Establishment of a novel feline leukemia virus (FeLV)-negative B-cell cell line from a cat with B-cell lymphoma
We established a novel feline B-cell line, MS4, from the neoplastic pleural effusion of a cat with cutaneous B-cell lymphoma. Immunophenotype staining of the MS4 cells was positive for CD20, CD79α, and IgA and negative for CD3, CD4, CD5, CD8α, CD18, CD21, CD22, IgM, IgG, Ig light chain, and MHC clas...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 2011-04, Vol.140 (3-4), p.307-311 |
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| creator | Mochizuki, Hiroyuki Takahashi, Masashi Nishigaki, Kazuo Ide, Tetsuya Goto-Koshino, Yuko Watanabe, Shinya Sato, Hirofumi Sato, Masahiko Kotera, Yukiko Fujino, Yasuhito Ohno, Koichi Uchida, Kazuyuki Tsujimoto, Hajime |
| description | We established a novel feline B-cell line, MS4, from the neoplastic pleural effusion of a cat with cutaneous B-cell lymphoma. Immunophenotype staining of the MS4 cells was positive for CD20, CD79α, and IgA and negative for CD3, CD4, CD5, CD8α, CD18, CD21, CD22, IgM, IgG, Ig light chain, and MHC class II. PCR analysis for immunoglobulin heavy chain gene rearrangements revealed a monoclonal rearrangement, whereas no clonal rearrangement of the T-cell receptor γ gene was detected. Southern blotting with an exogenous feline leukemia virus (FeLV) U3 probe revealed no integration of exogenous FeLV provirus. The MS4 cell line is the first FeLV-negative feline B-cell lymphoma cell line, and may be used to investigate the pathogenesis of spontaneously occurring feline lymphoma and the development of new therapies. |
| doi_str_mv | 10.1016/j.vetimm.2010.12.010 |
| format | Article |
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Immunophenotype staining of the MS4 cells was positive for CD20, CD79α, and IgA and negative for CD3, CD4, CD5, CD8α, CD18, CD21, CD22, IgM, IgG, Ig light chain, and MHC class II. PCR analysis for immunoglobulin heavy chain gene rearrangements revealed a monoclonal rearrangement, whereas no clonal rearrangement of the T-cell receptor γ gene was detected. Southern blotting with an exogenous feline leukemia virus (FeLV) U3 probe revealed no integration of exogenous FeLV provirus. The MS4 cell line is the first FeLV-negative feline B-cell lymphoma cell line, and may be used to investigate the pathogenesis of spontaneously occurring feline lymphoma and the development of new therapies.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/j.vetimm.2010.12.010</identifier><identifier>PMID: 21272942</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; B-cell ; B-Lymphocytes - immunology ; B-Lymphocytes - virology ; Base Sequence ; Cat ; Cat Diseases - genetics ; Cat Diseases - immunology ; Cat Diseases - virology ; Cats ; Cell Line, Tumor ; DNA, Viral - genetics ; DNA, Viral - isolation & purification ; Feline leukemia virus ; FeLV ; Gene Rearrangement, B-Lymphocyte, Heavy Chain ; Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor ; Immunophenotyping ; Leukemia Virus, Feline - genetics ; Leukemia Virus, Feline - isolation & purification ; Lymphoma ; Lymphoma, B-Cell - genetics ; Lymphoma, B-Cell - immunology ; Lymphoma, B-Cell - veterinary ; Lymphoma, B-Cell - virology ; Male</subject><ispartof>Veterinary immunology and immunopathology, 2011-04, Vol.140 (3-4), p.307-311</ispartof><rights>2011 Elsevier B.V.</rights><rights>Copyright © 2011 Elsevier B.V. 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Immunophenotype staining of the MS4 cells was positive for CD20, CD79α, and IgA and negative for CD3, CD4, CD5, CD8α, CD18, CD21, CD22, IgM, IgG, Ig light chain, and MHC class II. PCR analysis for immunoglobulin heavy chain gene rearrangements revealed a monoclonal rearrangement, whereas no clonal rearrangement of the T-cell receptor γ gene was detected. Southern blotting with an exogenous feline leukemia virus (FeLV) U3 probe revealed no integration of exogenous FeLV provirus. The MS4 cell line is the first FeLV-negative feline B-cell lymphoma cell line, and may be used to investigate the pathogenesis of spontaneously occurring feline lymphoma and the development of new therapies.</description><subject>Animals</subject><subject>B-cell</subject><subject>B-Lymphocytes - immunology</subject><subject>B-Lymphocytes - virology</subject><subject>Base Sequence</subject><subject>Cat</subject><subject>Cat Diseases - genetics</subject><subject>Cat Diseases - immunology</subject><subject>Cat Diseases - virology</subject><subject>Cats</subject><subject>Cell Line, Tumor</subject><subject>DNA, Viral - genetics</subject><subject>DNA, Viral - isolation & purification</subject><subject>Feline leukemia virus</subject><subject>FeLV</subject><subject>Gene Rearrangement, B-Lymphocyte, Heavy Chain</subject><subject>Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor</subject><subject>Immunophenotyping</subject><subject>Leukemia Virus, Feline - genetics</subject><subject>Leukemia Virus, Feline - isolation & purification</subject><subject>Lymphoma</subject><subject>Lymphoma, B-Cell - genetics</subject><subject>Lymphoma, B-Cell - immunology</subject><subject>Lymphoma, B-Cell - veterinary</subject><subject>Lymphoma, B-Cell - virology</subject><subject>Male</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EokvhGyDwjXLI4n-J7QsSVC0grcQBytVykknXSxxv7SRVvz1O03KEyzyN9Zs3Iz-EXlOypYRWHw7bGUbn_ZaR5YltszxBG6okL1jJxVO0yVhZMMHkCXqR0oEQUmqlnqMTRplkWrANChdptHXv0t7DMOLQYYuHMEOPO-jdALiH6Td4Z_Hs4pTw2SXsfr0vBri2o5sBfy4a6Ht8X-75LgafPRo74ls37h-B_s4f98Hbl-hZZ_sErx70FF1dXvw8_1rsvn_5dv5pVzSCyrHgtS5Fpbjt2pYpxrXlhFsrG8u0JkJWoKDWijNBKC0lq0vIDaMcWhBKaX6K3q2-xxhuJkij8S4tl9gBwpSMqqQUtGTy_2RZalIJITIpVrKJIaUInTlG5228M5SYJRNzMGsmZsnEUGay5LE3Dwum2kP7d-gxhAy8XYHOBmOvo0vm6kd24ITqMke4EB9XAvKXzQ6iSY2DoYHWRWhG0wb37xv-ACyZplk</recordid><startdate>20110415</startdate><enddate>20110415</enddate><creator>Mochizuki, Hiroyuki</creator><creator>Takahashi, Masashi</creator><creator>Nishigaki, Kazuo</creator><creator>Ide, Tetsuya</creator><creator>Goto-Koshino, Yuko</creator><creator>Watanabe, Shinya</creator><creator>Sato, Hirofumi</creator><creator>Sato, Masahiko</creator><creator>Kotera, Yukiko</creator><creator>Fujino, Yasuhito</creator><creator>Ohno, Koichi</creator><creator>Uchida, Kazuyuki</creator><creator>Tsujimoto, Hajime</creator><general>Elsevier B.V</general><general>Amsterdam: Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20110415</creationdate><title>Establishment of a novel feline leukemia virus (FeLV)-negative B-cell cell line from a cat with B-cell lymphoma</title><author>Mochizuki, Hiroyuki ; Takahashi, Masashi ; Nishigaki, Kazuo ; Ide, Tetsuya ; Goto-Koshino, Yuko ; Watanabe, Shinya ; Sato, Hirofumi ; Sato, Masahiko ; Kotera, Yukiko ; Fujino, Yasuhito ; Ohno, Koichi ; Uchida, Kazuyuki ; Tsujimoto, Hajime</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-3b954683afdd28239a303aa7ca2990476e8eb98324011572b5e832213ede48893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>B-cell</topic><topic>B-Lymphocytes - immunology</topic><topic>B-Lymphocytes - virology</topic><topic>Base Sequence</topic><topic>Cat</topic><topic>Cat Diseases - genetics</topic><topic>Cat Diseases - immunology</topic><topic>Cat Diseases - virology</topic><topic>Cats</topic><topic>Cell Line, Tumor</topic><topic>DNA, Viral - genetics</topic><topic>DNA, Viral - isolation & purification</topic><topic>Feline leukemia virus</topic><topic>FeLV</topic><topic>Gene Rearrangement, B-Lymphocyte, Heavy Chain</topic><topic>Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor</topic><topic>Immunophenotyping</topic><topic>Leukemia Virus, Feline - genetics</topic><topic>Leukemia Virus, Feline - isolation & purification</topic><topic>Lymphoma</topic><topic>Lymphoma, B-Cell - genetics</topic><topic>Lymphoma, B-Cell - immunology</topic><topic>Lymphoma, B-Cell - veterinary</topic><topic>Lymphoma, B-Cell - virology</topic><topic>Male</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mochizuki, Hiroyuki</creatorcontrib><creatorcontrib>Takahashi, Masashi</creatorcontrib><creatorcontrib>Nishigaki, Kazuo</creatorcontrib><creatorcontrib>Ide, Tetsuya</creatorcontrib><creatorcontrib>Goto-Koshino, Yuko</creatorcontrib><creatorcontrib>Watanabe, Shinya</creatorcontrib><creatorcontrib>Sato, Hirofumi</creatorcontrib><creatorcontrib>Sato, Masahiko</creatorcontrib><creatorcontrib>Kotera, Yukiko</creatorcontrib><creatorcontrib>Fujino, Yasuhito</creatorcontrib><creatorcontrib>Ohno, Koichi</creatorcontrib><creatorcontrib>Uchida, Kazuyuki</creatorcontrib><creatorcontrib>Tsujimoto, Hajime</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mochizuki, Hiroyuki</au><au>Takahashi, Masashi</au><au>Nishigaki, Kazuo</au><au>Ide, Tetsuya</au><au>Goto-Koshino, Yuko</au><au>Watanabe, Shinya</au><au>Sato, Hirofumi</au><au>Sato, Masahiko</au><au>Kotera, Yukiko</au><au>Fujino, Yasuhito</au><au>Ohno, Koichi</au><au>Uchida, Kazuyuki</au><au>Tsujimoto, Hajime</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of a novel feline leukemia virus (FeLV)-negative B-cell cell line from a cat with B-cell lymphoma</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2011-04-15</date><risdate>2011</risdate><volume>140</volume><issue>3-4</issue><spage>307</spage><epage>311</epage><pages>307-311</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>We established a novel feline B-cell line, MS4, from the neoplastic pleural effusion of a cat with cutaneous B-cell lymphoma. Immunophenotype staining of the MS4 cells was positive for CD20, CD79α, and IgA and negative for CD3, CD4, CD5, CD8α, CD18, CD21, CD22, IgM, IgG, Ig light chain, and MHC class II. PCR analysis for immunoglobulin heavy chain gene rearrangements revealed a monoclonal rearrangement, whereas no clonal rearrangement of the T-cell receptor γ gene was detected. Southern blotting with an exogenous feline leukemia virus (FeLV) U3 probe revealed no integration of exogenous FeLV provirus. The MS4 cell line is the first FeLV-negative feline B-cell lymphoma cell line, and may be used to investigate the pathogenesis of spontaneously occurring feline lymphoma and the development of new therapies.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>21272942</pmid><doi>10.1016/j.vetimm.2010.12.010</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals B-cell B-Lymphocytes - immunology B-Lymphocytes - virology Base Sequence Cat Cat Diseases - genetics Cat Diseases - immunology Cat Diseases - virology Cats Cell Line, Tumor DNA, Viral - genetics DNA, Viral - isolation & purification Feline leukemia virus FeLV Gene Rearrangement, B-Lymphocyte, Heavy Chain Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor Immunophenotyping Leukemia Virus, Feline - genetics Leukemia Virus, Feline - isolation & purification Lymphoma Lymphoma, B-Cell - genetics Lymphoma, B-Cell - immunology Lymphoma, B-Cell - veterinary Lymphoma, B-Cell - virology Male |
| title | Establishment of a novel feline leukemia virus (FeLV)-negative B-cell cell line from a cat with B-cell lymphoma |
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