Heterologous overexpression of quorum-sensing regulators to study cell-density-dependent phenotypes in a symbiotic plant bacterium Mesorhizobium huakuii
Quorum-sensing is widespread among many prokaryotic lineages. In order to investigate quorum regulation in the plant bacterium Mesorhizobium huakuii which produces an N-acyl homoserine lactone (AHL) quorum signal, the Agrobacterium quorum-sensing regulator TraR was heterologously expressed in this b...
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| Veröffentlicht in: | Archives of microbiology 2004-12, Vol.182 (6), p.520-525 |
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| creator | Wang, H Zhong, Z Cai, T Li, S Zhu, J |
| description | Quorum-sensing is widespread among many prokaryotic lineages. In order to investigate quorum regulation in the plant bacterium Mesorhizobium huakuii which produces an N-acyl homoserine lactone (AHL) quorum signal, the Agrobacterium quorum-sensing regulator TraR was heterologously expressed in this bacterium. The resulting strains showed reduced AHL production in the supernatant compared to wild-type, but similar intracellular levels of AHLs were detected, suggesting that M. huakuii AHLs can be bound to intracellular TraR proteins and thus become unavailable for its own quorum systems. M. huakuii overexpressing TraR formed thinner biofilms than the wild-type, suggesting a role played by quorum-sensing in biofilm formation. |
| doi_str_mv | 10.1007/s00203-004-0735-8 |
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In order to investigate quorum regulation in the plant bacterium Mesorhizobium huakuii which produces an N-acyl homoserine lactone (AHL) quorum signal, the Agrobacterium quorum-sensing regulator TraR was heterologously expressed in this bacterium. The resulting strains showed reduced AHL production in the supernatant compared to wild-type, but similar intracellular levels of AHLs were detected, suggesting that M. huakuii AHLs can be bound to intracellular TraR proteins and thus become unavailable for its own quorum systems. M. huakuii overexpressing TraR formed thinner biofilms than the wild-type, suggesting a role played by quorum-sensing in biofilm formation.</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-004-0735-8</identifier><identifier>PMID: 15480575</identifier><identifier>CODEN: AMICCW</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>4-Butyrolactone - analogs & derivatives ; 4-Butyrolactone - biosynthesis ; Agrobacterium ; Alphaproteobacteria - genetics ; Alphaproteobacteria - physiology ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacterial Proteins - physiology ; Bacteriology ; biofilm ; Biofilms ; Biofilms - growth & development ; Biological and medical sciences ; cell density ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; gene overexpression ; genetic transformation ; lactones ; Mesorhizobium huakuii ; Microbiology ; N-acyl-homoserine lactone ; nitrogen-fixing bacteria ; plasmid transfer ; Protein Binding ; quorum sensing ; Rhizobium - genetics ; symbionts ; Symbiosis</subject><ispartof>Archives of microbiology, 2004-12, Vol.182 (6), p.520-525</ispartof><rights>2005 INIST-CNRS</rights><rights>Springer-Verlag 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-c5717a325acee75c2e2dbab64bb53b4ff6883119f88896fda3de57c215d10f6b3</citedby><cites>FETCH-LOGICAL-c412t-c5717a325acee75c2e2dbab64bb53b4ff6883119f88896fda3de57c215d10f6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16389286$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15480575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, H</creatorcontrib><creatorcontrib>Zhong, Z</creatorcontrib><creatorcontrib>Cai, T</creatorcontrib><creatorcontrib>Li, S</creatorcontrib><creatorcontrib>Zhu, J</creatorcontrib><title>Heterologous overexpression of quorum-sensing regulators to study cell-density-dependent phenotypes in a symbiotic plant bacterium Mesorhizobium huakuii</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><description>Quorum-sensing is widespread among many prokaryotic lineages. In order to investigate quorum regulation in the plant bacterium Mesorhizobium huakuii which produces an N-acyl homoserine lactone (AHL) quorum signal, the Agrobacterium quorum-sensing regulator TraR was heterologously expressed in this bacterium. The resulting strains showed reduced AHL production in the supernatant compared to wild-type, but similar intracellular levels of AHLs were detected, suggesting that M. huakuii AHLs can be bound to intracellular TraR proteins and thus become unavailable for its own quorum systems. M. huakuii overexpressing TraR formed thinner biofilms than the wild-type, suggesting a role played by quorum-sensing in biofilm formation.</description><subject>4-Butyrolactone - analogs & derivatives</subject><subject>4-Butyrolactone - biosynthesis</subject><subject>Agrobacterium</subject><subject>Alphaproteobacteria - genetics</subject><subject>Alphaproteobacteria - physiology</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Proteins - physiology</subject><subject>Bacteriology</subject><subject>biofilm</subject><subject>Biofilms</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>cell density</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>gene overexpression</subject><subject>genetic transformation</subject><subject>lactones</subject><subject>Mesorhizobium huakuii</subject><subject>Microbiology</subject><subject>N-acyl-homoserine lactone</subject><subject>nitrogen-fixing bacteria</subject><subject>plasmid transfer</subject><subject>Protein Binding</subject><subject>quorum sensing</subject><subject>Rhizobium - genetics</subject><subject>symbionts</subject><subject>Symbiosis</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc2KFDEUhYMoTk_rA7jRIIyuSvNTqaSWMqgjjLjQAXchlbrVnbGqUpMfsX0SH9cU3TDgwtXlkO_ew8lB6Bklbygh8m0khBFeEVJXRHJRqQdoQ2vOimLfH6IN4YRVquX8DJ3HeEsIZUqpx-iMiloRIcUG_bmCBMGPfudzxP4nBPi1BIjR-Rn7Ad9lH_JURZijm3c4wC6PJvkQcfI4ptwfsIVxrPoVSIcyF5iLSHjZw-zTYYGI3YwNjoepcz45i5fRlPfO2OLs8oQ_Q_Rh7377blX7bH5k556gR4MZIzw9zS26-fD-2-VVdf3l46fLd9eVrSlLlRWSSsOZMBZACsuA9Z3pmrrrBO_qYWiU4pS2QwneNkNveA9CWkZFT8nQdHyLXh_vLsHfZYhJTy6ukcwM5Uu0alohuZSikK_-SzaS1qou8Ba9_Ae89TnMJYVWRIpCsKZA9AjZ4GMMMOgluMmEg6ZEr-3qY7u6tKvXdrUqO89Ph3M3QX-_caqzABcnwERrxiGY2bp4zzVctUyt5i-O3GC8NrtQmJuvjFBOijNhjeR_AUkZusY</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>Wang, H</creator><creator>Zhong, Z</creator><creator>Cai, T</creator><creator>Li, S</creator><creator>Zhu, J</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><scope>7T7</scope></search><sort><creationdate>20041201</creationdate><title>Heterologous overexpression of quorum-sensing regulators to study cell-density-dependent phenotypes in a symbiotic plant bacterium Mesorhizobium huakuii</title><author>Wang, H ; Zhong, Z ; Cai, T ; Li, S ; Zhu, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-c5717a325acee75c2e2dbab64bb53b4ff6883119f88896fda3de57c215d10f6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>4-Butyrolactone - analogs & derivatives</topic><topic>4-Butyrolactone - biosynthesis</topic><topic>Agrobacterium</topic><topic>Alphaproteobacteria - genetics</topic><topic>Alphaproteobacteria - physiology</topic><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Proteins - physiology</topic><topic>Bacteriology</topic><topic>biofilm</topic><topic>Biofilms</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>cell density</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>gene overexpression</topic><topic>genetic transformation</topic><topic>lactones</topic><topic>Mesorhizobium huakuii</topic><topic>Microbiology</topic><topic>N-acyl-homoserine lactone</topic><topic>nitrogen-fixing bacteria</topic><topic>plasmid transfer</topic><topic>Protein Binding</topic><topic>quorum sensing</topic><topic>Rhizobium - genetics</topic><topic>symbionts</topic><topic>Symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, H</creatorcontrib><creatorcontrib>Zhong, Z</creatorcontrib><creatorcontrib>Cai, T</creatorcontrib><creatorcontrib>Li, S</creatorcontrib><creatorcontrib>Zhu, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, H</au><au>Zhong, Z</au><au>Cai, T</au><au>Li, S</au><au>Zhu, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterologous overexpression of quorum-sensing regulators to study cell-density-dependent phenotypes in a symbiotic plant bacterium Mesorhizobium huakuii</atitle><jtitle>Archives of microbiology</jtitle><addtitle>Arch Microbiol</addtitle><date>2004-12-01</date><risdate>2004</risdate><volume>182</volume><issue>6</issue><spage>520</spage><epage>525</epage><pages>520-525</pages><issn>0302-8933</issn><eissn>1432-072X</eissn><coden>AMICCW</coden><abstract>Quorum-sensing is widespread among many prokaryotic lineages. In order to investigate quorum regulation in the plant bacterium Mesorhizobium huakuii which produces an N-acyl homoserine lactone (AHL) quorum signal, the Agrobacterium quorum-sensing regulator TraR was heterologously expressed in this bacterium. The resulting strains showed reduced AHL production in the supernatant compared to wild-type, but similar intracellular levels of AHLs were detected, suggesting that M. huakuii AHLs can be bound to intracellular TraR proteins and thus become unavailable for its own quorum systems. M. huakuii overexpressing TraR formed thinner biofilms than the wild-type, suggesting a role played by quorum-sensing in biofilm formation.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>15480575</pmid><doi>10.1007/s00203-004-0735-8</doi><tpages>6</tpages></addata></record> |
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| subjects | 4-Butyrolactone - analogs & derivatives 4-Butyrolactone - biosynthesis Agrobacterium Alphaproteobacteria - genetics Alphaproteobacteria - physiology Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Proteins - physiology Bacteriology biofilm Biofilms Biofilms - growth & development Biological and medical sciences cell density Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial gene overexpression genetic transformation lactones Mesorhizobium huakuii Microbiology N-acyl-homoserine lactone nitrogen-fixing bacteria plasmid transfer Protein Binding quorum sensing Rhizobium - genetics symbionts Symbiosis |
| title | Heterologous overexpression of quorum-sensing regulators to study cell-density-dependent phenotypes in a symbiotic plant bacterium Mesorhizobium huakuii |
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