Human beta defensin-3 engineered keratinocyte sheets constructed by a magnetic force-based tissue engineering technique

A multilayered keratinocyte sheet overexpressing human beta defensin-3 (HBD-3) gene was prepared by the magnetic force-based tissue engineering technique. The cell sheet demonstrated significant antimicrobial activity, indicating that the therapeutic introduction of HBD-3 gene into cell sheets may p...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2009-09, Vol.108 (3), p.244-247
Hauptverfasser:	Ito, Akira, Takahashi, Tetsuya, Kawabe, Yoshinori, Kamihira, Masamichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Antimicrobial Cationic Peptides - chemistry Antimicrobial peptide ANTIMICROBIAL PROPERTIES beta-Defensins - metabolism Biological and medical sciences BIOTECHNOLOGIE BIOTECHNOLOGY BIOTECNOLOGIA Cell Death Cell Line, Tumor Cells, Cultured Dendritic Cells - cytology Fundamental and applied biological sciences. Psychology GENE Gene therapy GENERO HUMANO GENES Genetic Therapy - methods GENRE HUMAIN HBD-3 Humans Keratinocyte Keratinocytes - cytology Magnetics MANKIND Microscopy, Phase-Contrast - methods PEAU PIEL (ANIMAL) PROPIEDADES ANTIMICROBIANAS PROPRIETE ANTIMICROBIENNE Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - metabolism SKIN TERAPIA THERAPEUTIQUE THERAPY Tissue engineering Tissue Engineering - methods
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