Staphylococcus aureus Biofilms: Nemesis of Endoscopic Sinus Surgery
Chronic rhinosinusitis (CRS) patients with biofilms have persistent postoperative symptoms, ongoing mucosal inflammation, and recurrent infections. Recent evidence suggests that biofilms of differing species confer varying disease profiles in CRS patients. We aimed to prospectively investigate the e...
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| Veröffentlicht in: | The Laryngoscope 2011-07, Vol.121 (7), p.1578-1583 |
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| creator | SINGHAL, Deepti FOREMAN, Andrew BARDY, Josh-Jervis WORMALD, Peter-John |
| description | Chronic rhinosinusitis (CRS) patients with biofilms have persistent postoperative symptoms, ongoing mucosal inflammation, and recurrent infections. Recent evidence suggests that biofilms of differing species confer varying disease profiles in CRS patients. We aimed to prospectively investigate the effects of Staphylococcus aureus, Pseudomonas aeruginosa, Haemophilus influenzae, and fungal biofilms on outcomes following endoscopic sinus surgery (ESS).
Prospective blinded study.
In this prospective blinded study, 39 patients undergoing ESS for CRS assessed their symptoms preoperatively using internationally accepted standardized symptom scoring systems and quality-of-life measures (10-point visual analog scale, Sino-Nasal Outcome Test-20, global severity of CRS). Their sinonasal mucosa was graded (Lund-Kennedy scale) and extent of radiologic disease on computed tomography scans scored (Lund-McKay scale). Random sinonasal tissue samples were assessed for different bacterial species forming biofilms by using fluorescent in-situ hybridization and confocal laser microscopy. For 12 months after surgery, CRS symptoms, quality of life, and objective evidence of persisting disease were assessed by using the preoperative tools.
Different bacterial species combinations were found in 30 of 39 patients; 60% of these 30 biofilms were polymicrobial biofilms and 70% had S aureus biofilms. Preoperative nasendoscopy and radiologic disease severity were significantly worse in patients with multiple biofilms (P = .02 and P = .01, respectively), and they had worse postsurgery mucosal outcomes on endoscopy (P = .01) requiring significantly more postoperative visits (P = .04). Those with S aureus biofilms progressed poorly with their symptom scores and quality-of-life outcomes, with significant differences in nasendoscopy scores (P = .007).
S. aureus biofilms play a dominant role in negatively affecting outcomes of ESS with persisting postoperative symptoms, ongoing mucosal inflammation, and infections. |
| doi_str_mv | 10.1002/lary.21805 |
| format | Article |
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Prospective blinded study.
In this prospective blinded study, 39 patients undergoing ESS for CRS assessed their symptoms preoperatively using internationally accepted standardized symptom scoring systems and quality-of-life measures (10-point visual analog scale, Sino-Nasal Outcome Test-20, global severity of CRS). Their sinonasal mucosa was graded (Lund-Kennedy scale) and extent of radiologic disease on computed tomography scans scored (Lund-McKay scale). Random sinonasal tissue samples were assessed for different bacterial species forming biofilms by using fluorescent in-situ hybridization and confocal laser microscopy. For 12 months after surgery, CRS symptoms, quality of life, and objective evidence of persisting disease were assessed by using the preoperative tools.
Different bacterial species combinations were found in 30 of 39 patients; 60% of these 30 biofilms were polymicrobial biofilms and 70% had S aureus biofilms. Preoperative nasendoscopy and radiologic disease severity were significantly worse in patients with multiple biofilms (P = .02 and P = .01, respectively), and they had worse postsurgery mucosal outcomes on endoscopy (P = .01) requiring significantly more postoperative visits (P = .04). Those with S aureus biofilms progressed poorly with their symptom scores and quality-of-life outcomes, with significant differences in nasendoscopy scores (P = .007).
S. aureus biofilms play a dominant role in negatively affecting outcomes of ESS with persisting postoperative symptoms, ongoing mucosal inflammation, and infections.</description><identifier>ISSN: 0023-852X</identifier><identifier>EISSN: 1531-4995</identifier><identifier>DOI: 10.1002/lary.21805</identifier><identifier>PMID: 21647904</identifier><identifier>CODEN: LARYA8</identifier><language>eng</language><publisher>Hoboken, NJ: Wiley-Blackwell</publisher><subject>Adult ; Analysis of Variance ; Biofilms - growth & development ; Biological and medical sciences ; Chronic Disease ; Endoscopy - adverse effects ; Endoscopy - methods ; Female ; Follow-Up Studies ; Humans ; Male ; Medical sciences ; Middle Aged ; Non tumoral diseases ; Otorhinolaryngologic Surgical Procedures - adverse effects ; Otorhinolaryngologic Surgical Procedures - methods ; Otorhinolaryngology (head neck, general aspects and miscellaneous) ; Otorhinolaryngology. Stomatology ; Paranasal Sinuses - microbiology ; Paranasal Sinuses - surgery ; Postoperative Complications - epidemiology ; Postoperative Complications - microbiology ; Predictive Value of Tests ; Preoperative Care - methods ; Prospective Studies ; Rhinitis - microbiology ; Rhinitis - surgery ; Risk Assessment ; Single-Blind Method ; Sinusitis - microbiology ; Sinusitis - surgery ; Staphylococcal Infections - diagnosis ; Staphylococcal Infections - surgery ; Staphylococcus aureus - physiology ; Statistics, Nonparametric ; Time Factors ; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><ispartof>The Laryngoscope, 2011-07, Vol.121 (7), p.1578-1583</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 The American Laryngological, Rhinological, and Otological Society, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c275t-e9033d282dd868a007fad749f921edad8acce34686d0022fa024fbc8c13817cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24336258$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21647904$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SINGHAL, Deepti</creatorcontrib><creatorcontrib>FOREMAN, Andrew</creatorcontrib><creatorcontrib>BARDY, Josh-Jervis</creatorcontrib><creatorcontrib>WORMALD, Peter-John</creatorcontrib><title>Staphylococcus aureus Biofilms: Nemesis of Endoscopic Sinus Surgery</title><title>The Laryngoscope</title><addtitle>Laryngoscope</addtitle><description>Chronic rhinosinusitis (CRS) patients with biofilms have persistent postoperative symptoms, ongoing mucosal inflammation, and recurrent infections. Recent evidence suggests that biofilms of differing species confer varying disease profiles in CRS patients. We aimed to prospectively investigate the effects of Staphylococcus aureus, Pseudomonas aeruginosa, Haemophilus influenzae, and fungal biofilms on outcomes following endoscopic sinus surgery (ESS).
Prospective blinded study.
In this prospective blinded study, 39 patients undergoing ESS for CRS assessed their symptoms preoperatively using internationally accepted standardized symptom scoring systems and quality-of-life measures (10-point visual analog scale, Sino-Nasal Outcome Test-20, global severity of CRS). Their sinonasal mucosa was graded (Lund-Kennedy scale) and extent of radiologic disease on computed tomography scans scored (Lund-McKay scale). Random sinonasal tissue samples were assessed for different bacterial species forming biofilms by using fluorescent in-situ hybridization and confocal laser microscopy. For 12 months after surgery, CRS symptoms, quality of life, and objective evidence of persisting disease were assessed by using the preoperative tools.
Different bacterial species combinations were found in 30 of 39 patients; 60% of these 30 biofilms were polymicrobial biofilms and 70% had S aureus biofilms. Preoperative nasendoscopy and radiologic disease severity were significantly worse in patients with multiple biofilms (P = .02 and P = .01, respectively), and they had worse postsurgery mucosal outcomes on endoscopy (P = .01) requiring significantly more postoperative visits (P = .04). Those with S aureus biofilms progressed poorly with their symptom scores and quality-of-life outcomes, with significant differences in nasendoscopy scores (P = .007).
S. aureus biofilms play a dominant role in negatively affecting outcomes of ESS with persisting postoperative symptoms, ongoing mucosal inflammation, and infections.</description><subject>Adult</subject><subject>Analysis of Variance</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>Chronic Disease</subject><subject>Endoscopy - adverse effects</subject><subject>Endoscopy - methods</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Non tumoral diseases</subject><subject>Otorhinolaryngologic Surgical Procedures - adverse effects</subject><subject>Otorhinolaryngologic Surgical Procedures - methods</subject><subject>Otorhinolaryngology (head neck, general aspects and miscellaneous)</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Paranasal Sinuses - microbiology</subject><subject>Paranasal Sinuses - surgery</subject><subject>Postoperative Complications - epidemiology</subject><subject>Postoperative Complications - microbiology</subject><subject>Predictive Value of Tests</subject><subject>Preoperative Care - methods</subject><subject>Prospective Studies</subject><subject>Rhinitis - microbiology</subject><subject>Rhinitis - surgery</subject><subject>Risk Assessment</subject><subject>Single-Blind Method</subject><subject>Sinusitis - microbiology</subject><subject>Sinusitis - surgery</subject><subject>Staphylococcal Infections - diagnosis</subject><subject>Staphylococcal Infections - surgery</subject><subject>Staphylococcus aureus - physiology</subject><subject>Statistics, Nonparametric</subject><subject>Time Factors</subject><subject>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</subject><issn>0023-852X</issn><issn>1531-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0E1LAzEQgOEgiq3Viz9A9iKCsDVfu8l601I_oOihCt6WdJJoZLdZk-6h_97UVj3NYR6G4UXolOAxwZheNSqsx5RIXOyhISkYyXlVFftomJYslwV9G6CjGD8xJoIV-BANKCm5qDAfosl8pbqPdePBA_QxU30wadw6b13TxuvsybQmuph5m02X2kfwnYNs7pZJzfvwbsL6GB1Y1URzspsj9Ho3fZk85LPn-8fJzSwHKopVbirMmKaSai1LqTAWVmnBK1tRYrTSUgEYxktZ6vQ4tQpTbhcggTBJBGg2Qhfbu13wX72Jq7p1EUzTqKXxfayloJwJxnCSl1sJwccYjK274NqUqSa43jSrN83qn2YJn-3O9ovW6D_6GymB8x1QEVRjg1qCi_-OM1bSQrJvYDd0sg</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>SINGHAL, Deepti</creator><creator>FOREMAN, Andrew</creator><creator>BARDY, Josh-Jervis</creator><creator>WORMALD, Peter-John</creator><general>Wiley-Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110701</creationdate><title>Staphylococcus aureus Biofilms: Nemesis of Endoscopic Sinus Surgery</title><author>SINGHAL, Deepti ; FOREMAN, Andrew ; BARDY, Josh-Jervis ; WORMALD, Peter-John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c275t-e9033d282dd868a007fad749f921edad8acce34686d0022fa024fbc8c13817cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adult</topic><topic>Analysis of Variance</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>Chronic Disease</topic><topic>Endoscopy - adverse effects</topic><topic>Endoscopy - methods</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Non tumoral diseases</topic><topic>Otorhinolaryngologic Surgical Procedures - adverse effects</topic><topic>Otorhinolaryngologic Surgical Procedures - methods</topic><topic>Otorhinolaryngology (head neck, general aspects and miscellaneous)</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Paranasal Sinuses - microbiology</topic><topic>Paranasal Sinuses - surgery</topic><topic>Postoperative Complications - epidemiology</topic><topic>Postoperative Complications - microbiology</topic><topic>Predictive Value of Tests</topic><topic>Preoperative Care - methods</topic><topic>Prospective Studies</topic><topic>Rhinitis - microbiology</topic><topic>Rhinitis - surgery</topic><topic>Risk Assessment</topic><topic>Single-Blind Method</topic><topic>Sinusitis - microbiology</topic><topic>Sinusitis - surgery</topic><topic>Staphylococcal Infections - diagnosis</topic><topic>Staphylococcal Infections - surgery</topic><topic>Staphylococcus aureus - physiology</topic><topic>Statistics, Nonparametric</topic><topic>Time Factors</topic><topic>Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SINGHAL, Deepti</creatorcontrib><creatorcontrib>FOREMAN, Andrew</creatorcontrib><creatorcontrib>BARDY, Josh-Jervis</creatorcontrib><creatorcontrib>WORMALD, Peter-John</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Laryngoscope</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SINGHAL, Deepti</au><au>FOREMAN, Andrew</au><au>BARDY, Josh-Jervis</au><au>WORMALD, Peter-John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Staphylococcus aureus Biofilms: Nemesis of Endoscopic Sinus Surgery</atitle><jtitle>The Laryngoscope</jtitle><addtitle>Laryngoscope</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>121</volume><issue>7</issue><spage>1578</spage><epage>1583</epage><pages>1578-1583</pages><issn>0023-852X</issn><eissn>1531-4995</eissn><coden>LARYA8</coden><abstract>Chronic rhinosinusitis (CRS) patients with biofilms have persistent postoperative symptoms, ongoing mucosal inflammation, and recurrent infections. Recent evidence suggests that biofilms of differing species confer varying disease profiles in CRS patients. We aimed to prospectively investigate the effects of Staphylococcus aureus, Pseudomonas aeruginosa, Haemophilus influenzae, and fungal biofilms on outcomes following endoscopic sinus surgery (ESS).
Prospective blinded study.
In this prospective blinded study, 39 patients undergoing ESS for CRS assessed their symptoms preoperatively using internationally accepted standardized symptom scoring systems and quality-of-life measures (10-point visual analog scale, Sino-Nasal Outcome Test-20, global severity of CRS). Their sinonasal mucosa was graded (Lund-Kennedy scale) and extent of radiologic disease on computed tomography scans scored (Lund-McKay scale). Random sinonasal tissue samples were assessed for different bacterial species forming biofilms by using fluorescent in-situ hybridization and confocal laser microscopy. For 12 months after surgery, CRS symptoms, quality of life, and objective evidence of persisting disease were assessed by using the preoperative tools.
Different bacterial species combinations were found in 30 of 39 patients; 60% of these 30 biofilms were polymicrobial biofilms and 70% had S aureus biofilms. Preoperative nasendoscopy and radiologic disease severity were significantly worse in patients with multiple biofilms (P = .02 and P = .01, respectively), and they had worse postsurgery mucosal outcomes on endoscopy (P = .01) requiring significantly more postoperative visits (P = .04). Those with S aureus biofilms progressed poorly with their symptom scores and quality-of-life outcomes, with significant differences in nasendoscopy scores (P = .007).
S. aureus biofilms play a dominant role in negatively affecting outcomes of ESS with persisting postoperative symptoms, ongoing mucosal inflammation, and infections.</abstract><cop>Hoboken, NJ</cop><pub>Wiley-Blackwell</pub><pmid>21647904</pmid><doi>10.1002/lary.21805</doi><tpages>6</tpages></addata></record> |
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| subjects | Adult Analysis of Variance Biofilms - growth & development Biological and medical sciences Chronic Disease Endoscopy - adverse effects Endoscopy - methods Female Follow-Up Studies Humans Male Medical sciences Middle Aged Non tumoral diseases Otorhinolaryngologic Surgical Procedures - adverse effects Otorhinolaryngologic Surgical Procedures - methods Otorhinolaryngology (head neck, general aspects and miscellaneous) Otorhinolaryngology. Stomatology Paranasal Sinuses - microbiology Paranasal Sinuses - surgery Postoperative Complications - epidemiology Postoperative Complications - microbiology Predictive Value of Tests Preoperative Care - methods Prospective Studies Rhinitis - microbiology Rhinitis - surgery Risk Assessment Single-Blind Method Sinusitis - microbiology Sinusitis - surgery Staphylococcal Infections - diagnosis Staphylococcal Infections - surgery Staphylococcus aureus - physiology Statistics, Nonparametric Time Factors Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology |
| title | Staphylococcus aureus Biofilms: Nemesis of Endoscopic Sinus Surgery |
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