Monoclonal antibody-based triple antibody sandwich-enzyme-linked immunosorbent assay and immunocapture reverse transcription-polymerase chain reaction for Odontoglossum ringspot virus detection

Odontoglossum ringspot virus (ORSV) infects numerous commercially important orchids and causes significant losses worldwide. The coat protein ( CP) gene of ORSV was cloned and expressed in Escherichia coli by using the pET-32a expression vector, and the expression of recombinant protein was confirme...

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Veröffentlicht in:	Journal of virological methods 2011, Vol.171 (1), p.40-45
Hauptverfasser:	Wu, Jianxiang, Meng, Chunmei, Shang, Haili, Rong, Song, Zhang, Chao, Hong, Jian, Zhou, Xueping
Format:	Artikel
Sprache:	eng
Schlagworte:	agarose Animals Antibodies Antibodies, Monoclonal - isolation & purification Antibodies, Viral - isolation & purification antigens Antigens, Viral - genetics Antigens, Viral - immunology Antigens, Viral - isolation & purification Biological and medical sciences Capsid Proteins - genetics China Coat protein coat proteins CP gene double prime CP gene Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Escherichia coli Escherichia coli - genetics Expression vectors Fundamental and applied biological sciences. Psychology Gene Expression genes Immunocapture RT-PCR Immunosorbents Leaves Mice Microbiology Monoclonal antibodies Monoclonal antibody Odontoglossum ringspot virus Orchid Orchidaceae Orchidaceae - virology Phytopathology. Animal pests. Plant and forest protection Plant Diseases - virology Plant viruses and viroids polyclonal antibodies rapid methods recombinant proteins Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - isolation & purification reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - methods SAP protein Sensitivity and Specificity Techniques used in virology Tobamovirus - isolation & purification Virology Virology - methods Western blotting
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