cAMP activates hyperpolarization-activated Ca2+ channels in the pollen of Pyrus pyrifolia
Many signal-transduction processes in plant cells have been suggested to be triggered by signal-induced opening of calcium ion (Ca 2+ ) channels in the plasma membrane. Cyclic nucleotides have been proposed to lead to an increase in cytosolic free Ca 2+ in pollen. However, direct recordings of cycli...
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| Veröffentlicht in: | Plant cell reports 2011-07, Vol.30 (7), p.1193-1200 |
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| creator | Wu, Juyou Qu, Haiyong Jin, Cong Shang, Zhongling Wu, Jun Xu, Guohua Gao, Yongbing Zhang, Shaoling |
| description | Many signal-transduction processes in plant cells have been suggested to be triggered by signal-induced opening of calcium ion (Ca
2+
) channels in the plasma membrane. Cyclic nucleotides have been proposed to lead to an increase in cytosolic free Ca
2+
in pollen. However, direct recordings of cyclic-nucleotide-induced Ca
2+
currents in pollen have not yet been obtained. Here, we report that cyclic AMP (cAMP) activated a hyperpolarization-activated Ca
2+
channel in the
Pyrus pyrifolia
pollen tube using the patch-clamp technique, which resulted in a significant increase in pollen tube protoplast cytosolic-Ca
2+
concentration. Outside-out single channel configuration identified that cAMP directly increased the Ca
2+
channel open-probability without affecting channel conductance. cAMP-induced currents were composed of both Ca
2+
and K
+
. However, cGMP failed to mimic the cAMP effect. Higher cytosolic free-Ca
2+
concentration significantly decreased the cAMP-induced currents. These results provide direct evidence for cAMP activation of hyperpolarization-activated Ca
2+
channels in the plasma membrane of pollen tubes, which, in turn, modulate cellular responses in regulation of pollen tube growth. |
| doi_str_mv | 10.1007/s00299-011-1027-9 |
| format | Article |
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2+
) channels in the plasma membrane. Cyclic nucleotides have been proposed to lead to an increase in cytosolic free Ca
2+
in pollen. However, direct recordings of cyclic-nucleotide-induced Ca
2+
currents in pollen have not yet been obtained. Here, we report that cyclic AMP (cAMP) activated a hyperpolarization-activated Ca
2+
channel in the
Pyrus pyrifolia
pollen tube using the patch-clamp technique, which resulted in a significant increase in pollen tube protoplast cytosolic-Ca
2+
concentration. Outside-out single channel configuration identified that cAMP directly increased the Ca
2+
channel open-probability without affecting channel conductance. cAMP-induced currents were composed of both Ca
2+
and K
+
. However, cGMP failed to mimic the cAMP effect. Higher cytosolic free-Ca
2+
concentration significantly decreased the cAMP-induced currents. These results provide direct evidence for cAMP activation of hyperpolarization-activated Ca
2+
channels in the plasma membrane of pollen tubes, which, in turn, modulate cellular responses in regulation of pollen tube growth.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-011-1027-9</identifier><identifier>PMID: 21331543</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Alloxan - pharmacology ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Calcium - metabolism ; Calcium Channels - drug effects ; Calcium Channels - metabolism ; Calcium Signaling ; Cell Biology ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Cell Membrane Permeability ; Cell Polarity ; Cyclic AMP - metabolism ; Fundamental and applied biological sciences. Psychology ; Life Sciences ; Membrane Potentials ; Original Paper ; Patch-Clamp Techniques ; Plant Biochemistry ; Plant Sciences ; Pollen Tube - drug effects ; Pollen Tube - growth & development ; Pollen Tube - metabolism ; Protoplasts - drug effects ; Protoplasts - metabolism ; Pyrus - drug effects ; Pyrus - growth & development ; Pyrus - metabolism</subject><ispartof>Plant cell reports, 2011-07, Vol.30 (7), p.1193-1200</ispartof><rights>Springer-Verlag 2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-d9f0dc5e2f7a1698690e405a9309df95ffcc172e93ce3c9737d7e7afcaae87c23</citedby><cites>FETCH-LOGICAL-c369t-d9f0dc5e2f7a1698690e405a9309df95ffcc172e93ce3c9737d7e7afcaae87c23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00299-011-1027-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00299-011-1027-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24290246$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21331543$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Juyou</creatorcontrib><creatorcontrib>Qu, Haiyong</creatorcontrib><creatorcontrib>Jin, Cong</creatorcontrib><creatorcontrib>Shang, Zhongling</creatorcontrib><creatorcontrib>Wu, Jun</creatorcontrib><creatorcontrib>Xu, Guohua</creatorcontrib><creatorcontrib>Gao, Yongbing</creatorcontrib><creatorcontrib>Zhang, Shaoling</creatorcontrib><title>cAMP activates hyperpolarization-activated Ca2+ channels in the pollen of Pyrus pyrifolia</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><addtitle>Plant Cell Rep</addtitle><description>Many signal-transduction processes in plant cells have been suggested to be triggered by signal-induced opening of calcium ion (Ca
2+
) channels in the plasma membrane. Cyclic nucleotides have been proposed to lead to an increase in cytosolic free Ca
2+
in pollen. However, direct recordings of cyclic-nucleotide-induced Ca
2+
currents in pollen have not yet been obtained. Here, we report that cyclic AMP (cAMP) activated a hyperpolarization-activated Ca
2+
channel in the
Pyrus pyrifolia
pollen tube using the patch-clamp technique, which resulted in a significant increase in pollen tube protoplast cytosolic-Ca
2+
concentration. Outside-out single channel configuration identified that cAMP directly increased the Ca
2+
channel open-probability without affecting channel conductance. cAMP-induced currents were composed of both Ca
2+
and K
+
. However, cGMP failed to mimic the cAMP effect. Higher cytosolic free-Ca
2+
concentration significantly decreased the cAMP-induced currents. These results provide direct evidence for cAMP activation of hyperpolarization-activated Ca
2+
channels in the plasma membrane of pollen tubes, which, in turn, modulate cellular responses in regulation of pollen tube growth.</description><subject>Alloxan - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Calcium - metabolism</subject><subject>Calcium Channels - drug effects</subject><subject>Calcium Channels - metabolism</subject><subject>Calcium Signaling</subject><subject>Cell Biology</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Membrane Permeability</subject><subject>Cell Polarity</subject><subject>Cyclic AMP - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Life Sciences</subject><subject>Membrane Potentials</subject><subject>Original Paper</subject><subject>Patch-Clamp Techniques</subject><subject>Plant Biochemistry</subject><subject>Plant Sciences</subject><subject>Pollen Tube - drug effects</subject><subject>Pollen Tube - growth & development</subject><subject>Pollen Tube - metabolism</subject><subject>Protoplasts - drug effects</subject><subject>Protoplasts - metabolism</subject><subject>Pyrus - drug effects</subject><subject>Pyrus - growth & development</subject><subject>Pyrus - metabolism</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFPGzEQha0KVFLaH9BL5QviUJmO7c06PqKIFiQQHFqpPVlT75gYbbxbexcp_PpulITeeprD-94b6WPso4QLCWC-FABlrQAphQRlhH3DZrLSSijQP4_YDIySwhhZnbB3pTwBTKGp37ITJbWW80rP2C9_effA0Q_xGQcqfLXpKfddizm-4BC7JA5Zw5eoPnO_wpSoLTwmPqyIT2xLiXeBP2zyWHi_yTF0bcT37DhgW-jD_p6yH1-vvi-vxe39t5vl5a3wuraDaGyAxs9JBYOytovaAlUwR6vBNsHOQ_BeGkVWe9LeGm0aQwaDR6SF8UqfsvPdbp-7PyOVwa1j8dS2mKgbi1sYaWu9ADuRckf63JWSKbg-xzXmjZPgtkLdTqibhLqtULftfNqvj7_X1Lw2DgYn4GwPYPHYhozJx_KPq5QFVdUTp3ZcmaL0SNk9dWNOk5n_fP8Lq7aOfA</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Wu, Juyou</creator><creator>Qu, Haiyong</creator><creator>Jin, Cong</creator><creator>Shang, Zhongling</creator><creator>Wu, Jun</creator><creator>Xu, Guohua</creator><creator>Gao, Yongbing</creator><creator>Zhang, Shaoling</creator><general>Springer-Verlag</general><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110701</creationdate><title>cAMP activates hyperpolarization-activated Ca2+ channels in the pollen of Pyrus pyrifolia</title><author>Wu, Juyou ; Qu, Haiyong ; Jin, Cong ; Shang, Zhongling ; Wu, Jun ; Xu, Guohua ; Gao, Yongbing ; Zhang, Shaoling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-d9f0dc5e2f7a1698690e405a9309df95ffcc172e93ce3c9737d7e7afcaae87c23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Alloxan - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Calcium - metabolism</topic><topic>Calcium Channels - drug effects</topic><topic>Calcium Channels - metabolism</topic><topic>Calcium Signaling</topic><topic>Cell Biology</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Membrane Permeability</topic><topic>Cell Polarity</topic><topic>Cyclic AMP - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Life Sciences</topic><topic>Membrane Potentials</topic><topic>Original Paper</topic><topic>Patch-Clamp Techniques</topic><topic>Plant Biochemistry</topic><topic>Plant Sciences</topic><topic>Pollen Tube - drug effects</topic><topic>Pollen Tube - growth & development</topic><topic>Pollen Tube - metabolism</topic><topic>Protoplasts - drug effects</topic><topic>Protoplasts - metabolism</topic><topic>Pyrus - drug effects</topic><topic>Pyrus - growth & development</topic><topic>Pyrus - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Juyou</creatorcontrib><creatorcontrib>Qu, Haiyong</creatorcontrib><creatorcontrib>Jin, Cong</creatorcontrib><creatorcontrib>Shang, Zhongling</creatorcontrib><creatorcontrib>Wu, Jun</creatorcontrib><creatorcontrib>Xu, Guohua</creatorcontrib><creatorcontrib>Gao, Yongbing</creatorcontrib><creatorcontrib>Zhang, Shaoling</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Juyou</au><au>Qu, Haiyong</au><au>Jin, Cong</au><au>Shang, Zhongling</au><au>Wu, Jun</au><au>Xu, Guohua</au><au>Gao, Yongbing</au><au>Zhang, Shaoling</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>cAMP activates hyperpolarization-activated Ca2+ channels in the pollen of Pyrus pyrifolia</atitle><jtitle>Plant cell reports</jtitle><stitle>Plant Cell Rep</stitle><addtitle>Plant Cell Rep</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>30</volume><issue>7</issue><spage>1193</spage><epage>1200</epage><pages>1193-1200</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>Many signal-transduction processes in plant cells have been suggested to be triggered by signal-induced opening of calcium ion (Ca
2+
) channels in the plasma membrane. Cyclic nucleotides have been proposed to lead to an increase in cytosolic free Ca
2+
in pollen. However, direct recordings of cyclic-nucleotide-induced Ca
2+
currents in pollen have not yet been obtained. Here, we report that cyclic AMP (cAMP) activated a hyperpolarization-activated Ca
2+
channel in the
Pyrus pyrifolia
pollen tube using the patch-clamp technique, which resulted in a significant increase in pollen tube protoplast cytosolic-Ca
2+
concentration. Outside-out single channel configuration identified that cAMP directly increased the Ca
2+
channel open-probability without affecting channel conductance. cAMP-induced currents were composed of both Ca
2+
and K
+
. However, cGMP failed to mimic the cAMP effect. Higher cytosolic free-Ca
2+
concentration significantly decreased the cAMP-induced currents. These results provide direct evidence for cAMP activation of hyperpolarization-activated Ca
2+
channels in the plasma membrane of pollen tubes, which, in turn, modulate cellular responses in regulation of pollen tube growth.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>21331543</pmid><doi>10.1007/s00299-011-1027-9</doi><tpages>8</tpages></addata></record> |
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| ispartof | Plant cell reports, 2011-07, Vol.30 (7), p.1193-1200 |
| issn | 0721-7714 1432-203X |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Alloxan - pharmacology Biological and medical sciences Biomedical and Life Sciences Biotechnology Calcium - metabolism Calcium Channels - drug effects Calcium Channels - metabolism Calcium Signaling Cell Biology Cell Membrane - drug effects Cell Membrane - metabolism Cell Membrane Permeability Cell Polarity Cyclic AMP - metabolism Fundamental and applied biological sciences. Psychology Life Sciences Membrane Potentials Original Paper Patch-Clamp Techniques Plant Biochemistry Plant Sciences Pollen Tube - drug effects Pollen Tube - growth & development Pollen Tube - metabolism Protoplasts - drug effects Protoplasts - metabolism Pyrus - drug effects Pyrus - growth & development Pyrus - metabolism |
| title | cAMP activates hyperpolarization-activated Ca2+ channels in the pollen of Pyrus pyrifolia |
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