Granulocyte-colony stimulating factor ameliorates irradiation-induced suppression of hippocampal neurogenesis in adult mice
▶ Granulocyte-colony stimulating factor (G-csf) demonstrates neuroprotective functions in neurodegenerative diseases. ▶ Cranial irradiation induces neural apoptosis and inhibits neurogenesis in the dentate gyrus of the adult mouse hippocampus. ▶ Pretreatment with G-csf attenuated neural apoptosis an...
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| Veröffentlicht in: | Neuroscience letters 2010-12, Vol.486 (1), p.43-46 |
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| creator | Kim, Joong-Sun Yang, Miyoung Jang, Hyosun Oui, Heejin Kim, Sung-Ho Shin, Taekyun Jang, Won-Suk Lee, Seung-Sook Moon, Changjong |
| description | ▶ Granulocyte-colony stimulating factor (G-csf) demonstrates neuroprotective functions in neurodegenerative diseases. ▶ Cranial irradiation induces neural apoptosis and inhibits neurogenesis in the dentate gyrus of the adult mouse hippocampus. ▶ Pretreatment with G-csf attenuated neural apoptosis and decreased rate of neurogenesis in the DG of the irradiated mouse hippocampus. ▶ The neuroprotective effects of G-csf observed in this study are consistent with similar observations in other brain injury models ▶ G-csf inhibits the detrimental effects of irradiation on hippocampal neurogenesis, suggesting that G-csf administration has potential therapeutic utility in brain irradiation.
Granulocyte-colony stimulating factor (G-csf) is a member of the hematopoietic growth factor family and demonstrates neuroprotective functions in neurodegenerative diseases. This study evaluated the radioprotective effects of G-csf in the suppression of hippocampal neurogenesis in adult mice undergoing irradiation. The radioprotective effects were assessed using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay and immunohistochemical markers of neurogenesis, including the proliferating cell marker Ki-67 and the immature progenitor neuron marker doublecortin (DCX). Acute exposure to cranial irradiation (5
Gy γ-rays) induced neural apoptosis and inhibited neurogenesis in the dentate gyrus (DG) of the adult mouse hippocampus. Pretreatment with G-csf (100
μg/kg every 12
h subcutaneously on three consecutive days) attenuated neural apoptosis and decreased the number of Ki-67- and DCX-positive cells in the DG of the irradiated mouse hippocampus. Therefore, G-csf inhibited the detrimental effects of irradiation on hippocampal neurogenesis, suggesting that G-csf administration has potential therapeutic utility in brain irradiation. |
| doi_str_mv | 10.1016/j.neulet.2010.09.041 |
| format | Article |
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Granulocyte-colony stimulating factor (G-csf) is a member of the hematopoietic growth factor family and demonstrates neuroprotective functions in neurodegenerative diseases. This study evaluated the radioprotective effects of G-csf in the suppression of hippocampal neurogenesis in adult mice undergoing irradiation. The radioprotective effects were assessed using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay and immunohistochemical markers of neurogenesis, including the proliferating cell marker Ki-67 and the immature progenitor neuron marker doublecortin (DCX). Acute exposure to cranial irradiation (5
Gy γ-rays) induced neural apoptosis and inhibited neurogenesis in the dentate gyrus (DG) of the adult mouse hippocampus. Pretreatment with G-csf (100
μg/kg every 12
h subcutaneously on three consecutive days) attenuated neural apoptosis and decreased the number of Ki-67- and DCX-positive cells in the DG of the irradiated mouse hippocampus. Therefore, G-csf inhibited the detrimental effects of irradiation on hippocampal neurogenesis, suggesting that G-csf administration has potential therapeutic utility in brain irradiation.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2010.09.041</identifier><identifier>PMID: 20854880</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Apoptosis - drug effects ; Apoptosis - radiation effects ; Biological and medical sciences ; Cranial Irradiation - adverse effects ; Dentate Gyrus - drug effects ; Dentate Gyrus - metabolism ; Dentate Gyrus - radiation effects ; Fundamental and applied biological sciences. Psychology ; G-csf ; Granulocyte Colony-Stimulating Factor - pharmacology ; Hippocampus ; Hippocampus - cytology ; Hippocampus - drug effects ; Hippocampus - metabolism ; Hippocampus - radiation effects ; Irradiation ; Ki-67 Antigen - metabolism ; Male ; Mice ; Mice, Inbred C3H ; Microtubule-Associated Proteins - metabolism ; Neurogenesis ; Neurogenesis - drug effects ; Neuropeptides - metabolism ; Neuroprotective Agents - pharmacology ; Radiation-Protective Agents - pharmacology ; Recombinant Proteins ; Vertebrates: nervous system and sense organs</subject><ispartof>Neuroscience letters, 2010-12, Vol.486 (1), p.43-46</ispartof><rights>2010 Elsevier Ireland Ltd</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-601ccc100bddadaa0b9a4290b047ceb1d5d171953df695f83b609eb1355674063</citedby><cites>FETCH-LOGICAL-c455t-601ccc100bddadaa0b9a4290b047ceb1d5d171953df695f83b609eb1355674063</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304394010012589$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23377961$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20854880$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Joong-Sun</creatorcontrib><creatorcontrib>Yang, Miyoung</creatorcontrib><creatorcontrib>Jang, Hyosun</creatorcontrib><creatorcontrib>Oui, Heejin</creatorcontrib><creatorcontrib>Kim, Sung-Ho</creatorcontrib><creatorcontrib>Shin, Taekyun</creatorcontrib><creatorcontrib>Jang, Won-Suk</creatorcontrib><creatorcontrib>Lee, Seung-Sook</creatorcontrib><creatorcontrib>Moon, Changjong</creatorcontrib><title>Granulocyte-colony stimulating factor ameliorates irradiation-induced suppression of hippocampal neurogenesis in adult mice</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>▶ Granulocyte-colony stimulating factor (G-csf) demonstrates neuroprotective functions in neurodegenerative diseases. ▶ Cranial irradiation induces neural apoptosis and inhibits neurogenesis in the dentate gyrus of the adult mouse hippocampus. ▶ Pretreatment with G-csf attenuated neural apoptosis and decreased rate of neurogenesis in the DG of the irradiated mouse hippocampus. ▶ The neuroprotective effects of G-csf observed in this study are consistent with similar observations in other brain injury models ▶ G-csf inhibits the detrimental effects of irradiation on hippocampal neurogenesis, suggesting that G-csf administration has potential therapeutic utility in brain irradiation.
Granulocyte-colony stimulating factor (G-csf) is a member of the hematopoietic growth factor family and demonstrates neuroprotective functions in neurodegenerative diseases. This study evaluated the radioprotective effects of G-csf in the suppression of hippocampal neurogenesis in adult mice undergoing irradiation. The radioprotective effects were assessed using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay and immunohistochemical markers of neurogenesis, including the proliferating cell marker Ki-67 and the immature progenitor neuron marker doublecortin (DCX). Acute exposure to cranial irradiation (5
Gy γ-rays) induced neural apoptosis and inhibited neurogenesis in the dentate gyrus (DG) of the adult mouse hippocampus. Pretreatment with G-csf (100
μg/kg every 12
h subcutaneously on three consecutive days) attenuated neural apoptosis and decreased the number of Ki-67- and DCX-positive cells in the DG of the irradiated mouse hippocampus. Therefore, G-csf inhibited the detrimental effects of irradiation on hippocampal neurogenesis, suggesting that G-csf administration has potential therapeutic utility in brain irradiation.</description><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - radiation effects</subject><subject>Biological and medical sciences</subject><subject>Cranial Irradiation - adverse effects</subject><subject>Dentate Gyrus - drug effects</subject><subject>Dentate Gyrus - metabolism</subject><subject>Dentate Gyrus - radiation effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>G-csf</subject><subject>Granulocyte Colony-Stimulating Factor - pharmacology</subject><subject>Hippocampus</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - metabolism</subject><subject>Hippocampus - radiation effects</subject><subject>Irradiation</subject><subject>Ki-67 Antigen - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Microtubule-Associated Proteins - metabolism</subject><subject>Neurogenesis</subject><subject>Neurogenesis - drug effects</subject><subject>Neuropeptides - metabolism</subject><subject>Neuroprotective Agents - pharmacology</subject><subject>Radiation-Protective Agents - pharmacology</subject><subject>Recombinant Proteins</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGL1DAYhoso7rj6D0RyEU8dvzRJ01wEWXQVFrzoOaTJ1zVDmtSkFQb_vBlm1Jt7Cnx5vjcveZrmJYU9Bdq_PewjbgHXfQd1BGoPnD5qdnSQXSuV7B43O2DAW6Y4XDXPSjkAgKCCP22uOhgEHwbYNb9us4lbSPa4YmtTSPFIyurnLZjVx3syGbumTMyMwadsVizE52ycr9cptj66zaIjZVuWjKXUGUkT-e6XJVkzLyaQ2jKne4xYfN2NxLgtrGT2Fp83TyYTCr64nNfNt48fvt58au--3H6-eX_XWi7E2vZArbUUYHTOOGNgVIZ3Ckbg0uJInXBUUiWYm3olpoGNPag6Z0L0kkPPrps359wlpx8bllXPvlgMwURMW9FDLyVTig4PkzVRqqGTD5JSKMoABlpJfiZtTqVknPSS_WzyUVPQJ5P6oM8m9cmkBqWrybr26vLANs7o_i79UVeB1xfAFGvCVD1aX_5xjNWq_Sno3ZnD-sU_PWZdrMdYrfmMdtUu-f83-Q35XcEN</recordid><startdate>20101203</startdate><enddate>20101203</enddate><creator>Kim, Joong-Sun</creator><creator>Yang, Miyoung</creator><creator>Jang, Hyosun</creator><creator>Oui, Heejin</creator><creator>Kim, Sung-Ho</creator><creator>Shin, Taekyun</creator><creator>Jang, Won-Suk</creator><creator>Lee, Seung-Sook</creator><creator>Moon, Changjong</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>20101203</creationdate><title>Granulocyte-colony stimulating factor ameliorates irradiation-induced suppression of hippocampal neurogenesis in adult mice</title><author>Kim, Joong-Sun ; Yang, Miyoung ; Jang, Hyosun ; Oui, Heejin ; Kim, Sung-Ho ; Shin, Taekyun ; Jang, Won-Suk ; Lee, Seung-Sook ; Moon, Changjong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-601ccc100bddadaa0b9a4290b047ceb1d5d171953df695f83b609eb1355674063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - radiation effects</topic><topic>Biological and medical sciences</topic><topic>Cranial Irradiation - adverse effects</topic><topic>Dentate Gyrus - drug effects</topic><topic>Dentate Gyrus - metabolism</topic><topic>Dentate Gyrus - radiation effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>G-csf</topic><topic>Granulocyte Colony-Stimulating Factor - pharmacology</topic><topic>Hippocampus</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - metabolism</topic><topic>Hippocampus - radiation effects</topic><topic>Irradiation</topic><topic>Ki-67 Antigen - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Microtubule-Associated Proteins - metabolism</topic><topic>Neurogenesis</topic><topic>Neurogenesis - drug effects</topic><topic>Neuropeptides - metabolism</topic><topic>Neuroprotective Agents - pharmacology</topic><topic>Radiation-Protective Agents - pharmacology</topic><topic>Recombinant Proteins</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Joong-Sun</creatorcontrib><creatorcontrib>Yang, Miyoung</creatorcontrib><creatorcontrib>Jang, Hyosun</creatorcontrib><creatorcontrib>Oui, Heejin</creatorcontrib><creatorcontrib>Kim, Sung-Ho</creatorcontrib><creatorcontrib>Shin, Taekyun</creatorcontrib><creatorcontrib>Jang, Won-Suk</creatorcontrib><creatorcontrib>Lee, Seung-Sook</creatorcontrib><creatorcontrib>Moon, Changjong</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Joong-Sun</au><au>Yang, Miyoung</au><au>Jang, Hyosun</au><au>Oui, Heejin</au><au>Kim, Sung-Ho</au><au>Shin, Taekyun</au><au>Jang, Won-Suk</au><au>Lee, Seung-Sook</au><au>Moon, Changjong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Granulocyte-colony stimulating factor ameliorates irradiation-induced suppression of hippocampal neurogenesis in adult mice</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2010-12-03</date><risdate>2010</risdate><volume>486</volume><issue>1</issue><spage>43</spage><epage>46</epage><pages>43-46</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>▶ Granulocyte-colony stimulating factor (G-csf) demonstrates neuroprotective functions in neurodegenerative diseases. ▶ Cranial irradiation induces neural apoptosis and inhibits neurogenesis in the dentate gyrus of the adult mouse hippocampus. ▶ Pretreatment with G-csf attenuated neural apoptosis and decreased rate of neurogenesis in the DG of the irradiated mouse hippocampus. ▶ The neuroprotective effects of G-csf observed in this study are consistent with similar observations in other brain injury models ▶ G-csf inhibits the detrimental effects of irradiation on hippocampal neurogenesis, suggesting that G-csf administration has potential therapeutic utility in brain irradiation.
Granulocyte-colony stimulating factor (G-csf) is a member of the hematopoietic growth factor family and demonstrates neuroprotective functions in neurodegenerative diseases. This study evaluated the radioprotective effects of G-csf in the suppression of hippocampal neurogenesis in adult mice undergoing irradiation. The radioprotective effects were assessed using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay and immunohistochemical markers of neurogenesis, including the proliferating cell marker Ki-67 and the immature progenitor neuron marker doublecortin (DCX). Acute exposure to cranial irradiation (5
Gy γ-rays) induced neural apoptosis and inhibited neurogenesis in the dentate gyrus (DG) of the adult mouse hippocampus. Pretreatment with G-csf (100
μg/kg every 12
h subcutaneously on three consecutive days) attenuated neural apoptosis and decreased the number of Ki-67- and DCX-positive cells in the DG of the irradiated mouse hippocampus. Therefore, G-csf inhibited the detrimental effects of irradiation on hippocampal neurogenesis, suggesting that G-csf administration has potential therapeutic utility in brain irradiation.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>20854880</pmid><doi>10.1016/j.neulet.2010.09.041</doi><tpages>4</tpages></addata></record> |
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| subjects | Animals Apoptosis - drug effects Apoptosis - radiation effects Biological and medical sciences Cranial Irradiation - adverse effects Dentate Gyrus - drug effects Dentate Gyrus - metabolism Dentate Gyrus - radiation effects Fundamental and applied biological sciences. Psychology G-csf Granulocyte Colony-Stimulating Factor - pharmacology Hippocampus Hippocampus - cytology Hippocampus - drug effects Hippocampus - metabolism Hippocampus - radiation effects Irradiation Ki-67 Antigen - metabolism Male Mice Mice, Inbred C3H Microtubule-Associated Proteins - metabolism Neurogenesis Neurogenesis - drug effects Neuropeptides - metabolism Neuroprotective Agents - pharmacology Radiation-Protective Agents - pharmacology Recombinant Proteins Vertebrates: nervous system and sense organs |
| title | Granulocyte-colony stimulating factor ameliorates irradiation-induced suppression of hippocampal neurogenesis in adult mice |
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