Evaluation and application of a molecular method to assess the composition of strongylid nematode populations in sheep with naturally acquired infections
We evaluated the performance of a PCR method for the diagnosis of naturally acquired strongylid nematode infections in sheep ( n = 470; in a temperate climatic zone of south-eastern Australia), using a panel of 100 ‘negative control’ samples from sheep known not to harbour parasitic helminths. We co...
Gespeichert in:
| Veröffentlicht in: | Infection, genetics and evolution genetics and evolution, 2011-07, Vol.11 (5), p.849-854 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 854 |
|---|---|
| container_issue | 5 |
| container_start_page | 849 |
| container_title | Infection, genetics and evolution |
| container_volume | 11 |
| creator | Roeber, Florian Jex, Aaron R. Campbell, Angus J.D. Campbell, Bronwyn E. Anderson, Garry A. Gasser, Robin B. |
| description | We evaluated the performance of a PCR method for the diagnosis of naturally acquired strongylid nematode infections in sheep (
n
=
470; in a temperate climatic zone of south-eastern Australia), using a panel of 100 ‘negative control’ samples from sheep known not to harbour parasitic helminths. We compared the diagnostic sensitivity (98%) and specificity (100%) of this assay against a conventional faecal flotation method and also established a system to rank the contribution of particular strongylid nematodes to the faecal egg counts (FECs) from ‘mixed infections’ in individual sheep. The testing of faecal samples herein revealed that
Teladorsagia circumcincta (80%) and
Trichostrongylus spp. (66%) were most prevalent, followed by
Chabertia ovina (33%),
Oesophagostomum venulosum (28%) and
Haemonchus contortus (1%). For the majority of sheep in this study,
T. circumcincta and
Trichostrongylus spp. represented the largest proportion of strongylid eggs in faecal samples from individual sheep. This is the first large-scale prevalence survey of gastrointestinal nematodes in live sheep using a molecular tool. The ability to rapidly rank strongylid nematodes according to their contribution to mixed infections represents a major advantage over routine coprological methods. This PCR tool has the potential to replace the conventional technique of larval culture. Future efforts will focus on enhancing and adapting this molecular method for high throughput application in routine, diagnostic settings. |
| doi_str_mv | 10.1016/j.meegid.2011.01.013 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_869404730</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1567134811000165</els_id><sourcerecordid>869404730</sourcerecordid><originalsourceid>FETCH-LOGICAL-c415t-db4f0a43a9f8290b2d64e40fdd6bf4ba0317fd2dee6892cb962ba5e451c2cffc3</originalsourceid><addsrcrecordid>eNp90V9rFDEQAPBFFFur30A0L-LTnUk2--9FKKVaoeCD9jnMJpO7HNnNNslW7qP4bc26V_smDCSB32SGmaJ4y-iWUVZ_OmwHxJ3VW04Z29IlymfFOavqZtPwqnl-urNStGfFqxgPlLKG8vZlccYZr-qu6c6L39cP4GZI1o8ERk1gmpxV69sbAmTwDtXsIJAB095rkjyBGDFGkvZIlB8mH-2jjyn4cXd0VpMRB0heI5n8lPMXEYkdSdwjTuSXTXsyQpoDOHckoO5nG1BnYFD9ta-LFwZcxDen86K4-3L98-pmc_v967ery9uNEqxKG90LQ0GU0JmWd7TnuhYoqNG67o3ogZasMZprxLrtuOq7mvdQoaiY4soYVV4UH9d_p-DvZ4xJDjYqdA5G9HOUbd0JKpqSZilWqYKPMaCRU7ADhKNkVC47kQe57kQuO5F0iTKnvTsVmPsB9b-kxyVk8OEEICpwJsCobHxyglcta0V271dnwEvYhWzufuRKFaW5QVrXWXxeBeaBPVgMMiqLo0Kdh6uS1N7-v9c_AXG6hQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>869404730</pqid></control><display><type>article</type><title>Evaluation and application of a molecular method to assess the composition of strongylid nematode populations in sheep with naturally acquired infections</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Roeber, Florian ; Jex, Aaron R. ; Campbell, Angus J.D. ; Campbell, Bronwyn E. ; Anderson, Garry A. ; Gasser, Robin B.</creator><creatorcontrib>Roeber, Florian ; Jex, Aaron R. ; Campbell, Angus J.D. ; Campbell, Bronwyn E. ; Anderson, Garry A. ; Gasser, Robin B.</creatorcontrib><description>We evaluated the performance of a PCR method for the diagnosis of naturally acquired strongylid nematode infections in sheep (
n
=
470; in a temperate climatic zone of south-eastern Australia), using a panel of 100 ‘negative control’ samples from sheep known not to harbour parasitic helminths. We compared the diagnostic sensitivity (98%) and specificity (100%) of this assay against a conventional faecal flotation method and also established a system to rank the contribution of particular strongylid nematodes to the faecal egg counts (FECs) from ‘mixed infections’ in individual sheep. The testing of faecal samples herein revealed that
Teladorsagia circumcincta (80%) and
Trichostrongylus spp. (66%) were most prevalent, followed by
Chabertia ovina (33%),
Oesophagostomum venulosum (28%) and
Haemonchus contortus (1%). For the majority of sheep in this study,
T. circumcincta and
Trichostrongylus spp. represented the largest proportion of strongylid eggs in faecal samples from individual sheep. This is the first large-scale prevalence survey of gastrointestinal nematodes in live sheep using a molecular tool. The ability to rapidly rank strongylid nematodes according to their contribution to mixed infections represents a major advantage over routine coprological methods. This PCR tool has the potential to replace the conventional technique of larval culture. Future efforts will focus on enhancing and adapting this molecular method for high throughput application in routine, diagnostic settings.</description><identifier>ISSN: 1567-1348</identifier><identifier>EISSN: 1567-7257</identifier><identifier>DOI: 10.1016/j.meegid.2011.01.013</identifier><identifier>PMID: 21256979</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Chabertia ; Diagnosis ; diagnostic sensitivity ; DNA, Helminth - genetics ; eggs ; Epidemiology. Vaccinations ; fecal egg count ; feces ; Feces - parasitology ; gastrointestinal nematodes ; General aspects ; Haemonchus contortus ; Infectious diseases ; larvae ; Medical sciences ; mixed infection ; nematode infections ; Oesophagostomum ; Parasites ; Parasitic diseases ; PCR ; Polymerase Chain Reaction ; Sensitivity and Specificity ; Sheep ; sheep diseases ; Sheep Diseases - parasitology ; species diversity ; Strongylid nematodes ; Strongylida - classification ; Strongylida - genetics ; Strongylida Infections - parasitology ; Strongylida Infections - veterinary ; surveys ; Teladorsagia circumcincta ; Trichostrongylus</subject><ispartof>Infection, genetics and evolution, 2011-07, Vol.11 (5), p.849-854</ispartof><rights>2011</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-db4f0a43a9f8290b2d64e40fdd6bf4ba0317fd2dee6892cb962ba5e451c2cffc3</citedby><cites>FETCH-LOGICAL-c415t-db4f0a43a9f8290b2d64e40fdd6bf4ba0317fd2dee6892cb962ba5e451c2cffc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1567134811000165$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24258184$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21256979$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roeber, Florian</creatorcontrib><creatorcontrib>Jex, Aaron R.</creatorcontrib><creatorcontrib>Campbell, Angus J.D.</creatorcontrib><creatorcontrib>Campbell, Bronwyn E.</creatorcontrib><creatorcontrib>Anderson, Garry A.</creatorcontrib><creatorcontrib>Gasser, Robin B.</creatorcontrib><title>Evaluation and application of a molecular method to assess the composition of strongylid nematode populations in sheep with naturally acquired infections</title><title>Infection, genetics and evolution</title><addtitle>Infect Genet Evol</addtitle><description>We evaluated the performance of a PCR method for the diagnosis of naturally acquired strongylid nematode infections in sheep (
n
=
470; in a temperate climatic zone of south-eastern Australia), using a panel of 100 ‘negative control’ samples from sheep known not to harbour parasitic helminths. We compared the diagnostic sensitivity (98%) and specificity (100%) of this assay against a conventional faecal flotation method and also established a system to rank the contribution of particular strongylid nematodes to the faecal egg counts (FECs) from ‘mixed infections’ in individual sheep. The testing of faecal samples herein revealed that
Teladorsagia circumcincta (80%) and
Trichostrongylus spp. (66%) were most prevalent, followed by
Chabertia ovina (33%),
Oesophagostomum venulosum (28%) and
Haemonchus contortus (1%). For the majority of sheep in this study,
T. circumcincta and
Trichostrongylus spp. represented the largest proportion of strongylid eggs in faecal samples from individual sheep. This is the first large-scale prevalence survey of gastrointestinal nematodes in live sheep using a molecular tool. The ability to rapidly rank strongylid nematodes according to their contribution to mixed infections represents a major advantage over routine coprological methods. This PCR tool has the potential to replace the conventional technique of larval culture. Future efforts will focus on enhancing and adapting this molecular method for high throughput application in routine, diagnostic settings.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chabertia</subject><subject>Diagnosis</subject><subject>diagnostic sensitivity</subject><subject>DNA, Helminth - genetics</subject><subject>eggs</subject><subject>Epidemiology. Vaccinations</subject><subject>fecal egg count</subject><subject>feces</subject><subject>Feces - parasitology</subject><subject>gastrointestinal nematodes</subject><subject>General aspects</subject><subject>Haemonchus contortus</subject><subject>Infectious diseases</subject><subject>larvae</subject><subject>Medical sciences</subject><subject>mixed infection</subject><subject>nematode infections</subject><subject>Oesophagostomum</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>PCR</subject><subject>Polymerase Chain Reaction</subject><subject>Sensitivity and Specificity</subject><subject>Sheep</subject><subject>sheep diseases</subject><subject>Sheep Diseases - parasitology</subject><subject>species diversity</subject><subject>Strongylid nematodes</subject><subject>Strongylida - classification</subject><subject>Strongylida - genetics</subject><subject>Strongylida Infections - parasitology</subject><subject>Strongylida Infections - veterinary</subject><subject>surveys</subject><subject>Teladorsagia circumcincta</subject><subject>Trichostrongylus</subject><issn>1567-1348</issn><issn>1567-7257</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90V9rFDEQAPBFFFur30A0L-LTnUk2--9FKKVaoeCD9jnMJpO7HNnNNslW7qP4bc26V_smDCSB32SGmaJ4y-iWUVZ_OmwHxJ3VW04Z29IlymfFOavqZtPwqnl-urNStGfFqxgPlLKG8vZlccYZr-qu6c6L39cP4GZI1o8ERk1gmpxV69sbAmTwDtXsIJAB095rkjyBGDFGkvZIlB8mH-2jjyn4cXd0VpMRB0heI5n8lPMXEYkdSdwjTuSXTXsyQpoDOHckoO5nG1BnYFD9ta-LFwZcxDen86K4-3L98-pmc_v967ery9uNEqxKG90LQ0GU0JmWd7TnuhYoqNG67o3ogZasMZprxLrtuOq7mvdQoaiY4soYVV4UH9d_p-DvZ4xJDjYqdA5G9HOUbd0JKpqSZilWqYKPMaCRU7ADhKNkVC47kQe57kQuO5F0iTKnvTsVmPsB9b-kxyVk8OEEICpwJsCobHxyglcta0V271dnwEvYhWzufuRKFaW5QVrXWXxeBeaBPVgMMiqLo0Kdh6uS1N7-v9c_AXG6hQ</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Roeber, Florian</creator><creator>Jex, Aaron R.</creator><creator>Campbell, Angus J.D.</creator><creator>Campbell, Bronwyn E.</creator><creator>Anderson, Garry A.</creator><creator>Gasser, Robin B.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110701</creationdate><title>Evaluation and application of a molecular method to assess the composition of strongylid nematode populations in sheep with naturally acquired infections</title><author>Roeber, Florian ; Jex, Aaron R. ; Campbell, Angus J.D. ; Campbell, Bronwyn E. ; Anderson, Garry A. ; Gasser, Robin B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-db4f0a43a9f8290b2d64e40fdd6bf4ba0317fd2dee6892cb962ba5e451c2cffc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chabertia</topic><topic>Diagnosis</topic><topic>diagnostic sensitivity</topic><topic>DNA, Helminth - genetics</topic><topic>eggs</topic><topic>Epidemiology. Vaccinations</topic><topic>fecal egg count</topic><topic>feces</topic><topic>Feces - parasitology</topic><topic>gastrointestinal nematodes</topic><topic>General aspects</topic><topic>Haemonchus contortus</topic><topic>Infectious diseases</topic><topic>larvae</topic><topic>Medical sciences</topic><topic>mixed infection</topic><topic>nematode infections</topic><topic>Oesophagostomum</topic><topic>Parasites</topic><topic>Parasitic diseases</topic><topic>PCR</topic><topic>Polymerase Chain Reaction</topic><topic>Sensitivity and Specificity</topic><topic>Sheep</topic><topic>sheep diseases</topic><topic>Sheep Diseases - parasitology</topic><topic>species diversity</topic><topic>Strongylid nematodes</topic><topic>Strongylida - classification</topic><topic>Strongylida - genetics</topic><topic>Strongylida Infections - parasitology</topic><topic>Strongylida Infections - veterinary</topic><topic>surveys</topic><topic>Teladorsagia circumcincta</topic><topic>Trichostrongylus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roeber, Florian</creatorcontrib><creatorcontrib>Jex, Aaron R.</creatorcontrib><creatorcontrib>Campbell, Angus J.D.</creatorcontrib><creatorcontrib>Campbell, Bronwyn E.</creatorcontrib><creatorcontrib>Anderson, Garry A.</creatorcontrib><creatorcontrib>Gasser, Robin B.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Infection, genetics and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roeber, Florian</au><au>Jex, Aaron R.</au><au>Campbell, Angus J.D.</au><au>Campbell, Bronwyn E.</au><au>Anderson, Garry A.</au><au>Gasser, Robin B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation and application of a molecular method to assess the composition of strongylid nematode populations in sheep with naturally acquired infections</atitle><jtitle>Infection, genetics and evolution</jtitle><addtitle>Infect Genet Evol</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>11</volume><issue>5</issue><spage>849</spage><epage>854</epage><pages>849-854</pages><issn>1567-1348</issn><eissn>1567-7257</eissn><abstract>We evaluated the performance of a PCR method for the diagnosis of naturally acquired strongylid nematode infections in sheep (
n
=
470; in a temperate climatic zone of south-eastern Australia), using a panel of 100 ‘negative control’ samples from sheep known not to harbour parasitic helminths. We compared the diagnostic sensitivity (98%) and specificity (100%) of this assay against a conventional faecal flotation method and also established a system to rank the contribution of particular strongylid nematodes to the faecal egg counts (FECs) from ‘mixed infections’ in individual sheep. The testing of faecal samples herein revealed that
Teladorsagia circumcincta (80%) and
Trichostrongylus spp. (66%) were most prevalent, followed by
Chabertia ovina (33%),
Oesophagostomum venulosum (28%) and
Haemonchus contortus (1%). For the majority of sheep in this study,
T. circumcincta and
Trichostrongylus spp. represented the largest proportion of strongylid eggs in faecal samples from individual sheep. This is the first large-scale prevalence survey of gastrointestinal nematodes in live sheep using a molecular tool. The ability to rapidly rank strongylid nematodes according to their contribution to mixed infections represents a major advantage over routine coprological methods. This PCR tool has the potential to replace the conventional technique of larval culture. Future efforts will focus on enhancing and adapting this molecular method for high throughput application in routine, diagnostic settings.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>21256979</pmid><doi>10.1016/j.meegid.2011.01.013</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1567-1348 |
| ispartof | Infection, genetics and evolution, 2011-07, Vol.11 (5), p.849-854 |
| issn | 1567-1348 1567-7257 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_869404730 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Chabertia Diagnosis diagnostic sensitivity DNA, Helminth - genetics eggs Epidemiology. Vaccinations fecal egg count feces Feces - parasitology gastrointestinal nematodes General aspects Haemonchus contortus Infectious diseases larvae Medical sciences mixed infection nematode infections Oesophagostomum Parasites Parasitic diseases PCR Polymerase Chain Reaction Sensitivity and Specificity Sheep sheep diseases Sheep Diseases - parasitology species diversity Strongylid nematodes Strongylida - classification Strongylida - genetics Strongylida Infections - parasitology Strongylida Infections - veterinary surveys Teladorsagia circumcincta Trichostrongylus |
| title | Evaluation and application of a molecular method to assess the composition of strongylid nematode populations in sheep with naturally acquired infections |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T00%3A13%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20and%20application%20of%20a%20molecular%20method%20to%20assess%20the%20composition%20of%20strongylid%20nematode%20populations%20in%20sheep%20with%20naturally%20acquired%20infections&rft.jtitle=Infection,%20genetics%20and%20evolution&rft.au=Roeber,%20Florian&rft.date=2011-07-01&rft.volume=11&rft.issue=5&rft.spage=849&rft.epage=854&rft.pages=849-854&rft.issn=1567-1348&rft.eissn=1567-7257&rft_id=info:doi/10.1016/j.meegid.2011.01.013&rft_dat=%3Cproquest_cross%3E869404730%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=869404730&rft_id=info:pmid/21256979&rft_els_id=S1567134811000165&rfr_iscdi=true |