Secreted autotransporter toxin (Sat) triggers autophagy in epithelial cells that relies on cell detachment

Summary The secreted autotransporter toxin, Sat, which belongs to the subfamily of serine protease autotransporters of Enterobacteriaceae, acts as a virulence factor in extraintestinal and intestinal pathogenic strains of Escherichia coli. We observed that HeLa cells exposed to the cell‐free culture supernatant of recombinant strain AAEC185pSat‐IH11128 producing the Sat toxin (CFCSSat), displayed dramatic disorganization of the F‐actin cytoskeleton before loosening cell‐to‐cell junctions and detachment. Examination of the effect of Sat on GFP‐microtubule‐associated protein light chain 3 (LC3) HeLa cells revealed that CFCSSat‐induced autophagy follows CFCSSat‐induced F‐actin cytoskeleton rearrangement. The induced autophagy shows an acceleration of the autophagy flux soon after Sat treatment, followed later by a blockade of the flux leading to the accumulation of large GFP‐LC3‐positive vacuoles in the cell cytoplasm. CFCSSat did not induce cell detachment in autophagy‐deficient mouse embryonic fibroblasts in contrast with wild‐type mouse embryonic fibroblasts. The CFCSSat‐induced large GFP‐LC3 dots do not display the characteristics of autophagolysosomes including expression of cathepsin D and Lamp‐1 and 2 proteins, and Lysotracker Red‐ and DQ‐BSA‐positive labelling. We provide evidences that CFCSSat‐induced autophagy is not a cell response intended to get rid of the intracellular toxin. By a pharmacological blockers approach, we found that the blockade of Erk1/2 and p38 MAPKs, but not JNK, inhibited the CFCSSat‐induced autophagy and cell detachment whereas phosphatidylinositol‐3 kinase blockers inhibiting canonical autophagy were inactive. When attached CFCSSat‐treated cells start to detach they showed caspase‐independent cell death and rearrangements of the focal adhesion‐associated vinculin and paxillin. Collectively, our results support that Sat triggers autophagy in epithelial cells that relies on its cell‐detachment effect.