Syndecan-2 cytoplasmic domain regulates colon cancer cell migration via interaction with syntenin-1
► Syndecan-2 plays a pivotal role in colon cancer cell migration. ► Deletion of the EFYA sequence at the C-terminal of the cytoplasmic domain abolished syndecan-2-mediated cell migration and syndecan-2 interaction with syntenin-1. ► Syntenin-1 expression potentiated colon cancer cell migration induc...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2011-05, Vol.409 (1), p.148-153 |
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| creator | Lee, Hawon Kim, Yeonhee Choi, Youngsil Choi, Sojoong Hong, Eunkyung Oh, Eok-Soo |
| description | ► Syndecan-2 plays a pivotal role in colon cancer cell migration. ► Deletion of the EFYA sequence at the C-terminal of the cytoplasmic domain abolished syndecan-2-mediated cell migration and syndecan-2 interaction with syntenin-1. ► Syntenin-1 expression potentiated colon cancer cell migration induced by syndecan-2, and syndecan-2-mediated cell migration was reduced when syntenin-1 expression was diminished. ► Syndecan-2 cytoplasmic domain regulates colon cancer cell migration via interaction with syntenin-1.
The cell surface heparan sulfate proteoglycan, syndecan-2, is crucial for the tumorigenic activity of colon cancer cells. However, the role played by the cytoplasmic domain of the protein remains unclear. Using colon cancer cells transfected with various syndecan-2-encoding genes with deletions in the cytoplasmic domain, it was shown that syndecan-2-induced migration activity requires the EFYA sequence of the C-terminal region; deletion of these residues abolished the rise in cell migration seen when the wild-type gene was transfected and syndecan-2 interaction with syntenin-1, suggesting that syntenin-1 functioned as a cytosolic signal effector downstream from syndecan-2. Colon cancer cells transfected with the syntenin-1 gene showed increased migratory activity, whereas migration was decreased in cells in which syntenin-1 was knock-down using small inhibitory RNA. In addition, syntenin-1 expression potentiated colon cancer cell migration induced by syndecan-2, and syndecan-2-mediated cell migration was reduced when syntenin-1 expression diminished. However, syntenin-1-mediated migration enhancement was not noted in colon cancer cells transfected with a gene encoding a syndecan-2 mutant lacking the cytoplasmic domain. Furthermore, in line with the increase in cell migration, syntenin-1 mediated Rac activation stimulated by syndecan-2. Together, the data suggest that the cytoplasmic domain of syndecan-2 regulates colon cancer cell migration via interaction with syntenin-1. |
| doi_str_mv | 10.1016/j.bbrc.2011.04.135 |
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The cell surface heparan sulfate proteoglycan, syndecan-2, is crucial for the tumorigenic activity of colon cancer cells. However, the role played by the cytoplasmic domain of the protein remains unclear. Using colon cancer cells transfected with various syndecan-2-encoding genes with deletions in the cytoplasmic domain, it was shown that syndecan-2-induced migration activity requires the EFYA sequence of the C-terminal region; deletion of these residues abolished the rise in cell migration seen when the wild-type gene was transfected and syndecan-2 interaction with syntenin-1, suggesting that syntenin-1 functioned as a cytosolic signal effector downstream from syndecan-2. Colon cancer cells transfected with the syntenin-1 gene showed increased migratory activity, whereas migration was decreased in cells in which syntenin-1 was knock-down using small inhibitory RNA. In addition, syntenin-1 expression potentiated colon cancer cell migration induced by syndecan-2, and syndecan-2-mediated cell migration was reduced when syntenin-1 expression diminished. However, syntenin-1-mediated migration enhancement was not noted in colon cancer cells transfected with a gene encoding a syndecan-2 mutant lacking the cytoplasmic domain. Furthermore, in line with the increase in cell migration, syntenin-1 mediated Rac activation stimulated by syndecan-2. Together, the data suggest that the cytoplasmic domain of syndecan-2 regulates colon cancer cell migration via interaction with syntenin-1.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/j.bbrc.2011.04.135</identifier><identifier>PMID: 21569759</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Cell Line, Tumor ; Cell migration ; Cell Movement ; Colon cancer ; Colonic Neoplasms - metabolism ; Colonic Neoplasms - pathology ; Cytoplasm - metabolism ; Humans ; Protein Structure, Tertiary ; Rac1 ; Sequence Deletion ; Syndecan-2 ; Syndecan-2 - genetics ; Syndecan-2 - metabolism ; Syntenin-1 ; Syntenins - metabolism</subject><ispartof>Biochemical and biophysical research communications, 2011-05, Vol.409 (1), p.148-153</ispartof><rights>2011 Elsevier Inc.</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c355t-56fce4590c0b5f3ace0afa17b01b7a5552b2dc1b276fd21f9c1d447cce8fe71f3</citedby><cites>FETCH-LOGICAL-c355t-56fce4590c0b5f3ace0afa17b01b7a5552b2dc1b276fd21f9c1d447cce8fe71f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0006291X1100742X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21569759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Hawon</creatorcontrib><creatorcontrib>Kim, Yeonhee</creatorcontrib><creatorcontrib>Choi, Youngsil</creatorcontrib><creatorcontrib>Choi, Sojoong</creatorcontrib><creatorcontrib>Hong, Eunkyung</creatorcontrib><creatorcontrib>Oh, Eok-Soo</creatorcontrib><title>Syndecan-2 cytoplasmic domain regulates colon cancer cell migration via interaction with syntenin-1</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>► Syndecan-2 plays a pivotal role in colon cancer cell migration. ► Deletion of the EFYA sequence at the C-terminal of the cytoplasmic domain abolished syndecan-2-mediated cell migration and syndecan-2 interaction with syntenin-1. ► Syntenin-1 expression potentiated colon cancer cell migration induced by syndecan-2, and syndecan-2-mediated cell migration was reduced when syntenin-1 expression was diminished. ► Syndecan-2 cytoplasmic domain regulates colon cancer cell migration via interaction with syntenin-1.
The cell surface heparan sulfate proteoglycan, syndecan-2, is crucial for the tumorigenic activity of colon cancer cells. However, the role played by the cytoplasmic domain of the protein remains unclear. Using colon cancer cells transfected with various syndecan-2-encoding genes with deletions in the cytoplasmic domain, it was shown that syndecan-2-induced migration activity requires the EFYA sequence of the C-terminal region; deletion of these residues abolished the rise in cell migration seen when the wild-type gene was transfected and syndecan-2 interaction with syntenin-1, suggesting that syntenin-1 functioned as a cytosolic signal effector downstream from syndecan-2. Colon cancer cells transfected with the syntenin-1 gene showed increased migratory activity, whereas migration was decreased in cells in which syntenin-1 was knock-down using small inhibitory RNA. In addition, syntenin-1 expression potentiated colon cancer cell migration induced by syndecan-2, and syndecan-2-mediated cell migration was reduced when syntenin-1 expression diminished. However, syntenin-1-mediated migration enhancement was not noted in colon cancer cells transfected with a gene encoding a syndecan-2 mutant lacking the cytoplasmic domain. Furthermore, in line with the increase in cell migration, syntenin-1 mediated Rac activation stimulated by syndecan-2. Together, the data suggest that the cytoplasmic domain of syndecan-2 regulates colon cancer cell migration via interaction with syntenin-1.</description><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement</subject><subject>Colon cancer</subject><subject>Colonic Neoplasms - metabolism</subject><subject>Colonic Neoplasms - pathology</subject><subject>Cytoplasm - metabolism</subject><subject>Humans</subject><subject>Protein Structure, Tertiary</subject><subject>Rac1</subject><subject>Sequence Deletion</subject><subject>Syndecan-2</subject><subject>Syndecan-2 - genetics</subject><subject>Syndecan-2 - metabolism</subject><subject>Syntenin-1</subject><subject>Syntenins - metabolism</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1r3DAQhkVoSDYffyCHoltPdme0lr2GXkrIRyGQQxLITcjjUaLFH1vJm7L_PnI27bGnYYZnXmYeIS4QcgQsv6_zpgmUK0DMochxqQ_EAqGGTCEUX8QCAMpM1fh8LE5iXEMCi7I-EscKdVlXul4IetgNLZMdMiVpN42bzsbek2zH3vpBBn7ZdnbiKGnsxkEmkDhI4q6TvX8JdvJp-uat9MPEwdJH_8dPrzLu0mTwQ4Zn4tDZLvL5Zz0VT9dXj5e32d39za_Ln3cZLbWeMl064kLXQNBot7TEYJ3FqgFsKqu1Vo1qCRtVla5V6GrCtigqIl45rtAtT8W3fe4mjL-3HCfT-zifagcet9GsylU1vw2JVHuSwhhjYGc2wfc27AyCmd2atZndmtmtgcIkt2np62f8tum5_bfyV2YCfuwBTk--eQ4mkuckrPWBaTLt6P-X_w4ihIzb</recordid><startdate>20110527</startdate><enddate>20110527</enddate><creator>Lee, Hawon</creator><creator>Kim, Yeonhee</creator><creator>Choi, Youngsil</creator><creator>Choi, Sojoong</creator><creator>Hong, Eunkyung</creator><creator>Oh, Eok-Soo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110527</creationdate><title>Syndecan-2 cytoplasmic domain regulates colon cancer cell migration via interaction with syntenin-1</title><author>Lee, Hawon ; Kim, Yeonhee ; Choi, Youngsil ; Choi, Sojoong ; Hong, Eunkyung ; Oh, Eok-Soo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c355t-56fce4590c0b5f3ace0afa17b01b7a5552b2dc1b276fd21f9c1d447cce8fe71f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement</topic><topic>Colon cancer</topic><topic>Colonic Neoplasms - metabolism</topic><topic>Colonic Neoplasms - pathology</topic><topic>Cytoplasm - metabolism</topic><topic>Humans</topic><topic>Protein Structure, Tertiary</topic><topic>Rac1</topic><topic>Sequence Deletion</topic><topic>Syndecan-2</topic><topic>Syndecan-2 - genetics</topic><topic>Syndecan-2 - metabolism</topic><topic>Syntenin-1</topic><topic>Syntenins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Hawon</creatorcontrib><creatorcontrib>Kim, Yeonhee</creatorcontrib><creatorcontrib>Choi, Youngsil</creatorcontrib><creatorcontrib>Choi, Sojoong</creatorcontrib><creatorcontrib>Hong, Eunkyung</creatorcontrib><creatorcontrib>Oh, Eok-Soo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Hawon</au><au>Kim, Yeonhee</au><au>Choi, Youngsil</au><au>Choi, Sojoong</au><au>Hong, Eunkyung</au><au>Oh, Eok-Soo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Syndecan-2 cytoplasmic domain regulates colon cancer cell migration via interaction with syntenin-1</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2011-05-27</date><risdate>2011</risdate><volume>409</volume><issue>1</issue><spage>148</spage><epage>153</epage><pages>148-153</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>► Syndecan-2 plays a pivotal role in colon cancer cell migration. ► Deletion of the EFYA sequence at the C-terminal of the cytoplasmic domain abolished syndecan-2-mediated cell migration and syndecan-2 interaction with syntenin-1. ► Syntenin-1 expression potentiated colon cancer cell migration induced by syndecan-2, and syndecan-2-mediated cell migration was reduced when syntenin-1 expression was diminished. ► Syndecan-2 cytoplasmic domain regulates colon cancer cell migration via interaction with syntenin-1.
The cell surface heparan sulfate proteoglycan, syndecan-2, is crucial for the tumorigenic activity of colon cancer cells. However, the role played by the cytoplasmic domain of the protein remains unclear. Using colon cancer cells transfected with various syndecan-2-encoding genes with deletions in the cytoplasmic domain, it was shown that syndecan-2-induced migration activity requires the EFYA sequence of the C-terminal region; deletion of these residues abolished the rise in cell migration seen when the wild-type gene was transfected and syndecan-2 interaction with syntenin-1, suggesting that syntenin-1 functioned as a cytosolic signal effector downstream from syndecan-2. Colon cancer cells transfected with the syntenin-1 gene showed increased migratory activity, whereas migration was decreased in cells in which syntenin-1 was knock-down using small inhibitory RNA. In addition, syntenin-1 expression potentiated colon cancer cell migration induced by syndecan-2, and syndecan-2-mediated cell migration was reduced when syntenin-1 expression diminished. However, syntenin-1-mediated migration enhancement was not noted in colon cancer cells transfected with a gene encoding a syndecan-2 mutant lacking the cytoplasmic domain. Furthermore, in line with the increase in cell migration, syntenin-1 mediated Rac activation stimulated by syndecan-2. Together, the data suggest that the cytoplasmic domain of syndecan-2 regulates colon cancer cell migration via interaction with syntenin-1.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>21569759</pmid><doi>10.1016/j.bbrc.2011.04.135</doi><tpages>6</tpages></addata></record> |
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| subjects | Cell Line, Tumor Cell migration Cell Movement Colon cancer Colonic Neoplasms - metabolism Colonic Neoplasms - pathology Cytoplasm - metabolism Humans Protein Structure, Tertiary Rac1 Sequence Deletion Syndecan-2 Syndecan-2 - genetics Syndecan-2 - metabolism Syntenin-1 Syntenins - metabolism |
| title | Syndecan-2 cytoplasmic domain regulates colon cancer cell migration via interaction with syntenin-1 |
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