Droplet-based gene expression analysis using a device with magnetic force-based-droplet-handling system

A droplet-based cell lysis and reverse transcription-polymerase chain reaction (PCR) were performed on-chip employing magnetic force-based-droplet-handling system. The actuation with a magnet offers a simple system for droplet manipulation; it does not need mechanical fluidic systems such as pumps a...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2010-02, Vol.109 (2), p.193-197
Hauptverfasser:	Okochi, Mina, Tsuchiya, Hiroyoshi, Kumazawa, Fumitaka, Shikida, Mitsuhiro, Honda, Hiroyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	ANALISIS HISTOCITOLOGICO ANALYSE HISTOCYTOLOGIQUE Biological and medical sciences Biotechnology Cell Line, Tumor DESARROLLO DE UN PRODUCTO DESEMPENO DE LA MAQUINARIA DEVELOPPEMENT DE PRODUIT Droplet handling EQUIPEMENT MEDICAL EQUIPMENT PERFORMANCE EQUIPO MEDICO Fundamental and applied biological sciences. Psychology Gene Expression Profiling - instrumentation Gene Expression Profiling - methods HeLa Cells HISTOCYTOLOGICAL ANALYSIS http://www.fao.org/aos/agrovoc#c_16018 http://www.fao.org/aos/agrovoc#c_25788 http://www.fao.org/aos/agrovoc#c_27541 http://www.fao.org/aos/agrovoc#c_34079 http://www.fao.org/aos/agrovoc#c_4297 http://www.fao.org/aos/agrovoc#c_4518 http://www.fao.org/aos/agrovoc#c_49886 Humans LEUCEMIA LEUCEMIE LEUKAEMIA Magnetic beads Magnetic manipulation MAGNETIC PROPERTIES Magnetics MEDICAL EQUIPMENT PCR PERFORMANCE DU MATERIEL PRODUCT DEVELOPMENT PROPIEDADES MAGNETICAS PROPRIETE MAGNETIQUE Reverse Transcriptase Polymerase Chain Reaction - methods RT-PCR WT1
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