Anaplasmosis in two dogs in France and molecular and phylogenetic characterization of Anaplasma phagocytophilum
: Two dogs in France were diagnosed with Anaplasma phagocytophilum infection by real‐time PCR. The most remarkable hematologic and biochemical findings were severe thrombocytopenia, mild neutrophilia, morulae in neutrophils, and increased serum concentration of the α2‐globulin fraction detected by a...
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| Veröffentlicht in: | Veterinary clinical pathology 2011-06, Vol.40 (2), p.215-221 |
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| creator | Canelas Domingos, Margarida Trotta, Michele Briend‐Marchal, Alexandra Medaille, Christine |
| description | : Two dogs in France were diagnosed with Anaplasma phagocytophilum infection by real‐time PCR. The most remarkable hematologic and biochemical findings were severe thrombocytopenia, mild neutrophilia, morulae in neutrophils, and increased serum concentration of the α2‐globulin fraction detected by agarose gel electrophoresis of serum proteins. Using sequencing of the partial 16S rRNA and ankA genes, molecular characterization of the A. phagocytophilum strains showed that the organisms from both dogs were identical to the European strains isolated from horses and people. Based on phylogenetic analysis, the ankA gene was more discriminating than the 16S rRNA gene in distinguishing the majority of European and American strains of A. phagocytophilum infecting people and animals. Three isolates of A. phagocytophilum, 1 from Spain (cow) and 2 from Norway (sheep and deer), were external to the European and American clades. |
| doi_str_mv | 10.1111/j.1939-165X.2011.00321.x |
| format | Article |
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The most remarkable hematologic and biochemical findings were severe thrombocytopenia, mild neutrophilia, morulae in neutrophils, and increased serum concentration of the α2‐globulin fraction detected by agarose gel electrophoresis of serum proteins. Using sequencing of the partial 16S rRNA and ankA genes, molecular characterization of the A. phagocytophilum strains showed that the organisms from both dogs were identical to the European strains isolated from horses and people. Based on phylogenetic analysis, the ankA gene was more discriminating than the 16S rRNA gene in distinguishing the majority of European and American strains of A. phagocytophilum infecting people and animals. 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The most remarkable hematologic and biochemical findings were severe thrombocytopenia, mild neutrophilia, morulae in neutrophils, and increased serum concentration of the α2‐globulin fraction detected by agarose gel electrophoresis of serum proteins. Using sequencing of the partial 16S rRNA and ankA genes, molecular characterization of the A. phagocytophilum strains showed that the organisms from both dogs were identical to the European strains isolated from horses and people. Based on phylogenetic analysis, the ankA gene was more discriminating than the 16S rRNA gene in distinguishing the majority of European and American strains of A. phagocytophilum infecting people and animals. Three isolates of A. phagocytophilum, 1 from Spain (cow) and 2 from Norway (sheep and deer), were external to the European and American clades.</description><subject>16S rRNA</subject><subject>Anaplasma phagocytophilum - genetics</subject><subject>Anaplasmosis - blood</subject><subject>Anaplasmosis - microbiology</subject><subject>Animals</subject><subject>AnkA gene</subject><subject>Base Sequence</subject><subject>Dog Diseases - blood</subject><subject>Dog Diseases - microbiology</subject><subject>Dogs</subject><subject>Female</subject><subject>Genes, Bacterial - genetics</subject><subject>granulocytic anaplasmosis</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>real‐time PCR</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Thrombocytopenia - microbiology</subject><subject>Thrombocytopenia - veterinary</subject><subject>tick‐borne</subject><issn>0275-6382</issn><issn>1939-165X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtOxCAUhonROOPlFQw7V609pZQ2cWMm3hITXahxRyilM0xoqdBmHJ9e5qJr2XAOfOcnfAhhSGII62oZQ0nKCHL6EacJQJwkJIX46wBN_y4O0TRJGY1yUqQTdOL9MkA0HB2jSQqUZoTBFNmbTvRG-NZ67bHu8LCyuLbzbX3nRCcVFl2NW2uUHI1w265frI2dq04NWmK5EE7IQTn9LQZtO2wb_JsqAirmVq4H2y-0GdszdNQI49X5fj9Fb3e3r7OH6On5_nF28xRJkjOISCXLgpUZsEoVNCsyVtOcsBxyVTJCGSNpA1KVlagTmdcpQEMrWmZF-BhkipFTdLnL7Z39HJUfeKu9VMaITtnR84IlaUlJngWy2JHSWe-danjvdCvcmkPCN7b5km-k8o1UvrHNt7b5Vxi92D8yVq2q_wZ_9QbgegestFHrfwfz99lLKMgPjE6O2Q</recordid><startdate>201106</startdate><enddate>201106</enddate><creator>Canelas Domingos, Margarida</creator><creator>Trotta, Michele</creator><creator>Briend‐Marchal, Alexandra</creator><creator>Medaille, Christine</creator><general>Blackwell Publishing Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201106</creationdate><title>Anaplasmosis in two dogs in France and molecular and phylogenetic characterization of Anaplasma phagocytophilum</title><author>Canelas Domingos, Margarida ; Trotta, Michele ; Briend‐Marchal, Alexandra ; Medaille, Christine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3671-3bc9879417be854847d5637616e97357732f1ce9bad0c6d211f5b594815514e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>16S rRNA</topic><topic>Anaplasma phagocytophilum - genetics</topic><topic>Anaplasmosis - blood</topic><topic>Anaplasmosis - microbiology</topic><topic>Animals</topic><topic>AnkA gene</topic><topic>Base Sequence</topic><topic>Dog Diseases - blood</topic><topic>Dog Diseases - microbiology</topic><topic>Dogs</topic><topic>Female</topic><topic>Genes, Bacterial - genetics</topic><topic>granulocytic anaplasmosis</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>real‐time PCR</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Thrombocytopenia - microbiology</topic><topic>Thrombocytopenia - veterinary</topic><topic>tick‐borne</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Canelas Domingos, Margarida</creatorcontrib><creatorcontrib>Trotta, Michele</creatorcontrib><creatorcontrib>Briend‐Marchal, Alexandra</creatorcontrib><creatorcontrib>Medaille, Christine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Canelas Domingos, Margarida</au><au>Trotta, Michele</au><au>Briend‐Marchal, Alexandra</au><au>Medaille, Christine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anaplasmosis in two dogs in France and molecular and phylogenetic characterization of Anaplasma phagocytophilum</atitle><jtitle>Veterinary clinical pathology</jtitle><addtitle>Vet Clin Pathol</addtitle><date>2011-06</date><risdate>2011</risdate><volume>40</volume><issue>2</issue><spage>215</spage><epage>221</epage><pages>215-221</pages><issn>0275-6382</issn><eissn>1939-165X</eissn><abstract>: Two dogs in France were diagnosed with Anaplasma phagocytophilum infection by real‐time PCR. 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Three isolates of A. phagocytophilum, 1 from Spain (cow) and 2 from Norway (sheep and deer), were external to the European and American clades.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>21554371</pmid><doi>10.1111/j.1939-165X.2011.00321.x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0275-6382 |
| ispartof | Veterinary clinical pathology, 2011-06, Vol.40 (2), p.215-221 |
| issn | 0275-6382 1939-165X |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | 16S rRNA Anaplasma phagocytophilum - genetics Anaplasmosis - blood Anaplasmosis - microbiology Animals AnkA gene Base Sequence Dog Diseases - blood Dog Diseases - microbiology Dogs Female Genes, Bacterial - genetics granulocytic anaplasmosis Male Molecular Sequence Data Phylogeny Polymerase Chain Reaction - veterinary real‐time PCR RNA, Ribosomal, 16S - genetics Thrombocytopenia - microbiology Thrombocytopenia - veterinary tick‐borne |
| title | Anaplasmosis in two dogs in France and molecular and phylogenetic characterization of Anaplasma phagocytophilum |
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