Msi-1 is a Predictor of Survival and a Novel Therapeutic Target in Colon Cancer
Background Musashi1 (Msi-1), a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Recently, aberrant expression of Msi-1 has been detected in several malignancies. However, its role in the progression of colon cancer is largely unknown. Materia...
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| Veröffentlicht in: | Annals of surgical oncology 2011-07, Vol.18 (7), p.2074-2083 |
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| creator | Li, Dawei Peng, Xiao Yan, Dongwang Tang, Huamei Huang, Fei Yang, Yinxue Peng, Zhihai |
| description | Background
Musashi1 (Msi-1), a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Recently, aberrant expression of Msi-1 has been detected in several malignancies. However, its role in the progression of colon cancer is largely unknown.
Materials and Methods
We used Western blotting to examine Msi-1 protein expression in 8 cases of primary colon cancer lesions and paired normal colonic mucosa. Msi-1 expression and clinicopathological significance were determined by immunohistochemical staining in a tissue microarray (TMA) containing 203 cases of primary colon cancer paired with noncancerous tissue and 66 lymph node metastasis (LNM) tissues. RNAi was used to analyze the biological function of Msi-1 in vitro.
Results
LNM tissue exhibited a striking increase in Msi-1 expression when compared with primary colon cancer and adjacent normal mucosa (87.9% vs. 64.5% vs. 16.7%,
P
|
| doi_str_mv | 10.1245/s10434-011-1567-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_872127243</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2374770531</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-9bd48470f89c7b54872e84d99f531fe77d138b3d19eac410089ce8ffdaafc1113</originalsourceid><addsrcrecordid>eNp1kEtLxDAQx4Movj-AFwlePFUzTdI0R1l8gS9wPYdsOtFKt1mTdsFvb2R9gOAlEzK_-Wf4EXIA7ARKIU8TMMFFwQAKkJUq9BrZBplfRFXDer6zqi50WcktspPSK2OgOJObZKsEIUou2Ta5v01tAbRN1NKHiE3rhhBp8PRxjMt2aTtq-yb37sISOzp9wWgXOA6to1Mbn3GgbU8noQv5tL3DuEc2vO0S7n_VXfJ0cT6dXBU395fXk7ObwgleDYWeNaIWivlaOzWTolYl1qLR2ksOHpVqgNcz3oBG6wQwljmsvW-s9Q4A-C45XuUuYngbMQ1m3iaHXWd7DGMyORBKVQqeyaM_5GsYY5-XyxAoJTWXGYIV5GJIKaI3i9jObXw3wMyna7NybbJr8-na6Dxz-BU8zubY_Ex8y81AuQJSbvXPGH9__j_1A6O4h1M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>871775935</pqid></control><display><type>article</type><title>Msi-1 is a Predictor of Survival and a Novel Therapeutic Target in Colon Cancer</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Li, Dawei ; Peng, Xiao ; Yan, Dongwang ; Tang, Huamei ; Huang, Fei ; Yang, Yinxue ; Peng, Zhihai</creator><creatorcontrib>Li, Dawei ; Peng, Xiao ; Yan, Dongwang ; Tang, Huamei ; Huang, Fei ; Yang, Yinxue ; Peng, Zhihai</creatorcontrib><description>Background
Musashi1 (Msi-1), a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Recently, aberrant expression of Msi-1 has been detected in several malignancies. However, its role in the progression of colon cancer is largely unknown.
Materials and Methods
We used Western blotting to examine Msi-1 protein expression in 8 cases of primary colon cancer lesions and paired normal colonic mucosa. Msi-1 expression and clinicopathological significance were determined by immunohistochemical staining in a tissue microarray (TMA) containing 203 cases of primary colon cancer paired with noncancerous tissue and 66 lymph node metastasis (LNM) tissues. RNAi was used to analyze the biological function of Msi-1 in vitro.
Results
LNM tissue exhibited a striking increase in Msi-1 expression when compared with primary colon cancer and adjacent normal mucosa (87.9% vs. 64.5% vs. 16.7%,
P
< .001). Overexpression of Msi-1 was associated with higher clinical stage, T stage, lymph node metastasis, presence of distant metastasis, and Ki-67 positivity. Msi-1 served as an independent prognostic marker whose expression levels correlated with poorer metastasis-free survival (MFS) (HR 5.4;
P
< .001) and poorer overall survival (OS) (HR 3.8;
P
< .001). Msi-1 silencing significantly inhibited proliferation ability and attenuated the migration and invasion activity of colon cancer cells.
Conclusions
Our study provides the basis to explore the use of Msi-1 as a novel prognostic biomarker in colon cancer patients. Aberrant overexpression of Msi-1 during metastasis of colon cancer also suggests that it is a potential therapeutic target.</description><identifier>ISSN: 1068-9265</identifier><identifier>EISSN: 1534-4681</identifier><identifier>DOI: 10.1245/s10434-011-1567-9</identifier><identifier>PMID: 21442350</identifier><language>eng</language><publisher>New York: Springer-Verlag</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Blotting, Western ; Cell Adhesion ; Cell Differentiation ; Cell Movement ; Cell Proliferation ; Colon - metabolism ; Colon - pathology ; Colonic Neoplasms - metabolism ; Colonic Neoplasms - mortality ; Colonic Neoplasms - pathology ; Female ; Follow-Up Studies ; Humans ; Immunoenzyme Techniques ; Lymphatic Metastasis ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Nerve Tissue Proteins - antagonists & inhibitors ; Nerve Tissue Proteins - genetics ; Nerve Tissue Proteins - metabolism ; Oncology ; Prognosis ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Small Interfering - genetics ; RNA-Binding Proteins - antagonists & inhibitors ; RNA-Binding Proteins - genetics ; RNA-Binding Proteins - metabolism ; Surgery ; Surgical Oncology ; Survival Rate ; Tissue Array Analysis ; Translational Research and Biomarkers ; Tumor Cells, Cultured ; Young Adult</subject><ispartof>Annals of surgical oncology, 2011-07, Vol.18 (7), p.2074-2083</ispartof><rights>Society of Surgical Oncology 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-9bd48470f89c7b54872e84d99f531fe77d138b3d19eac410089ce8ffdaafc1113</citedby><cites>FETCH-LOGICAL-c436t-9bd48470f89c7b54872e84d99f531fe77d138b3d19eac410089ce8ffdaafc1113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1245/s10434-011-1567-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1245/s10434-011-1567-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21442350$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Dawei</creatorcontrib><creatorcontrib>Peng, Xiao</creatorcontrib><creatorcontrib>Yan, Dongwang</creatorcontrib><creatorcontrib>Tang, Huamei</creatorcontrib><creatorcontrib>Huang, Fei</creatorcontrib><creatorcontrib>Yang, Yinxue</creatorcontrib><creatorcontrib>Peng, Zhihai</creatorcontrib><title>Msi-1 is a Predictor of Survival and a Novel Therapeutic Target in Colon Cancer</title><title>Annals of surgical oncology</title><addtitle>Ann Surg Oncol</addtitle><addtitle>Ann Surg Oncol</addtitle><description>Background
Musashi1 (Msi-1), a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Recently, aberrant expression of Msi-1 has been detected in several malignancies. However, its role in the progression of colon cancer is largely unknown.
Materials and Methods
We used Western blotting to examine Msi-1 protein expression in 8 cases of primary colon cancer lesions and paired normal colonic mucosa. Msi-1 expression and clinicopathological significance were determined by immunohistochemical staining in a tissue microarray (TMA) containing 203 cases of primary colon cancer paired with noncancerous tissue and 66 lymph node metastasis (LNM) tissues. RNAi was used to analyze the biological function of Msi-1 in vitro.
Results
LNM tissue exhibited a striking increase in Msi-1 expression when compared with primary colon cancer and adjacent normal mucosa (87.9% vs. 64.5% vs. 16.7%,
P
< .001). Overexpression of Msi-1 was associated with higher clinical stage, T stage, lymph node metastasis, presence of distant metastasis, and Ki-67 positivity. Msi-1 served as an independent prognostic marker whose expression levels correlated with poorer metastasis-free survival (MFS) (HR 5.4;
P
< .001) and poorer overall survival (OS) (HR 3.8;
P
< .001). Msi-1 silencing significantly inhibited proliferation ability and attenuated the migration and invasion activity of colon cancer cells.
Conclusions
Our study provides the basis to explore the use of Msi-1 as a novel prognostic biomarker in colon cancer patients. Aberrant overexpression of Msi-1 during metastasis of colon cancer also suggests that it is a potential therapeutic target.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Blotting, Western</subject><subject>Cell Adhesion</subject><subject>Cell Differentiation</subject><subject>Cell Movement</subject><subject>Cell Proliferation</subject><subject>Colon - metabolism</subject><subject>Colon - pathology</subject><subject>Colonic Neoplasms - metabolism</subject><subject>Colonic Neoplasms - mortality</subject><subject>Colonic Neoplasms - pathology</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Nerve Tissue Proteins - antagonists & inhibitors</subject><subject>Nerve Tissue Proteins - genetics</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Oncology</subject><subject>Prognosis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA-Binding Proteins - antagonists & inhibitors</subject><subject>RNA-Binding Proteins - genetics</subject><subject>RNA-Binding Proteins - metabolism</subject><subject>Surgery</subject><subject>Surgical Oncology</subject><subject>Survival Rate</subject><subject>Tissue Array Analysis</subject><subject>Translational Research and Biomarkers</subject><subject>Tumor Cells, Cultured</subject><subject>Young Adult</subject><issn>1068-9265</issn><issn>1534-4681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kEtLxDAQx4Movj-AFwlePFUzTdI0R1l8gS9wPYdsOtFKt1mTdsFvb2R9gOAlEzK_-Wf4EXIA7ARKIU8TMMFFwQAKkJUq9BrZBplfRFXDer6zqi50WcktspPSK2OgOJObZKsEIUou2Ta5v01tAbRN1NKHiE3rhhBp8PRxjMt2aTtq-yb37sISOzp9wWgXOA6to1Mbn3GgbU8noQv5tL3DuEc2vO0S7n_VXfJ0cT6dXBU395fXk7ObwgleDYWeNaIWivlaOzWTolYl1qLR2ksOHpVqgNcz3oBG6wQwljmsvW-s9Q4A-C45XuUuYngbMQ1m3iaHXWd7DGMyORBKVQqeyaM_5GsYY5-XyxAoJTWXGYIV5GJIKaI3i9jObXw3wMyna7NybbJr8-na6Dxz-BU8zubY_Ex8y81AuQJSbvXPGH9__j_1A6O4h1M</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Li, Dawei</creator><creator>Peng, Xiao</creator><creator>Yan, Dongwang</creator><creator>Tang, Huamei</creator><creator>Huang, Fei</creator><creator>Yang, Yinxue</creator><creator>Peng, Zhihai</creator><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20110701</creationdate><title>Msi-1 is a Predictor of Survival and a Novel Therapeutic Target in Colon Cancer</title><author>Li, Dawei ; Peng, Xiao ; Yan, Dongwang ; Tang, Huamei ; Huang, Fei ; Yang, Yinxue ; Peng, Zhihai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-9bd48470f89c7b54872e84d99f531fe77d138b3d19eac410089ce8ffdaafc1113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Blotting, Western</topic><topic>Cell Adhesion</topic><topic>Cell Differentiation</topic><topic>Cell Movement</topic><topic>Cell Proliferation</topic><topic>Colon - metabolism</topic><topic>Colon - pathology</topic><topic>Colonic Neoplasms - metabolism</topic><topic>Colonic Neoplasms - mortality</topic><topic>Colonic Neoplasms - pathology</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Nerve Tissue Proteins - antagonists & inhibitors</topic><topic>Nerve Tissue Proteins - genetics</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Oncology</topic><topic>Prognosis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA-Binding Proteins - antagonists & inhibitors</topic><topic>RNA-Binding Proteins - genetics</topic><topic>RNA-Binding Proteins - metabolism</topic><topic>Surgery</topic><topic>Surgical Oncology</topic><topic>Survival Rate</topic><topic>Tissue Array Analysis</topic><topic>Translational Research and Biomarkers</topic><topic>Tumor Cells, Cultured</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Dawei</creatorcontrib><creatorcontrib>Peng, Xiao</creatorcontrib><creatorcontrib>Yan, Dongwang</creatorcontrib><creatorcontrib>Tang, Huamei</creatorcontrib><creatorcontrib>Huang, Fei</creatorcontrib><creatorcontrib>Yang, Yinxue</creatorcontrib><creatorcontrib>Peng, Zhihai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of surgical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Dawei</au><au>Peng, Xiao</au><au>Yan, Dongwang</au><au>Tang, Huamei</au><au>Huang, Fei</au><au>Yang, Yinxue</au><au>Peng, Zhihai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Msi-1 is a Predictor of Survival and a Novel Therapeutic Target in Colon Cancer</atitle><jtitle>Annals of surgical oncology</jtitle><stitle>Ann Surg Oncol</stitle><addtitle>Ann Surg Oncol</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>18</volume><issue>7</issue><spage>2074</spage><epage>2083</epage><pages>2074-2083</pages><issn>1068-9265</issn><eissn>1534-4681</eissn><abstract>Background
Musashi1 (Msi-1), a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Recently, aberrant expression of Msi-1 has been detected in several malignancies. However, its role in the progression of colon cancer is largely unknown.
Materials and Methods
We used Western blotting to examine Msi-1 protein expression in 8 cases of primary colon cancer lesions and paired normal colonic mucosa. Msi-1 expression and clinicopathological significance were determined by immunohistochemical staining in a tissue microarray (TMA) containing 203 cases of primary colon cancer paired with noncancerous tissue and 66 lymph node metastasis (LNM) tissues. RNAi was used to analyze the biological function of Msi-1 in vitro.
Results
LNM tissue exhibited a striking increase in Msi-1 expression when compared with primary colon cancer and adjacent normal mucosa (87.9% vs. 64.5% vs. 16.7%,
P
< .001). Overexpression of Msi-1 was associated with higher clinical stage, T stage, lymph node metastasis, presence of distant metastasis, and Ki-67 positivity. Msi-1 served as an independent prognostic marker whose expression levels correlated with poorer metastasis-free survival (MFS) (HR 5.4;
P
< .001) and poorer overall survival (OS) (HR 3.8;
P
< .001). Msi-1 silencing significantly inhibited proliferation ability and attenuated the migration and invasion activity of colon cancer cells.
Conclusions
Our study provides the basis to explore the use of Msi-1 as a novel prognostic biomarker in colon cancer patients. Aberrant overexpression of Msi-1 during metastasis of colon cancer also suggests that it is a potential therapeutic target.</abstract><cop>New York</cop><pub>Springer-Verlag</pub><pmid>21442350</pmid><doi>10.1245/s10434-011-1567-9</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1068-9265 |
| ispartof | Annals of surgical oncology, 2011-07, Vol.18 (7), p.2074-2083 |
| issn | 1068-9265 1534-4681 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Adult Aged Aged, 80 and over Blotting, Western Cell Adhesion Cell Differentiation Cell Movement Cell Proliferation Colon - metabolism Colon - pathology Colonic Neoplasms - metabolism Colonic Neoplasms - mortality Colonic Neoplasms - pathology Female Follow-Up Studies Humans Immunoenzyme Techniques Lymphatic Metastasis Male Medicine Medicine & Public Health Middle Aged Nerve Tissue Proteins - antagonists & inhibitors Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Oncology Prognosis Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Small Interfering - genetics RNA-Binding Proteins - antagonists & inhibitors RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism Surgery Surgical Oncology Survival Rate Tissue Array Analysis Translational Research and Biomarkers Tumor Cells, Cultured Young Adult |
| title | Msi-1 is a Predictor of Survival and a Novel Therapeutic Target in Colon Cancer |
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