Berberine Ameliorates Intestinal Epithelial Tight-Junction Damage and Down-regulates Myosin Light Chain Kinase Pathways in a Mouse Model of Endotoxinemia
Background. This study aimed to examine the protective effect of berberine in endotoxin-induced intestinal tight-junction injury in a mice model of endotoxinemia. Methods. Endotoxinemia was induced by intraperitoneal injection of lipopolysaccharide (10 mg/kg). Mice were randomized to 5 groups: contr...
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| creator | Gu, Lili Li, Ning Gong, Jianfeng Li, Qiurong Zhu, Weiming Li, Jieshou |
| description | Background. This study aimed to examine the protective effect of berberine in endotoxin-induced intestinal tight-junction injury in a mice model of endotoxinemia. Methods. Endotoxinemia was induced by intraperitoneal injection of lipopolysaccharide (10 mg/kg). Mice were randomized to 5 groups: control mice, berberine-treated mice, lipopolysaccharide (LPS)-injected mice, mice pretreated with berberine, and mice administered berberine following LPS injection. Samples were collected 12 h after LPS treatment. Results. Ileal mucosal permeability to fluorescein isothiocyanate dextran assay indicated that berberine reduced the permeability of the gut barrier in endotoxinemia. Transmission electron microscopy revealed that pretreatement with berberine partly prevented ultrastructural disruption of tight junctions by LPS. Immunofluorescence and Western blot analysis were performed, and the results demonstrated that pretreatement with berberine partially reversed the redistribution of tight-junction proteins in colon epithelium and in membrane microdomains. Our data also indicated that pretreatement with berberine could suppress translocation, from cytoplasm to the nucleus, of nuclear factor-kB and myosin light chain kinase activation in the intestinal epithelium. Conclusions. Pretreatement with berberine attenuates disruption of tight junctions in intestinal epithelium in a mice model of endotoxinemia. This may possibly have been mediated through down-regulation of the nuclear factor-kB and myosin light chain kinase pathway. |
| doi_str_mv | 10.1093/infdis/jir147 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_868025887</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>41229922</jstor_id><oup_id>10.1093/infdis/jir147</oup_id><sourcerecordid>41229922</sourcerecordid><originalsourceid>FETCH-LOGICAL-c482t-cf24a1c5267b164a4a02943586bf6ab5f58572a06cde5271c83edd05df6208693</originalsourceid><addsrcrecordid>eNqFkcGO0zAQhi0EYsvCkSPIFwSXsLZjO85x6RZYaAWH5RxNbKd1ldjFTrT0UXhbvKT0ysUejb_5_xkPQi8peU9JXV453xmXrvYuUl49QgsqyqqQkpaP0YIQxgqq6voCPUtpTwjhpayeogtGRc3qmizQ7w82tjY6b_H1YHsXIow24Vufz9F56PHq4MZdfsnhndvuxuLL5PXogsc3MMDWYvAG34R7X0S7nfq_5ZtjSM7j9QOPlzvI8dcsliz-DuPuHo4J5xTgTZhybhOM7XHo8MqbMIZfuZnBwXP0pIM-2Ren-xL9-Li6W34u1t8-3S6v14Xmio2F7hgHqgWTVUslBw6E1bwUSradhFZ0QomKAZHaWMEqqlVpjSHCdJIRJevyEr2ddQ8x_Jzy1M3gkrZ9D97m9holFWFCqSqTxUzqGFKKtmsO0Q0Qjw0lzcMymnkZzbyMzL8-KU_tYM2Z_vf7GXhzAiBp6LsIXufyM8ezb13xzL2buTAd_uv5akb3aQzxDHPKsiNj5R_Ohq3F</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>868025887</pqid></control><display><type>article</type><title>Berberine Ameliorates Intestinal Epithelial Tight-Junction Damage and Down-regulates Myosin Light Chain Kinase Pathways in a Mouse Model of Endotoxinemia</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><source>JSTOR</source><source>Oxford Journals</source><creator>Gu, Lili ; Li, Ning ; Gong, Jianfeng ; Li, Qiurong ; Zhu, Weiming ; Li, Jieshou</creator><creatorcontrib>Gu, Lili ; Li, Ning ; Gong, Jianfeng ; Li, Qiurong ; Zhu, Weiming ; Li, Jieshou</creatorcontrib><description>Background. This study aimed to examine the protective effect of berberine in endotoxin-induced intestinal tight-junction injury in a mice model of endotoxinemia. Methods. Endotoxinemia was induced by intraperitoneal injection of lipopolysaccharide (10 mg/kg). Mice were randomized to 5 groups: control mice, berberine-treated mice, lipopolysaccharide (LPS)-injected mice, mice pretreated with berberine, and mice administered berberine following LPS injection. Samples were collected 12 h after LPS treatment. Results. Ileal mucosal permeability to fluorescein isothiocyanate dextran assay indicated that berberine reduced the permeability of the gut barrier in endotoxinemia. Transmission electron microscopy revealed that pretreatement with berberine partly prevented ultrastructural disruption of tight junctions by LPS. Immunofluorescence and Western blot analysis were performed, and the results demonstrated that pretreatement with berberine partially reversed the redistribution of tight-junction proteins in colon epithelium and in membrane microdomains. Our data also indicated that pretreatement with berberine could suppress translocation, from cytoplasm to the nucleus, of nuclear factor-kB and myosin light chain kinase activation in the intestinal epithelium. Conclusions. Pretreatement with berberine attenuates disruption of tight junctions in intestinal epithelium in a mice model of endotoxinemia. This may possibly have been mediated through down-regulation of the nuclear factor-kB and myosin light chain kinase pathway.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/jir147</identifier><identifier>PMID: 21592990</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; BACTERIA ; Berberine - pharmacology ; Biological and medical sciences ; Colon ; Disease Models, Animal ; Down-Regulation - drug effects ; Endotoxemia - enzymology ; Endotoxemia - metabolism ; Endotoxemia - pathology ; Epithelial cells ; Epithelium ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; Infectious diseases ; Intestinal mucosa ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - enzymology ; Intestinal Mucosa - metabolism ; Intestinal Mucosa - pathology ; Intestines - metabolism ; Intestines - pathology ; Lipopolysaccharides ; Male ; Medical sciences ; Membrane microdomains ; Membrane Proteins - metabolism ; Mice ; Mice, Inbred C57BL ; Microbiology ; Myosin-Light-Chain Kinase - metabolism ; Pretreatment ; Rafts ; Sepsis ; Tight junctions ; Tight Junctions - drug effects ; Tight Junctions - metabolism ; Tight Junctions - pathology ; Transcription Factor RelA - metabolism</subject><ispartof>The Journal of infectious diseases, 2011-06, Vol.203 (11), p.1602-1612</ispartof><rights>Copyright © 2011 Oxford University Press on behalf of the Infectious Diseases Society of America</rights><rights>The Author 2011. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com 2011</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-cf24a1c5267b164a4a02943586bf6ab5f58572a06cde5271c83edd05df6208693</citedby><cites>FETCH-LOGICAL-c482t-cf24a1c5267b164a4a02943586bf6ab5f58572a06cde5271c83edd05df6208693</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/41229922$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/41229922$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,1584,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24258974$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21592990$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gu, Lili</creatorcontrib><creatorcontrib>Li, Ning</creatorcontrib><creatorcontrib>Gong, Jianfeng</creatorcontrib><creatorcontrib>Li, Qiurong</creatorcontrib><creatorcontrib>Zhu, Weiming</creatorcontrib><creatorcontrib>Li, Jieshou</creatorcontrib><title>Berberine Ameliorates Intestinal Epithelial Tight-Junction Damage and Down-regulates Myosin Light Chain Kinase Pathways in a Mouse Model of Endotoxinemia</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Background. This study aimed to examine the protective effect of berberine in endotoxin-induced intestinal tight-junction injury in a mice model of endotoxinemia. Methods. Endotoxinemia was induced by intraperitoneal injection of lipopolysaccharide (10 mg/kg). Mice were randomized to 5 groups: control mice, berberine-treated mice, lipopolysaccharide (LPS)-injected mice, mice pretreated with berberine, and mice administered berberine following LPS injection. Samples were collected 12 h after LPS treatment. Results. Ileal mucosal permeability to fluorescein isothiocyanate dextran assay indicated that berberine reduced the permeability of the gut barrier in endotoxinemia. Transmission electron microscopy revealed that pretreatement with berberine partly prevented ultrastructural disruption of tight junctions by LPS. Immunofluorescence and Western blot analysis were performed, and the results demonstrated that pretreatement with berberine partially reversed the redistribution of tight-junction proteins in colon epithelium and in membrane microdomains. Our data also indicated that pretreatement with berberine could suppress translocation, from cytoplasm to the nucleus, of nuclear factor-kB and myosin light chain kinase activation in the intestinal epithelium. Conclusions. Pretreatement with berberine attenuates disruption of tight junctions in intestinal epithelium in a mice model of endotoxinemia. This may possibly have been mediated through down-regulation of the nuclear factor-kB and myosin light chain kinase pathway.</description><subject>Animals</subject><subject>BACTERIA</subject><subject>Berberine - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Colon</subject><subject>Disease Models, Animal</subject><subject>Down-Regulation - drug effects</subject><subject>Endotoxemia - enzymology</subject><subject>Endotoxemia - metabolism</subject><subject>Endotoxemia - pathology</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Infectious diseases</subject><subject>Intestinal mucosa</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - enzymology</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal Mucosa - pathology</subject><subject>Intestines - metabolism</subject><subject>Intestines - pathology</subject><subject>Lipopolysaccharides</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Membrane microdomains</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microbiology</subject><subject>Myosin-Light-Chain Kinase - metabolism</subject><subject>Pretreatment</subject><subject>Rafts</subject><subject>Sepsis</subject><subject>Tight junctions</subject><subject>Tight Junctions - drug effects</subject><subject>Tight Junctions - metabolism</subject><subject>Tight Junctions - pathology</subject><subject>Transcription Factor RelA - metabolism</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGO0zAQhi0EYsvCkSPIFwSXsLZjO85x6RZYaAWH5RxNbKd1ldjFTrT0UXhbvKT0ysUejb_5_xkPQi8peU9JXV453xmXrvYuUl49QgsqyqqQkpaP0YIQxgqq6voCPUtpTwjhpayeogtGRc3qmizQ7w82tjY6b_H1YHsXIow24Vufz9F56PHq4MZdfsnhndvuxuLL5PXogsc3MMDWYvAG34R7X0S7nfq_5ZtjSM7j9QOPlzvI8dcsliz-DuPuHo4J5xTgTZhybhOM7XHo8MqbMIZfuZnBwXP0pIM-2Ren-xL9-Li6W34u1t8-3S6v14Xmio2F7hgHqgWTVUslBw6E1bwUSradhFZ0QomKAZHaWMEqqlVpjSHCdJIRJevyEr2ddQ8x_Jzy1M3gkrZ9D97m9holFWFCqSqTxUzqGFKKtmsO0Q0Qjw0lzcMymnkZzbyMzL8-KU_tYM2Z_vf7GXhzAiBp6LsIXufyM8ezb13xzL2buTAd_uv5akb3aQzxDHPKsiNj5R_Ohq3F</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>Gu, Lili</creator><creator>Li, Ning</creator><creator>Gong, Jianfeng</creator><creator>Li, Qiurong</creator><creator>Zhu, Weiming</creator><creator>Li, Jieshou</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110601</creationdate><title>Berberine Ameliorates Intestinal Epithelial Tight-Junction Damage and Down-regulates Myosin Light Chain Kinase Pathways in a Mouse Model of Endotoxinemia</title><author>Gu, Lili ; Li, Ning ; Gong, Jianfeng ; Li, Qiurong ; Zhu, Weiming ; Li, Jieshou</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-cf24a1c5267b164a4a02943586bf6ab5f58572a06cde5271c83edd05df6208693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>BACTERIA</topic><topic>Berberine - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Colon</topic><topic>Disease Models, Animal</topic><topic>Down-Regulation - drug effects</topic><topic>Endotoxemia - enzymology</topic><topic>Endotoxemia - metabolism</topic><topic>Endotoxemia - pathology</topic><topic>Epithelial cells</topic><topic>Epithelium</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Infectious diseases</topic><topic>Intestinal mucosa</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - enzymology</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal Mucosa - pathology</topic><topic>Intestines - metabolism</topic><topic>Intestines - pathology</topic><topic>Lipopolysaccharides</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Membrane microdomains</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microbiology</topic><topic>Myosin-Light-Chain Kinase - metabolism</topic><topic>Pretreatment</topic><topic>Rafts</topic><topic>Sepsis</topic><topic>Tight junctions</topic><topic>Tight Junctions - drug effects</topic><topic>Tight Junctions - metabolism</topic><topic>Tight Junctions - pathology</topic><topic>Transcription Factor RelA - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gu, Lili</creatorcontrib><creatorcontrib>Li, Ning</creatorcontrib><creatorcontrib>Gong, Jianfeng</creatorcontrib><creatorcontrib>Li, Qiurong</creatorcontrib><creatorcontrib>Zhu, Weiming</creatorcontrib><creatorcontrib>Li, Jieshou</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gu, Lili</au><au>Li, Ning</au><au>Gong, Jianfeng</au><au>Li, Qiurong</au><au>Zhu, Weiming</au><au>Li, Jieshou</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Berberine Ameliorates Intestinal Epithelial Tight-Junction Damage and Down-regulates Myosin Light Chain Kinase Pathways in a Mouse Model of Endotoxinemia</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>2011-06-01</date><risdate>2011</risdate><volume>203</volume><issue>11</issue><spage>1602</spage><epage>1612</epage><pages>1602-1612</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>Background. This study aimed to examine the protective effect of berberine in endotoxin-induced intestinal tight-junction injury in a mice model of endotoxinemia. Methods. Endotoxinemia was induced by intraperitoneal injection of lipopolysaccharide (10 mg/kg). Mice were randomized to 5 groups: control mice, berberine-treated mice, lipopolysaccharide (LPS)-injected mice, mice pretreated with berberine, and mice administered berberine following LPS injection. Samples were collected 12 h after LPS treatment. Results. Ileal mucosal permeability to fluorescein isothiocyanate dextran assay indicated that berberine reduced the permeability of the gut barrier in endotoxinemia. Transmission electron microscopy revealed that pretreatement with berberine partly prevented ultrastructural disruption of tight junctions by LPS. Immunofluorescence and Western blot analysis were performed, and the results demonstrated that pretreatement with berberine partially reversed the redistribution of tight-junction proteins in colon epithelium and in membrane microdomains. Our data also indicated that pretreatement with berberine could suppress translocation, from cytoplasm to the nucleus, of nuclear factor-kB and myosin light chain kinase activation in the intestinal epithelium. Conclusions. Pretreatement with berberine attenuates disruption of tight junctions in intestinal epithelium in a mice model of endotoxinemia. This may possibly have been mediated through down-regulation of the nuclear factor-kB and myosin light chain kinase pathway.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>21592990</pmid><doi>10.1093/infdis/jir147</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals BACTERIA Berberine - pharmacology Biological and medical sciences Colon Disease Models, Animal Down-Regulation - drug effects Endotoxemia - enzymology Endotoxemia - metabolism Endotoxemia - pathology Epithelial cells Epithelium Fundamental and applied biological sciences. Psychology Immunohistochemistry Infectious diseases Intestinal mucosa Intestinal Mucosa - drug effects Intestinal Mucosa - enzymology Intestinal Mucosa - metabolism Intestinal Mucosa - pathology Intestines - metabolism Intestines - pathology Lipopolysaccharides Male Medical sciences Membrane microdomains Membrane Proteins - metabolism Mice Mice, Inbred C57BL Microbiology Myosin-Light-Chain Kinase - metabolism Pretreatment Rafts Sepsis Tight junctions Tight Junctions - drug effects Tight Junctions - metabolism Tight Junctions - pathology Transcription Factor RelA - metabolism |
| title | Berberine Ameliorates Intestinal Epithelial Tight-Junction Damage and Down-regulates Myosin Light Chain Kinase Pathways in a Mouse Model of Endotoxinemia |
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