Norepinephrine Enhances Radiosensitivity in Rat Ileal Epithelial Cells
We previously reported that the apoptosis index in jejunal crypt cells after X irradiation was greater in spontaneously hypertensive rats than in Wistar-Kyoto rats. Moreover, these same cells showed a suppression of apoptosis when reserpine was administered to induce sympathetic dysfunction in spont...
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| creator | Matsuu-Matsuyama, Mutsumi Okaichi, Kumio Shichijo, Kazuko Nakayama, Toshiyuki Nakashima, Masahiro Sekine, Ichiro |
| description | We previously reported that the apoptosis index in jejunal crypt cells after X irradiation was greater in spontaneously hypertensive rats than in Wistar-Kyoto rats. Moreover, these same cells showed a suppression of apoptosis when reserpine was administered to induce sympathetic dysfunction in spontaneously hypertensive rats or Wistar-Kyoto rats1). Whether the hyperfunction of the sympathetic nervous system is involved in the high susceptibility of the jejunal crypt cells to radiation-induced apoptosis was the subject of this study. The effect of norepinephrine (NE) on cell survival was examined using the colony formation assay after X-ray irradiation of rat ileal epithelial cells (IEC-18). The addition of 1μM NE decreased the surviving fraction of cells irradiated with 6Gy from 37% to 8%. The radiosensitivity of IEC-18 cells was enhanced by the addition of 1μM of NE. The irradiation and treatment with NE also resulted in an increased cellular apoptotic rate. These results showing enhanced radiosensitivity of rat ileal epithelial cells by NE suggest that NE may be one of the factors which aggravate acute radiation injury in the intestine. [INTRODUCTION] Spontaneously hypertensive rats, originally derived from Wistar Kyoto rats, are a well-known model for studying essential hypertension in humans. |
| doi_str_mv | 10.1269/jrr.10155 |
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Moreover, these same cells showed a suppression of apoptosis when reserpine was administered to induce sympathetic dysfunction in spontaneously hypertensive rats or Wistar-Kyoto rats1). Whether the hyperfunction of the sympathetic nervous system is involved in the high susceptibility of the jejunal crypt cells to radiation-induced apoptosis was the subject of this study. The effect of norepinephrine (NE) on cell survival was examined using the colony formation assay after X-ray irradiation of rat ileal epithelial cells (IEC-18). The addition of 1μM NE decreased the surviving fraction of cells irradiated with 6Gy from 37% to 8%. The radiosensitivity of IEC-18 cells was enhanced by the addition of 1μM of NE. The irradiation and treatment with NE also resulted in an increased cellular apoptotic rate. These results showing enhanced radiosensitivity of rat ileal epithelial cells by NE suggest that NE may be one of the factors which aggravate acute radiation injury in the intestine. [INTRODUCTION] Spontaneously hypertensive rats, originally derived from Wistar Kyoto rats, are a well-known model for studying essential hypertension in humans.</description><identifier>ISSN: 0449-3060</identifier><identifier>EISSN: 1349-9157</identifier><identifier>DOI: 10.1269/jrr.10155</identifier><identifier>PMID: 21343673</identifier><language>eng</language><publisher>England: THE JAPAN RADIATION RESEARCH SOCIETY</publisher><subject>Animals ; Apoptosis ; Cell Survival - drug effects ; Cell Survival - radiation effects ; Cells, Cultured ; Dose-Response Relationship, Radiation ; Hypertension ; Ileum - drug effects ; Ileum - physiology ; Ileum - radiation effects ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - physiology ; Intestinal Mucosa - radiation effects ; Norepinephrine ; Norepinephrine - pharmacology ; Radiation (Physics) ; Radiation Dosage ; Radiation Tolerance - drug effects ; Radiation Tolerance - physiology ; Radiation-Sensitizing Agents - pharmacology ; Rats ; Rats, Inbred WKY</subject><ispartof>JOURNAL OF RADIATION RESEARCH, 2011, Vol.52 (3), p.369-373</ispartof><rights>Copyright © 2011 by Journal of Radiation Research Editorial Committee 2011</rights><rights>COPYRIGHT 2011 Oxford University Press</rights><rights>Copyright Japan Science and Technology Agency 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c658t-43fd9bafa57b2a8f35fc013522e3501634bf2185dd7c56981b1a8f93cde022643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21343673$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsuu-Matsuyama, Mutsumi</creatorcontrib><creatorcontrib>Okaichi, Kumio</creatorcontrib><creatorcontrib>Shichijo, Kazuko</creatorcontrib><creatorcontrib>Nakayama, Toshiyuki</creatorcontrib><creatorcontrib>Nakashima, Masahiro</creatorcontrib><creatorcontrib>Sekine, Ichiro</creatorcontrib><creatorcontrib>Department of Tumor and Diagnostic Pathology</creatorcontrib><creatorcontrib>Department of Pathology</creatorcontrib><creatorcontrib>Tissue and Histopathology Section</creatorcontrib><creatorcontrib>Nagasaki Red Cross Blood Center</creatorcontrib><creatorcontrib>Department of Radiation Biophysics</creatorcontrib><creatorcontrib>Nagasaki University Graduate School of Biomedical Sciences</creatorcontrib><creatorcontrib>Atomic Bomb Disease Institute</creatorcontrib><title>Norepinephrine Enhances Radiosensitivity in Rat Ileal Epithelial Cells</title><title>JOURNAL OF RADIATION RESEARCH</title><addtitle>J Radiat Res</addtitle><description>We previously reported that the apoptosis index in jejunal crypt cells after X irradiation was greater in spontaneously hypertensive rats than in Wistar-Kyoto rats. Moreover, these same cells showed a suppression of apoptosis when reserpine was administered to induce sympathetic dysfunction in spontaneously hypertensive rats or Wistar-Kyoto rats1). Whether the hyperfunction of the sympathetic nervous system is involved in the high susceptibility of the jejunal crypt cells to radiation-induced apoptosis was the subject of this study. The effect of norepinephrine (NE) on cell survival was examined using the colony formation assay after X-ray irradiation of rat ileal epithelial cells (IEC-18). The addition of 1μM NE decreased the surviving fraction of cells irradiated with 6Gy from 37% to 8%. The radiosensitivity of IEC-18 cells was enhanced by the addition of 1μM of NE. The irradiation and treatment with NE also resulted in an increased cellular apoptotic rate. These results showing enhanced radiosensitivity of rat ileal epithelial cells by NE suggest that NE may be one of the factors which aggravate acute radiation injury in the intestine. [INTRODUCTION] Spontaneously hypertensive rats, originally derived from Wistar Kyoto rats, are a well-known model for studying essential hypertension in humans.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - radiation effects</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Hypertension</subject><subject>Ileum - drug effects</subject><subject>Ileum - physiology</subject><subject>Ileum - radiation effects</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - physiology</subject><subject>Intestinal Mucosa - radiation effects</subject><subject>Norepinephrine</subject><subject>Norepinephrine - pharmacology</subject><subject>Radiation (Physics)</subject><subject>Radiation Dosage</subject><subject>Radiation Tolerance - drug effects</subject><subject>Radiation Tolerance - physiology</subject><subject>Radiation-Sensitizing Agents - pharmacology</subject><subject>Rats</subject><subject>Rats, Inbred WKY</subject><issn>0449-3060</issn><issn>1349-9157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kVFrFDEUhYNY7FL74B-QAQXxYbe5ySSTeSzLthaKhaLPIZPJuFmzyZjMCP333nWrRREJJJfLdw8n9xDyCugKmGwvdjmvgIIQz8gCeN0uWxDNc7KgNdacSnpKzkvxHSKSUgXwgpwyBLls-IJcfUzZjT66cZvxrjZxa6J1pbo3vU_FxeIn_91PD5WP2Juqm-BMqDajn7YueCzXLoTykpwMJhR3_viekc9Xm0_rD8vbu-ub9eXt0kqhpmXNh77tzGBE0zGjBi4GS4ELxhwXFCSvu4GBEn3fWCFbBR0g1XLbO8qYrPkZeXfUHXP6Nrsy6b0vFh2Y6NJctJJt0yrVHMg3f5G7NOeI5jTUuBpBW8aeqC8mOO3jkKZs7EFTXwouQdRSAVKrf1B4erf3NkU3eOz_MfD-OGBzKiW7QY_Z701-0ED1ITWNqemfqSH7-tHo3O1d_5v8lRECb49Amsf_6lwfMRTx1oQUAwb69Gv7Ve5Szk4zCqApFYxyjTvXlKMO5Q2HWjYKQ_gB516xJg</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Matsuu-Matsuyama, Mutsumi</creator><creator>Okaichi, Kumio</creator><creator>Shichijo, Kazuko</creator><creator>Nakayama, Toshiyuki</creator><creator>Nakashima, Masahiro</creator><creator>Sekine, Ichiro</creator><general>THE JAPAN RADIATION RESEARCH SOCIETY</general><general>The Japan Radiation Research Society and Japanese Society for Therapeutic Radiology and Oncology</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>2011</creationdate><title>Norepinephrine Enhances Radiosensitivity in Rat Ileal Epithelial Cells</title><author>Matsuu-Matsuyama, Mutsumi ; Okaichi, Kumio ; Shichijo, Kazuko ; Nakayama, Toshiyuki ; Nakashima, Masahiro ; Sekine, Ichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c658t-43fd9bafa57b2a8f35fc013522e3501634bf2185dd7c56981b1a8f93cde022643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - radiation effects</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Radiation</topic><topic>Hypertension</topic><topic>Ileum - drug effects</topic><topic>Ileum - physiology</topic><topic>Ileum - radiation effects</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - physiology</topic><topic>Intestinal Mucosa - radiation effects</topic><topic>Norepinephrine</topic><topic>Norepinephrine - pharmacology</topic><topic>Radiation (Physics)</topic><topic>Radiation Dosage</topic><topic>Radiation Tolerance - drug effects</topic><topic>Radiation Tolerance - physiology</topic><topic>Radiation-Sensitizing Agents - pharmacology</topic><topic>Rats</topic><topic>Rats, Inbred WKY</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsuu-Matsuyama, Mutsumi</creatorcontrib><creatorcontrib>Okaichi, Kumio</creatorcontrib><creatorcontrib>Shichijo, Kazuko</creatorcontrib><creatorcontrib>Nakayama, Toshiyuki</creatorcontrib><creatorcontrib>Nakashima, Masahiro</creatorcontrib><creatorcontrib>Sekine, Ichiro</creatorcontrib><creatorcontrib>Department of Tumor and Diagnostic Pathology</creatorcontrib><creatorcontrib>Department of Pathology</creatorcontrib><creatorcontrib>Tissue and Histopathology Section</creatorcontrib><creatorcontrib>Nagasaki Red Cross Blood Center</creatorcontrib><creatorcontrib>Department of Radiation Biophysics</creatorcontrib><creatorcontrib>Nagasaki University Graduate School of Biomedical Sciences</creatorcontrib><creatorcontrib>Atomic Bomb Disease Institute</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>JOURNAL OF RADIATION RESEARCH</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsuu-Matsuyama, Mutsumi</au><au>Okaichi, Kumio</au><au>Shichijo, Kazuko</au><au>Nakayama, Toshiyuki</au><au>Nakashima, Masahiro</au><au>Sekine, Ichiro</au><aucorp>Department of Tumor and Diagnostic Pathology</aucorp><aucorp>Department of Pathology</aucorp><aucorp>Tissue and Histopathology Section</aucorp><aucorp>Nagasaki Red Cross Blood Center</aucorp><aucorp>Department of Radiation Biophysics</aucorp><aucorp>Nagasaki University Graduate School of Biomedical Sciences</aucorp><aucorp>Atomic Bomb Disease Institute</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Norepinephrine Enhances Radiosensitivity in Rat Ileal Epithelial Cells</atitle><jtitle>JOURNAL OF RADIATION RESEARCH</jtitle><addtitle>J Radiat Res</addtitle><date>2011</date><risdate>2011</risdate><volume>52</volume><issue>3</issue><spage>369</spage><epage>373</epage><pages>369-373</pages><issn>0449-3060</issn><eissn>1349-9157</eissn><abstract>We previously reported that the apoptosis index in jejunal crypt cells after X irradiation was greater in spontaneously hypertensive rats than in Wistar-Kyoto rats. Moreover, these same cells showed a suppression of apoptosis when reserpine was administered to induce sympathetic dysfunction in spontaneously hypertensive rats or Wistar-Kyoto rats1). Whether the hyperfunction of the sympathetic nervous system is involved in the high susceptibility of the jejunal crypt cells to radiation-induced apoptosis was the subject of this study. The effect of norepinephrine (NE) on cell survival was examined using the colony formation assay after X-ray irradiation of rat ileal epithelial cells (IEC-18). The addition of 1μM NE decreased the surviving fraction of cells irradiated with 6Gy from 37% to 8%. The radiosensitivity of IEC-18 cells was enhanced by the addition of 1μM of NE. The irradiation and treatment with NE also resulted in an increased cellular apoptotic rate. These results showing enhanced radiosensitivity of rat ileal epithelial cells by NE suggest that NE may be one of the factors which aggravate acute radiation injury in the intestine. [INTRODUCTION] Spontaneously hypertensive rats, originally derived from Wistar Kyoto rats, are a well-known model for studying essential hypertension in humans.</abstract><cop>England</cop><pub>THE JAPAN RADIATION RESEARCH SOCIETY</pub><pmid>21343673</pmid><doi>10.1269/jrr.10155</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Apoptosis Cell Survival - drug effects Cell Survival - radiation effects Cells, Cultured Dose-Response Relationship, Radiation Hypertension Ileum - drug effects Ileum - physiology Ileum - radiation effects Intestinal Mucosa - drug effects Intestinal Mucosa - physiology Intestinal Mucosa - radiation effects Norepinephrine Norepinephrine - pharmacology Radiation (Physics) Radiation Dosage Radiation Tolerance - drug effects Radiation Tolerance - physiology Radiation-Sensitizing Agents - pharmacology Rats Rats, Inbred WKY |
| title | Norepinephrine Enhances Radiosensitivity in Rat Ileal Epithelial Cells |
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