Droplet-based gene expression analysis using a device with magnetic force-based-droplet-handling system
A droplet-based cell lysis and reverse transcription-polymerase chain reaction (PCR) were performed on-chip employing magnetic force-based-droplet-handling system. The actuation with a magnet offers a simple system for droplet manipulation; it does not need mechanical fluidic systems such as pumps a...
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| Veröffentlicht in: | Journal of bioscience and bioengineering 2010-02, Vol.109 (2), p.193-197 |
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| container_title | Journal of bioscience and bioengineering |
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| creator | Okochi, Mina Tsuchiya, Hiroyoshi Kumazawa, Fumitaka Shikida, Mitsuhiro Honda, Hiroyuki |
| description | A droplet-based cell lysis and reverse transcription-polymerase chain reaction (PCR) were performed on-chip employing magnetic force-based-droplet-handling system. The actuation with a magnet offers a simple system for droplet manipulation; it does not need mechanical fluidic systems such as pumps and valves for handling solutions. It can be used as a powerful tool for various biochemical applications by moving and coalescing sample droplets using magnetic beads immersed in mineral oil. The droplet containing magnetic beads and the cells were manipulated with the magnet located underneath the channel, and coalesced with a droplet of lysis buffer. Using K562 cells as the leukemia model, the cell lysis, cDNA synthesis, and amplification of
WT1 gene that is known as the prognostic factor for acute leukemia were successfully performed from a single cell. |
| doi_str_mv | 10.1016/j.jbiosc.2009.07.005 |
| format | Article |
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WT1 gene that is known as the prognostic factor for acute leukemia were successfully performed from a single cell.</description><subject>ANALISIS HISTOCITOLOGICO</subject><subject>ANALYSE HISTOCYTOLOGIQUE</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell Line, Tumor</subject><subject>DESARROLLO DE UN PRODUCTO</subject><subject>DESEMPENO DE LA MAQUINARIA</subject><subject>DEVELOPPEMENT DE PRODUIT</subject><subject>Droplet handling</subject><subject>EQUIPEMENT MEDICAL</subject><subject>EQUIPMENT PERFORMANCE</subject><subject>EQUIPO MEDICO</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Gene Expression Profiling - instrumentation</topic><topic>Gene Expression Profiling - methods</topic><topic>HeLa Cells</topic><topic>HISTOCYTOLOGICAL ANALYSIS</topic><topic>http://www.fao.org/aos/agrovoc#c_16018</topic><topic>http://www.fao.org/aos/agrovoc#c_25788</topic><topic>http://www.fao.org/aos/agrovoc#c_27541</topic><topic>http://www.fao.org/aos/agrovoc#c_34079</topic><topic>http://www.fao.org/aos/agrovoc#c_4297</topic><topic>http://www.fao.org/aos/agrovoc#c_4518</topic><topic>http://www.fao.org/aos/agrovoc#c_49886</topic><topic>Humans</topic><topic>LEUCEMIA</topic><topic>LEUCEMIE</topic><topic>LEUKAEMIA</topic><topic>Magnetic beads</topic><topic>Magnetic manipulation</topic><topic>MAGNETIC PROPERTIES</topic><topic>Magnetics</topic><topic>MEDICAL EQUIPMENT</topic><topic>PCR</topic><topic>PERFORMANCE DU MATERIEL</topic><topic>PRODUCT DEVELOPMENT</topic><topic>PROPIEDADES MAGNETICAS</topic><topic>PROPRIETE MAGNETIQUE</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RT-PCR</topic><topic>WT1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okochi, Mina</creatorcontrib><creatorcontrib>Tsuchiya, Hiroyoshi</creatorcontrib><creatorcontrib>Kumazawa, Fumitaka</creatorcontrib><creatorcontrib>Shikida, Mitsuhiro</creatorcontrib><creatorcontrib>Honda, Hiroyuki</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of bioscience and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okochi, Mina</au><au>Tsuchiya, Hiroyoshi</au><au>Kumazawa, Fumitaka</au><au>Shikida, Mitsuhiro</au><au>Honda, Hiroyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Droplet-based gene expression analysis using a device with magnetic force-based-droplet-handling system</atitle><jtitle>Journal of bioscience and bioengineering</jtitle><addtitle>J Biosci Bioeng</addtitle><date>2010-02-01</date><risdate>2010</risdate><volume>109</volume><issue>2</issue><spage>193</spage><epage>197</epage><pages>193-197</pages><issn>1389-1723</issn><eissn>1347-4421</eissn><abstract>A droplet-based cell lysis and reverse transcription-polymerase chain reaction (PCR) were performed on-chip employing magnetic force-based-droplet-handling system. The actuation with a magnet offers a simple system for droplet manipulation; it does not need mechanical fluidic systems such as pumps and valves for handling solutions. It can be used as a powerful tool for various biochemical applications by moving and coalescing sample droplets using magnetic beads immersed in mineral oil. The droplet containing magnetic beads and the cells were manipulated with the magnet located underneath the channel, and coalesced with a droplet of lysis buffer. Using K562 cells as the leukemia model, the cell lysis, cDNA synthesis, and amplification of
WT1 gene that is known as the prognostic factor for acute leukemia were successfully performed from a single cell.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20129107</pmid><doi>10.1016/j.jbiosc.2009.07.005</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of bioscience and bioengineering, 2010-02, Vol.109 (2), p.193-197 |
| issn | 1389-1723 1347-4421 |
| language | eng |
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| subjects | ANALISIS HISTOCITOLOGICO ANALYSE HISTOCYTOLOGIQUE Biological and medical sciences Biotechnology Cell Line, Tumor DESARROLLO DE UN PRODUCTO DESEMPENO DE LA MAQUINARIA DEVELOPPEMENT DE PRODUIT Droplet handling EQUIPEMENT MEDICAL EQUIPMENT PERFORMANCE EQUIPO MEDICO Fundamental and applied biological sciences. Psychology Gene Expression Profiling - instrumentation Gene Expression Profiling - methods HeLa Cells HISTOCYTOLOGICAL ANALYSIS http://www.fao.org/aos/agrovoc#c_16018 http://www.fao.org/aos/agrovoc#c_25788 http://www.fao.org/aos/agrovoc#c_27541 http://www.fao.org/aos/agrovoc#c_34079 http://www.fao.org/aos/agrovoc#c_4297 http://www.fao.org/aos/agrovoc#c_4518 http://www.fao.org/aos/agrovoc#c_49886 Humans LEUCEMIA LEUCEMIE LEUKAEMIA Magnetic beads Magnetic manipulation MAGNETIC PROPERTIES Magnetics MEDICAL EQUIPMENT PCR PERFORMANCE DU MATERIEL PRODUCT DEVELOPMENT PROPIEDADES MAGNETICAS PROPRIETE MAGNETIQUE Reverse Transcriptase Polymerase Chain Reaction - methods RT-PCR WT1 |
| title | Droplet-based gene expression analysis using a device with magnetic force-based-droplet-handling system |
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