Amperometric Thick-Film Strip Electrodes for Monitoring Organophosphate Nerve Agents Based on Immobilized Organophosphorus Hydrolase
An amperometric biosensor based on the immobilization of organophosphorus hydrolase (OPH) onto screen-printed carbon electrodes is shown useful for the rapid, sensitive, and low-cost detection of organophosphate (OP) nerve agents. The sensor relies upon the sensitive and rapid anodic detection of th...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1999-06, Vol.71 (11), p.2246-2249 |
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| creator | Mulchandani, Ashok Mulchandani, Priti Chen, Wilfred Wang, Joseph Chen, Liang |
| description | An amperometric biosensor based on the immobilization of organophosphorus hydrolase (OPH) onto screen-printed carbon electrodes is shown useful for the rapid, sensitive, and low-cost detection of organophosphate (OP) nerve agents. The sensor relies upon the sensitive and rapid anodic detection of the enzymatically generated p-nitrophenol product at the OPH/Nafion layer immobilized onto the thick-film electrode in the presence of the OP substrate. The amperometric signals are linearly proportional to the concentration of the hydrolyzed paraoxon and methyl parathion substrates up to 40 and 5 μM, showing detection limits of 9 × 10-8 and 7 × 10-8 M, respectively. Such detection limits are substantially lower compared to the (2−5) × 10-6 M values reported for OPH-based potentiometric and fiber-optic devices. The high sensitivity is coupled to a faster and simplified operation, and the sensor manifests a selective response compared to analogous enzyme inhibition biosensors. The applicability to river water sampling is illustrated. The attractive performance and greatly simplified operation holds great promise for on-site monitoring of OP pesticides. |
| doi_str_mv | 10.1021/ac9813179 |
| format | Article |
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Chem</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>71</volume><issue>11</issue><spage>2246</spage><epage>2249</epage><pages>2246-2249</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>An amperometric biosensor based on the immobilization of organophosphorus hydrolase (OPH) onto screen-printed carbon electrodes is shown useful for the rapid, sensitive, and low-cost detection of organophosphate (OP) nerve agents. The sensor relies upon the sensitive and rapid anodic detection of the enzymatically generated p-nitrophenol product at the OPH/Nafion layer immobilized onto the thick-film electrode in the presence of the OP substrate. The amperometric signals are linearly proportional to the concentration of the hydrolyzed paraoxon and methyl parathion substrates up to 40 and 5 μM, showing detection limits of 9 × 10-8 and 7 × 10-8 M, respectively. Such detection limits are substantially lower compared to the (2−5) × 10-6 M values reported for OPH-based potentiometric and fiber-optic devices. The high sensitivity is coupled to a faster and simplified operation, and the sensor manifests a selective response compared to analogous enzyme inhibition biosensors. The applicability to river water sampling is illustrated. The attractive performance and greatly simplified operation holds great promise for on-site monitoring of OP pesticides.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>21662762</pmid><doi>10.1021/ac9813179</doi><tpages>4</tpages></addata></record> |
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| subjects | Biological and medical sciences Biosensors Biotechnology Chemistry Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Pesticides Sensors Various methods and equipments |
| title | Amperometric Thick-Film Strip Electrodes for Monitoring Organophosphate Nerve Agents Based on Immobilized Organophosphorus Hydrolase |
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