A Glucose Biosensor Based on Immobilization of Glucose Oxidase in Electropolymerized o-Aminophenol Film on Platinized Glassy Carbon Electrode
A high-performance amperometric glucose biosensor has been developed, based on immobilization of glucose oxidase in an electrochemically synthesized, nonconducting poly(o-aminophenol) film on a platinized glassy carbon electrode. The large microscopic surface area and porous morphology of the platin...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1996-05, Vol.68 (9), p.1632-1638 |
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| container_issue | 9 |
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| container_title | Analytical chemistry (Washington) |
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| creator | Zhang, Zhanen Liu, Haiying Deng, Jiaqi |
| description | A high-performance amperometric glucose biosensor has been developed, based on immobilization of glucose oxidase in an electrochemically synthesized, nonconducting poly(o-aminophenol) film on a platinized glassy carbon electrode. The large microscopic surface area and porous morphology of the platinized glassy carbon electrode result in high enzyme loading, and the enzyme entrapped in the electrodeposited platinum microparticle matrix is stabler than that on a platinum disk electrode surface. The response current of the sensor is 20-fold higher than that of the sensor prepared with a platinum disk electrode of the same geometric area. The experiments showed that the high sensitivity of the sensor is due not only to the large microscopic area but also to the high efficiency of transformation of H2O2 generated by enzymatic reaction to current signal on the platinized glassy carbon electrode. The response time of the sensor is 10 months. |
| doi_str_mv | 10.1021/ac950431d |
| format | Article |
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The large microscopic surface area and porous morphology of the platinized glassy carbon electrode result in high enzyme loading, and the enzyme entrapped in the electrodeposited platinum microparticle matrix is stabler than that on a platinum disk electrode surface. The response current of the sensor is 20-fold higher than that of the sensor prepared with a platinum disk electrode of the same geometric area. The experiments showed that the high sensitivity of the sensor is due not only to the large microscopic area but also to the high efficiency of transformation of H2O2 generated by enzymatic reaction to current signal on the platinized glassy carbon electrode. The response time of the sensor is <4 s, and its lifetime is >10 months.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac950431d</identifier><identifier>PMID: 21619130</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Biological and medical sciences ; Biosensors ; Biotechnology ; Chemistry ; Electrochemistry ; Electrodes: preparations and properties ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General and physical chemistry ; Ion selective electrodes ; Methods. Procedures. 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Chem</addtitle><description>A high-performance amperometric glucose biosensor has been developed, based on immobilization of glucose oxidase in an electrochemically synthesized, nonconducting poly(o-aminophenol) film on a platinized glassy carbon electrode. The large microscopic surface area and porous morphology of the platinized glassy carbon electrode result in high enzyme loading, and the enzyme entrapped in the electrodeposited platinum microparticle matrix is stabler than that on a platinum disk electrode surface. The response current of the sensor is 20-fold higher than that of the sensor prepared with a platinum disk electrode of the same geometric area. The experiments showed that the high sensitivity of the sensor is due not only to the large microscopic area but also to the high efficiency of transformation of H2O2 generated by enzymatic reaction to current signal on the platinized glassy carbon electrode. The response time of the sensor is <4 s, and its lifetime is >10 months.</description><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Electrochemistry</subject><subject>Electrodes: preparations and properties</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General and physical chemistry</subject><subject>Ion selective electrodes</subject><subject>Methods. Procedures. 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Psychology</topic><topic>General and physical chemistry</topic><topic>Ion selective electrodes</topic><topic>Methods. Procedures. Technologies</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Zhanen</creatorcontrib><creatorcontrib>Liu, Haiying</creatorcontrib><creatorcontrib>Deng, Jiaqi</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Zhanen</au><au>Liu, Haiying</au><au>Deng, Jiaqi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Glucose Biosensor Based on Immobilization of Glucose Oxidase in Electropolymerized o-Aminophenol Film on Platinized Glassy Carbon Electrode</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>1996-05-01</date><risdate>1996</risdate><volume>68</volume><issue>9</issue><spage>1632</spage><epage>1638</epage><pages>1632-1638</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A high-performance amperometric glucose biosensor has been developed, based on immobilization of glucose oxidase in an electrochemically synthesized, nonconducting poly(o-aminophenol) film on a platinized glassy carbon electrode. The large microscopic surface area and porous morphology of the platinized glassy carbon electrode result in high enzyme loading, and the enzyme entrapped in the electrodeposited platinum microparticle matrix is stabler than that on a platinum disk electrode surface. The response current of the sensor is 20-fold higher than that of the sensor prepared with a platinum disk electrode of the same geometric area. The experiments showed that the high sensitivity of the sensor is due not only to the large microscopic area but also to the high efficiency of transformation of H2O2 generated by enzymatic reaction to current signal on the platinized glassy carbon electrode. The response time of the sensor is <4 s, and its lifetime is >10 months.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>21619130</pmid><doi>10.1021/ac950431d</doi><tpages>7</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 1996-05, Vol.68 (9), p.1632-1638 |
| issn | 0003-2700 1520-6882 |
| language | eng |
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| source | American Chemical Society Journals |
| subjects | Biological and medical sciences Biosensors Biotechnology Chemistry Electrochemistry Electrodes: preparations and properties Exact sciences and technology Fundamental and applied biological sciences. Psychology General and physical chemistry Ion selective electrodes Methods. Procedures. Technologies Various methods and equipments |
| title | A Glucose Biosensor Based on Immobilization of Glucose Oxidase in Electropolymerized o-Aminophenol Film on Platinized Glassy Carbon Electrode |
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