Novel methodology utilizing confocal laser scanning microscopy for systematic analysis in arthropods (Insecta)
The use of confocal laser scanning microscopy (CLSM) for imaging arthropod structures has the potential to profoundly impact the systematics of this group. Three-dimensional visualization of CLSM data provides high-fidelity, detailed images of minuscule structures unobtainable by traditional methods...
Gespeichert in:
| Veröffentlicht in: | Integrative and comparative biology 2006-04, Vol.46 (2), p.207-214 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 214 |
|---|---|
| container_issue | 2 |
| container_start_page | 207 |
| container_title | Integrative and comparative biology |
| container_volume | 46 |
| creator | Klaus, Angela V. Schawaroch, Valerie |
| description | The use of confocal laser scanning microscopy (CLSM) for imaging arthropod structures has the potential to profoundly impact the systematics of this group. Three-dimensional visualization of CLSM data provides high-fidelity, detailed images of minuscule structures unobtainable by traditional methods (for example, hand illustration, bright-field light microscopy, scanning electron microscopy). A CLSM data set consists of a stack of 2-D images (“optical slices”) collected from a transparent, fluorescent specimen of suitable thickness. Small arthropod structures are particularly well suited for CLSM imaging owing to the autofluorescent nature of their tissues. Here, we document the practical aspects of a methodology developed for obtaining image stacks via CLSM from autofluorescent insect cuticular structures. |
| doi_str_mv | 10.1093/icb/icj015 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_872125244</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>3884792</jstor_id><sourcerecordid>3884792</sourcerecordid><originalsourceid>FETCH-LOGICAL-c439t-4d47d4cc45a6df4e16140e802061b96c94b484c345f19bebc438b90846dc0293</originalsourceid><addsrcrecordid>eNp9kctv1DAQxi0Eog-4cEbI4lAeUsBvx8eqYtmKFVx6QL1YjuO0XpJ4sR3U8NfjVdo9cOhh5NF8vxmN5wPgFUafMFL0s7dNiS3C_Ak4xpzLSiJCn-5zhkou6BE4SWmLCsERfg6OCBaSSMqPwfg9_HE9HFy-DW3ow80Mp-x7_9ePN9CGsQvW9LA3yUWYrBnHfX3wNoZkw26GXSj1OWU3mOwtNKPp5-QT9CM0Md_GsAttgu8vx-RsNh9egGed6ZN7ef-egqvVl6uLdbX58fXy4nxTWUZVrljLZMusZdyItmMOC8yQqxFBAjdKWMUaVjNLGe-walxTuupGoZqJ1iKi6Cl4t4zdxfB7cinrwSfr-t6MLkxJ15JgwgljhTx7lCSoLCQFKuDb_8BtmGL5bmEwl7XClBfo4wLt75Oi6_Qu-sHEWWOk917p4pVevCrwm_uJUzO49oA-mFOA1wuwTTnEg07rmklFilwtsi_nvzvIJv7SQlLJ9frntRb1ar3afBP6mv4DkQKpeQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>215789135</pqid></control><display><type>article</type><title>Novel methodology utilizing confocal laser scanning microscopy for systematic analysis in arthropods (Insecta)</title><source>Jstor Complete Legacy</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Klaus, Angela V. ; Schawaroch, Valerie</creator><creatorcontrib>Klaus, Angela V. ; Schawaroch, Valerie</creatorcontrib><description>The use of confocal laser scanning microscopy (CLSM) for imaging arthropod structures has the potential to profoundly impact the systematics of this group. Three-dimensional visualization of CLSM data provides high-fidelity, detailed images of minuscule structures unobtainable by traditional methods (for example, hand illustration, bright-field light microscopy, scanning electron microscopy). A CLSM data set consists of a stack of 2-D images (“optical slices”) collected from a transparent, fluorescent specimen of suitable thickness. Small arthropod structures are particularly well suited for CLSM imaging owing to the autofluorescent nature of their tissues. Here, we document the practical aspects of a methodology developed for obtaining image stacks via CLSM from autofluorescent insect cuticular structures.</description><identifier>ISSN: 1540-7063</identifier><identifier>EISSN: 1557-7023</identifier><identifier>DOI: 10.1093/icb/icj015</identifier><identifier>PMID: 21672735</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Arthropoda ; Arthropods ; Biology ; Data analysis ; Data loss ; Data visualization ; Datasets ; Imaging ; Insecta ; Lasers ; Microscopy ; Research methodology ; Specimens ; The New Microscopy: Toward a Phylogenetic Synthesis ; Three dimensional imaging</subject><ispartof>Integrative and comparative biology, 2006-04, Vol.46 (2), p.207-214</ispartof><rights>Copyright 2006 The Society for Integrative and Comparative Biology</rights><rights>Copyright Society for Integrative and Comparative Biology Apr 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-4d47d4cc45a6df4e16140e802061b96c94b484c345f19bebc438b90846dc0293</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3884792$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3884792$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21672735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Klaus, Angela V.</creatorcontrib><creatorcontrib>Schawaroch, Valerie</creatorcontrib><title>Novel methodology utilizing confocal laser scanning microscopy for systematic analysis in arthropods (Insecta)</title><title>Integrative and comparative biology</title><addtitle>Integr Comp Biol</addtitle><description>The use of confocal laser scanning microscopy (CLSM) for imaging arthropod structures has the potential to profoundly impact the systematics of this group. Three-dimensional visualization of CLSM data provides high-fidelity, detailed images of minuscule structures unobtainable by traditional methods (for example, hand illustration, bright-field light microscopy, scanning electron microscopy). A CLSM data set consists of a stack of 2-D images (“optical slices”) collected from a transparent, fluorescent specimen of suitable thickness. Small arthropod structures are particularly well suited for CLSM imaging owing to the autofluorescent nature of their tissues. Here, we document the practical aspects of a methodology developed for obtaining image stacks via CLSM from autofluorescent insect cuticular structures.</description><subject>Arthropoda</subject><subject>Arthropods</subject><subject>Biology</subject><subject>Data analysis</subject><subject>Data loss</subject><subject>Data visualization</subject><subject>Datasets</subject><subject>Imaging</subject><subject>Insecta</subject><subject>Lasers</subject><subject>Microscopy</subject><subject>Research methodology</subject><subject>Specimens</subject><subject>The New Microscopy: Toward a Phylogenetic Synthesis</subject><subject>Three dimensional imaging</subject><issn>1540-7063</issn><issn>1557-7023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNp9kctv1DAQxi0Eog-4cEbI4lAeUsBvx8eqYtmKFVx6QL1YjuO0XpJ4sR3U8NfjVdo9cOhh5NF8vxmN5wPgFUafMFL0s7dNiS3C_Ak4xpzLSiJCn-5zhkou6BE4SWmLCsERfg6OCBaSSMqPwfg9_HE9HFy-DW3ow80Mp-x7_9ePN9CGsQvW9LA3yUWYrBnHfX3wNoZkw26GXSj1OWU3mOwtNKPp5-QT9CM0Md_GsAttgu8vx-RsNh9egGed6ZN7ef-egqvVl6uLdbX58fXy4nxTWUZVrljLZMusZdyItmMOC8yQqxFBAjdKWMUaVjNLGe-walxTuupGoZqJ1iKi6Cl4t4zdxfB7cinrwSfr-t6MLkxJ15JgwgljhTx7lCSoLCQFKuDb_8BtmGL5bmEwl7XClBfo4wLt75Oi6_Qu-sHEWWOk917p4pVevCrwm_uJUzO49oA-mFOA1wuwTTnEg07rmklFilwtsi_nvzvIJv7SQlLJ9frntRb1ar3afBP6mv4DkQKpeQ</recordid><startdate>20060401</startdate><enddate>20060401</enddate><creator>Klaus, Angela V.</creator><creator>Schawaroch, Valerie</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20060401</creationdate><title>Novel methodology utilizing confocal laser scanning microscopy for systematic analysis in arthropods (Insecta)</title><author>Klaus, Angela V. ; Schawaroch, Valerie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-4d47d4cc45a6df4e16140e802061b96c94b484c345f19bebc438b90846dc0293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Arthropoda</topic><topic>Arthropods</topic><topic>Biology</topic><topic>Data analysis</topic><topic>Data loss</topic><topic>Data visualization</topic><topic>Datasets</topic><topic>Imaging</topic><topic>Insecta</topic><topic>Lasers</topic><topic>Microscopy</topic><topic>Research methodology</topic><topic>Specimens</topic><topic>The New Microscopy: Toward a Phylogenetic Synthesis</topic><topic>Three dimensional imaging</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Klaus, Angela V.</creatorcontrib><creatorcontrib>Schawaroch, Valerie</creatorcontrib><collection>Istex</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Integrative and comparative biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Klaus, Angela V.</au><au>Schawaroch, Valerie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel methodology utilizing confocal laser scanning microscopy for systematic analysis in arthropods (Insecta)</atitle><jtitle>Integrative and comparative biology</jtitle><addtitle>Integr Comp Biol</addtitle><date>2006-04-01</date><risdate>2006</risdate><volume>46</volume><issue>2</issue><spage>207</spage><epage>214</epage><pages>207-214</pages><issn>1540-7063</issn><eissn>1557-7023</eissn><abstract>The use of confocal laser scanning microscopy (CLSM) for imaging arthropod structures has the potential to profoundly impact the systematics of this group. Three-dimensional visualization of CLSM data provides high-fidelity, detailed images of minuscule structures unobtainable by traditional methods (for example, hand illustration, bright-field light microscopy, scanning electron microscopy). A CLSM data set consists of a stack of 2-D images (“optical slices”) collected from a transparent, fluorescent specimen of suitable thickness. Small arthropod structures are particularly well suited for CLSM imaging owing to the autofluorescent nature of their tissues. Here, we document the practical aspects of a methodology developed for obtaining image stacks via CLSM from autofluorescent insect cuticular structures.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>21672735</pmid><doi>10.1093/icb/icj015</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1540-7063 |
| ispartof | Integrative and comparative biology, 2006-04, Vol.46 (2), p.207-214 |
| issn | 1540-7063 1557-7023 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_872125244 |
| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Arthropoda Arthropods Biology Data analysis Data loss Data visualization Datasets Imaging Insecta Lasers Microscopy Research methodology Specimens The New Microscopy: Toward a Phylogenetic Synthesis Three dimensional imaging |
| title | Novel methodology utilizing confocal laser scanning microscopy for systematic analysis in arthropods (Insecta) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T09%3A34%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Novel%20methodology%20utilizing%20confocal%20laser%20scanning%20microscopy%20for%20systematic%20analysis%20in%20arthropods%20(Insecta)&rft.jtitle=Integrative%20and%20comparative%20biology&rft.au=Klaus,%20Angela%20V.&rft.date=2006-04-01&rft.volume=46&rft.issue=2&rft.spage=207&rft.epage=214&rft.pages=207-214&rft.issn=1540-7063&rft.eissn=1557-7023&rft_id=info:doi/10.1093/icb/icj015&rft_dat=%3Cjstor_proqu%3E3884792%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=215789135&rft_id=info:pmid/21672735&rft_jstor_id=3884792&rfr_iscdi=true |