Treadmill exercise suppresses muscle cell apoptosis by increasing nerve growth factor levels and stimulating p-phosphatidylinositol 3-kinase activation in the soleus of diabetic rats
We investigated the effects of treadmill exercise performed regularly for 6 weeks on the levels of nerve growth factor (NGF), tyrosine kinase A and p75 receptors, phosphatidylinositol 3-kinase (PI3-K), mitogen-activated protein kinase/extracellular signal-regulated kinase (Erk) 1,2, cyclic AMP respo...
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| Veröffentlicht in: | Journal of physiology and biochemistry 2011-06, Vol.67 (2), p.235-241 |
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| creator | Chae, Chang-Hun Jung, Sung-Lim An, Sang-Hyun Jung, Chan-Kyoung Nam, Sang-Nam Kim, Hyun-Tae |
| description | We investigated the effects of treadmill exercise performed regularly for 6 weeks on the levels of nerve growth factor (NGF), tyrosine kinase A and p75 receptors, phosphatidylinositol 3-kinase (PI3-K), mitogen-activated protein kinase/extracellular signal-regulated kinase (Erk) 1,2, cyclic AMP response element-binding protein (CREB), and caspase-3 in the soleus of rats with streptozotocin (STZ)-induced diabetes. Thirty-two male Sprague–Dawley rats were divided into the following four groups: (1) normal control group (NCG;
n
= 8), (2) normal exercise group (NEG;
n
= 8), (3) diabetes control group (DCG;
n
= 8), and (4) diabetes exercise group (DEG;
n
= 8). Diabetes was induced by intraperitoneal injection of STZ (55 mg/kg dissolved in 0.05 M citrate buffer, pH 4.5). Rats were subjected to treadmill exercise 5 days a week for 6 weeks. The protein level of NGF significantly increased in the NEG and DEG (
p
|
| doi_str_mv | 10.1007/s13105-010-0068-9 |
| format | Article |
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n
= 8), (2) normal exercise group (NEG;
n
= 8), (3) diabetes control group (DCG;
n
= 8), and (4) diabetes exercise group (DEG;
n
= 8). Diabetes was induced by intraperitoneal injection of STZ (55 mg/kg dissolved in 0.05 M citrate buffer, pH 4.5). Rats were subjected to treadmill exercise 5 days a week for 6 weeks. The protein level of NGF significantly increased in the NEG and DEG (
p
< 0.001), whereas the levels of tyrosine kinase A and p75 receptors significantly increased in the NEG (
p
< 0.001). The levels of t-PI3-K, p-PI3-K, and p-CREB, and the p-CREB/t-CREB ratio significantly increased in the NEG (
p
< 0.001, respectively). The p-PI3-K/t-PI3-K ratio significantly increased in the DEG (
p
< 0.001). The p-Erk1/t-Erk1 ratio significantly increased in the NEG (
p
< 0.001), whereas the p-Erk2/t-Erk2 ratio significantly decreased in the DCG and DEG (
p
< 0.001). The caspase-3 level significantly increased in the DCG compared with that in the DEG (
p
< 0.001). These results suggest that treadmill exercise increases NGF levels and accelerates p-PI3-K activation in order to suppress apoptotic cell death in the soleus muscle of diabetic rats.]]></description><identifier>ISSN: 1138-7548</identifier><identifier>EISSN: 1877-8755</identifier><identifier>DOI: 10.1007/s13105-010-0068-9</identifier><identifier>PMID: 21207218</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Physiology ; Animals ; Apoptosis ; Biomedical and Life Sciences ; Biomedicine ; Cell Death ; Cyclic AMP Response Element-Binding Protein - metabolism ; Diabetes Mellitus, Experimental - enzymology ; Diabetes Mellitus, Experimental - metabolism ; Exercise Test ; Human Physiology ; Male ; Mitogen-Activated Protein Kinase 3 - metabolism ; Muscle Cells - metabolism ; Muscle, Skeletal - metabolism ; Nerve Growth Factor - metabolism ; Original Paper ; Phosphatidylinositol 3-Kinase - metabolism ; Proto-Oncogene Proteins c-akt - metabolism ; Rats ; Rats, Sprague-Dawley ; Signal Transduction</subject><ispartof>Journal of physiology and biochemistry, 2011-06, Vol.67 (2), p.235-241</ispartof><rights>University of Navarra 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-96e7effe0a27612984e9ea51094133cdc08826d51b997cdb836254749d49ad5f3</citedby><cites>FETCH-LOGICAL-c409t-96e7effe0a27612984e9ea51094133cdc08826d51b997cdb836254749d49ad5f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13105-010-0068-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13105-010-0068-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21207218$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chae, Chang-Hun</creatorcontrib><creatorcontrib>Jung, Sung-Lim</creatorcontrib><creatorcontrib>An, Sang-Hyun</creatorcontrib><creatorcontrib>Jung, Chan-Kyoung</creatorcontrib><creatorcontrib>Nam, Sang-Nam</creatorcontrib><creatorcontrib>Kim, Hyun-Tae</creatorcontrib><title>Treadmill exercise suppresses muscle cell apoptosis by increasing nerve growth factor levels and stimulating p-phosphatidylinositol 3-kinase activation in the soleus of diabetic rats</title><title>Journal of physiology and biochemistry</title><addtitle>J Physiol Biochem</addtitle><addtitle>J Physiol Biochem</addtitle><description><![CDATA[We investigated the effects of treadmill exercise performed regularly for 6 weeks on the levels of nerve growth factor (NGF), tyrosine kinase A and p75 receptors, phosphatidylinositol 3-kinase (PI3-K), mitogen-activated protein kinase/extracellular signal-regulated kinase (Erk) 1,2, cyclic AMP response element-binding protein (CREB), and caspase-3 in the soleus of rats with streptozotocin (STZ)-induced diabetes. Thirty-two male Sprague–Dawley rats were divided into the following four groups: (1) normal control group (NCG;
n
= 8), (2) normal exercise group (NEG;
n
= 8), (3) diabetes control group (DCG;
n
= 8), and (4) diabetes exercise group (DEG;
n
= 8). Diabetes was induced by intraperitoneal injection of STZ (55 mg/kg dissolved in 0.05 M citrate buffer, pH 4.5). Rats were subjected to treadmill exercise 5 days a week for 6 weeks. The protein level of NGF significantly increased in the NEG and DEG (
p
< 0.001), whereas the levels of tyrosine kinase A and p75 receptors significantly increased in the NEG (
p
< 0.001). The levels of t-PI3-K, p-PI3-K, and p-CREB, and the p-CREB/t-CREB ratio significantly increased in the NEG (
p
< 0.001, respectively). The p-PI3-K/t-PI3-K ratio significantly increased in the DEG (
p
< 0.001). The p-Erk1/t-Erk1 ratio significantly increased in the NEG (
p
< 0.001), whereas the p-Erk2/t-Erk2 ratio significantly decreased in the DCG and DEG (
p
< 0.001). The caspase-3 level significantly increased in the DCG compared with that in the DEG (
p
< 0.001). These results suggest that treadmill exercise increases NGF levels and accelerates p-PI3-K activation in order to suppress apoptotic cell death in the soleus muscle of diabetic rats.]]></description><subject>Animal Physiology</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cell Death</subject><subject>Cyclic AMP Response Element-Binding Protein - metabolism</subject><subject>Diabetes Mellitus, Experimental - enzymology</subject><subject>Diabetes Mellitus, Experimental - metabolism</subject><subject>Exercise Test</subject><subject>Human Physiology</subject><subject>Male</subject><subject>Mitogen-Activated Protein Kinase 3 - metabolism</subject><subject>Muscle Cells - metabolism</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Nerve Growth Factor - metabolism</subject><subject>Original Paper</subject><subject>Phosphatidylinositol 3-Kinase - metabolism</subject><subject>Proto-Oncogene Proteins c-akt - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Signal Transduction</subject><issn>1138-7548</issn><issn>1877-8755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcuOFCEUrRiN89APcGPYuWKEKqqApZk4ajKJm3FNaLjVzUgVJZdq7R_z-6TSo0tXF3Ie93Ga5g1nN5wx-R55x1lPGWeUsUFR_ay55EpKqmTfP69v3ikqe6EumivER8ZEy1v2srnYimy5umx-P2SwfgoxEvgF2QUEguuyZEAEJNOKLgJxUHG7pKUkDEh2JxJmV4UY5j2ZIR-B7HP6WQ5ktK6kTCIcISKxsydYwrRGWzbqQpdDwuVQf_4Uw1zdSoqko9_DbGvnKg7HCqa5NiDlUGdJEVYkaSQ-2B2U4Ei2BV81L0YbEV4_1evm293Hh9vP9P7rpy-3H-6pE0wXqgeQMI7AbCsH3molQIPtOdOCd53zjinVDr7nO62l8zvVDW0vpNBeaOv7sbtu3p19l5x-rIDFTAG3a9gZ0opGDYq1Qgy6MvmZ6XJCzDCaJYfJ5pPhzGxpmXNapqZltrTMpnn75L7uJvD_FH_jqYT2TMAKzXvI5jGtea4b_8f1DyQDpP4</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>Chae, Chang-Hun</creator><creator>Jung, Sung-Lim</creator><creator>An, Sang-Hyun</creator><creator>Jung, Chan-Kyoung</creator><creator>Nam, Sang-Nam</creator><creator>Kim, Hyun-Tae</creator><general>Springer Netherlands</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110601</creationdate><title>Treadmill exercise suppresses muscle cell apoptosis by increasing nerve growth factor levels and stimulating p-phosphatidylinositol 3-kinase activation in the soleus of diabetic rats</title><author>Chae, Chang-Hun ; Jung, Sung-Lim ; An, Sang-Hyun ; Jung, Chan-Kyoung ; Nam, Sang-Nam ; Kim, Hyun-Tae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-96e7effe0a27612984e9ea51094133cdc08826d51b997cdb836254749d49ad5f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animal Physiology</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cell Death</topic><topic>Cyclic AMP Response Element-Binding Protein - metabolism</topic><topic>Diabetes Mellitus, Experimental - enzymology</topic><topic>Diabetes Mellitus, Experimental - metabolism</topic><topic>Exercise Test</topic><topic>Human Physiology</topic><topic>Male</topic><topic>Mitogen-Activated Protein Kinase 3 - metabolism</topic><topic>Muscle Cells - metabolism</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Nerve Growth Factor - metabolism</topic><topic>Original Paper</topic><topic>Phosphatidylinositol 3-Kinase - metabolism</topic><topic>Proto-Oncogene Proteins c-akt - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Signal Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chae, Chang-Hun</creatorcontrib><creatorcontrib>Jung, Sung-Lim</creatorcontrib><creatorcontrib>An, Sang-Hyun</creatorcontrib><creatorcontrib>Jung, Chan-Kyoung</creatorcontrib><creatorcontrib>Nam, Sang-Nam</creatorcontrib><creatorcontrib>Kim, Hyun-Tae</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chae, Chang-Hun</au><au>Jung, Sung-Lim</au><au>An, Sang-Hyun</au><au>Jung, Chan-Kyoung</au><au>Nam, Sang-Nam</au><au>Kim, Hyun-Tae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Treadmill exercise suppresses muscle cell apoptosis by increasing nerve growth factor levels and stimulating p-phosphatidylinositol 3-kinase activation in the soleus of diabetic rats</atitle><jtitle>Journal of physiology and biochemistry</jtitle><stitle>J Physiol Biochem</stitle><addtitle>J Physiol Biochem</addtitle><date>2011-06-01</date><risdate>2011</risdate><volume>67</volume><issue>2</issue><spage>235</spage><epage>241</epage><pages>235-241</pages><issn>1138-7548</issn><eissn>1877-8755</eissn><abstract><![CDATA[We investigated the effects of treadmill exercise performed regularly for 6 weeks on the levels of nerve growth factor (NGF), tyrosine kinase A and p75 receptors, phosphatidylinositol 3-kinase (PI3-K), mitogen-activated protein kinase/extracellular signal-regulated kinase (Erk) 1,2, cyclic AMP response element-binding protein (CREB), and caspase-3 in the soleus of rats with streptozotocin (STZ)-induced diabetes. Thirty-two male Sprague–Dawley rats were divided into the following four groups: (1) normal control group (NCG;
n
= 8), (2) normal exercise group (NEG;
n
= 8), (3) diabetes control group (DCG;
n
= 8), and (4) diabetes exercise group (DEG;
n
= 8). Diabetes was induced by intraperitoneal injection of STZ (55 mg/kg dissolved in 0.05 M citrate buffer, pH 4.5). Rats were subjected to treadmill exercise 5 days a week for 6 weeks. The protein level of NGF significantly increased in the NEG and DEG (
p
< 0.001), whereas the levels of tyrosine kinase A and p75 receptors significantly increased in the NEG (
p
< 0.001). The levels of t-PI3-K, p-PI3-K, and p-CREB, and the p-CREB/t-CREB ratio significantly increased in the NEG (
p
< 0.001, respectively). The p-PI3-K/t-PI3-K ratio significantly increased in the DEG (
p
< 0.001). The p-Erk1/t-Erk1 ratio significantly increased in the NEG (
p
< 0.001), whereas the p-Erk2/t-Erk2 ratio significantly decreased in the DCG and DEG (
p
< 0.001). The caspase-3 level significantly increased in the DCG compared with that in the DEG (
p
< 0.001). These results suggest that treadmill exercise increases NGF levels and accelerates p-PI3-K activation in order to suppress apoptotic cell death in the soleus muscle of diabetic rats.]]></abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>21207218</pmid><doi>10.1007/s13105-010-0068-9</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animal Physiology Animals Apoptosis Biomedical and Life Sciences Biomedicine Cell Death Cyclic AMP Response Element-Binding Protein - metabolism Diabetes Mellitus, Experimental - enzymology Diabetes Mellitus, Experimental - metabolism Exercise Test Human Physiology Male Mitogen-Activated Protein Kinase 3 - metabolism Muscle Cells - metabolism Muscle, Skeletal - metabolism Nerve Growth Factor - metabolism Original Paper Phosphatidylinositol 3-Kinase - metabolism Proto-Oncogene Proteins c-akt - metabolism Rats Rats, Sprague-Dawley Signal Transduction |
| title | Treadmill exercise suppresses muscle cell apoptosis by increasing nerve growth factor levels and stimulating p-phosphatidylinositol 3-kinase activation in the soleus of diabetic rats |
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