Integration of PEGylation and refolding for renaturation of recombinant proteins from insoluble aggregates produced in bacteria—Application to a single-chain Fv fragment

The conjugation of polyethylene glycol (PEGylation) with downsized compact antibodies is an effective method for overcoming the problem of rapid elimination of the compact antibodies from the body. We integrated site-specific PEGylation with the refolding of a single-chain Fv (scFv) of humanized mon...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2010-05, Vol.109 (5), p.447-452
Hauptverfasser:	Kumagai, Izumi, Asano, Ryutaro, Nakanishi, Takeshi, Hashikami, Kentaro, Tanaka, Sho, Badran, Adel, Sanada, Hideaki, Umetsu, Mitsuo
Format:	Artikel
Sprache:	eng
Schlagworte:	ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES ANTIGEN ANTIBODY REACTIONS Biological and medical sciences Biotechnology C-Terminus Conjugation Cysteine Drug Carriers - chemistry Epidermal growth factor receptors ESCHERICHIA COLI Escherichia coli - physiology EXPRESION GENICA EXPRESSION DES GENES FACTEUR DE CROISSANCE FACTORES DE CRECIMIENTO Fundamental and applied biological sciences. Psychology GENE EXPRESSION GROWTH FACTORS http://www.fao.org/aos/agrovoc#c_24051 http://www.fao.org/aos/agrovoc#c_27527 http://www.fao.org/aos/agrovoc#c_33700 http://www.fao.org/aos/agrovoc#c_36557 http://www.fao.org/aos/agrovoc#c_36920 http://www.fao.org/aos/agrovoc#c_503 In vitro refolding Integration MONOCLONAL ANTIBODIES PEGylation Polyethylene glycol Polyethylene Glycols - chemistry Protein Denaturation Protein Engineering - methods Protein Folding PROTEINAS RECOMBINANTES PROTEINE RECOMBINANTE REACCION ANTIGENO ANTICUERPO REACTION ANTIGENE ANTICORPS Recombinant antibody RECOMBINANT PROTEINS Recombinant Proteins - chemistry Recombinant Proteins - metabolism Renaturation scFv Single-Chain Antibodies - chemistry Single-Chain Antibodies - genetics Single-Chain Antibodies - metabolism Solubility Therapeutic protein
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