Comparison of different PCR methods for detection of Brucella spp. in human blood samples

For detection of Brucella species by PCR four DNA extraction methods and four targets were compared using pure culture of Brucella melitensis and the best conditions were applied in clinical samples. It was found that the MagNA Pure LC method was the most efficient and sensitive method showing a pos...

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Veröffentlicht in:	Polish journal of microbiology 2011, Vol.60 (1), p.27-33
Hauptverfasser:	Al-Ajlan, Hisham H, Ibrahim, Abdelnasser S S, Al-Salamah, Ali A
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteremia - blood Bacteremia - microbiology Brucella - isolation & purification DNA, Bacterial - isolation & purification Humans Polymerase Chain Reaction - methods
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