Periosteal microcirculatory action of chronic estrogen supplementation in osteoporotic rats challenged with tourniquet ischemia
Transient ischemia of osteoporotic bones during elective orthopedic surgery or fracture repair carries risks for serious complications, and estrogen loss or replacement has a potential to influence ischemia–reperfusion-induced inflammatory activation. To clarify this, we investigated the periosteal...
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| Veröffentlicht in: | Life sciences (1973) 2011-01, Vol.88 (3), p.156-162 |
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| creator | Szabó, Andrea Hartmann, Petra Varga, Renata Jánvári, Kristóf Lendvai, Zsanett Szalai, Irén Gomez, Izabella Varga, Gabriella Greksa, Ferenc Németh, István Rázga, Zsolt Keresztes, Margit Garab, Dénes Boros, Mihály |
| description | Transient ischemia of osteoporotic bones during elective orthopedic surgery or fracture repair carries risks for serious complications, and estrogen loss or replacement has a potential to influence ischemia–reperfusion-induced inflammatory activation. To clarify this, we investigated the periosteal inflammatory changes in a clinically relevant time frame in ovariectomized rats, an experimental model of postmenopausal bone loss. Furthermore, the effects of chronic estrogen supplementation on the postischemic local and systemic inflammatory reactions were assessed.
Bilateral ovariectomy or sham operation was performed in 3-month-old female Sprague–Dawley rats. Five months later, estrogen replacement therapy with 17β-estradiol (20
μg
−
1
kg
−
1
day
−
1
) or vehicle treatment was initiated. The microcirculatory inflammatory consequences of 60-min total hindlimb ischemia followed by 180-min reperfusion were examined 11
months after ovariectomy and were compared with those in 3-month-old animals.
The osteoporosis that developed 5
months after ovariectomy was significantly ameliorated by estrogen replacement therapy. Both in ovariectomized and in non-ovariectomized animals, ischemia–reperfusion elevated the neutrophil adherence ~
3-fold in the postcapillary venules of the periosteum (intravital microscopy), with an ~
50–60% increase in intravascular neutrophil activation (CD11b; FACS analysis), an enhanced TNF-α release (ELISA) and periosteal expression of ICAM-1 (the endothelial ligand of CD11b; immunohistochemistry). Exogenous 17β-estradiol considerably reduced TNF-α release and the number of neutrophil–endothelial interactions in the periosteum, without affecting the CD11b and ICAM-1 expression changes.
Osteoporosis itself does not increase the magnitude of the limb ischemia–reperfusion-associated periosteal inflammatory reaction. Chronic estrogen supplementation, however, reverses osteoporosis and significantly ameliorates the microcirculatory consequences of transient ischemia. |
| doi_str_mv | 10.1016/j.lfs.2010.11.004 |
| format | Article |
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Bilateral ovariectomy or sham operation was performed in 3-month-old female Sprague–Dawley rats. Five months later, estrogen replacement therapy with 17β-estradiol (20
μg
−
1
kg
−
1
day
−
1
) or vehicle treatment was initiated. The microcirculatory inflammatory consequences of 60-min total hindlimb ischemia followed by 180-min reperfusion were examined 11
months after ovariectomy and were compared with those in 3-month-old animals.
The osteoporosis that developed 5
months after ovariectomy was significantly ameliorated by estrogen replacement therapy. Both in ovariectomized and in non-ovariectomized animals, ischemia–reperfusion elevated the neutrophil adherence ~
3-fold in the postcapillary venules of the periosteum (intravital microscopy), with an ~
50–60% increase in intravascular neutrophil activation (CD11b; FACS analysis), an enhanced TNF-α release (ELISA) and periosteal expression of ICAM-1 (the endothelial ligand of CD11b; immunohistochemistry). Exogenous 17β-estradiol considerably reduced TNF-α release and the number of neutrophil–endothelial interactions in the periosteum, without affecting the CD11b and ICAM-1 expression changes.
Osteoporosis itself does not increase the magnitude of the limb ischemia–reperfusion-associated periosteal inflammatory reaction. Chronic estrogen supplementation, however, reverses osteoporosis and significantly ameliorates the microcirculatory consequences of transient ischemia.</description><identifier>ISSN: 0024-3205</identifier><identifier>EISSN: 1879-0631</identifier><identifier>DOI: 10.1016/j.lfs.2010.11.004</identifier><identifier>PMID: 21062630</identifier><language>eng</language><publisher>Netherlands: Elsevier Inc</publisher><subject>Analysis of Variance ; Animals ; bone resorption ; CD11b Antigen - metabolism ; Densitometry ; enzyme-linked immunosorbent assay ; estradiol ; Estradiol - pharmacology ; Estrogen Replacement Therapy ; Female ; Flow Cytometry ; Hindlimb - surgery ; Image Processing, Computer-Assisted ; Immunohistochemistry ; Inflammation ; Intravital microscopy ; ischemia ; Ischemia - immunology ; Ischemia - physiopathology ; Ischemia–reperfusion ; long term effects ; Microcirculation - drug effects ; Microcirculation - physiology ; microscopy ; Microscopy, Video ; Neutrophil ; neutrophils ; Neutrophils - immunology ; orthopedics ; osteoporosis ; Osteoporosis - immunology ; Osteoporosis - physiopathology ; Ovariectomy ; periosteum ; Periosteum - blood supply ; postmenopause ; Rats ; Rats, Sprague-Dawley ; risk ; tumor necrosis factor-alpha ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>Life sciences (1973), 2011-01, Vol.88 (3), p.156-162</ispartof><rights>2010</rights><rights>Copyright © 2010. Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-5edbf8f52c5246ad11ba32579d95827922095deaf516db92f3c753614d0a5fee3</citedby><cites>FETCH-LOGICAL-c408t-5edbf8f52c5246ad11ba32579d95827922095deaf516db92f3c753614d0a5fee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.lfs.2010.11.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27911,27912,45982</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21062630$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Szabó, Andrea</creatorcontrib><creatorcontrib>Hartmann, Petra</creatorcontrib><creatorcontrib>Varga, Renata</creatorcontrib><creatorcontrib>Jánvári, Kristóf</creatorcontrib><creatorcontrib>Lendvai, Zsanett</creatorcontrib><creatorcontrib>Szalai, Irén</creatorcontrib><creatorcontrib>Gomez, Izabella</creatorcontrib><creatorcontrib>Varga, Gabriella</creatorcontrib><creatorcontrib>Greksa, Ferenc</creatorcontrib><creatorcontrib>Németh, István</creatorcontrib><creatorcontrib>Rázga, Zsolt</creatorcontrib><creatorcontrib>Keresztes, Margit</creatorcontrib><creatorcontrib>Garab, Dénes</creatorcontrib><creatorcontrib>Boros, Mihály</creatorcontrib><title>Periosteal microcirculatory action of chronic estrogen supplementation in osteoporotic rats challenged with tourniquet ischemia</title><title>Life sciences (1973)</title><addtitle>Life Sci</addtitle><description>Transient ischemia of osteoporotic bones during elective orthopedic surgery or fracture repair carries risks for serious complications, and estrogen loss or replacement has a potential to influence ischemia–reperfusion-induced inflammatory activation. To clarify this, we investigated the periosteal inflammatory changes in a clinically relevant time frame in ovariectomized rats, an experimental model of postmenopausal bone loss. Furthermore, the effects of chronic estrogen supplementation on the postischemic local and systemic inflammatory reactions were assessed.
Bilateral ovariectomy or sham operation was performed in 3-month-old female Sprague–Dawley rats. Five months later, estrogen replacement therapy with 17β-estradiol (20
μg
−
1
kg
−
1
day
−
1
) or vehicle treatment was initiated. The microcirculatory inflammatory consequences of 60-min total hindlimb ischemia followed by 180-min reperfusion were examined 11
months after ovariectomy and were compared with those in 3-month-old animals.
The osteoporosis that developed 5
months after ovariectomy was significantly ameliorated by estrogen replacement therapy. Both in ovariectomized and in non-ovariectomized animals, ischemia–reperfusion elevated the neutrophil adherence ~
3-fold in the postcapillary venules of the periosteum (intravital microscopy), with an ~
50–60% increase in intravascular neutrophil activation (CD11b; FACS analysis), an enhanced TNF-α release (ELISA) and periosteal expression of ICAM-1 (the endothelial ligand of CD11b; immunohistochemistry). Exogenous 17β-estradiol considerably reduced TNF-α release and the number of neutrophil–endothelial interactions in the periosteum, without affecting the CD11b and ICAM-1 expression changes.
Osteoporosis itself does not increase the magnitude of the limb ischemia–reperfusion-associated periosteal inflammatory reaction. Chronic estrogen supplementation, however, reverses osteoporosis and significantly ameliorates the microcirculatory consequences of transient ischemia.</description><subject>Analysis of Variance</subject><subject>Animals</subject><subject>bone resorption</subject><subject>CD11b Antigen - metabolism</subject><subject>Densitometry</subject><subject>enzyme-linked immunosorbent assay</subject><subject>estradiol</subject><subject>Estradiol - pharmacology</subject><subject>Estrogen Replacement Therapy</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Hindlimb - surgery</subject><subject>Image Processing, Computer-Assisted</subject><subject>Immunohistochemistry</subject><subject>Inflammation</subject><subject>Intravital microscopy</subject><subject>ischemia</subject><subject>Ischemia - immunology</subject><subject>Ischemia - physiopathology</subject><subject>Ischemia–reperfusion</subject><subject>long term effects</subject><subject>Microcirculation - drug effects</subject><subject>Microcirculation - physiology</subject><subject>microscopy</subject><subject>Microscopy, Video</subject><subject>Neutrophil</subject><subject>neutrophils</subject><subject>Neutrophils - immunology</subject><subject>orthopedics</subject><subject>osteoporosis</subject><subject>Osteoporosis - immunology</subject><subject>Osteoporosis - physiopathology</subject><subject>Ovariectomy</subject><subject>periosteum</subject><subject>Periosteum - blood supply</subject><subject>postmenopause</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>risk</subject><subject>tumor necrosis factor-alpha</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>0024-3205</issn><issn>1879-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2LFDEQxYMo7rj6B3jRvnnqsSrp9AeeZPELFhR0zyGTVGYydHfaJL2yJ_91M87qUQ9FUfB7j0c9xp4jbBGwfX3cji5tOZxu3AI0D9gG-26ooRX4kG0AeFMLDvKCPUnpCABSduIxu-AILW8FbNjPLxR9SJn0WE3exGB8NOuoc4h3lTbZh7kKrjKHGGZvKko5hj3NVVqXZaSJ5qx_M75gxSUsIYZcwKhzKio9jjTvyVY_fD5UOaxx9t9XypVP5kCT10_ZI6fHRM_u9yW7ef_u29XH-vrzh09Xb69r00Cfa0l253onuZG8abVF3GnBZTfYQfa8GziHQVrSTmJrdwN3wnRStNhY0NIRiUv26uy7xFACpKymEoHGUc8U1qT6thj1ouv-TwqJ2DXIC4lnsrwtpUhOLdFPOt4pBHUqSB1VKUidClKIqhRUNC_u3dfdRPav4k8jBXh5BpwOSu-jT-rma3GQUAbb4US8ORNU_nXrKapkPM2GrI9ksrLB_yPAL3n3rYY</recordid><startdate>20110117</startdate><enddate>20110117</enddate><creator>Szabó, Andrea</creator><creator>Hartmann, Petra</creator><creator>Varga, Renata</creator><creator>Jánvári, Kristóf</creator><creator>Lendvai, Zsanett</creator><creator>Szalai, Irén</creator><creator>Gomez, Izabella</creator><creator>Varga, Gabriella</creator><creator>Greksa, Ferenc</creator><creator>Németh, István</creator><creator>Rázga, Zsolt</creator><creator>Keresztes, Margit</creator><creator>Garab, Dénes</creator><creator>Boros, Mihály</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QP</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20110117</creationdate><title>Periosteal microcirculatory action of chronic estrogen supplementation in osteoporotic rats challenged with tourniquet ischemia</title><author>Szabó, Andrea ; Hartmann, Petra ; Varga, Renata ; Jánvári, Kristóf ; Lendvai, Zsanett ; Szalai, Irén ; Gomez, Izabella ; Varga, Gabriella ; Greksa, Ferenc ; Németh, István ; Rázga, Zsolt ; Keresztes, Margit ; Garab, Dénes ; Boros, Mihály</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-5edbf8f52c5246ad11ba32579d95827922095deaf516db92f3c753614d0a5fee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analysis of Variance</topic><topic>Animals</topic><topic>bone resorption</topic><topic>CD11b Antigen - metabolism</topic><topic>Densitometry</topic><topic>enzyme-linked immunosorbent assay</topic><topic>estradiol</topic><topic>Estradiol - pharmacology</topic><topic>Estrogen Replacement Therapy</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Hindlimb - surgery</topic><topic>Image Processing, Computer-Assisted</topic><topic>Immunohistochemistry</topic><topic>Inflammation</topic><topic>Intravital microscopy</topic><topic>ischemia</topic><topic>Ischemia - immunology</topic><topic>Ischemia - physiopathology</topic><topic>Ischemia–reperfusion</topic><topic>long term effects</topic><topic>Microcirculation - drug effects</topic><topic>Microcirculation - physiology</topic><topic>microscopy</topic><topic>Microscopy, Video</topic><topic>Neutrophil</topic><topic>neutrophils</topic><topic>Neutrophils - immunology</topic><topic>orthopedics</topic><topic>osteoporosis</topic><topic>Osteoporosis - immunology</topic><topic>Osteoporosis - physiopathology</topic><topic>Ovariectomy</topic><topic>periosteum</topic><topic>Periosteum - blood supply</topic><topic>postmenopause</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>risk</topic><topic>tumor necrosis factor-alpha</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Szabó, Andrea</creatorcontrib><creatorcontrib>Hartmann, Petra</creatorcontrib><creatorcontrib>Varga, Renata</creatorcontrib><creatorcontrib>Jánvári, Kristóf</creatorcontrib><creatorcontrib>Lendvai, Zsanett</creatorcontrib><creatorcontrib>Szalai, Irén</creatorcontrib><creatorcontrib>Gomez, Izabella</creatorcontrib><creatorcontrib>Varga, Gabriella</creatorcontrib><creatorcontrib>Greksa, Ferenc</creatorcontrib><creatorcontrib>Németh, István</creatorcontrib><creatorcontrib>Rázga, Zsolt</creatorcontrib><creatorcontrib>Keresztes, Margit</creatorcontrib><creatorcontrib>Garab, Dénes</creatorcontrib><creatorcontrib>Boros, Mihály</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Life sciences (1973)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Szabó, Andrea</au><au>Hartmann, Petra</au><au>Varga, Renata</au><au>Jánvári, Kristóf</au><au>Lendvai, Zsanett</au><au>Szalai, Irén</au><au>Gomez, Izabella</au><au>Varga, Gabriella</au><au>Greksa, Ferenc</au><au>Németh, István</au><au>Rázga, Zsolt</au><au>Keresztes, Margit</au><au>Garab, Dénes</au><au>Boros, Mihály</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Periosteal microcirculatory action of chronic estrogen supplementation in osteoporotic rats challenged with tourniquet ischemia</atitle><jtitle>Life sciences (1973)</jtitle><addtitle>Life Sci</addtitle><date>2011-01-17</date><risdate>2011</risdate><volume>88</volume><issue>3</issue><spage>156</spage><epage>162</epage><pages>156-162</pages><issn>0024-3205</issn><eissn>1879-0631</eissn><abstract>Transient ischemia of osteoporotic bones during elective orthopedic surgery or fracture repair carries risks for serious complications, and estrogen loss or replacement has a potential to influence ischemia–reperfusion-induced inflammatory activation. To clarify this, we investigated the periosteal inflammatory changes in a clinically relevant time frame in ovariectomized rats, an experimental model of postmenopausal bone loss. Furthermore, the effects of chronic estrogen supplementation on the postischemic local and systemic inflammatory reactions were assessed.
Bilateral ovariectomy or sham operation was performed in 3-month-old female Sprague–Dawley rats. Five months later, estrogen replacement therapy with 17β-estradiol (20
μg
−
1
kg
−
1
day
−
1
) or vehicle treatment was initiated. The microcirculatory inflammatory consequences of 60-min total hindlimb ischemia followed by 180-min reperfusion were examined 11
months after ovariectomy and were compared with those in 3-month-old animals.
The osteoporosis that developed 5
months after ovariectomy was significantly ameliorated by estrogen replacement therapy. Both in ovariectomized and in non-ovariectomized animals, ischemia–reperfusion elevated the neutrophil adherence ~
3-fold in the postcapillary venules of the periosteum (intravital microscopy), with an ~
50–60% increase in intravascular neutrophil activation (CD11b; FACS analysis), an enhanced TNF-α release (ELISA) and periosteal expression of ICAM-1 (the endothelial ligand of CD11b; immunohistochemistry). Exogenous 17β-estradiol considerably reduced TNF-α release and the number of neutrophil–endothelial interactions in the periosteum, without affecting the CD11b and ICAM-1 expression changes.
Osteoporosis itself does not increase the magnitude of the limb ischemia–reperfusion-associated periosteal inflammatory reaction. Chronic estrogen supplementation, however, reverses osteoporosis and significantly ameliorates the microcirculatory consequences of transient ischemia.</abstract><cop>Netherlands</cop><pub>Elsevier Inc</pub><pmid>21062630</pmid><doi>10.1016/j.lfs.2010.11.004</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0024-3205 |
| ispartof | Life sciences (1973), 2011-01, Vol.88 (3), p.156-162 |
| issn | 0024-3205 1879-0631 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_869588377 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analysis of Variance Animals bone resorption CD11b Antigen - metabolism Densitometry enzyme-linked immunosorbent assay estradiol Estradiol - pharmacology Estrogen Replacement Therapy Female Flow Cytometry Hindlimb - surgery Image Processing, Computer-Assisted Immunohistochemistry Inflammation Intravital microscopy ischemia Ischemia - immunology Ischemia - physiopathology Ischemia–reperfusion long term effects Microcirculation - drug effects Microcirculation - physiology microscopy Microscopy, Video Neutrophil neutrophils Neutrophils - immunology orthopedics osteoporosis Osteoporosis - immunology Osteoporosis - physiopathology Ovariectomy periosteum Periosteum - blood supply postmenopause Rats Rats, Sprague-Dawley risk tumor necrosis factor-alpha Tumor Necrosis Factor-alpha - metabolism |
| title | Periosteal microcirculatory action of chronic estrogen supplementation in osteoporotic rats challenged with tourniquet ischemia |
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