Real-time PCR of single bacterial cells on an array of adhering droplets

Real-time PCR at the single bacterial cell level is an indispensable tool to quantitatively reveal the heterogeneity of isogenetic cells. Conventional PCR platforms that utilize microtiter plates or PCR tubes have been widely used, but their large reaction volumes are not suited for sensitive single...

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Veröffentlicht in:	Lab on a chip 2011-07, Vol.11 (13), p.2276-2281
Hauptverfasser:	Shi, Xu, Lin, Liang-I, Chen, Szu-yu, Chao, Shih-Hui, Zhang, Weiwen, Meldrum, Deirdre R
Format:	Artikel
Sprache:	eng
Schlagworte:	Adhesives Bacteria Genetic analysis Polymerase Chain Reaction - instrumentation Reproducibility of Results Single-Cell Analysis - instrumentation Synechocystis - cytology Synechocystis - genetics Temperature Time Factors
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