Fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates
Two step, fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates. We have developed a fluorescence technique for the detection of Hg2+ and Pb2+ ions using polythymine (T33)/benzothiazolium-4-quinolinium dimer derivative (TOTO-3) and polyguanine (G33)/terbium ions (...
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| Veröffentlicht in: | Talanta (Oxford) 2011-04, Vol.84 (2), p.324-329 |
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| creator | Lin, Yang-Wei Liu, Chi-Wei Chang, Huan-Tsung |
| description | Two step, fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates.
We have developed a fluorescence technique for the detection of Hg2+ and Pb2+ ions using polythymine (T33)/benzothiazolium-4-quinolinium dimer derivative (TOTO-3) and polyguanine (G33)/terbium ions (Tb3+) conjugates, respectively. Hg2+ ions induce T33 to form folded structures, leading to increased fluorescence of the T33/TOTO-3 conjugates. Because Pb2+ ions compete with Tb3+ ions to form complexes with G33, the extent of formation of the G33–Tb3+ complexes decreases upon increasing the Pb2+ concentration, leading to decreased fluorescence at 545nm when excited at 290nm. To minimize interference from Hg2+ ions during the detection of Pb2+ ions, we conducted two-step fluorescence measurements; prior to addition of the G33/Tb3+ probe, we recorded the fluorescence of a mixture of the T33/TOTO-3 conjugates and Hg2+ ions. The fluorescence signal obtained was linear with respect to the Hg2+ concentration over the range 25.0–500nM (R2=0.99); for Pb2+ ions, it was linear over the range 3.0–50nM (R2=0.98). The limits of detection (at a signal-to-noise ratio of 3) for Hg2+ and Pb2+ ions were 10.0 and 1.0nM, respectively. Relative to other techniques for the detection of Hg2+ and Pb2+ ions in soil and water samples, our present approach is simpler, faster, and more cost-effective. |
| doi_str_mv | 10.1016/j.talanta.2011.01.016 |
| format | Article |
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We have developed a fluorescence technique for the detection of Hg2+ and Pb2+ ions using polythymine (T33)/benzothiazolium-4-quinolinium dimer derivative (TOTO-3) and polyguanine (G33)/terbium ions (Tb3+) conjugates, respectively. Hg2+ ions induce T33 to form folded structures, leading to increased fluorescence of the T33/TOTO-3 conjugates. Because Pb2+ ions compete with Tb3+ ions to form complexes with G33, the extent of formation of the G33–Tb3+ complexes decreases upon increasing the Pb2+ concentration, leading to decreased fluorescence at 545nm when excited at 290nm. To minimize interference from Hg2+ ions during the detection of Pb2+ ions, we conducted two-step fluorescence measurements; prior to addition of the G33/Tb3+ probe, we recorded the fluorescence of a mixture of the T33/TOTO-3 conjugates and Hg2+ ions. The fluorescence signal obtained was linear with respect to the Hg2+ concentration over the range 25.0–500nM (R2=0.99); for Pb2+ ions, it was linear over the range 3.0–50nM (R2=0.98). The limits of detection (at a signal-to-noise ratio of 3) for Hg2+ and Pb2+ ions were 10.0 and 1.0nM, respectively. Relative to other techniques for the detection of Hg2+ and Pb2+ ions in soil and water samples, our present approach is simpler, faster, and more cost-effective.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2011.01.016</identifier><identifier>PMID: 21376952</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical chemistry ; Aptamer ; Chemistry ; Conjugates ; Derivatives ; Dimers ; Environmental Monitoring - methods ; Exact sciences and technology ; Excitation ; Fluorescence ; Interference ; Lead - analysis ; Lead(II) ; Mercury - analysis ; Mercury(II) ; Polyguanine ; Polythymine ; Signal to noise ratio ; Spectrometric and optical methods ; Spectrometry, Fluorescence - methods ; Terbium ; Water Pollutants, Chemical - analysis</subject><ispartof>Talanta (Oxford), 2011-04, Vol.84 (2), p.324-329</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c572t-b928f7344b1957300ac445a1fce3772cb2123e358e9c1dbceaa33bc5596734433</citedby><cites>FETCH-LOGICAL-c572t-b928f7344b1957300ac445a1fce3772cb2123e358e9c1dbceaa33bc5596734433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2011.01.016$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23952926$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21376952$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Yang-Wei</creatorcontrib><creatorcontrib>Liu, Chi-Wei</creatorcontrib><creatorcontrib>Chang, Huan-Tsung</creatorcontrib><title>Fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>Two step, fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates.
We have developed a fluorescence technique for the detection of Hg2+ and Pb2+ ions using polythymine (T33)/benzothiazolium-4-quinolinium dimer derivative (TOTO-3) and polyguanine (G33)/terbium ions (Tb3+) conjugates, respectively. Hg2+ ions induce T33 to form folded structures, leading to increased fluorescence of the T33/TOTO-3 conjugates. Because Pb2+ ions compete with Tb3+ ions to form complexes with G33, the extent of formation of the G33–Tb3+ complexes decreases upon increasing the Pb2+ concentration, leading to decreased fluorescence at 545nm when excited at 290nm. To minimize interference from Hg2+ ions during the detection of Pb2+ ions, we conducted two-step fluorescence measurements; prior to addition of the G33/Tb3+ probe, we recorded the fluorescence of a mixture of the T33/TOTO-3 conjugates and Hg2+ ions. The fluorescence signal obtained was linear with respect to the Hg2+ concentration over the range 25.0–500nM (R2=0.99); for Pb2+ ions, it was linear over the range 3.0–50nM (R2=0.98). The limits of detection (at a signal-to-noise ratio of 3) for Hg2+ and Pb2+ ions were 10.0 and 1.0nM, respectively. Relative to other techniques for the detection of Hg2+ and Pb2+ ions in soil and water samples, our present approach is simpler, faster, and more cost-effective.</description><subject>Analytical chemistry</subject><subject>Aptamer</subject><subject>Chemistry</subject><subject>Conjugates</subject><subject>Derivatives</subject><subject>Dimers</subject><subject>Environmental Monitoring - methods</subject><subject>Exact sciences and technology</subject><subject>Excitation</subject><subject>Fluorescence</subject><subject>Interference</subject><subject>Lead - analysis</subject><subject>Lead(II)</subject><subject>Mercury - analysis</subject><subject>Mercury(II)</subject><subject>Polyguanine</subject><subject>Polythymine</subject><subject>Signal to noise ratio</subject><subject>Spectrometric and optical methods</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Terbium</subject><subject>Water Pollutants, Chemical - analysis</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EotvCTwDlgiiHbD12HK9PCFUtrFSJCxw4WRNnUnmVjRfbqdR_j8MucKPSSJbl770Zz2PsDfA1cGivduuMI04Z14IDrPlS7TO2go2WtVRaPmcrzqWpDTT8jJ2ntOOcC8nlS3YmQOrWKLFiP27HOURKjiZHVU-ZXPZhqsJQ7Sm6OT5ebrcfKpz6aiTsf1_Ke6rm5Kf7Cg8ZC3cV6RBipli5MO3me8yUXrEXA46JXp_OC_b99ubb9Zf67uvn7fWnu9opLXLdGbEZtGyaDkyZmnN0TaMQBkdSa-E6AUKSVBsyDvrOEaKUnVPKtItKygv2_uh7iOHnTCnbvS_fGctyKMzJblqtG2UUf5osplwp0IW8_C8JuhUArTQLqo6oiyGlSIM9RL_H-GiB2yUpu7OnpOySlOVLtUX39tRi7vbU_1X9iaYA704AJofjEHFyPv3jZIGMWIw-HjkqS37wFG1yfkmz97GEafvgnxjlF7G6spI</recordid><startdate>20110415</startdate><enddate>20110415</enddate><creator>Lin, Yang-Wei</creator><creator>Liu, Chi-Wei</creator><creator>Chang, Huan-Tsung</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QQ</scope><scope>7SR</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><scope>7TV</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20110415</creationdate><title>Fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates</title><author>Lin, Yang-Wei ; Liu, Chi-Wei ; Chang, Huan-Tsung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c572t-b928f7344b1957300ac445a1fce3772cb2123e358e9c1dbceaa33bc5596734433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analytical chemistry</topic><topic>Aptamer</topic><topic>Chemistry</topic><topic>Conjugates</topic><topic>Derivatives</topic><topic>Dimers</topic><topic>Environmental Monitoring - methods</topic><topic>Exact sciences and technology</topic><topic>Excitation</topic><topic>Fluorescence</topic><topic>Interference</topic><topic>Lead - analysis</topic><topic>Lead(II)</topic><topic>Mercury - analysis</topic><topic>Mercury(II)</topic><topic>Polyguanine</topic><topic>Polythymine</topic><topic>Signal to noise ratio</topic><topic>Spectrometric and optical methods</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Terbium</topic><topic>Water Pollutants, Chemical - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Yang-Wei</creatorcontrib><creatorcontrib>Liu, Chi-Wei</creatorcontrib><creatorcontrib>Chang, Huan-Tsung</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Ceramic Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Yang-Wei</au><au>Liu, Chi-Wei</au><au>Chang, Huan-Tsung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2011-04-15</date><risdate>2011</risdate><volume>84</volume><issue>2</issue><spage>324</spage><epage>329</epage><pages>324-329</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>Two step, fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates.
We have developed a fluorescence technique for the detection of Hg2+ and Pb2+ ions using polythymine (T33)/benzothiazolium-4-quinolinium dimer derivative (TOTO-3) and polyguanine (G33)/terbium ions (Tb3+) conjugates, respectively. Hg2+ ions induce T33 to form folded structures, leading to increased fluorescence of the T33/TOTO-3 conjugates. Because Pb2+ ions compete with Tb3+ ions to form complexes with G33, the extent of formation of the G33–Tb3+ complexes decreases upon increasing the Pb2+ concentration, leading to decreased fluorescence at 545nm when excited at 290nm. To minimize interference from Hg2+ ions during the detection of Pb2+ ions, we conducted two-step fluorescence measurements; prior to addition of the G33/Tb3+ probe, we recorded the fluorescence of a mixture of the T33/TOTO-3 conjugates and Hg2+ ions. The fluorescence signal obtained was linear with respect to the Hg2+ concentration over the range 25.0–500nM (R2=0.99); for Pb2+ ions, it was linear over the range 3.0–50nM (R2=0.98). The limits of detection (at a signal-to-noise ratio of 3) for Hg2+ and Pb2+ ions were 10.0 and 1.0nM, respectively. Relative to other techniques for the detection of Hg2+ and Pb2+ ions in soil and water samples, our present approach is simpler, faster, and more cost-effective.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21376952</pmid><doi>10.1016/j.talanta.2011.01.016</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical chemistry Aptamer Chemistry Conjugates Derivatives Dimers Environmental Monitoring - methods Exact sciences and technology Excitation Fluorescence Interference Lead - analysis Lead(II) Mercury - analysis Mercury(II) Polyguanine Polythymine Signal to noise ratio Spectrometric and optical methods Spectrometry, Fluorescence - methods Terbium Water Pollutants, Chemical - analysis |
| title | Fluorescence detection of mercury(II) and lead(II) ions using aptamer/reporter conjugates |
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