Cytotoxic and genotoxic effects of multi-wall carbon nanotubes on human umbilical vein endothelial cells in vitro
Carbon nanomaterials have multiple applications in various areas. However, it has been suggested that exposure to nanoparticles may be a risk for the development of vascular diseases due to injury and dysfunction of the vascular endothelium. Therefore, in the present study, the cytotoxic and genotox...
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| Veröffentlicht in: | Mutation research 2011-04, Vol.721 (2), p.184-191 |
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| creator | Guo, Yuan-Yuan Zhang, Jun Zheng, Yi-Fan Yang, Jun Zhu, Xin-Qiang |
| description | Carbon nanomaterials have multiple applications in various areas. However, it has been suggested that exposure to nanoparticles may be a risk for the development of vascular diseases due to injury and dysfunction of the vascular endothelium. Therefore, in the present study, the cytotoxic and genotoxic effects of multi-wall carbon nanotubes (MWCNTs) on human umbilical vein endothelial cells (HUVECs) were evaluated. Optical and transmission electronic microscopy (TEM) study showed that MWCNTs were able to enter cells rapidly, distribute in the cytoplasm and intracellular vesicles and induce morphological changes. Exposure to MWCNTs reduced the viability of HUVECs, and induced apoptosis in HUVECs. Furthermore, MWCNTs could cause DNA damage as indicated by the formation of γH2AX foci. MWCNTs also affected cellular redox status, e.g., increasing intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels, as well as altering superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) levels. On the other hand, the free radical scavenger N-acetyl-
l-cysteine (NAC) preincubation can inhibit the cytotoxic and genotoxic effects of MWCNTs. Taken together, these results demonstrated that MWCNTs could induce cytotoxic and genotoxic effects in HUVECs, probably through oxidative damage pathways. |
| doi_str_mv | 10.1016/j.mrgentox.2011.01.014 |
| format | Article |
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l-cysteine (NAC) preincubation can inhibit the cytotoxic and genotoxic effects of MWCNTs. Taken together, these results demonstrated that MWCNTs could induce cytotoxic and genotoxic effects in HUVECs, probably through oxidative damage pathways.</description><identifier>ISSN: 1383-5718</identifier><identifier>ISSN: 0027-5107</identifier><identifier>EISSN: 1879-3592</identifier><identifier>DOI: 10.1016/j.mrgentox.2011.01.014</identifier><identifier>PMID: 21296185</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological and medical sciences ; Cell Survival - drug effects ; Cells ; Cells, Cultured ; Cytotoxicity ; DNA Damage ; Dose-Response Relationship, Drug ; Endothelial cells ; Endothelium, Vascular - drug effects ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. Biological and molecular evolution ; Histones - metabolism ; Human exposure ; Humans ; Malondialdehyde ; Medical sciences ; Multi-wall carbon nanotubes (MWCNTs) ; Mutagens - toxicity ; Nanomaterials ; Nanoparticles ; Nanotubes ; Nanotubes, Carbon - toxicity ; Oxidative damage ; Reactive Oxygen Species - metabolism ; Toxicity ; Toxicology ; Transmission electron microscopy ; Umbilical Veins - cytology</subject><ispartof>Mutation research, 2011-04, Vol.721 (2), p.184-191</ispartof><rights>2011 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Apr 3, 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-f7d44aa451cb828dcb6bde6b0813346b1645969d49b520be6c186fe85c6248a53</citedby><cites>FETCH-LOGICAL-c522t-f7d44aa451cb828dcb6bde6b0813346b1645969d49b520be6c186fe85c6248a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1383571811000416$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24084667$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21296185$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Yuan-Yuan</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Zheng, Yi-Fan</creatorcontrib><creatorcontrib>Yang, Jun</creatorcontrib><creatorcontrib>Zhu, Xin-Qiang</creatorcontrib><title>Cytotoxic and genotoxic effects of multi-wall carbon nanotubes on human umbilical vein endothelial cells in vitro</title><title>Mutation research</title><addtitle>Mutat Res</addtitle><description>Carbon nanomaterials have multiple applications in various areas. However, it has been suggested that exposure to nanoparticles may be a risk for the development of vascular diseases due to injury and dysfunction of the vascular endothelium. Therefore, in the present study, the cytotoxic and genotoxic effects of multi-wall carbon nanotubes (MWCNTs) on human umbilical vein endothelial cells (HUVECs) were evaluated. Optical and transmission electronic microscopy (TEM) study showed that MWCNTs were able to enter cells rapidly, distribute in the cytoplasm and intracellular vesicles and induce morphological changes. Exposure to MWCNTs reduced the viability of HUVECs, and induced apoptosis in HUVECs. Furthermore, MWCNTs could cause DNA damage as indicated by the formation of γH2AX foci. MWCNTs also affected cellular redox status, e.g., increasing intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels, as well as altering superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) levels. On the other hand, the free radical scavenger N-acetyl-
l-cysteine (NAC) preincubation can inhibit the cytotoxic and genotoxic effects of MWCNTs. Taken together, these results demonstrated that MWCNTs could induce cytotoxic and genotoxic effects in HUVECs, probably through oxidative damage pathways.</description><subject>Biological and medical sciences</subject><subject>Cell Survival - drug effects</subject><subject>Cells</subject><subject>Cells, Cultured</subject><subject>Cytotoxicity</subject><subject>DNA Damage</subject><subject>Dose-Response Relationship, Drug</subject><subject>Endothelial cells</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Histones - metabolism</subject><subject>Human exposure</subject><subject>Humans</subject><subject>Malondialdehyde</subject><subject>Medical sciences</subject><subject>Multi-wall carbon nanotubes (MWCNTs)</subject><subject>Mutagens - toxicity</subject><subject>Nanomaterials</subject><subject>Nanoparticles</subject><subject>Nanotubes</subject><subject>Nanotubes, Carbon - toxicity</subject><subject>Oxidative damage</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Toxicity</subject><subject>Toxicology</subject><subject>Transmission electron microscopy</subject><subject>Umbilical Veins - cytology</subject><issn>1383-5718</issn><issn>0027-5107</issn><issn>1879-3592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV9rHCEUxaW0NH_arxCkUPo0W3XU0beWpU0Lgbwkz6LOncZlRhOd2TTfvg67aaEvgQvq9XcPh3sQuqBkQwmVn3ebKf-COKffG0Yo3ZC1-Ct0SlWnm1Zo9rreW9U2oqPqBJ2VsiOEkZaot-iEUaYlVeIUPWyf5lRVgsc29rhKHl8wDODngtOAp2WcQ_NoxxF7m12KONqKLQ7qd8R3y2QjXiYXxuDtiPcQIobYp_kOxlAbHsax4Nrchzmnd-jNYMcC74_nObr9_u1m-6O5ur78uf161XjB2NwMXc-5tVxQ7xRTvXfS9SAdUbRtuXRUcqGl7rl2ghEH0lMlB1DCS8aVFe05-nTQvc_pYYEymymU1YqNkJZilOw6zrjWlfzwH7lLS47VXIUYl0oLWSF5gHxOpWQYzH0Ok81PhhKzRmJ25jkSs0ZiyFq8Dl4c1Rc3Qf937DmDCnw8ArbU_Q3ZRh_KP44TxaXsKvflwEHd2j5ANsUHiB76kGtUpk_hJS9_AI4irtM</recordid><startdate>20110403</startdate><enddate>20110403</enddate><creator>Guo, Yuan-Yuan</creator><creator>Zhang, Jun</creator><creator>Zheng, Yi-Fan</creator><creator>Yang, Jun</creator><creator>Zhu, Xin-Qiang</creator><general>Elsevier B.V</general><general>Elsevier</general><general>Elsevier BV</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7TV</scope></search><sort><creationdate>20110403</creationdate><title>Cytotoxic and genotoxic effects of multi-wall carbon nanotubes on human umbilical vein endothelial cells in vitro</title><author>Guo, Yuan-Yuan ; Zhang, Jun ; Zheng, Yi-Fan ; Yang, Jun ; Zhu, Xin-Qiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-f7d44aa451cb828dcb6bde6b0813346b1645969d49b520be6c186fe85c6248a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biological and medical sciences</topic><topic>Cell Survival - drug effects</topic><topic>Cells</topic><topic>Cells, Cultured</topic><topic>Cytotoxicity</topic><topic>DNA Damage</topic><topic>Dose-Response Relationship, Drug</topic><topic>Endothelial cells</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Histones - metabolism</topic><topic>Human exposure</topic><topic>Humans</topic><topic>Malondialdehyde</topic><topic>Medical sciences</topic><topic>Multi-wall carbon nanotubes (MWCNTs)</topic><topic>Mutagens - toxicity</topic><topic>Nanomaterials</topic><topic>Nanoparticles</topic><topic>Nanotubes</topic><topic>Nanotubes, Carbon - toxicity</topic><topic>Oxidative damage</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Toxicity</topic><topic>Toxicology</topic><topic>Transmission electron microscopy</topic><topic>Umbilical Veins - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Yuan-Yuan</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Zheng, Yi-Fan</creatorcontrib><creatorcontrib>Yang, Jun</creatorcontrib><creatorcontrib>Zhu, Xin-Qiang</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><jtitle>Mutation research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Yuan-Yuan</au><au>Zhang, Jun</au><au>Zheng, Yi-Fan</au><au>Yang, Jun</au><au>Zhu, Xin-Qiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytotoxic and genotoxic effects of multi-wall carbon nanotubes on human umbilical vein endothelial cells in vitro</atitle><jtitle>Mutation research</jtitle><addtitle>Mutat Res</addtitle><date>2011-04-03</date><risdate>2011</risdate><volume>721</volume><issue>2</issue><spage>184</spage><epage>191</epage><pages>184-191</pages><issn>1383-5718</issn><issn>0027-5107</issn><eissn>1879-3592</eissn><abstract>Carbon nanomaterials have multiple applications in various areas. However, it has been suggested that exposure to nanoparticles may be a risk for the development of vascular diseases due to injury and dysfunction of the vascular endothelium. Therefore, in the present study, the cytotoxic and genotoxic effects of multi-wall carbon nanotubes (MWCNTs) on human umbilical vein endothelial cells (HUVECs) were evaluated. Optical and transmission electronic microscopy (TEM) study showed that MWCNTs were able to enter cells rapidly, distribute in the cytoplasm and intracellular vesicles and induce morphological changes. Exposure to MWCNTs reduced the viability of HUVECs, and induced apoptosis in HUVECs. Furthermore, MWCNTs could cause DNA damage as indicated by the formation of γH2AX foci. MWCNTs also affected cellular redox status, e.g., increasing intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels, as well as altering superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-Px) levels. On the other hand, the free radical scavenger N-acetyl-
l-cysteine (NAC) preincubation can inhibit the cytotoxic and genotoxic effects of MWCNTs. Taken together, these results demonstrated that MWCNTs could induce cytotoxic and genotoxic effects in HUVECs, probably through oxidative damage pathways.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21296185</pmid><doi>10.1016/j.mrgentox.2011.01.014</doi><tpages>8</tpages></addata></record> |
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| ispartof | Mutation research, 2011-04, Vol.721 (2), p.184-191 |
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| subjects | Biological and medical sciences Cell Survival - drug effects Cells Cells, Cultured Cytotoxicity DNA Damage Dose-Response Relationship, Drug Endothelial cells Endothelium, Vascular - drug effects Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Histones - metabolism Human exposure Humans Malondialdehyde Medical sciences Multi-wall carbon nanotubes (MWCNTs) Mutagens - toxicity Nanomaterials Nanoparticles Nanotubes Nanotubes, Carbon - toxicity Oxidative damage Reactive Oxygen Species - metabolism Toxicity Toxicology Transmission electron microscopy Umbilical Veins - cytology |
| title | Cytotoxic and genotoxic effects of multi-wall carbon nanotubes on human umbilical vein endothelial cells in vitro |
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