agents. Concentrations of 2-3% of the above mentioned cryoprotectants with freezing extender osmolalities ranging from 580 to 895mOsmkg⁻¹ showed the highest motility rates after freeze-thaw, both for ‘good' and ‘poor' freezers, for all cryoprotectants tested with slightly higher values for glycerol. Freeze-thawed semen from ‘poor' freezers was found to have a lower percentage of progressively motile sperm compared to that of ‘good' freezers. Assessment of plasma and acrosomal membrane integrity after return to isosmotic conditions revealed that cryopreserved sperm from ‘poor' freezers showed lower osmotic tolerance limits as compared to sperm from ‘good' freezers. Semen from ‘poor' freezers that was frozen using freezing extenders supplemented with more then 2% cryoprotectant showed decreased viability and increased acrosome reaction upon return to isoosmotic conditions, whereas ‘good' freezers could withstand cryoprotectant concentrations up to 3% before a decline in viability was observed.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2011.03.001</identifier><identifier>PMID: 21470802</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>acrosome reaction ; Acrosome Reaction - physiology ; Animals ; Cell Survival - drug effects ; cell viability ; Cryopreservation ; Cryopreservation - methods ; Cryopreservation - veterinary ; Cryoprotectant ; cryoprotectants ; Cryoprotective Agents ; ethylene glycol ; Flow Cytometry - veterinary ; freezers ; freezing ; glycerol ; Horses ; Male ; Male heterogeneity ; Osmolar Concentration ; osmotic stress ; Osmotic tolerance ; osmotolerance ; Semen ; Semen Preservation - methods ; Semen Preservation - veterinary ; Sperm Motility - physiology ; spermatozoa ; Stallion semen ; stallions ; thawing</subject><ispartof>Animal reproduction science, 2011-05, Vol.125 (1-4), p.112-118</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c480t-88199c3882ca21f333fe6257fcc6222e84c9565a66d2bf34e2cabd0734c029863</citedby><cites>FETCH-LOGICAL-c480t-88199c3882ca21f333fe6257fcc6222e84c9565a66d2bf34e2cabd0734c029863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378432011000716$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21470802$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hoffmann, N</creatorcontrib><creatorcontrib>Oldenhof, H</creatorcontrib><creatorcontrib>Morandini, C</creatorcontrib><creatorcontrib>Rohn, K</creatorcontrib><creatorcontrib>Sieme, H</creatorcontrib><title>Optimal concentrations of cryoprotective agents for semen from stallions that are classified ‘good’ or ‘poor’ for freezing</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>Cryopreserved stallion sperm displays a high degree of male-to-male variability with respect to cell viability after thawing. Animals that have semen with low viability after cryopreservation are classified as ‘poor' freezers, and when post-thaw viability is high they are designated as ‘good' freezers. Cryoprotective agents that are used for cryopreserving stallion sperm include glycerol, ethylene glycol, methyl formamide, and dimethylformamide, and are typically used in concentrations ranging from 1% to 4%. The aim of this study was to evaluate the osmotic stresses that stallion sperm is exposed to during cryopreservation, and to determine if sperm from ‘good' and ‘poor' freezers show differences in osmotic tolerance limits and in the suitability of cryoprotective agents. Concentrations of 2-3% of the above mentioned cryoprotectants with freezing extender osmolalities ranging from 580 to 895mOsmkg⁻¹ showed the highest motility rates after freeze-thaw, both for ‘good' and ‘poor' freezers, for all cryoprotectants tested with slightly higher values for glycerol. Freeze-thawed semen from ‘poor' freezers was found to have a lower percentage of progressively motile sperm compared to that of ‘good' freezers. Assessment of plasma and acrosomal membrane integrity after return to isosmotic conditions revealed that cryopreserved sperm from ‘poor' freezers showed lower osmotic tolerance limits as compared to sperm from ‘good' freezers. Semen from ‘poor' freezers that was frozen using freezing extenders supplemented with more then 2% cryoprotectant showed decreased viability and increased acrosome reaction upon return to isoosmotic conditions, whereas ‘good' freezers could withstand cryoprotectant concentrations up to 3% before a decline in viability was observed.</description><subject>acrosome reaction</subject><subject>Acrosome Reaction - physiology</subject><subject>Animals</subject><subject>Cell Survival - drug effects</subject><subject>cell viability</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - veterinary</subject><subject>Cryoprotectant</subject><subject>cryoprotectants</subject><subject>Cryoprotective Agents</subject><subject>ethylene glycol</subject><subject>Flow Cytometry - veterinary</subject><subject>freezers</subject><subject>freezing</subject><subject>glycerol</subject><subject>Horses</subject><subject>Male</subject><subject>Male heterogeneity</subject><subject>Osmolar Concentration</subject><subject>osmotic stress</subject><subject>Osmotic tolerance</subject><subject>osmotolerance</subject><subject>Semen</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - veterinary</subject><subject>Sperm Motility - physiology</subject><subject>spermatozoa</subject><subject>Stallion semen</subject><subject>stallions</subject><subject>thawing</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhS0EokPhFcCsukrqn8RxlmhEC1KlLkrXlse5HjxK4sH2VGpXFU8Br9cn6R2mIFaIlXXl71wfn0PIe85qzrg63dR2Dgm2KWYXasE4r5msGePPyILrTlZCSPGcLJjsdNVIwY7Iq5w3jLFOqf4lORK86ZhmYkG-X25LmOxIXZwdzCXZEuKcafTUpduITxRwJdwAtWu8ztTHRDNMMFOf4kRzseP4S1G-2kJtAupGm3PwAQb6cP9jHePwcP-TogynbYxpP-23-ARwF-b1a_LC2zHDm6fzmFyfffyy_FRdXJ5_Xn64qFyjWam05n3vpNbCWcG9lNKDEm3nnVNCCNCN61vVWqUGsfKyAeRWA-tk45jotZLH5OSwFz_1bQe5mClkB-NoZ4i7bHQnWtE1QiPZH0iHCecE3mwThpRuDWdm34DZmL8aMPsGDJMGG0Dt26dXdqsJhj_K35Ej8O4AeBuNXaeQzfUVbmiwHtnwVv6T4ApdIrE8EIB53QRIBm0AFjigKVfMEMN_WH0EMGC1zA</recordid><startdate>20110501</startdate><enddate>20110501</enddate><creator>Hoffmann, N</creator><creator>Oldenhof, H</creator><creator>Morandini, C</creator><creator>Rohn, K</creator><creator>Sieme, H</creator><general>Elsevier B.V</general><general>[Amsterdam]: Elsevier Science</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110501</creationdate><title>Optimal concentrations of cryoprotective agents for semen from stallions that are classified ‘good’ or ‘poor’ for freezing</title><author>Hoffmann, N ; Oldenhof, H ; Morandini, C ; Rohn, K ; Sieme, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c480t-88199c3882ca21f333fe6257fcc6222e84c9565a66d2bf34e2cabd0734c029863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>acrosome reaction</topic><topic>Acrosome Reaction - physiology</topic><topic>Animals</topic><topic>Cell Survival - drug effects</topic><topic>cell viability</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation - veterinary</topic><topic>Cryoprotectant</topic><topic>cryoprotectants</topic><topic>Cryoprotective Agents</topic><topic>ethylene glycol</topic><topic>Flow Cytometry - veterinary</topic><topic>freezers</topic><topic>freezing</topic><topic>glycerol</topic><topic>Horses</topic><topic>Male</topic><topic>Male heterogeneity</topic><topic>Osmolar Concentration</topic><topic>osmotic stress</topic><topic>Osmotic tolerance</topic><topic>osmotolerance</topic><topic>Semen</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - veterinary</topic><topic>Sperm Motility - physiology</topic><topic>spermatozoa</topic><topic>Stallion semen</topic><topic>stallions</topic><topic>thawing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hoffmann, N</creatorcontrib><creatorcontrib>Oldenhof, H</creatorcontrib><creatorcontrib>Morandini, C</creatorcontrib><creatorcontrib>Rohn, K</creatorcontrib><creatorcontrib>Sieme, H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hoffmann, N</au><au>Oldenhof, H</au><au>Morandini, C</au><au>Rohn, K</au><au>Sieme, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimal concentrations of cryoprotective agents for semen from stallions that are classified ‘good’ or ‘poor’ for freezing</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2011-05-01</date><risdate>2011</risdate><volume>125</volume><issue>1-4</issue><spage>112</spage><epage>118</epage><pages>112-118</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>Cryopreserved stallion sperm displays a high degree of male-to-male variability with respect to cell viability after thawing. Animals that have semen with low viability after cryopreservation are classified as ‘poor' freezers, and when post-thaw viability is high they are designated as ‘good' freezers. Cryoprotective agents that are used for cryopreserving stallion sperm include glycerol, ethylene glycol, methyl formamide, and dimethylformamide, and are typically used in concentrations ranging from 1% to 4%. The aim of this study was to evaluate the osmotic stresses that stallion sperm is exposed to during cryopreservation, and to determine if sperm from ‘good' and ‘poor' freezers show differences in osmotic tolerance limits and in the suitability of cryoprotective agents. Concentrations of 2-3% of the above mentioned cryoprotectants with freezing extender osmolalities ranging from 580 to 895mOsmkg⁻¹ showed the highest motility rates after freeze-thaw, both for ‘good' and ‘poor' freezers, for all cryoprotectants tested with slightly higher values for glycerol. Freeze-thawed semen from ‘poor' freezers was found to have a lower percentage of progressively motile sperm compared to that of ‘good' freezers. Assessment of plasma and acrosomal membrane integrity after return to isosmotic conditions revealed that cryopreserved sperm from ‘poor' freezers showed lower osmotic tolerance limits as compared to sperm from ‘good' freezers. Semen from ‘poor' freezers that was frozen using freezing extenders supplemented with more then 2% cryoprotectant showed decreased viability and increased acrosome reaction upon return to isoosmotic conditions, whereas ‘good' freezers could withstand cryoprotectant concentrations up to 3% before a decline in viability was observed.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21470802</pmid><doi>10.1016/j.anireprosci.2011.03.001</doi><tpages>7</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0378-4320 |
ispartof | Animal reproduction science, 2011-05, Vol.125 (1-4), p.112-118 |
issn | 0378-4320 1873-2232 |
language | eng |
recordid | cdi_proquest_miscellaneous_872527428 |
source | MEDLINE; Elsevier ScienceDirect Journals |
subjects | acrosome reaction Acrosome Reaction - physiology Animals Cell Survival - drug effects cell viability Cryopreservation Cryopreservation - methods Cryopreservation - veterinary Cryoprotectant cryoprotectants Cryoprotective Agents ethylene glycol Flow Cytometry - veterinary freezers freezing glycerol Horses Male Male heterogeneity Osmolar Concentration osmotic stress Osmotic tolerance osmotolerance Semen Semen Preservation - methods Semen Preservation - veterinary Sperm Motility - physiology spermatozoa Stallion semen stallions thawing |
title | Optimal concentrations of cryoprotective agents for semen from stallions that are classified ‘good’ or ‘poor’ for freezing |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T06%3A23%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Optimal%20concentrations%20of%20cryoprotective%20agents%20for%20semen%20from%20stallions%20that%20are%20classified%20%E2%80%98good%E2%80%99%20or%20%E2%80%98poor%E2%80%99%20for%20freezing&rft.jtitle=Animal%20reproduction%20science&rft.au=Hoffmann,%20N&rft.date=2011-05-01&rft.volume=125&rft.issue=1-4&rft.spage=112&rft.epage=118&rft.pages=112-118&rft.issn=0378-4320&rft.eissn=1873-2232&rft_id=info:doi/10.1016/j.anireprosci.2011.03.001&rft_dat=%3Cproquest_cross%3E872527428%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=872527428&rft_id=info:pmid/21470802&rft_els_id=S0378432011000716&rfr_iscdi=true |