Raman Spectroscopic Study of Aggregation Process of Antibiotic Amphotericin B Induced by H+, Na+, and K+ Ions
The normal and the preresonance Raman effects (NR and PRR) of spectroscopy have been used to monitor and explain the aggregation processes of amphotericin B (AmB) in aqueous solution at different pH values and containing the K+ and Na+ ions. The resonance-enhanced and normal vibrational Raman spectr...
Gespeichert in:
Veröffentlicht in: | The journal of physical chemistry. B 2011-05, Vol.115 (17), p.5032-5036 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The normal and the preresonance Raman effects (NR and PRR) of spectroscopy have been used to monitor and explain the aggregation processes of amphotericin B (AmB) in aqueous solution at different pH values and containing the K+ and Na+ ions. The resonance-enhanced and normal vibrational Raman spectra were recorded with a semiconductor laser (ex 785 nm) and an argon laser (ex 514.5 nm) for investigation of interactions between AmB chromophores. The essential difference between the samples stimulated by resonance-enhanced and by near-infrared was in the CC stretching mode region of polyene chain. The processes connected with the aggregation of AmB led to changes in the chromophore, which were only visible as a remarkable broadening of the band centered at 1558 cm−1. The understanding of possible physical mechanisms responsible for the molecular aggregation of the drug is important from the pharmaceutical applicability standpoint. |
---|---|
ISSN: | 1520-6106 1520-5207 |
DOI: | 10.1021/jp201755s |