Comparison of the Effects of NADH- and NADPH-Perturbation Stresses on the Growth of Escherichia coli
To better understand how the reducing power of either NADH or NADPH affects cell growth, Escherichia coli strains expressing either NADH-dependent or NADPH-dependent azoreductase (EC 1.6.5.2), which mediates the reduction of an azo dye, were cultured in glucose minimal medium in the presence of 200...
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| Veröffentlicht in: | Current microbiology 2009-02, Vol.58 (2), p.159-163 |
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| creator | Kim, Susie Moon, Doo-Bum Lee, Chung-Hwan Nam, Soo-Wan Kim, Pil |
| description | To better understand how the reducing power of either NADH or NADPH affects cell growth,
Escherichia coli
strains expressing either NADH-dependent or NADPH-dependent azoreductase (EC 1.6.5.2), which mediates the reduction of an azo dye, were cultured in glucose minimal medium in the presence of 200 μM methyl red. Growth rates in NADH-perturbed, NADPH-perturbed, and control cells were 0.05, 0.12, and 0.13 h
−1
, respectively. In addition, glucose consumption in NADH-perturbed cells was 10.8 g glucose/g cell, while that of control and NADPH-perturbed cells was very similar (3.6 vs 3.8 g glucose/g cell) during the perturbation phase. Therefore, NADH perturbation had a larger effect than NADPH on cellular growth. |
| doi_str_mv | 10.1007/s00284-008-9294-6 |
| format | Article |
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Escherichia coli
strains expressing either NADH-dependent or NADPH-dependent azoreductase (EC 1.6.5.2), which mediates the reduction of an azo dye, were cultured in glucose minimal medium in the presence of 200 μM methyl red. Growth rates in NADH-perturbed, NADPH-perturbed, and control cells were 0.05, 0.12, and 0.13 h
−1
, respectively. In addition, glucose consumption in NADH-perturbed cells was 10.8 g glucose/g cell, while that of control and NADPH-perturbed cells was very similar (3.6 vs 3.8 g glucose/g cell) during the perturbation phase. Therefore, NADH perturbation had a larger effect than NADPH on cellular growth.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-008-9294-6</identifier><identifier>PMID: 18953603</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Azo dyes ; Bacteriology ; Biomedical and Life Sciences ; Biotechnology ; E coli ; Escherichia coli ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Escherichia coli - growth & development ; Escherichia coli - metabolism ; Escherichia coli Proteins - genetics ; Escherichia coli Proteins - metabolism ; Gene Expression ; Glucose - metabolism ; Life Sciences ; Microbiology ; NAD - metabolism ; NADH, NADPH Oxidoreductases - genetics ; NADH, NADPH Oxidoreductases - metabolism ; NADP - metabolism</subject><ispartof>Current microbiology, 2009-02, Vol.58 (2), p.159-163</ispartof><rights>Springer Science+Business Media, LLC 2008</rights><rights>Springer Science+Business Media, LLC 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-ac79d1167b9b6d0758d79a48ab480257b560255bcd1839ba07916ed738b3330a3</citedby><cites>FETCH-LOGICAL-c401t-ac79d1167b9b6d0758d79a48ab480257b560255bcd1839ba07916ed738b3330a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00284-008-9294-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00284-008-9294-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18953603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Susie</creatorcontrib><creatorcontrib>Moon, Doo-Bum</creatorcontrib><creatorcontrib>Lee, Chung-Hwan</creatorcontrib><creatorcontrib>Nam, Soo-Wan</creatorcontrib><creatorcontrib>Kim, Pil</creatorcontrib><title>Comparison of the Effects of NADH- and NADPH-Perturbation Stresses on the Growth of Escherichia coli</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>To better understand how the reducing power of either NADH or NADPH affects cell growth,
Escherichia coli
strains expressing either NADH-dependent or NADPH-dependent azoreductase (EC 1.6.5.2), which mediates the reduction of an azo dye, were cultured in glucose minimal medium in the presence of 200 μM methyl red. Growth rates in NADH-perturbed, NADPH-perturbed, and control cells were 0.05, 0.12, and 0.13 h
−1
, respectively. In addition, glucose consumption in NADH-perturbed cells was 10.8 g glucose/g cell, while that of control and NADPH-perturbed cells was very similar (3.6 vs 3.8 g glucose/g cell) during the perturbation phase. Therefore, NADH perturbation had a larger effect than NADPH on cellular growth.</description><subject>Azo dyes</subject><subject>Bacteriology</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - growth & development</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>Gene Expression</subject><subject>Glucose - metabolism</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>NAD - metabolism</subject><subject>NADH, NADPH Oxidoreductases - genetics</subject><subject>NADH, NADPH Oxidoreductases - metabolism</subject><subject>NADP - metabolism</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kU9P3DAQxS1UBFvgA_RSRT3AyXRsJ_5zXC0LWwkBUuFs2Y7TzWo33tqJKr49TrMSUqVyGlv-vTeeeQh9IXBNAMT3BEBliQEkVlSVmB-hGSkZxaAU-YRmwEqGJa_IKfqc0gaAUAXkBJ0SqSrGgc1QvQi7vYltCl0RmqJf-2LZNN71abw-zG9WuDBdPZ6eVvjJx36I1vRtxn_20afkM9j91d3F8Kdfj7JlcmsfW7duTeHCtj1Hx43ZJn9xqGfo5Xb5vFjh-8e7H4v5PXYlkB4bJ1RNCBdWWV6DqGQtlCmlsaUEWglb8Vwq62oimbIGhCLc14JJyxgDw87Q1eS7j-H34FOvd21yfrs1nQ9D0lLQvIGKqkxefkhyLgnkJhn89g-4CUPs8hSaQkXH38oMkQlyMaQUfaP3sd2Z-KoJ6DEpPSWlc1J6TErzrPl6MB7sztfvikM0GaATkPJT98vH987_d30Dm_ubSg</recordid><startdate>20090201</startdate><enddate>20090201</enddate><creator>Kim, Susie</creator><creator>Moon, Doo-Bum</creator><creator>Lee, Chung-Hwan</creator><creator>Nam, Soo-Wan</creator><creator>Kim, Pil</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20090201</creationdate><title>Comparison of the Effects of NADH- and NADPH-Perturbation Stresses on the Growth of Escherichia coli</title><author>Kim, Susie ; Moon, Doo-Bum ; Lee, Chung-Hwan ; Nam, Soo-Wan ; Kim, Pil</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-ac79d1167b9b6d0758d79a48ab480257b560255bcd1839ba07916ed738b3330a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Azo dyes</topic><topic>Bacteriology</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - growth & development</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Escherichia coli Proteins - metabolism</topic><topic>Gene Expression</topic><topic>Glucose - metabolism</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>NAD - metabolism</topic><topic>NADH, NADPH Oxidoreductases - genetics</topic><topic>NADH, NADPH Oxidoreductases - metabolism</topic><topic>NADP - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Susie</creatorcontrib><creatorcontrib>Moon, Doo-Bum</creatorcontrib><creatorcontrib>Lee, Chung-Hwan</creatorcontrib><creatorcontrib>Nam, Soo-Wan</creatorcontrib><creatorcontrib>Kim, Pil</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Susie</au><au>Moon, Doo-Bum</au><au>Lee, Chung-Hwan</au><au>Nam, Soo-Wan</au><au>Kim, Pil</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of the Effects of NADH- and NADPH-Perturbation Stresses on the Growth of Escherichia coli</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2009-02-01</date><risdate>2009</risdate><volume>58</volume><issue>2</issue><spage>159</spage><epage>163</epage><pages>159-163</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>To better understand how the reducing power of either NADH or NADPH affects cell growth,
Escherichia coli
strains expressing either NADH-dependent or NADPH-dependent azoreductase (EC 1.6.5.2), which mediates the reduction of an azo dye, were cultured in glucose minimal medium in the presence of 200 μM methyl red. Growth rates in NADH-perturbed, NADPH-perturbed, and control cells were 0.05, 0.12, and 0.13 h
−1
, respectively. In addition, glucose consumption in NADH-perturbed cells was 10.8 g glucose/g cell, while that of control and NADPH-perturbed cells was very similar (3.6 vs 3.8 g glucose/g cell) during the perturbation phase. Therefore, NADH perturbation had a larger effect than NADPH on cellular growth.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>18953603</pmid><doi>10.1007/s00284-008-9294-6</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Azo dyes Bacteriology Biomedical and Life Sciences Biotechnology E coli Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - metabolism Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Gene Expression Glucose - metabolism Life Sciences Microbiology NAD - metabolism NADH, NADPH Oxidoreductases - genetics NADH, NADPH Oxidoreductases - metabolism NADP - metabolism |
| title | Comparison of the Effects of NADH- and NADPH-Perturbation Stresses on the Growth of Escherichia coli |
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