Malignant pleural effusion cells show aberrant glucose metabolism gene expression
Malignant pleural effusion (MPE) accompanying lung adenocarcinoma indicates poor prognosis and early metastasis. This study aimed to identify genes related to MPE formation. Three tissue sample cohorts, seven from healthy lungs, 18 from stage I-III lung adenocarcinoma with adjacent healthy lung tiss...
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| Veröffentlicht in: | The European respiratory journal 2011-06, Vol.37 (6), p.1453-1465 |
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| creator | LIN, C.-C CHEN, L.-C TSENG, V. S YAN, J.-J LAI, W.-W SU, W.-P LIN, C.-H HUANG, C-Y. F SU, W.-C |
| description | Malignant pleural effusion (MPE) accompanying lung adenocarcinoma indicates poor prognosis and early metastasis. This study aimed to identify genes related to MPE formation. Three tissue sample cohorts, seven from healthy lungs, 18 from stage I-III lung adenocarcinoma with adjacent healthy lung tissue and 13 from lung adenocarcinomas with MPE, were analysed by oligonucleotide microarray. The identified genes were verified by quantitative real-time PCR (qRT-PCR), immunohistochemical staining, and immunofluorescence confocal microscopy. 20 up- or down-regulated genes with a two-fold change in MPE cancer cells compared to healthy tissues were differentially expressed from early- to late-stage lung cancer. Of 13 genes related to cellular metabolism, aldolase A (ALDOA), sorbitol dehydrogenase (SORD), transketolase (TKT), and tuberous sclerosis 1 (TSC1) were related to glucose metabolism. qRT-PCR validated their mRNA expressions in pleural metastatic samples. Immunohistochemical staining confirmed aberrant TKT, ALDOA, and TSC1 expressions in tumour cells. Immunofluorescence confirmed TKT co-localisation and co-distribution of ALDOA with thyroid transcription factor 1-positive cancer cells. TKT regulated the proliferation, vascular endothelial growth factor secretion in vitro and in vivo vascular permeability of cancer cell. Glucose metabolic reprogramming by ALDOA, SORD, TKT and TSC1 is important in MPE pathogenesis. |
| doi_str_mv | 10.1183/09031936.00015710 |
| format | Article |
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S ; YAN, J.-J ; LAI, W.-W ; SU, W.-P ; LIN, C.-H ; HUANG, C-Y. F ; SU, W.-C</creator><creatorcontrib>LIN, C.-C ; CHEN, L.-C ; TSENG, V. S ; YAN, J.-J ; LAI, W.-W ; SU, W.-P ; LIN, C.-H ; HUANG, C-Y. F ; SU, W.-C</creatorcontrib><description>Malignant pleural effusion (MPE) accompanying lung adenocarcinoma indicates poor prognosis and early metastasis. This study aimed to identify genes related to MPE formation. Three tissue sample cohorts, seven from healthy lungs, 18 from stage I-III lung adenocarcinoma with adjacent healthy lung tissue and 13 from lung adenocarcinomas with MPE, were analysed by oligonucleotide microarray. The identified genes were verified by quantitative real-time PCR (qRT-PCR), immunohistochemical staining, and immunofluorescence confocal microscopy. 20 up- or down-regulated genes with a two-fold change in MPE cancer cells compared to healthy tissues were differentially expressed from early- to late-stage lung cancer. Of 13 genes related to cellular metabolism, aldolase A (ALDOA), sorbitol dehydrogenase (SORD), transketolase (TKT), and tuberous sclerosis 1 (TSC1) were related to glucose metabolism. qRT-PCR validated their mRNA expressions in pleural metastatic samples. Immunohistochemical staining confirmed aberrant TKT, ALDOA, and TSC1 expressions in tumour cells. Immunofluorescence confirmed TKT co-localisation and co-distribution of ALDOA with thyroid transcription factor 1-positive cancer cells. TKT regulated the proliferation, vascular endothelial growth factor secretion in vitro and in vivo vascular permeability of cancer cell. Glucose metabolic reprogramming by ALDOA, SORD, TKT and TSC1 is important in MPE pathogenesis.</description><identifier>ISSN: 0903-1936</identifier><identifier>EISSN: 1399-3003</identifier><identifier>DOI: 10.1183/09031936.00015710</identifier><identifier>PMID: 20884743</identifier><language>eng</language><publisher>Leeds: Maney</publisher><subject>Adenocarcinoma - complications ; Adenocarcinoma of Lung ; Adult ; Aged ; Biological and medical sciences ; Capillary Permeability - genetics ; Cell Line, Tumor ; Cell Proliferation ; Cohort Studies ; Female ; Fructose-Bisphosphate Aldolase - genetics ; Gene Expression Regulation, Neoplastic ; Glucose - metabolism ; Humans ; L-Iditol 2-Dehydrogenase - genetics ; Lung Neoplasms - complications ; Medical sciences ; Middle Aged ; Nuclear Proteins - genetics ; Pleural Effusion, Malignant - genetics ; Pleural Effusion, Malignant - metabolism ; Pleural Effusion, Malignant - pathology ; Pneumology ; Thyroid Nuclear Factor 1 ; Transcription Factors - genetics ; Transketolase - genetics ; Tuberous Sclerosis Complex 1 Protein ; Tumor Suppressor Proteins - genetics ; Tumors of the respiratory system and mediastinum ; Vascular Endothelial Growth Factor A - metabolism</subject><ispartof>The European respiratory journal, 2011-06, Vol.37 (6), p.1453-1465</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-b478d6fbfe9ed1e14b24495f68d27be0d2085634f75d8de1742dc2cd58b222263</citedby><cites>FETCH-LOGICAL-c373t-b478d6fbfe9ed1e14b24495f68d27be0d2085634f75d8de1742dc2cd58b222263</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24227640$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20884743$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LIN, C.-C</creatorcontrib><creatorcontrib>CHEN, L.-C</creatorcontrib><creatorcontrib>TSENG, V. S</creatorcontrib><creatorcontrib>YAN, J.-J</creatorcontrib><creatorcontrib>LAI, W.-W</creatorcontrib><creatorcontrib>SU, W.-P</creatorcontrib><creatorcontrib>LIN, C.-H</creatorcontrib><creatorcontrib>HUANG, C-Y. F</creatorcontrib><creatorcontrib>SU, W.-C</creatorcontrib><title>Malignant pleural effusion cells show aberrant glucose metabolism gene expression</title><title>The European respiratory journal</title><addtitle>Eur Respir J</addtitle><description>Malignant pleural effusion (MPE) accompanying lung adenocarcinoma indicates poor prognosis and early metastasis. This study aimed to identify genes related to MPE formation. Three tissue sample cohorts, seven from healthy lungs, 18 from stage I-III lung adenocarcinoma with adjacent healthy lung tissue and 13 from lung adenocarcinomas with MPE, were analysed by oligonucleotide microarray. The identified genes were verified by quantitative real-time PCR (qRT-PCR), immunohistochemical staining, and immunofluorescence confocal microscopy. 20 up- or down-regulated genes with a two-fold change in MPE cancer cells compared to healthy tissues were differentially expressed from early- to late-stage lung cancer. Of 13 genes related to cellular metabolism, aldolase A (ALDOA), sorbitol dehydrogenase (SORD), transketolase (TKT), and tuberous sclerosis 1 (TSC1) were related to glucose metabolism. qRT-PCR validated their mRNA expressions in pleural metastatic samples. Immunohistochemical staining confirmed aberrant TKT, ALDOA, and TSC1 expressions in tumour cells. Immunofluorescence confirmed TKT co-localisation and co-distribution of ALDOA with thyroid transcription factor 1-positive cancer cells. TKT regulated the proliferation, vascular endothelial growth factor secretion in vitro and in vivo vascular permeability of cancer cell. Glucose metabolic reprogramming by ALDOA, SORD, TKT and TSC1 is important in MPE pathogenesis.</description><subject>Adenocarcinoma - complications</subject><subject>Adenocarcinoma of Lung</subject><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Capillary Permeability - genetics</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation</subject><subject>Cohort Studies</subject><subject>Female</subject><subject>Fructose-Bisphosphate Aldolase - genetics</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Glucose - metabolism</subject><subject>Humans</subject><subject>L-Iditol 2-Dehydrogenase - genetics</subject><subject>Lung Neoplasms - complications</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Nuclear Proteins - genetics</subject><subject>Pleural Effusion, Malignant - genetics</subject><subject>Pleural Effusion, Malignant - metabolism</subject><subject>Pleural Effusion, Malignant - pathology</subject><subject>Pneumology</subject><subject>Thyroid Nuclear Factor 1</subject><subject>Transcription Factors - genetics</subject><subject>Transketolase - genetics</subject><subject>Tuberous Sclerosis Complex 1 Protein</subject><subject>Tumor Suppressor Proteins - genetics</subject><subject>Tumors of the respiratory system and mediastinum</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><issn>0903-1936</issn><issn>1399-3003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMtKw0AUhgdRbK0-gBvJRlylzi2ZyVKKN6iIoOswlzM1Mrk4k6C-vQlt9WzO4nz_z-FD6JzgJSGSXeMCM1KwfIkxJpkg-ADNCSuKlGHMDtF8uqcTMEMnMX6MUM4ZOUYziqXkgrM5enlSvto0qumTzsMQlE_AuSFWbZMY8D4m8b39SpSGECZo4wfTRkhq6JVufRXrZAMNJPDdBYhT7BQdOeUjnO32Ar3d3b6uHtL18_3j6madGiZYn2oupM2ddlCAJUC4ppwXmculpUIDtuOPWc64E5mVFojg1BpqbCY1HSdnC3S17e1C-zlA7Mu6itPLqoF2iKUUmBZZLvlIki1pQhtjAFd2oapV-CkJLieR5V5kuRc5Zi527YOuwf4l9uZG4HIHqGiUd6MdU8V_jlMqco7ZLy_Sezo</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>LIN, C.-C</creator><creator>CHEN, L.-C</creator><creator>TSENG, V. S</creator><creator>YAN, J.-J</creator><creator>LAI, W.-W</creator><creator>SU, W.-P</creator><creator>LIN, C.-H</creator><creator>HUANG, C-Y. F</creator><creator>SU, W.-C</creator><general>Maney</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110601</creationdate><title>Malignant pleural effusion cells show aberrant glucose metabolism gene expression</title><author>LIN, C.-C ; CHEN, L.-C ; TSENG, V. S ; YAN, J.-J ; LAI, W.-W ; SU, W.-P ; LIN, C.-H ; HUANG, C-Y. F ; SU, W.-C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-b478d6fbfe9ed1e14b24495f68d27be0d2085634f75d8de1742dc2cd58b222263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adenocarcinoma - complications</topic><topic>Adenocarcinoma of Lung</topic><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Capillary Permeability - genetics</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation</topic><topic>Cohort Studies</topic><topic>Female</topic><topic>Fructose-Bisphosphate Aldolase - genetics</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Glucose - metabolism</topic><topic>Humans</topic><topic>L-Iditol 2-Dehydrogenase - genetics</topic><topic>Lung Neoplasms - complications</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Nuclear Proteins - genetics</topic><topic>Pleural Effusion, Malignant - genetics</topic><topic>Pleural Effusion, Malignant - metabolism</topic><topic>Pleural Effusion, Malignant - pathology</topic><topic>Pneumology</topic><topic>Thyroid Nuclear Factor 1</topic><topic>Transcription Factors - genetics</topic><topic>Transketolase - genetics</topic><topic>Tuberous Sclerosis Complex 1 Protein</topic><topic>Tumor Suppressor Proteins - genetics</topic><topic>Tumors of the respiratory system and mediastinum</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LIN, C.-C</creatorcontrib><creatorcontrib>CHEN, L.-C</creatorcontrib><creatorcontrib>TSENG, V. S</creatorcontrib><creatorcontrib>YAN, J.-J</creatorcontrib><creatorcontrib>LAI, W.-W</creatorcontrib><creatorcontrib>SU, W.-P</creatorcontrib><creatorcontrib>LIN, C.-H</creatorcontrib><creatorcontrib>HUANG, C-Y. F</creatorcontrib><creatorcontrib>SU, W.-C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The European respiratory journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LIN, C.-C</au><au>CHEN, L.-C</au><au>TSENG, V. S</au><au>YAN, J.-J</au><au>LAI, W.-W</au><au>SU, W.-P</au><au>LIN, C.-H</au><au>HUANG, C-Y. F</au><au>SU, W.-C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Malignant pleural effusion cells show aberrant glucose metabolism gene expression</atitle><jtitle>The European respiratory journal</jtitle><addtitle>Eur Respir J</addtitle><date>2011-06-01</date><risdate>2011</risdate><volume>37</volume><issue>6</issue><spage>1453</spage><epage>1465</epage><pages>1453-1465</pages><issn>0903-1936</issn><eissn>1399-3003</eissn><abstract>Malignant pleural effusion (MPE) accompanying lung adenocarcinoma indicates poor prognosis and early metastasis. This study aimed to identify genes related to MPE formation. Three tissue sample cohorts, seven from healthy lungs, 18 from stage I-III lung adenocarcinoma with adjacent healthy lung tissue and 13 from lung adenocarcinomas with MPE, were analysed by oligonucleotide microarray. The identified genes were verified by quantitative real-time PCR (qRT-PCR), immunohistochemical staining, and immunofluorescence confocal microscopy. 20 up- or down-regulated genes with a two-fold change in MPE cancer cells compared to healthy tissues were differentially expressed from early- to late-stage lung cancer. Of 13 genes related to cellular metabolism, aldolase A (ALDOA), sorbitol dehydrogenase (SORD), transketolase (TKT), and tuberous sclerosis 1 (TSC1) were related to glucose metabolism. qRT-PCR validated their mRNA expressions in pleural metastatic samples. Immunohistochemical staining confirmed aberrant TKT, ALDOA, and TSC1 expressions in tumour cells. Immunofluorescence confirmed TKT co-localisation and co-distribution of ALDOA with thyroid transcription factor 1-positive cancer cells. TKT regulated the proliferation, vascular endothelial growth factor secretion in vitro and in vivo vascular permeability of cancer cell. Glucose metabolic reprogramming by ALDOA, SORD, TKT and TSC1 is important in MPE pathogenesis.</abstract><cop>Leeds</cop><pub>Maney</pub><pmid>20884743</pmid><doi>10.1183/09031936.00015710</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenocarcinoma - complications Adenocarcinoma of Lung Adult Aged Biological and medical sciences Capillary Permeability - genetics Cell Line, Tumor Cell Proliferation Cohort Studies Female Fructose-Bisphosphate Aldolase - genetics Gene Expression Regulation, Neoplastic Glucose - metabolism Humans L-Iditol 2-Dehydrogenase - genetics Lung Neoplasms - complications Medical sciences Middle Aged Nuclear Proteins - genetics Pleural Effusion, Malignant - genetics Pleural Effusion, Malignant - metabolism Pleural Effusion, Malignant - pathology Pneumology Thyroid Nuclear Factor 1 Transcription Factors - genetics Transketolase - genetics Tuberous Sclerosis Complex 1 Protein Tumor Suppressor Proteins - genetics Tumors of the respiratory system and mediastinum Vascular Endothelial Growth Factor A - metabolism |
| title | Malignant pleural effusion cells show aberrant glucose metabolism gene expression |
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