Large Stokes-Shift Fluorescent Silica Nanoparticles with Enhanced Emission Over Free Dye for Single Excitation Multiplexing
We describe a polycondensation reaction of silica precursors in a modified Stöber‐type basic ethanol solution to produce graded Stokes‐shift core‐shell silica nanoparticles providing bright and spectrally multiplexed sets of fluorescent tags that are excitable using a single excitation source. Dynam...
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| Veröffentlicht in: | Macromolecular rapid communications. 2009-11, Vol.30 (22), p.1907-1910 |
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| container_end_page | 1910 |
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| container_issue | 22 |
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| container_title | Macromolecular rapid communications. |
| container_volume | 30 |
| creator | Herz, Erik Burns, Andrew Bonner, Daniel Wiesner, Ulrich |
| description | We describe a polycondensation reaction of silica precursors in a modified Stöber‐type basic ethanol solution to produce graded Stokes‐shift core‐shell silica nanoparticles providing bright and spectrally multiplexed sets of fluorescent tags that are excitable using a single excitation source. Dynamic light scattering and scanning electron microscopy demonstrate particle sizes in the sub‐10 nm regime. Absorption matched emission comparisons between encapsulated and free dyes in aqueous solution reveal about an order of magnitude per‐dye brightness enhancements that are apparent by simply looking at the solutions under laser illumination conditions.
The formation of fluorescent coreshell silica nanoparticles with diameters |
| doi_str_mv | 10.1002/marc.200900389 |
| format | Article |
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The formation of fluorescent coreshell silica nanoparticles with diameters <10nm incorporating a series of graded, large stokes‐shift dyes that allow multiplexing with a single excitation source is reported. Significant per‐dye brightness enhancements are achieved through encapsulation, and are easily observed under laser illumination.</description><identifier>ISSN: 1022-1336</identifier><identifier>EISSN: 1521-3927</identifier><identifier>DOI: 10.1002/marc.200900389</identifier><identifier>PMID: 21638473</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Dyes ; Emission ; Ethyl alcohol ; Excitation ; fluorescence ; fluorescent nanoprobe ; Light scattering ; Multiplexing ; Nanoparticles ; silica ; Silicon dioxide</subject><ispartof>Macromolecular rapid communications., 2009-11, Vol.30 (22), p.1907-1910</ispartof><rights>Copyright © 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4149-30c53f40d4e6b9143e79a212277f3b30a2db66ec0b0c2ce5e1dcd9e711ec5bee3</citedby><cites>FETCH-LOGICAL-c4149-30c53f40d4e6b9143e79a212277f3b30a2db66ec0b0c2ce5e1dcd9e711ec5bee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmarc.200900389$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmarc.200900389$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21638473$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Herz, Erik</creatorcontrib><creatorcontrib>Burns, Andrew</creatorcontrib><creatorcontrib>Bonner, Daniel</creatorcontrib><creatorcontrib>Wiesner, Ulrich</creatorcontrib><title>Large Stokes-Shift Fluorescent Silica Nanoparticles with Enhanced Emission Over Free Dye for Single Excitation Multiplexing</title><title>Macromolecular rapid communications.</title><addtitle>Macromol. Rapid Commun</addtitle><description>We describe a polycondensation reaction of silica precursors in a modified Stöber‐type basic ethanol solution to produce graded Stokes‐shift core‐shell silica nanoparticles providing bright and spectrally multiplexed sets of fluorescent tags that are excitable using a single excitation source. Dynamic light scattering and scanning electron microscopy demonstrate particle sizes in the sub‐10 nm regime. Absorption matched emission comparisons between encapsulated and free dyes in aqueous solution reveal about an order of magnitude per‐dye brightness enhancements that are apparent by simply looking at the solutions under laser illumination conditions.
The formation of fluorescent coreshell silica nanoparticles with diameters <10nm incorporating a series of graded, large stokes‐shift dyes that allow multiplexing with a single excitation source is reported. Significant per‐dye brightness enhancements are achieved through encapsulation, and are easily observed under laser illumination.</description><subject>Dyes</subject><subject>Emission</subject><subject>Ethyl alcohol</subject><subject>Excitation</subject><subject>fluorescence</subject><subject>fluorescent nanoprobe</subject><subject>Light scattering</subject><subject>Multiplexing</subject><subject>Nanoparticles</subject><subject>silica</subject><subject>Silicon dioxide</subject><issn>1022-1336</issn><issn>1521-3927</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqFkU1vEzEQhi0Eoh9w5Yh847Tp2N5dx8cSkoKatBIp4mh5vbONqbMbbG-biD_PRikRt55mpHneVyM9hHxgMGIA_GJtgh1xAAUgxuoVOWUFZ5lQXL4eduA8Y0KUJ-Qsxl8AMM6BvyUnnJVinEtxSv7MTbhHukzdA8ZsuXJNojPfdwGjxTbRpfPOGnpj2m5jQnLWY6RPLq3otF2Z1mJNp2sXo-taevuIgc4CIv2yQ9p0YUi39x7pdGtdMmnPLHqf3Mbjdri8I28a4yO-f57n5Mdsejf5ms1vr75NLueZzVmuMgG2EE0OdY5lpVguUCrDGedSNqISYHhdlSVaqMByiwWy2tYKJWNoiwpRnJNPh95N6H73GJMePrbovWmx66MeS-CqYBJeJOWASsHLPTk6kDZ0MQZs9Ca4QcZOM9B7M3pvRh_NDIGPz9V9tcb6iP9TMQDqADw5j7sX6vTi8vvk__LskHUx4faYNeFBl1LIQv-8udJ3_HO5gGuppfgL4FCrGw</recordid><startdate>20091119</startdate><enddate>20091119</enddate><creator>Herz, Erik</creator><creator>Burns, Andrew</creator><creator>Bonner, Daniel</creator><creator>Wiesner, Ulrich</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20091119</creationdate><title>Large Stokes-Shift Fluorescent Silica Nanoparticles with Enhanced Emission Over Free Dye for Single Excitation Multiplexing</title><author>Herz, Erik ; Burns, Andrew ; Bonner, Daniel ; Wiesner, Ulrich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4149-30c53f40d4e6b9143e79a212277f3b30a2db66ec0b0c2ce5e1dcd9e711ec5bee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Dyes</topic><topic>Emission</topic><topic>Ethyl alcohol</topic><topic>Excitation</topic><topic>fluorescence</topic><topic>fluorescent nanoprobe</topic><topic>Light scattering</topic><topic>Multiplexing</topic><topic>Nanoparticles</topic><topic>silica</topic><topic>Silicon dioxide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Herz, Erik</creatorcontrib><creatorcontrib>Burns, Andrew</creatorcontrib><creatorcontrib>Bonner, Daniel</creatorcontrib><creatorcontrib>Wiesner, Ulrich</creatorcontrib><collection>Istex</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Macromolecular rapid communications.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Herz, Erik</au><au>Burns, Andrew</au><au>Bonner, Daniel</au><au>Wiesner, Ulrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Large Stokes-Shift Fluorescent Silica Nanoparticles with Enhanced Emission Over Free Dye for Single Excitation Multiplexing</atitle><jtitle>Macromolecular rapid communications.</jtitle><addtitle>Macromol. Rapid Commun</addtitle><date>2009-11-19</date><risdate>2009</risdate><volume>30</volume><issue>22</issue><spage>1907</spage><epage>1910</epage><pages>1907-1910</pages><issn>1022-1336</issn><eissn>1521-3927</eissn><abstract>We describe a polycondensation reaction of silica precursors in a modified Stöber‐type basic ethanol solution to produce graded Stokes‐shift core‐shell silica nanoparticles providing bright and spectrally multiplexed sets of fluorescent tags that are excitable using a single excitation source. Dynamic light scattering and scanning electron microscopy demonstrate particle sizes in the sub‐10 nm regime. Absorption matched emission comparisons between encapsulated and free dyes in aqueous solution reveal about an order of magnitude per‐dye brightness enhancements that are apparent by simply looking at the solutions under laser illumination conditions.
The formation of fluorescent coreshell silica nanoparticles with diameters <10nm incorporating a series of graded, large stokes‐shift dyes that allow multiplexing with a single excitation source is reported. Significant per‐dye brightness enhancements are achieved through encapsulation, and are easily observed under laser illumination.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>21638473</pmid><doi>10.1002/marc.200900389</doi><tpages>4</tpages></addata></record> |
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| ispartof | Macromolecular rapid communications., 2009-11, Vol.30 (22), p.1907-1910 |
| issn | 1022-1336 1521-3927 |
| language | eng |
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| source | Wiley Journals |
| subjects | Dyes Emission Ethyl alcohol Excitation fluorescence fluorescent nanoprobe Light scattering Multiplexing Nanoparticles silica Silicon dioxide |
| title | Large Stokes-Shift Fluorescent Silica Nanoparticles with Enhanced Emission Over Free Dye for Single Excitation Multiplexing |
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